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The map label for this gene is thiI

Identifier: 15669121

GI number: 15669121

Start: 860920

End: 862065

Strand: Direct

Name: thiI

Synonym: MJ0931

Alternate gene names: 15669121

Gene position: 860920-862065 (Clockwise)

Preceding gene: 15669120

Following gene: 15669122

Centisome position: 51.71

GC content: 29.14

Gene sequence:

>1146_bases
ATGAAAAAGCTTAAGGTGAGTAAAATGGAAATATTAGTTAGATATGGAGAAATTGGATTAAAATCAGACCCAATTAGAAA
AAACTTAGAGGAAATCTTAAGAAAAAACATTATAAAATTGCTTAGAAAATATGAGATTGATGCAGAGGTTAAAATTTTAC
ATAGGAAATTGTTAGTCAAGATAAACACAAAAGATAAAGAAGATTTAGCTCTAAAGTTATTAAAAAAAGTTGCTGGTATT
GTTTCCTACAGTCCAGTTTATGAGTGTCCATTGGATATCAACGAAATTGTAAGTTTTGCAGTTCAAATTATGAAGAAGAA
ATTAAAAACTCTAAATAAAGAAAAAGTAACTTTTGCAGTTAAAACAAAAAGGAGCTATAAAAAATTCCCATTCACATCAG
TTGAAGTAAATAAAAAAGTTGGGGAAGCTATAGTTGAAAAGCTCGGATTAGAAGTTGATTTAGAAAATCCAGATATTGTT
TTGGGAATAGAGATTTTAAATGATGGAGCATATATTTTCACAGAAAAATATGAAGGAATTGGAGGATTACCAGCAGGAAG
CCAGGGGAAAGTTCTCTGCTTAATATCTGATGGAATAGATAGCCCTGTAGCTGCATTTATGATGATTAGAAGAGGTTGTA
GAGCTGTTTTGTTACATTTAAAGATGAGTGAAGAAGCATTAAATAAAGTTAGAAAGATTGTTGAGGTTTTAAGTGACTAT
GATACCGAATTGGAGTTTGTTGTCTATGATTACAAAAAAGATATTGAGGATATTGTAGAAAAACTTAAAAGTATTAAAAA
AGAGAATTATACATGCATATTCTGTAAAAGAAAAATGCTAAAAGTCGCTGAAAAATATGCAAAATATTTAGATTGTGATG
CTATTGTTACAGGAGATAACTTGGGGCAGGTAGCTTCCCAAACATTAAAGAACTTGAGAGTCATAAGTGAAAATATAAAT
TATCCAATTTTAAGGCCTTTAATTGGTTTAGATAAGAATGATATTGTGAAAATAGCTAAAGAGATTGGAACTTATGAAAT
ATCTACTGAAAAAGAAATAAAATGTCCGTATCTTCCAAAACATCCAAAAACCATTGCTAAACCAGAAGAAGTCAAAAAAA
TAAAGGAAAAAGTCAAACTTGTATAA

Upstream 100 bases:

>100_bases
CTTAGAGAGGTAAATGTTCCAAGTATATCAACCATAGGCCCAAAAGGAGGAACTCCAGTAGCTGTTGCCATAATTAACGG
CATTATTGCCTATGCAAAAA

Downstream 100 bases:

>100_bases
AGACATTTTATAAAAATTCCTATTTCCCAATATAAGGTTTTATTTTATAAGATAAACTATCATAAACACTAAATCTAAAC
CGAAAATTTTAAATACCCTA

Product: hypothetical protein

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MKKLKVSKMEILVRYGEIGLKSDPIRKNLEEILRKNIIKLLRKYEIDAEVKILHRKLLVKINTKDKEDLALKLLKKVAGI
VSYSPVYECPLDINEIVSFAVQIMKKKLKTLNKEKVTFAVKTKRSYKKFPFTSVEVNKKVGEAIVEKLGLEVDLENPDIV
LGIEILNDGAYIFTEKYEGIGGLPAGSQGKVLCLISDGIDSPVAAFMMIRRGCRAVLLHLKMSEEALNKVRKIVEVLSDY
DTELEFVVYDYKKDIEDIVEKLKSIKKENYTCIFCKRKMLKVAEKYAKYLDCDAIVTGDNLGQVASQTLKNLRVISENIN
YPILRPLIGLDKNDIVKIAKEIGTYEISTEKEIKCPYLPKHPKTIAKPEEVKKIKEKVKLV

Sequences:

>Translated_381_residues
MKKLKVSKMEILVRYGEIGLKSDPIRKNLEEILRKNIIKLLRKYEIDAEVKILHRKLLVKINTKDKEDLALKLLKKVAGI
VSYSPVYECPLDINEIVSFAVQIMKKKLKTLNKEKVTFAVKTKRSYKKFPFTSVEVNKKVGEAIVEKLGLEVDLENPDIV
LGIEILNDGAYIFTEKYEGIGGLPAGSQGKVLCLISDGIDSPVAAFMMIRRGCRAVLLHLKMSEEALNKVRKIVEVLSDY
DTELEFVVYDYKKDIEDIVEKLKSIKKENYTCIFCKRKMLKVAEKYAKYLDCDAIVTGDNLGQVASQTLKNLRVISENIN
YPILRPLIGLDKNDIVKIAKEIGTYEISTEKEIKCPYLPKHPKTIAKPEEVKKIKEKVKLV
>Mature_381_residues
MKKLKVSKMEILVRYGEIGLKSDPIRKNLEEILRKNIIKLLRKYEIDAEVKILHRKLLVKINTKDKEDLALKLLKKVAGI
VSYSPVYECPLDINEIVSFAVQIMKKKLKTLNKEKVTFAVKTKRSYKKFPFTSVEVNKKVGEAIVEKLGLEVDLENPDIV
LGIEILNDGAYIFTEKYEGIGGLPAGSQGKVLCLISDGIDSPVAAFMMIRRGCRAVLLHLKMSEEALNKVRKIVEVLSDY
DTELEFVVYDYKKDIEDIVEKLKSIKKENYTCIFCKRKMLKVAEKYAKYLDCDAIVTGDNLGQVASQTLKNLRVISENIN
YPILRPLIGLDKNDIVKIAKEIGTYEISTEKEIKCPYLPKHPKTIAKPEEVKKIKEKVKLV

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1786625, Length=373, Percent_Identity=31.9034852546917, Blast_Score=170, Evalue=1e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THII_METJA (Q58341)

Other databases:

- EMBL:   L77117
- PIR:   C64416
- RefSeq:   NP_247926.1
- ProteinModelPortal:   Q58341
- SMR:   Q58341
- GeneID:   1451820
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0931
- NMPDR:   fig|243232.1.peg.963
- TIGR:   MJ0931
- HOGENOM:   HBG646072
- OMA:   GYPIGTQ
- ProtClustDB:   CLSK2392218
- BioCyc:   MJAN243232:MJ_0931-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00021
- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114
- Gene3D:   G3DSA:3.40.50.620
- SMART:   SM00981
- TIGRFAMs:   TIGR00342

Pfam domain/function: PF02568 ThiI; PF02926 THUMP

EC number: =2.8.1.4

Molecular weight: Translated: 43437; Mature: 43437

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: PS51165 THUMP

Important sites: BINDING 276-276 BINDING 298-298 BINDING 307-307

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLKVSKMEILVRYGEIGLKSDPIRKNLEEILRKNIIKLLRKYEIDAEVKILHRKLLVK
CCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEE
INTKDKEDLALKLLKKVAGIVSYSPVYECPLDINEIVSFAVQIMKKKLKTLNKEKVTFAV
ECCCCHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCHHHEEEEE
KTKRSYKKFPFTSVEVNKKVGEAIVEKLGLEVDLENPDIVLGIEILNDGAYIFTEKYEGI
ECCCCHHCCCCCEEEHHHHHHHHHHHHHCCEEECCCCCEEEEEEEECCCEEEEEECCCCC
GGLPAGSQGKVLCLISDGIDSPVAAFMMIRRGCRAVLLHLKMSEEALNKVRKIVEVLSDY
CCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
DTELEFVVYDYKKDIEDIVEKLKSIKKENYTCIFCKRKMLKVAEKYAKYLDCDAIVTGDN
CCCEEEEEEHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHCCCCEEEECCC
LGQVASQTLKNLRVISENINYPILRPLIGLDKNDIVKIAKEIGTYEISTEKEIKCPYLPK
HHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHCCEEECCCCCCCCCCCCC
HPKTIAKPEEVKKIKEKVKLV
CCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKLKVSKMEILVRYGEIGLKSDPIRKNLEEILRKNIIKLLRKYEIDAEVKILHRKLLVK
CCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEE
INTKDKEDLALKLLKKVAGIVSYSPVYECPLDINEIVSFAVQIMKKKLKTLNKEKVTFAV
ECCCCHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCHHHEEEEE
KTKRSYKKFPFTSVEVNKKVGEAIVEKLGLEVDLENPDIVLGIEILNDGAYIFTEKYEGI
ECCCCHHCCCCCEEEHHHHHHHHHHHHHCCEEECCCCCEEEEEEEECCCEEEEEECCCCC
GGLPAGSQGKVLCLISDGIDSPVAAFMMIRRGCRAVLLHLKMSEEALNKVRKIVEVLSDY
CCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
DTELEFVVYDYKKDIEDIVEKLKSIKKENYTCIFCKRKMLKVAEKYAKYLDCDAIVTGDN
CCCEEEEEEHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHCCCCEEEECCC
LGQVASQTLKNLRVISENINYPILRPLIGLDKNDIVKIAKEIGTYEISTEKEIKCPYLPK
HHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHCCEEECCCCCCCCCCCCC
HPKTIAKPEEVKKIKEKVKLV
CCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087