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The map label for this gene is purB

Identifier: 15669119

GI number: 15669119

Start: 858840

End: 860228

Strand: Direct

Name: purB

Synonym: MJ0929

Alternate gene names: 15669119

Gene position: 858840-860228 (Clockwise)

Preceding gene: 15669116

Following gene: 15669120

Centisome position: 51.58

GC content: 34.92

Gene sequence:

>1389_bases
TTGGAACTTAATCAAATAATAAAGTCAGGTGAAAAAATGGCTGTGCATCCAATTGATTATAGATATGGAACACCAGAGAT
GAGAAAAGTTTGGGAAGAAGAAAATAAATTGGAAAAAATGTTAAAGGTAGAGGCTGCATTAGCTAAAGCTCAGGCAGAAC
TCGGCTTAATTCCAAAAGAAGCCGCTGAAGAGATAAACAAAAAAGCATCAACAAAATATGTAAAATTGGAGAGAGTTAAA
GAAATTGAGAAACAAACAAAACATGATGTTGTTGCAATGATTAGAGCTTTAGCTGAAGTATGTGAAGGAAATGCTGGAGA
ATACATACACTTCGGAGCTACATCAAACGATATTGTTGATACTGCCAACTCCCTACTAATAAAAGAATCCATTGAAATTA
TAGAAGATAAGTTAAAGCAGTTAAGAGATATATTATTAGACAAAGCAGAAGAGCATAAATACACTGTCTGTGTAGGAAGA
ACACATGGACAGCATGCAATTCCAACAACCTATGGGATGAGATTTGCTCTATGGGCGGCTGAGATTGACAGACACTTAGA
GAGATTAAAAGAAGCAAAAAAGAGAATATGCGTCTCTATGATTACTGGAGCTGTTGGAACAATGGCGGCTATGGGAGAGA
AGGGTTTGGAAGTGCATAAAAGAGTTGCTGAAATCTTAGGCTTAGAACCAGTTTTAATCTCAAACCAAGTTATTCAGAGA
GACAGACATGCTGAATTCGTCTTTTTATTAGCTTTAATTGCTCAGACATTAAACAAGATTGGAGTTACTGTTAGAAGTAT
GCAGAGAACTGAAATTGGAGAGCTAGAAGAAGAGTTCGACCCTACAAAGCAAACTGGTTCATCAACAATGCCTCACAAGA
GAAATCCAATAACCTTTGAGCAAATCTGCGGTTTATCAAGGGTTATAAAATCACTATGTATAGCTGAAATGGACAATATC
CCATTATGGGAAGAGAGGGATTTAACAAACTCATCAGCTGAGAGATGTATATTTGCAGAGGTTTGCGTTTTAACAGACCA
CATCTTAACATTAGCAATTAAAGGAGTTAAAAAGCTAAAAGTAAATAAAGAAAATGTTGAGAGAAACTTAGAATTAACAA
AAGGACTGATAATGGCTGAGAGAATAATGATTGAATTGGCTAAGAGAGGTATGGGCAGACAAACAGCTCATGAAATAGTA
AGGCAGTGTGCAATGAAAGCCTATGAAGAGAAAAGGCATTTAAAAGATATTTTATTAGAAAATGAGGAAGTTATGAAGTA
TATAACAAAAGAAGAGTTAGAGGAATTGATGAATCCTAAAACTTACATAGGTTTAGCTCCACAGATAGTTGATGAAGTTA
TTAAAACTCTAAAGAATAAAAAGTACTAA

Upstream 100 bases:

>100_bases
CTAAAACCCAAATAATAAATTATAAACAGACTTCTATAAGTAATTTTCTGAAAATGCTTTTTTAAATAAACATCAAACTA
TAAAAATCATTAGCAATTAA

Downstream 100 bases:

