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The map label for this gene is ksgA

Identifier: 15669218

GI number: 15669218

Start: 961661

End: 962488

Strand: Direct

Name: ksgA

Synonym: MJ1029

Alternate gene names: 15669218

Gene position: 961661-962488 (Clockwise)

Preceding gene: 15669217

Following gene: 15669219

Centisome position: 57.76

GC content: 29.95

Gene sequence:

>828_bases
ATGTTCAAACCAAAGAAAAAATTAGGGCAATGCTTTTTAATAGATAAGAATTTTGTTAATAAGGCAGTGGAATCTGCAAA
TCTTACAAAGGATGATGTAGTTTTAGAGATTGGCTTAGGAAAAGGAATTTTAACAGAAGAGCTTGCTAAAAATGCTAAAA
AAGTCTATGTCATTGAGATAGATAAAAGCTTAGAGCCTTATGCTAATAAATTAAAAGAGCTTTATAACAATATAGAAATC
ATCTGGGGAGATGCATTAAAAGTTGATTTAAACAAGTTAGATTTTAATAAGGTTGTAGCTAATCTTCCATATCAAATTTC
ATCACCAATAACATTTAAATTGATAAAGAGGGGCTTTGATTTAGCTGTTTTAATGTATCAGTATGAGTTTGCCAAGAGAA
TGGTAGCTAAAGAGGGAACAAAAGATTATGGAAGGTTATCTGTGGCGGTTCAATCAAGGGCAGATGTTGAGATAGTAGCT
AAAGTTCCACCAAGTGCTTTCTATCCAAAACCAAAGGTTTATTCTGCAATAGTTAAAATAAAACCTAACAAAGGCAAATA
CCACATAGAGAATGAAAATTTCTTTGATGATTTTTTGAGAGCTATATTTCAACATAGAAATAAATCAGTTAGGAAGGCGT
TGATTGATTCTTCCAAAGAATTAAACTATAACAAAGATGAAATGAAAAAGATATTGGAAGATTTTTTAAATACAAACTCA
GAGATAAAAAACCTAATAAATGAGAAGGTTTTTAAACTCTCTGTTAAAGATATAGTTAATCTCTCAAATGAATTCTATAG
ATTTCTGCAAAATAGGGGTAGGTTATAG

Upstream 100 bases:

>100_bases
TAAATAAACCAGTTGATGAGATTGAAGAGAAAGTTGTTAAGTTGTTAGAATAAATTTTTATTTTCTTTTCTCTTTTTTAG
AATAAATAAAAAGTGAAACA

Downstream 100 bases:

>100_bases
CCATGAAGGTAGTAGTAGGAGGAACATTTGATATTCTGCATAGAGGGCATAAGGAGCTTTTAAAATTTGCATCCTCTTTA
GGAAAATTAACTATAGGAAT

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N',N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MFKPKKKLGQCFLIDKNFVNKAVESANLTKDDVVLEIGLGKGILTEELAKNAKKVYVIEIDKSLEPYANKLKELYNNIEI
IWGDALKVDLNKLDFNKVVANLPYQISSPITFKLIKRGFDLAVLMYQYEFAKRMVAKEGTKDYGRLSVAVQSRADVEIVA
KVPPSAFYPKPKVYSAIVKIKPNKGKYHIENENFFDDFLRAIFQHRNKSVRKALIDSSKELNYNKDEMKKILEDFLNTNS
EIKNLINEKVFKLSVKDIVNLSNEFYRFLQNRGRL

Sequences:

>Translated_275_residues
MFKPKKKLGQCFLIDKNFVNKAVESANLTKDDVVLEIGLGKGILTEELAKNAKKVYVIEIDKSLEPYANKLKELYNNIEI
IWGDALKVDLNKLDFNKVVANLPYQISSPITFKLIKRGFDLAVLMYQYEFAKRMVAKEGTKDYGRLSVAVQSRADVEIVA
KVPPSAFYPKPKVYSAIVKIKPNKGKYHIENENFFDDFLRAIFQHRNKSVRKALIDSSKELNYNKDEMKKILEDFLNTNS
EIKNLINEKVFKLSVKDIVNLSNEFYRFLQNRGRL
>Mature_275_residues
MFKPKKKLGQCFLIDKNFVNKAVESANLTKDDVVLEIGLGKGILTEELAKNAKKVYVIEIDKSLEPYANKLKELYNNIEI
IWGDALKVDLNKLDFNKVVANLPYQISSPITFKLIKRGFDLAVLMYQYEFAKRMVAKEGTKDYGRLSVAVQSRADVEIVA
KVPPSAFYPKPKVYSAIVKIKPNKGKYHIENENFFDDFLRAIFQHRNKSVRKALIDSSKELNYNKDEMKKILEDFLNTNS
EIKNLINEKVFKLSVKDIVNLSNEFYRFLQNRGRL

