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The map label for this gene is hycE [C]

Identifier: 15669216

GI number: 15669216

Start: 958359

End: 959492

Strand: Direct

Name: hycE [C]

Synonym: MJ1027

Alternate gene names: 15669216

Gene position: 958359-959492 (Clockwise)

Preceding gene: 15669213

Following gene: 15669217

Centisome position: 57.56

GC content: 35.54

Gene sequence:

>1134_bases
GTGTTGTTCATGAAGTATGAGGGAGAGATAGCTATAGGTCCAGTTCATCCTACAATGCTTGAACCACATAGGTTAAGATT
GTTTATTGAAGATGAAATTATAAAAGAGGCTGAATTAGTAATTGGTGTTAATTATAGGGGAATTGAGCTTATCATGGAAG
GATTGCCACCAGAAAAAATTAGTATATTATCTGAAAAGATTTGTGGTATTTGCTCACATATTCATGTATGGTGTAACGTT
ACAGTTACTGAGAGAGGATGTGATATAGAGGTTCCAGAGAGAGCTGAGTATATAAGGGCTATAGTTGAAGAGTTGGAGAG
ATTACATTCCCACATGTTGCTGTTTGGACATGCCTTTGAGGTTTTAGGCTTTGAAACAATGGCTTTCAGAGCTTTTATGA
TTAGAGAACCAATAATGCAAATATTGGGAGAGATTACTGGAGGGAGGGCTCAATACTCTTGCCCTATAATAGGAGGAATT
AGGCCGAGATGTAATATAAAAGAGAGCAAGATTCCAGCTTTATTAGAGAGGTTGGAAAAGTTTGAAGAAAATTTAAAGAA
ATTATTGGAAAGAACCGTTAATGACCCAATGATTATGTCAAGGATTAAGGATGTTGGTGTTTTAGATAAAAAAACTGCTA
AAAAACTCCATGCTCTTGGCCCAACAGCGAGAGGTTCTGGAATACATAGTGACATGAGAAAGATGGGACAAGTCCCTGTT
TATGATAACTTTGAGTTTGAAGAGATTGTGTTTGATGATGGAGATGTATTTAGCAGATTGGCAGTTAGATTTTATGAGTG
TTTTGAGAGCGTTAAAATCATTAGACAGGGTTTAAAGGACTTGCCAAATTTGGATAAAAGAATATATAACCCAAACTATG
AATTAAAGGAGTTTAAACCAATAGATGTTTATAATGAAGCTCAAAGAGGGCAGGTTTATTATTCTTATGGTTTAGATGAA
AATGGTAGAGTTAGACAGGTAAAAATTAGGACACCAACAGCAACCAACTTAGCTTGTATGGAAGCAATTCTGCCAGGACA
TCATGTTTCAGATGCTGAATTAATTATAGCAAGCTGTGACCCATGCTTTACTTGCACAGATAGGATGATTGTTGTTAAAG
AAAATAAGATTTAA

Upstream 100 bases:

>100_bases
TATATAAAAGGTTAAGCTAATTATCATTATTAATTATGATAAATTATCTATTCATTACCTTAGTGAGTCATATACCTATT
ATCATGTTTATAATTGTTGG

Downstream 100 bases:

>100_bases
AGTTTAAAAATAGTTTAGTTAATTTCTATTTTGTTACATTATTTATTTTACTGATATTTATTGATAAATATAAATATGAC
AATTTTAAATTTTATATATC

Product: formate hydrogenlyase subunit 5

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: NA

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MLFMKYEGEIAIGPVHPTMLEPHRLRLFIEDEIIKEAELVIGVNYRGIELIMEGLPPEKISILSEKICGICSHIHVWCNV
TVTERGCDIEVPERAEYIRAIVEELERLHSHMLLFGHAFEVLGFETMAFRAFMIREPIMQILGEITGGRAQYSCPIIGGI
RPRCNIKESKIPALLERLEKFEENLKKLLERTVNDPMIMSRIKDVGVLDKKTAKKLHALGPTARGSGIHSDMRKMGQVPV
YDNFEFEEIVFDDGDVFSRLAVRFYECFESVKIIRQGLKDLPNLDKRIYNPNYELKEFKPIDVYNEAQRGQVYYSYGLDE
NGRVRQVKIRTPTATNLACMEAILPGHHVSDAELIIASCDPCFTCTDRMIVVKENKI

Sequences:

>Translated_377_residues
MLFMKYEGEIAIGPVHPTMLEPHRLRLFIEDEIIKEAELVIGVNYRGIELIMEGLPPEKISILSEKICGICSHIHVWCNV
TVTERGCDIEVPERAEYIRAIVEELERLHSHMLLFGHAFEVLGFETMAFRAFMIREPIMQILGEITGGRAQYSCPIIGGI
RPRCNIKESKIPALLERLEKFEENLKKLLERTVNDPMIMSRIKDVGVLDKKTAKKLHALGPTARGSGIHSDMRKMGQVPV
YDNFEFEEIVFDDGDVFSRLAVRFYECFESVKIIRQGLKDLPNLDKRIYNPNYELKEFKPIDVYNEAQRGQVYYSYGLDE
NGRVRQVKIRTPTATNLACMEAILPGHHVSDAELIIASCDPCFTCTDRMIVVKENKI
>Mature_377_residues
MLFMKYEGEIAIGPVHPTMLEPHRLRLFIEDEIIKEAELVIGVNYRGIELIMEGLPPEKISILSEKICGICSHIHVWCNV
TVTERGCDIEVPERAEYIRAIVEELERLHSHMLLFGHAFEVLGFETMAFRAFMIREPIMQILGEITGGRAQYSCPIIGGI
RPRCNIKESKIPALLERLEKFEENLKKLLERTVNDPMIMSRIKDVGVLDKKTAKKLHALGPTARGSGIHSDMRKMGQVPV
YDNFEFEEIVFDDGDVFSRLAVRFYECFESVKIIRQGLKDLPNLDKRIYNPNYELKEFKPIDVYNEAQRGQVYYSYGLDE
NGRVRQVKIRTPTATNLACMEAILPGHHVSDAELIIASCDPCFTCTDRMIVVKENKI

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 49 kDa subunit family

Homologues:

Organism=Homo sapiens, GI4758786, Length=393, Percent_Identity=25.9541984732824, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI260898743, Length=384, Percent_Identity=25.78125, Blast_Score=100, Evalue=3e-21,
Organism=Escherichia coli, GI1789076, Length=368, Percent_Identity=33.695652173913, Blast_Score=192, Evalue=2e-50,
Organism=Escherichia coli, GI1788832, Length=368, Percent_Identity=32.0652173913043, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI145693162, Length=385, Percent_Identity=24.4155844155844, Blast_Score=99, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17568379, Length=391, Percent_Identity=25.0639386189258, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17555284, Length=389, Percent_Identity=24.4215938303342, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI221459469, Length=384, Percent_Identity=26.3020833333333, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24638644, Length=385, Percent_Identity=25.974025974026, Blast_Score=111, Evalue=9e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y1027_METJA (Q58433)

Other databases:

- EMBL:   L77117
- PIR:   B64428
- RefSeq:   NP_248021.1
- ProteinModelPortal:   Q58433
- GeneID:   1451924
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1027
- NMPDR:   fig|243232.1.peg.1058
- TIGR:   MJ1027
- HOGENOM:   HBG459705
- OMA:   CFTCTDR
- ProtClustDB:   CLSK876431
- BioCyc:   MJAN243232:MJ_1027-MONOMER
- InterPro:   IPR001135
- InterPro:   IPR001501
- InterPro:   IPR018194

Pfam domain/function: PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases

EC number: NA

Molecular weight: Translated: 43176; Mature: 43176

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00535 COMPLEX1_49K; PS00507 NI_HGENASE_L_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFMKYEGEIAIGPVHPTMLEPHRLRLFIEDEIIKEAELVIGVNYRGIELIMEGLPPEKI
CEEEEECCCEEECCCCCCCCCCCCEEEEEHHHHHCCEEEEEECCCCHHHHHHHCCCCHHH
SILSEKICGICSHIHVWCNVTVTERGCDIEVPERAEYIRAIVEELERLHSHMLLFGHAFE
HHHHHHHHHHHHCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLGFETMAFRAFMIREPIMQILGEITGGRAQYSCPIIGGIRPRCNIKESKIPALLERLEK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCEECCCCCCCCCCHHHHHHHHHHHHH
FEENLKKLLERTVNDPMIMSRIKDVGVLDKKTAKKLHALGPTARGSGIHSDMRKMGQVPV
HHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCC
YDNFEFEEIVFDDGDVFSRLAVRFYECFESVKIIRQGLKDLPNLDKRIYNPNYELKEFKP
CCCCCHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCC
IDVYNEAQRGQVYYSYGLDENGRVRQVKIRTPTATNLACMEAILPGHHVSDAELIIASCD
CCCCCCCCCCEEEEEECCCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCCCCEEEEECCC
PCFTCTDRMIVVKENKI
CCEECCCCEEEEECCCC
>Mature Secondary Structure
MLFMKYEGEIAIGPVHPTMLEPHRLRLFIEDEIIKEAELVIGVNYRGIELIMEGLPPEKI
CEEEEECCCEEECCCCCCCCCCCCEEEEEHHHHHCCEEEEEECCCCHHHHHHHCCCCHHH
SILSEKICGICSHIHVWCNVTVTERGCDIEVPERAEYIRAIVEELERLHSHMLLFGHAFE
HHHHHHHHHHHHCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLGFETMAFRAFMIREPIMQILGEITGGRAQYSCPIIGGIRPRCNIKESKIPALLERLEK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCEECCCCCCCCCCHHHHHHHHHHHHH
FEENLKKLLERTVNDPMIMSRIKDVGVLDKKTAKKLHALGPTARGSGIHSDMRKMGQVPV
HHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCC
YDNFEFEEIVFDDGDVFSRLAVRFYECFESVKIIRQGLKDLPNLDKRIYNPNYELKEFKP
CCCCCHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCC
IDVYNEAQRGQVYYSYGLDENGRVRQVKIRTPTATNLACMEAILPGHHVSDAELIIASCD
CCCCCCCCCCEEEEEECCCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCCCCEEEEECCC
PCFTCTDRMIVVKENKI
CCEECCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087