>100_bases
AAATAATGGCTTATCCTATTTTTTCTATTTTTAACTTCTATATTTTCAGTTTTGGTGATTTTTATGGATTTTATGGGAGC
AATATCAAAAGATGGATTAA

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MELNQIIKSGEKMAVHPIDYRYGTPEMRKVWEEENKLEKMLKVEAALAKAQAELGLIPKEAAEEINKKASTKYVKLERVK
EIEKQTKHDVVAMIRALAEVCEGNAGEYIHFGATSNDIVDTANSLLIKESIEIIEDKLKQLRDILLDKAEEHKYTVCVGR
THGQHAIPTTYGMRFALWAAEIDRHLERLKEAKKRICVSMITGAVGTMAAMGEKGLEVHKRVAEILGLEPVLISNQVIQR
DRHAEFVFLLALIAQTLNKIGVTVRSMQRTEIGELEEEFDPTKQTGSSTMPHKRNPITFEQICGLSRVIKSLCIAEMDNI
PLWEERDLTNSSAERCIFAEVCVLTDHILTLAIKGVKKLKVNKENVERNLELTKGLIMAERIMIELAKRGMGRQTAHEIV
RQCAMKAYEEKRHLKDILLENEEVMKYITKEELEELMNPKTYIGLAPQIVDEVIKTLKNKKY

Sequences:

>Translated_462_residues
MELNQIIKSGEKMAVHPIDYRYGTPEMRKVWEEENKLEKMLKVEAALAKAQAELGLIPKEAAEEINKKASTKYVKLERVK
EIEKQTKHDVVAMIRALAEVCEGNAGEYIHFGATSNDIVDTANSLLIKESIEIIEDKLKQLRDILLDKAEEHKYTVCVGR
THGQHAIPTTYGMRFALWAAEIDRHLERLKEAKKRICVSMITGAVGTMAAMGEKGLEVHKRVAEILGLEPVLISNQVIQR
DRHAEFVFLLALIAQTLNKIGVTVRSMQRTEIGELEEEFDPTKQTGSSTMPHKRNPITFEQICGLSRVIKSLCIAEMDNI
PLWEERDLTNSSAERCIFAEVCVLTDHILTLAIKGVKKLKVNKENVERNLELTKGLIMAERIMIELAKRGMGRQTAHEIV
RQCAMKAYEEKRHLKDILLENEEVMKYITKEELEELMNPKTYIGLAPQIVDEVIKTLKNKKY
>Mature_462_residues
MELNQIIKSGEKMAVHPIDYRYGTPEMRKVWEEENKLEKMLKVEAALAKAQAELGLIPKEAAEEINKKASTKYVKLERVK
EIEKQTKHDVVAMIRALAEVCEGNAGEYIHFGATSNDIVDTANSLLIKESIEIIEDKLKQLRDILLDKAEEHKYTVCVGR
THGQHAIPTTYGMRFALWAAEIDRHLERLKEAKKRICVSMITGAVGTMAAMGEKGLEVHKRVAEILGLEPVLISNQVIQR
DRHAEFVFLLALIAQTLNKIGVTVRSMQRTEIGELEEEFDPTKQTGSSTMPHKRNPITFEQICGLSRVIKSLCIAEMDNI
PLWEERDLTNSSAERCIFAEVCVLTDHILTLAIKGVKKLKVNKENVERNLELTKGLIMAERIMIELAKRGMGRQTAHEIV
RQCAMKAYEEKRHLKDILLENEEVMKYITKEELEELMNPKTYIGLAPQIVDEVIKTLKNKKY

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily

Homologues:

