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The map label for this gene is nirB [C]

Identifier: 15669061

GI number: 15669061

Start: 792985

End: 794847

Strand: Reverse

Name: nirB [C]

Synonym: MJ0870

Alternate gene names: 15669061

Gene position: 794847-792985 (Counterclockwise)

Preceding gene: 15669063

Following gene: 15669059

Centisome position: 47.74

GC content: 35.37

Gene sequence:

>1863_bases
ATGTATGAGTGGAAGTTAAATGAAATAGTCGATAGTGGAGTATGTGCAAGATGTGGGACCTGCACTATAGTATGTCCTAA
TGGTATATTAACCTTTGATGAAAGACCAAAGTTAATCGATGAATGTTTAAGAAAAGGTCATGGAATGTGTTTTGAAGTAT
GTCCAAGAGTTTCTTCTGCAAAGTATCAGATAAAGATTAGAGAGAAGTTTTATGAAAAATACTATTATGCAAAAAGTGAT
ATTGAAGGACAAGATGGGGGAGTTGTTACAGCATTTCTAAAATACCTATTAGAAAACGGAAAGATAGATGGAGCTATAGT
CGTTGGAGATGAATGCTGGAAACCAGTTTCATTGGTTGTTCAAAATGCAGAGGATTTATTAAAAACTGCAAAATCAAAAT
ATGCAATCTCAACCTTAGATGCATTAAGAAAGGCTGGAGAGATGGGTTTAGAGAAAGTTGCTGTTGTTGGATTGCCTTGC
CAAATTAACGGATTGAGAAAACTGCAGTATTTCCCATACCATGCTAAGCACGACCTTGAATTAGGAAGAAATGGAAAGCC
AGTAAAACTGCCAAAAATAGAGTATTTAATTGGCTTATTCTGCACTGAGAAGTTTAGATACGACAACATGAAGGAAGTTC
TATCAAAACATGGAATAGATATTGAAAAAGTTGAGAAATTTGACATTAAGAAAGGAAAACTCCTCGTTTATGTAAATGGA
GAGAAGAAGGAATTTGACCTAAAAGAGTTTGAAATCTGCTCTGGCTGTAAGATGTGTAGGGATTTTGATGCAGAGATGGC
GGATGTTTCAGTTGGGTGTGTTGGAAGTCCAGATGGTTATTCAACAATCATAATAAGAACTGAAAAGGGAGAGGAAATTA
AAAATGCTGTAGAATTAAAAGAAGGAGTTAATTTAGAAGAAATTGAGAAATTAAGACAGCTAAAATTAAAGAGATTTAAG
AAAGAAGTTGAGAGAAGGAGAGAAAATAATGAGTATGTTTCATTCTACTGGACTGCAGATTACGGAGGAATTGGAAAGAG
AGCAGATGGAACATACTTTATAAGAGTTAGAGCTAAGCCAGGAGGATGGTATAAGCCAGAGGAGATAAAAGAAATTTTAG
ATATTGCAGAAGAATACAATGCAAAGATAAAAGTAACTGATAGAGCTGGCTATGAACTTCACGGTATTAGTGGATTTGAT
GTTGAAGATATTGTTTTAAGGTTGAGAGAAAAAGGTCTTCTAACAGGTTCAGAGGGGCCTTTAGTCAGAGCAACATTGGC
TTGTCCTGGAGGAGGAAACTGTAGCAGTGGTTTAGTAGATACAACAGAACTTGCAAGAATCATTGAAGATAACTTCAAAG
AGAGACCTGCTCCATATAAGTTTAAAATTGCAATTAGCGGTTGCCCAAACGGATGTGTAAGACCACAAGTTCATGATATT
GGAATAGCTGGAGTAAAATATCCAAAGGTAAATGAAGAAAAATGTAACGGTTGCGGAAGATGTGCTGAGGTTTGTAAGGT
TGAGGCAATTGATATTAGAGGAGAAACATCTTACACAAATTACAACGTATGTGTTGGCTGCGGAAAATGTATTAAAAACT
GTCCAAATGAGGCAAGGGAAGTTAAAGAAGAGGGTTATTTAGTTTATGTTGGTGGAAAAACTGGAAGAGAGGTTGTTGAA
GGAGTTAAAATGAAGTTGATGAGTGTTGATGAAATTATAAACTTTATTGATAAGGTGTTGGTTGTTTATGGCAAATATGC
TGAAAAACCACAAAGAGAAAGATTAGCTGCAGTTATGAAAAGAGTTGGGTATGGAAAGTTCTTAGAAGAAGTAAAAGAGT
TGATGAAAAAAGAAATCTGCTAA

