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The map label for this gene is trm1

Identifier: 15669136

GI number: 15669136

Start: 876317

End: 877441

Strand: Reverse

Name: trm1

Synonym: MJ0946

Alternate gene names: 15669136

Gene position: 877441-876317 (Counterclockwise)

Preceding gene: 15669137

Following gene: 15669135

Centisome position: 52.7

GC content: 32.8

Gene sequence:

>1125_bases
ATGATTCTAAAAGAGGGGGAAGTAGTTTTTGAAGTTCCAGATAAATTGACAGTTACAAAAAAGGATGAGGTATTTTATAA
TCCAAGAATGAAAACATGCAGGGATATAAGTATAGCAGTAATTCAGGCATTTCTAAATTTGTATCATAAGAGAGATAAGT
TTTACATTGCTGATGCTTTGGCTGGAAGTGGAATTAGGGGGCTTAGATACGCTAAAGAGCTTGAGTTTAATGGAGAGTTA
AAGGTTTTTTTAAATGATATAAATCCAAAAGCTTATGAGAAGATAATAAACAATGCCAAATTAAATGAGATTGAGAATAT
AGATGTTTTTAATGAAGATGCCAACACATTTTTATCTAAGCATTTTAGATTTTTTAATGTTGTTGATATAGACCCGTTTG
GCTCTCCAGCTCCTTATGTAGAGCAAGCAATTAGGGCTTTAGTAACAAGAAATGGTTTGCTCTGTTTAACAGCAACAGAT
ACAGCGGCATTATGTGGTAGGTCTAAAAAATCATGCTTAAGGAAATACTTGGCTTATCCATTATTTGGTAGGGATTGTCA
TGAATTTGCATTGAGGGTTTTAGTTGGATATGTTATGAGAATGGCTACAAAATATGAGCTTGCTTTAAAGCCAGTATTTT
GCCATGCCACAGACCATTATGTTAGAGTTTATTTAGTTACGGATAGGGGAGCTAAGAGGGCTGATAAGGTTTTTGAAATG
CTTGGCTATGTTAAGGATGTAAATGGAATTAAAATAATTAAAAAATTTGAAGAAGGTTATGAGAAAGGATTTGCTGGGCC
TTTATATATAGGCAATTTGTATGATAAAGCTCTTGTTGAAGAGGCTTTAAAAATAGCTGAAAAGAGGGAGTTTAGTGAAA
GAGTTTTAAAGATTTTAAATGCCATTAAAGGAGAATCTGCTATAAATCAAGTTGGATGTTATGACACTCACCAAATTGGG
AAAATGTTAAAGATTTCAGTTCCACCAATGCAAGATATTATAAACAAGCTAAAAGAGATGGGATTTAATGCAGTTGTAAC
TCACTATAATCCGAAAGGAATAAAAACTGATGCAACATTAAAGAATGTTATTGAGGCAATATATCAATGTACCAAGATTA
GGTGA

Upstream 100 bases:

>100_bases
AAAAATTTCTTTTTAGTTTTCTTTAATACTTTTACTTTTATTTCTATTTTTAATTGTTTTTAATTTATAAAACTAAGACA
ATATTATTCTGTGTGATAAT

Downstream 100 bases:

>100_bases
AATTATGAATCTTAAAGAACTTACTGTAATTTTGATTATCCCAATTGTATATTTGGGAGTGTGTGGTTGTTTTGAAATTG
TCCCAAAATCCTTTTATGAT

Product: N(2),N(2)-dimethylguanosine tRNA methyltransferase

Products: NA

Alternate protein names: tRNA 2,2-dimethylguanosine-26 methyltransferase; tRNA(guanine-26,N(2)-N(2)) methyltransferase; tRNA(m(2,2)G26)dimethyltransferase

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MILKEGEVVFEVPDKLTVTKKDEVFYNPRMKTCRDISIAVIQAFLNLYHKRDKFYIADALAGSGIRGLRYAKELEFNGEL
KVFLNDINPKAYEKIINNAKLNEIENIDVFNEDANTFLSKHFRFFNVVDIDPFGSPAPYVEQAIRALVTRNGLLCLTATD
TAALCGRSKKSCLRKYLAYPLFGRDCHEFALRVLVGYVMRMATKYELALKPVFCHATDHYVRVYLVTDRGAKRADKVFEM
LGYVKDVNGIKIIKKFEEGYEKGFAGPLYIGNLYDKALVEEALKIAEKREFSERVLKILNAIKGESAINQVGCYDTHQIG
KMLKISVPPMQDIINKLKEMGFNAVVTHYNPKGIKTDATLKNVIEAIYQCTKIR

Sequences:

>Translated_374_residues
MILKEGEVVFEVPDKLTVTKKDEVFYNPRMKTCRDISIAVIQAFLNLYHKRDKFYIADALAGSGIRGLRYAKELEFNGEL
KVFLNDINPKAYEKIINNAKLNEIENIDVFNEDANTFLSKHFRFFNVVDIDPFGSPAPYVEQAIRALVTRNGLLCLTATD
TAALCGRSKKSCLRKYLAYPLFGRDCHEFALRVLVGYVMRMATKYELALKPVFCHATDHYVRVYLVTDRGAKRADKVFEM
LGYVKDVNGIKIIKKFEEGYEKGFAGPLYIGNLYDKALVEEALKIAEKREFSERVLKILNAIKGESAINQVGCYDTHQIG
KMLKISVPPMQDIINKLKEMGFNAVVTHYNPKGIKTDATLKNVIEAIYQCTKIR
>Mature_374_residues
MILKEGEVVFEVPDKLTVTKKDEVFYNPRMKTCRDISIAVIQAFLNLYHKRDKFYIADALAGSGIRGLRYAKELEFNGEL
KVFLNDINPKAYEKIINNAKLNEIENIDVFNEDANTFLSKHFRFFNVVDIDPFGSPAPYVEQAIRALVTRNGLLCLTATD
TAALCGRSKKSCLRKYLAYPLFGRDCHEFALRVLVGYVMRMATKYELALKPVFCHATDHYVRVYLVTDRGAKRADKVFEM
LGYVKDVNGIKIIKKFEEGYEKGFAGPLYIGNLYDKALVEEALKIAEKREFSERVLKILNAIKGESAINQVGCYDTHQIG
KMLKISVPPMQDIINKLKEMGFNAVVTHYNPKGIKTDATLKNVIEAIYQCTKIR

Specific function: Dimethylates a single guanine residue at position 26 of a number of tRNAs using S-adenosyl-L-methionine as donor of the methyl groups

COG id: COG1867

COG function: function code J; N2,N2-dimethylguanosine tRNA methyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TRM1 family

Homologues:

Organism=Homo sapiens, GI216548445, Length=329, Percent_Identity=29.1793313069909, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI8923219, Length=356, Percent_Identity=28.0898876404494, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI209862871, Length=356, Percent_Identity=28.0898876404494, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI163792194, Length=267, Percent_Identity=27.7153558052434, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17564890, Length=415, Percent_Identity=26.2650602409639, Blast_Score=142, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6320325, Length=460, Percent_Identity=26.0869565217391, Blast_Score=141, Evalue=1e-34,
Organism=Drosophila melanogaster, GI19921190, Length=419, Percent_Identity=23.6276849642005, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRM1_METJA (Q58356)

Other databases:

- EMBL:   L77117
- PIR:   B64418
- RefSeq:   NP_247941.1
- ProteinModelPortal:   Q58356
- GeneID:   1451843
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0946
- NMPDR:   fig|243232.1.peg.978
- TIGR:   MJ0946
- HOGENOM:   HBG577872
- OMA:   DAPVFYN
- ProtClustDB:   PRK04338
- BioCyc:   MJAN243232:MJ_0946-MONOMER
- BRENDA:   2.1.1.32
- HAMAP:   MF_00290
- InterPro:   IPR022923
- InterPro:   IPR002905
- PANTHER:   PTHR10631
- TIGRFAMs:   TIGR00308

Pfam domain/function: PF02005 TRM

EC number: =2.1.1.32

Molecular weight: Translated: 42588; Mature: 42588

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILKEGEVVFEVPDKLTVTKKDEVFYNPRMKTCRDISIAVIQAFLNLYHKRDKFYIADAL
CCCCCCCEEEECCCCEEEEECCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHH
AGSGIRGLRYAKELEFNGELKVFLNDINPKAYEKIINNAKLNEIENIDVFNEDANTFLSK
CCCCCHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHH
HFRFFNVVDIDPFGSPAPYVEQAIRALVTRNGLLCLTATDTAALCGRSKKSCLRKYLAYP
HHHEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHCCCHHHHHHHHHCCC
LFGRDCHEFALRVLVGYVMRMATKYELALKPVFCHATDHYVRVYLVTDRGAKRADKVFEM
CCCCCHHHHHHHHHHHHHHHHHHHHHHEECEEEEECCCCEEEEEEEECCCCHHHHHHHHH
LGYVKDVNGIKIIKKFEEGYEKGFAGPLYIGNLYDKALVEEALKIAEKREFSERVLKILN
HHHHHCCCHHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIKGESAINQVGCYDTHQIGKMLKISVPPMQDIINKLKEMGFNAVVTHYNPKGIKTDATL
HHCCCHHHHHCCCCCHHHHCCEEEECCCCHHHHHHHHHHCCCCEEEEEECCCCCCCHHHH
KNVIEAIYQCTKIR
HHHHHHHHHHHCCC
>Mature Secondary Structure
MILKEGEVVFEVPDKLTVTKKDEVFYNPRMKTCRDISIAVIQAFLNLYHKRDKFYIADAL
CCCCCCCEEEECCCCEEEEECCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHH
AGSGIRGLRYAKELEFNGELKVFLNDINPKAYEKIINNAKLNEIENIDVFNEDANTFLSK
CCCCCHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHH
HFRFFNVVDIDPFGSPAPYVEQAIRALVTRNGLLCLTATDTAALCGRSKKSCLRKYLAYP
HHHEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHCCCHHHHHHHHHCCC
LFGRDCHEFALRVLVGYVMRMATKYELALKPVFCHATDHYVRVYLVTDRGAKRADKVFEM
CCCCCHHHHHHHHHHHHHHHHHHHHHHEECEEEEECCCCEEEEEEEECCCCHHHHHHHHH
LGYVKDVNGIKIIKKFEEGYEKGFAGPLYIGNLYDKALVEEALKIAEKREFSERVLKILN
HHHHHCCCHHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIKGESAINQVGCYDTHQIGKMLKISVPPMQDIINKLKEMGFNAVVTHYNPKGIKTDATL
HHCCCHHHHHCCCCCHHHHCCEEEECCCCHHHHHHHHHHCCCCEEEEEECCCCCCCHHHH
KNVIEAIYQCTKIR
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087