Organism=Homo sapiens, GI4557269, Length=453, Percent_Identity=28.2560706401766, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI183227688, Length=388, Percent_Identity=29.1237113402062, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI31541964, Length=315, Percent_Identity=26.031746031746, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI68303542, Length=315, Percent_Identity=26.031746031746, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI19743875, Length=357, Percent_Identity=24.6498599439776, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1787376, Length=479, Percent_Identity=30.062630480167, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1790398, Length=367, Percent_Identity=22.6158038147139, Blast_Score=74, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17508577, Length=393, Percent_Identity=30.2798982188295, Blast_Score=148, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI32564234, Length=350, Percent_Identity=30.5714285714286, Blast_Score=125, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17553882, Length=351, Percent_Identity=25.9259259259259, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI32565146, Length=344, Percent_Identity=26.453488372093, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323391, Length=455, Percent_Identity=29.010989010989, Blast_Score=172, Evalue=7e-44,
Organism=Saccharomyces cerevisiae, GI6321806, Length=400, Percent_Identity=24, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24647570, Length=460, Percent_Identity=28.695652173913, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI78710009, Length=360, Percent_Identity=29.1666666666667, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24662684, Length=470, Percent_Identity=24.6808510638298, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24640179, Length=351, Percent_Identity=25.3561253561254, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24640177, Length=351, Percent_Identity=25.3561253561254, Blast_Score=80, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PUR8_METJA (Q58339)

Other databases:

- EMBL:   L77117
- PIR:   A64416
- RefSeq:   NP_247924.1
- ProteinModelPortal:   Q58339
- SMR:   Q58339
- GeneID:   1451818
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0929
- NMPDR:   fig|243232.1.peg.961
- TIGR:   MJ0929
- HOGENOM:   HBG499008
- OMA:   HAVPTTY
- ProtClustDB:   PRK08540
- BioCyc:   MJAN243232:MJ_0929-MONOMER
- BRENDA:   4.3.2.2
- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769
- PRINTS:   PR00145
- PRINTS:   PR00149
- TIGRFAMs:   TIGR00928

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1; SSF48557 L-Aspartase-like

EC number: =4.3.2.2

Molecular weight: Translated: 52593; Mature: 52593

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: ACT_SITE 88-88 ACT_SITE 162-162

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELNQIIKSGEKMAVHPIDYRYGTPEMRKVWEEENKLEKMLKVEAALAKAQAELGLIPKE
CCHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
AAEEINKKASTKYVKLERVKEIEKQTKHDVVAMIRALAEVCEGNAGEYIHFGATSNDIVD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHH
TANSLLIKESIEIIEDKLKQLRDILLDKAEEHKYTVCVGRTHGQHAIPTTYGMRFALWAA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHH
EIDRHLERLKEAKKRICVSMITGAVGTMAAMGEKGLEVHKRVAEILGLEPVLISNQVIQR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHHHHHH
DRHAEFVFLLALIAQTLNKIGVTVRSMQRTEIGELEEEFDPTKQTGSSTMPHKRNPITFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCHH
QICGLSRVIKSLCIAEMDNIPLWEERDLTNSSAERCIFAEVCVLTDHILTLAIKGVKKLK
HHHCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VNKENVERNLELTKGLIMAERIMIELAKRGMGRQTAHEIVRQCAMKAYEEKRHLKDILLE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NEEVMKYITKEELEELMNPKTYIGLAPQIVDEVIKTLKNKKY
CHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MELNQIIKSGEKMAVHPIDYRYGTPEMRKVWEEENKLEKMLKVEAALAKAQAELGLIPKE
CCHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
AAEEINKKASTKYVKLERVKEIEKQTKHDVVAMIRALAEVCEGNAGEYIHFGATSNDIVD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHH
TANSLLIKESIEIIEDKLKQLRDILLDKAEEHKYTVCVGRTHGQHAIPTTYGMRFALWAA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHH
EIDRHLERLKEAKKRICVSMITGAVGTMAAMGEKGLEVHKRVAEILGLEPVLISNQVIQR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHHHHHH
DRHAEFVFLLALIAQTLNKIGVTVRSMQRTEIGELEEEFDPTKQTGSSTMPHKRNPITFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCHH
QICGLSRVIKSLCIAEMDNIPLWEERDLTNSSAERCIFAEVCVLTDHILTLAIKGVKKLK
HHHCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VNKENVERNLELTKGLIMAERIMIELAKRGMGRQTAHEIVRQCAMKAYEEKRHLKDILLE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NEEVMKYITKEELEELMNPKTYIGLAPQIVDEVIKTLKNKKY
CHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087