Upstream 100 bases:

>100_bases
TATATGTGTGTAATTCATGAACACATTAGTAAGTTTTATATAGTTTTATTAAATAGCATTAAAGTGTGAATAAATCAATC
ACACATTTAGTGGTGAAAAA

Downstream 100 bases:

>100_bases
TTAATTTTTTAGATGTTTTATTTCTTTTATTTTTTAAAGATATATTTTAAAAATTAATTTAAAGAATTAATCATGAATTC
CCTTTTCGGTTATCCTAAAC

Product: coenzyme F420-reducing hydrogenase subunit beta

Products: 3 NAD(P)+; NH4OH; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 620; Mature: 620

Protein sequence:

>620_residues
MYEWKLNEIVDSGVCARCGTCTIVCPNGILTFDERPKLIDECLRKGHGMCFEVCPRVSSAKYQIKIREKFYEKYYYAKSD
IEGQDGGVVTAFLKYLLENGKIDGAIVVGDECWKPVSLVVQNAEDLLKTAKSKYAISTLDALRKAGEMGLEKVAVVGLPC
QINGLRKLQYFPYHAKHDLELGRNGKPVKLPKIEYLIGLFCTEKFRYDNMKEVLSKHGIDIEKVEKFDIKKGKLLVYVNG
EKKEFDLKEFEICSGCKMCRDFDAEMADVSVGCVGSPDGYSTIIIRTEKGEEIKNAVELKEGVNLEEIEKLRQLKLKRFK
KEVERRRENNEYVSFYWTADYGGIGKRADGTYFIRVRAKPGGWYKPEEIKEILDIAEEYNAKIKVTDRAGYELHGISGFD
VEDIVLRLREKGLLTGSEGPLVRATLACPGGGNCSSGLVDTTELARIIEDNFKERPAPYKFKIAISGCPNGCVRPQVHDI
GIAGVKYPKVNEEKCNGCGRCAEVCKVEAIDIRGETSYTNYNVCVGCGKCIKNCPNEAREVKEEGYLVYVGGKTGREVVE
GVKMKLMSVDEIINFIDKVLVVYGKYAEKPQRERLAAVMKRVGYGKFLEEVKELMKKEIC

Sequences:

>Translated_620_residues
MYEWKLNEIVDSGVCARCGTCTIVCPNGILTFDERPKLIDECLRKGHGMCFEVCPRVSSAKYQIKIREKFYEKYYYAKSD
IEGQDGGVVTAFLKYLLENGKIDGAIVVGDECWKPVSLVVQNAEDLLKTAKSKYAISTLDALRKAGEMGLEKVAVVGLPC
QINGLRKLQYFPYHAKHDLELGRNGKPVKLPKIEYLIGLFCTEKFRYDNMKEVLSKHGIDIEKVEKFDIKKGKLLVYVNG
EKKEFDLKEFEICSGCKMCRDFDAEMADVSVGCVGSPDGYSTIIIRTEKGEEIKNAVELKEGVNLEEIEKLRQLKLKRFK
KEVERRRENNEYVSFYWTADYGGIGKRADGTYFIRVRAKPGGWYKPEEIKEILDIAEEYNAKIKVTDRAGYELHGISGFD
VEDIVLRLREKGLLTGSEGPLVRATLACPGGGNCSSGLVDTTELARIIEDNFKERPAPYKFKIAISGCPNGCVRPQVHDI
GIAGVKYPKVNEEKCNGCGRCAEVCKVEAIDIRGETSYTNYNVCVGCGKCIKNCPNEAREVKEEGYLVYVGGKTGREVVE
GVKMKLMSVDEIINFIDKVLVVYGKYAEKPQRERLAAVMKRVGYGKFLEEVKELMKKEIC
>Mature_620_residues
MYEWKLNEIVDSGVCARCGTCTIVCPNGILTFDERPKLIDECLRKGHGMCFEVCPRVSSAKYQIKIREKFYEKYYYAKSD
IEGQDGGVVTAFLKYLLENGKIDGAIVVGDECWKPVSLVVQNAEDLLKTAKSKYAISTLDALRKAGEMGLEKVAVVGLPC
QINGLRKLQYFPYHAKHDLELGRNGKPVKLPKIEYLIGLFCTEKFRYDNMKEVLSKHGIDIEKVEKFDIKKGKLLVYVNG
EKKEFDLKEFEICSGCKMCRDFDAEMADVSVGCVGSPDGYSTIIIRTEKGEEIKNAVELKEGVNLEEIEKLRQLKLKRFK
KEVERRRENNEYVSFYWTADYGGIGKRADGTYFIRVRAKPGGWYKPEEIKEILDIAEEYNAKIKVTDRAGYELHGISGFD
VEDIVLRLREKGLLTGSEGPLVRATLACPGGGNCSSGLVDTTELARIIEDNFKERPAPYKFKIAISGCPNGCVRPQVHDI
GIAGVKYPKVNEEKCNGCGRCAEVCKVEAIDIRGETSYTNYNVCVGCGKCIKNCPNEAREVKEEGYLVYVGGKTGREVVE
GVKMKLMSVDEIINFIDKVLVVYGKYAEKPQRERLAAVMKRVGYGKFLEEVKELMKKEIC

Specific function: Nitrate assimilation (denitrification); second step. [C]

COG id: COG1035

COG function: function code C; Coenzyme F420-reducing hydrogenase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Escherichia coli, GI1789765, Length=135, Percent_Identity=32.5925925925926, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y870_METJA (Q58280)

Other databases:

- EMBL:   L77117
- PIR:   F64408
- RefSeq:   NP_247865.1
- ProteinModelPortal:   Q58280
- GeneID:   1451759
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0870
- NMPDR:   fig|243232.1.peg.902
- TIGR:   MJ0870
- HOGENOM:   HBG539702
- OMA:   VRATLAC
- ProtClustDB:   CLSK796624
- BioCyc:   MJAN243232:MJ_0870-MONOMER
- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR007516
- InterPro:   IPR007525
- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066
- PRINTS:   PR00397

Pfam domain/function: PF00037 Fer4; PF04432 FrhB_FdhB_C; PF04422 FrhB_FdhB_N; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; SSF55124 NiR_SiRalpha_1/3

EC number: 1.7.1.4 [C]

