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The map label for this gene is priA

Identifier: 15669029

GI number: 15669029

Start: 764853

End: 765905

Strand: Reverse

Name: priA

Synonym: MJ0839

Alternate gene names: 15669029

Gene position: 765905-764853 (Counterclockwise)

Preceding gene: 15669031

Following gene: 15669026

Centisome position: 46.0

GC content: 26.12

Gene sequence:

>1053_bases
ATGAATACTTTTGCTGAGGTTCAAAAATTGTATAGGGAATATTACAACTTCGCAATAAAAAACAATATCCTTGAAATCCC
TGAAGGTATTGAATATAGGGAATTTGGATATGGTTATTTAAAAAAAGTTGATAATAGAAACCTATCTTTTAAAAATGAAA
GAGAATATAAAGATTGGGTGTTAAAAAACGCTCCAATGCACTTTTATAAATCTTTAGCTTATATGCTCTACCCAAATAAA
TCAGGTGGAGCTTCTAAAAAAGGTATATTTAGAAGAGAATTAGCGTTTGATATAGATGTCCATAAAACAAAAAAATGTAA
GCATGAAGATGATTGGATTTGCAAGCATTGTTTAGAAGAGGCAAAAAATCAAGCTATCTACTTAATTGAAGAATTTTTAA
TTCCTGACTTTGGTTTAAATGAAGAAGATTTAAAGATTGTATTTAGTGGAAACAGGGGTTATCACATATATATAAAACCA
AGAGATGAAAAAATTAGGGATATTATTGAAAGCTATTCAAAAGAAGATAGAAGATTTTTAATGGATTATATATTAGGAAA
AAATTTAAACTTAAATTCAGTAGGTAGTGGTTGGAGAAGAAGATTAATAAAGGCAATCAAAGAAAGAGATAAAAGAATAT
CCACAAAAAAACTTGAAAATGAAAAAAATTGGAAAAAGGTTATTGAGAATTTAAAGAGCAAAAATAAAATATATAATATT
ATTGAAGAAACCAAAAATAAAATTGAATTGGATGAAAAAGTTATGGATGATGACATAAGGCTTTTGAGGGTTATAAATTC
TCTACATGGCTATACTGGCTTTATTGTTAAGCCATTAAGTGGTTTAGATGAATTAAGGAGATTTAACCCATTAGAAGATG
CCATATTTAAAGATTTTGAAAATAAAGTGTATGAGGTTAATATATTTGATGATAGAAAATTCGAAATTGAAATATGTGGA
AAGAAATACAACAATAAAAGTAAAAAAATTACTGCTTCAGCTTTACTATATTTATTTGGTCATAATATTAAATTTGAATT
ACTTAAATCCTAA

Upstream 100 bases:

>100_bases
TATTCCCAAAATCATAATTTTCACCGTGAAAAATTTTATAACTCTCTAAAATAATTATTGTTAATTCCTAAGTCAATACT
AAAAGTTGAGGGATAAGTAT

Downstream 100 bases:

>100_bases
GGACTTAACGCACTATCAAATATGGAATTTGAATTTATGGAATTTGAATTACTTGGATACCACTAACTAATATTTTACCA
GCTTTTGTTTTCTTTCCTTC

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MNTFAEVQKLYREYYNFAIKNNILEIPEGIEYREFGYGYLKKVDNRNLSFKNEREYKDWVLKNAPMHFYKSLAYMLYPNK
SGGASKKGIFRRELAFDIDVHKTKKCKHEDDWICKHCLEEAKNQAIYLIEEFLIPDFGLNEEDLKIVFSGNRGYHIYIKP
RDEKIRDIIESYSKEDRRFLMDYILGKNLNLNSVGSGWRRRLIKAIKERDKRISTKKLENEKNWKKVIENLKSKNKIYNI
IEETKNKIELDEKVMDDDIRLLRVINSLHGYTGFIVKPLSGLDELRRFNPLEDAIFKDFENKVYEVNIFDDRKFEIEICG
KKYNNKSKKITASALLYLFGHNIKFELLKS

Sequences:

>Translated_350_residues
MNTFAEVQKLYREYYNFAIKNNILEIPEGIEYREFGYGYLKKVDNRNLSFKNEREYKDWVLKNAPMHFYKSLAYMLYPNK
SGGASKKGIFRRELAFDIDVHKTKKCKHEDDWICKHCLEEAKNQAIYLIEEFLIPDFGLNEEDLKIVFSGNRGYHIYIKP
RDEKIRDIIESYSKEDRRFLMDYILGKNLNLNSVGSGWRRRLIKAIKERDKRISTKKLENEKNWKKVIENLKSKNKIYNI
IEETKNKIELDEKVMDDDIRLLRVINSLHGYTGFIVKPLSGLDELRRFNPLEDAIFKDFENKVYEVNIFDDRKFEIEICG
KKYNNKSKKITASALLYLFGHNIKFELLKS
>Mature_350_residues
MNTFAEVQKLYREYYNFAIKNNILEIPEGIEYREFGYGYLKKVDNRNLSFKNEREYKDWVLKNAPMHFYKSLAYMLYPNK
SGGASKKGIFRRELAFDIDVHKTKKCKHEDDWICKHCLEEAKNQAIYLIEEFLIPDFGLNEEDLKIVFSGNRGYHIYIKP
RDEKIRDIIESYSKEDRRFLMDYILGKNLNLNSVGSGWRRRLIKAIKERDKRISTKKLENEKNWKKVIENLKSKNKIYNI
IEETKNKIELDEKVMDDDIRLLRVINSLHGYTGFIVKPLSGLDELRRFNPLEDAIFKDFENKVYEVNIFDDRKFEIEICG
KKYNNKSKKITASALLYLFGHNIKFELLKS

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis

COG id: COG1467

COG function: function code L; Eukaryotic-type DNA primase, catalytic (small) subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eukaryotic-type primase small subunit family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PRIS_METJA (Q58249)

Other databases:

- EMBL:   L77117
- RefSeq:   NP_247833.1
- ProteinModelPortal:   Q58249
- GeneID:   1451727
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0839
- NMPDR:   fig|243232.1.peg.870
- TIGR:   MJ0839
- HOGENOM:   HBG541250
- OMA:   SLHGYTG
- ProtClustDB:   CLSK876359
- BioCyc:   MJAN243232:MJ_0839-MONOMER
- HAMAP:   MF_00700
- InterPro:   IPR002755
- InterPro:   IPR014052
- TIGRFAMs:   TIGR00335

Pfam domain/function: PF01896 DNA_primase_S

EC number: 2.7.7.-

Molecular weight: Translated: 41803; Mature: 41803

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: NA

Important sites: ACT_SITE 97-97 ACT_SITE 99-99

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTFAEVQKLYREYYNFAIKNNILEIPEGIEYREFGYGYLKKVDNRNLSFKNEREYKDWV
CCCHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHCCCHHHHHHCCCCCCCCCCHHHHHHH
LKNAPMHFYKSLAYMLYPNKSGGASKKGIFRRELAFDIDVHKTKKCKHEDDWICKHCLEE
HCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHEECCCHHCCCCCCCCHHHHHHHHHH
AKNQAIYLIEEFLIPDFGLNEEDLKIVFSGNRGYHIYIKPRDEKIRDIIESYSKEDRRFL
HCCCEEEHHHHHHCCCCCCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHCHHHHHHH
MDYILGKNLNLNSVGSGWRRRLIKAIKERDKRISTKKLENEKNWKKVIENLKSKNKIYNI
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
IEETKNKIELDEKVMDDDIRLLRVINSLHGYTGFIVKPLSGLDELRRFNPLEDAIFKDFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHCCCHHHHHHHHHC
NKVYEVNIFDDRKFEIEICGKKYNNKSKKITASALLYLFGHNIKFELLKS
CCEEEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEECC
>Mature Secondary Structure
MNTFAEVQKLYREYYNFAIKNNILEIPEGIEYREFGYGYLKKVDNRNLSFKNEREYKDWV
CCCHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHCCCHHHHHHCCCCCCCCCCHHHHHHH
LKNAPMHFYKSLAYMLYPNKSGGASKKGIFRRELAFDIDVHKTKKCKHEDDWICKHCLEE
HCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHEECCCHHCCCCCCCCHHHHHHHHHH
AKNQAIYLIEEFLIPDFGLNEEDLKIVFSGNRGYHIYIKPRDEKIRDIIESYSKEDRRFL
HCCCEEEHHHHHHCCCCCCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHCHHHHHHH
MDYILGKNLNLNSVGSGWRRRLIKAIKERDKRISTKKLENEKNWKKVIENLKSKNKIYNI
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
IEETKNKIELDEKVMDDDIRLLRVINSLHGYTGFIVKPLSGLDELRRFNPLEDAIFKDFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHCCCHHHHHHHHHC
NKVYEVNIFDDRKFEIEICGKKYNNKSKKITASALLYLFGHNIKFELLKS
CCEEEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 10536154