Specific function: Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI7657198, Length=252, Percent_Identity=35.7142857142857, Blast_Score=134, Evalue=9e-32,
Organism=Homo sapiens, GI156415992, Length=235, Percent_Identity=26.3829787234043, Blast_Score=78, Evalue=9e-15,
Organism=Escherichia coli, GI1786236, Length=218, Percent_Identity=33.4862385321101, Blast_Score=113, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI25146882, Length=209, Percent_Identity=41.6267942583732, Blast_Score=127, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI25141369, Length=243, Percent_Identity=27.1604938271605, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6324989, Length=247, Percent_Identity=32.7935222672065, Blast_Score=119, Evalue=6e-28,
Organism=Drosophila melanogaster, GI21358017, Length=210, Percent_Identity=39.5238095238095, Blast_Score=129, Evalue=1e-30,
Organism=Drosophila melanogaster, GI21357273, Length=289, Percent_Identity=25.2595155709343, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_METJA (Q58435)

Other databases:

- EMBL:   L77117
- PIR:   D64428
- RefSeq:   NP_248023.1
- PDB:   3FYC
- PDB:   3FYD
- PDB:   3GRR
- PDB:   3GRU
- PDB:   3GRV
- PDB:   3GRY
- PDBsum:   3FYC
- PDBsum:   3FYD
- PDBsum:   3GRR
- PDBsum:   3GRU
- PDBsum:   3GRV
- PDBsum:   3GRY
- ProteinModelPortal:   Q58435
- SMR:   Q58435
- GeneID:   1451926
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1029
- NMPDR:   fig|243232.1.peg.1060
- TIGR:   MJ1029
- HOGENOM:   HBG319664
- OMA:   LAVLMYQ
- ProtClustDB:   PRK14896
- BioCyc:   MJAN243232:MJ_1029-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: 2.1.1.- [C]

Molecular weight: Translated: 31771; Mature: 31771

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 13-13 BINDING 38-38 BINDING 59-59 BINDING 84-84 BINDING 101-101

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKPKKKLGQCFLIDKNFVNKAVESANLTKDDVVLEIGLGKGILTEELAKNAKKVYVIEI
CCCCCHHHCCEEEEEHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHCCCCEEEEEEE
DKSLEPYANKLKELYNNIEIIWGDALKVDLNKLDFNKVVANLPYQISSPITFKLIKRGFD
CCCCCHHHHHHHHHHCCCEEEECCEEEEEHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCC
LAVLMYQYEFAKRMVAKEGTKDYGRLSVAVQSRADVEIVAKVPPSAFYPKPKVYSAIVKI
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEECCCCCCCCCCHHEEEEEEE
KPNKGKYHIENENFFDDFLRAIFQHRNKSVRKALIDSSKELNYNKDEMKKILEDFLNTNS
ECCCCCEEECCCCHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCH
EIKNLINEKVFKLSVKDIVNLSNEFYRFLQNRGRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFKPKKKLGQCFLIDKNFVNKAVESANLTKDDVVLEIGLGKGILTEELAKNAKKVYVIEI
CCCCCHHHCCEEEEEHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHCCCCEEEEEEE
DKSLEPYANKLKELYNNIEIIWGDALKVDLNKLDFNKVVANLPYQISSPITFKLIKRGFD
CCCCCHHHHHHHHHHCCCEEEECCEEEEEHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCC
LAVLMYQYEFAKRMVAKEGTKDYGRLSVAVQSRADVEIVAKVPPSAFYPKPKVYSAIVKI
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEECCCCCCCCCCHHEEEEEEE
KPNKGKYHIENENFFDDFLRAIFQHRNKSVRKALIDSSKELNYNKDEMKKILEDFLNTNS
ECCCCCEEECCCCHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCH
EIKNLINEKVFKLSVKDIVNLSNEFYRFLQNRGRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Dimethylation [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087