Molecular weight: Translated: 69795; Mature: 69795

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2; PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
4.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYEWKLNEIVDSGVCARCGTCTIVCPNGILTFDERPKLIDECLRKGHGMCFEVCPRVSSA
CCCCCHHHHHCCCCCCCCCEEEEECCCCEEECCCCHHHHHHHHHHCCCCCHHHCCCCCCC
KYQIKIREKFYEKYYYAKSDIEGQDGGVVTAFLKYLLENGKIDGAIVVGDECWKPVSLVV
EEEEEHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHH
QNAEDLLKTAKSKYAISTLDALRKAGEMGLEKVAVVGLPCQINGLRKLQYFPYHAKHDLE
HCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEECCCEEEEECCCCCCCCCC
LGRNGKPVKLPKIEYLIGLFCTEKFRYDNMKEVLSKHGIDIEKVEKFDIKKGKLLVYVNG
CCCCCCCEECCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHCCCCCCEEEEEECC
EKKEFDLKEFEICSGCKMCRDFDAEMADVSVGCVGSPDGYSTIIIRTEKGEEIKNAVELK
CCCCCCCHHHHHHHHHHHHHHCCCCHHCEEEEECCCCCCCEEEEEEECCCHHHHHHHHHH
EGVNLEEIEKLRQLKLKRFKKEVERRRENNEYVSFYWTADYGGIGKRADGTYFIRVRAKP
HCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCEEEEEEEECC
GGWYKPEEIKEILDIAEEYNAKIKVTDRAGYELHGISGFDVEDIVLRLREKGLLTGSEGP
CCCCCHHHHHHHHHHHHHCCCEEEEEECCCEEEECCCCCCHHHHHHHHHHCCCEECCCCC
LVRATLACPGGGNCSSGLVDTTELARIIEDNFKERPAPYKFKIAISGCPNGCVRPQVHDI
EEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCC
GIAGVKYPKVNEEKCNGCGRCAEVCKVEAIDIRGETSYTNYNVCVGCGKCIKNCPNEARE
CCCCCCCCCCCHHHCCCHHHHHHHHEEEEEEECCCCCCCCCEEEEEHHHHHHHCCHHHHH
VKEEGYLVYVGGKTGREVVEGVKMKLMSVDEIINFIDKVLVVYGKYAEKPQRERLAAVMK
HHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
RVGYGKFLEEVKELMKKEIC
HCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MYEWKLNEIVDSGVCARCGTCTIVCPNGILTFDERPKLIDECLRKGHGMCFEVCPRVSSA
CCCCCHHHHHCCCCCCCCCEEEEECCCCEEECCCCHHHHHHHHHHCCCCCHHHCCCCCCC
KYQIKIREKFYEKYYYAKSDIEGQDGGVVTAFLKYLLENGKIDGAIVVGDECWKPVSLVV
EEEEEHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHH
QNAEDLLKTAKSKYAISTLDALRKAGEMGLEKVAVVGLPCQINGLRKLQYFPYHAKHDLE
HCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEECCCEEEEECCCCCCCCCC
LGRNGKPVKLPKIEYLIGLFCTEKFRYDNMKEVLSKHGIDIEKVEKFDIKKGKLLVYVNG
CCCCCCCEECCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHCCCCCCEEEEEECC
EKKEFDLKEFEICSGCKMCRDFDAEMADVSVGCVGSPDGYSTIIIRTEKGEEIKNAVELK
CCCCCCCHHHHHHHHHHHHHHCCCCHHCEEEEECCCCCCCEEEEEEECCCHHHHHHHHHH
EGVNLEEIEKLRQLKLKRFKKEVERRRENNEYVSFYWTADYGGIGKRADGTYFIRVRAKP
HCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCEEEEEEEECC
GGWYKPEEIKEILDIAEEYNAKIKVTDRAGYELHGISGFDVEDIVLRLREKGLLTGSEGP
CCCCCHHHHHHHHHHHHHCCCEEEEEECCCEEEECCCCCCHHHHHHHHHHCCCEECCCCC
LVRATLACPGGGNCSSGLVDTTELARIIEDNFKERPAPYKFKIAISGCPNGCVRPQVHDI
EEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCC
GIAGVKYPKVNEEKCNGCGRCAEVCKVEAIDIRGETSYTNYNVCVGCGKCIKNCPNEARE
CCCCCCCCCCCHHHCCCHHHHHHHHEEEEEEECCCCCCCCCEEEEEHHHHHHHCCHHHHH
VKEEGYLVYVGGKTGREVVEGVKMKLMSVDEIINFIDKVLVVYGKYAEKPQRERLAAVMK
HHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
RVGYGKFLEEVKELMKKEIC
HCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD; NAD+; NADH; Siroheme [C]

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.4 {NO2-}} 0.005 {NO2-}} 0.016 {NADH}} 5.3 {hydroxylamine}} [C]

Substrates: 3 NAD(P)H; nitrite [C]

Specific reaction: 3 NAD(P)H + nitrite = 3 NAD(P)+ + NH4OH + H2O [C]

General reaction: Redox reaction [C]

Inhibitor: 1, 10-Phenanthroline; 2, 2'-Bipyridine; Arsenite; CN-; NAD+; NADP+; NaN3; p-Chloromercuribenzoate; SO32- [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087