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The map label for this gene is bioB [C]

Identifier: 15668967

GI number: 15668967

Start: 708313

End: 709440

Strand: Direct

Name: bioB [C]

Synonym: MJ0785

Alternate gene names: 15668967

Gene position: 708313-709440 (Clockwise)

Preceding gene: 15668966

Following gene: 15668968

Centisome position: 42.54

GC content: 32.18

Gene sequence:

>1128_bases
GTGAAATATACTTATAAAATAAGTAAAGTAAAAATATCTGGTGATTTAATGGTATTTGGAAAAATTGAAGAAGAGTTTAA
AGAATTTTTAGAAAGTAGAAGAAAATATAACGAATTTATAAAAAATGGGCTTATAGATGAAGATGAGGCATTAAAATTAT
TTAAAATAGACAATTGGAGAGATTATTTAAAGCTATTTGACATTGCCTCAAGGGTTAGGGATTATTTTAAGAAGAAGATT
GAGATCACATCAACAATTCACATAACCAACATCTGCCATGTGAATCCTAAATGCTTATATTGCGGTTTTGCTGCCGGAAC
TTCAAAAGAGGGCTATTATGAGCCGTTTAGATTAACAGATGAGGAGATAAAAAAATCAGCAATAGCCATTGAAGAGAGTG
GGATAAAAAGAGTTAGTTGCTCTTCTGCACATGGTTATCAAGGAAAAGAAGTAATAAGAGCTTTAAAGATAGTTAAAAAA
TACACAAACTTGGAAGTTTTAGTTAATGCAGGGGCTGATTTAACAGAGGAATCTATTAAAGAACTTAAAAAATATGGAAT
TGATACAATATGTTGTAATTTAGAAACAATAAATGAGAATCTTTTTAAAAAGGTTAAGCCGGGAGAGGAGTTGGAAGATA
GAATAAGGGTTTGTAAATTAGTTAATAAATATGATATTGAGTTATCTACTGGCTTATTGATTGGTATTGGGGAGAGTTAT
GAGGATAGAGTAGAGCATTTATTTTATTTAAAAAATGAGTTAAATGTTGGGGAAATTCCAATAATGGGTTTCAACCCTTA
CAAAGGAACTCCTATGGAGAATCATCCAAAATGTTCTGCTTTGGAGCAGGCAAAGACGATAGCTATAACAAGGCTGATAT
TTCCAAATATAAGGATTACATCACCAACACCAACTATAGGGGCTGAGTTGGTGCAGTTTGCATTATTTGGAGGGGCGAGT
AATGTAGCAACGGTTATTCCTAAAAATCATCCAATGAACGTAAAGGGAGTTGGAAATCCAAAAACTGGGAATTTGGAGGA
AGTTGTTAAGATGATTATGGATTTGGGATTAAAGCCAAAGTTGGATTGGAGAAGGTATGAGAATTATTTGAAGATTTATG
GAAAATAA

Upstream 100 bases:

>100_bases
AAAATTGTTTCTTTTTTTGCAAAAAAATACATTAAAAATATTAAAGTCAGAAAATATTAAAAATTATTATTAATATTAAT
GCAAAATAAAATATAAAAGT

Downstream 100 bases:

>100_bases
TAAGTGTGGAACTATGAGGGGAGAAAGTGTTTATTTAGCCTATCCATTTATCTTAGGTAGTGTAATATTTGTTGAATTTT
TTATTTTTGGCTTAGTGTAT

Product: biotin synthase

Products: biotin

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MKYTYKISKVKISGDLMVFGKIEEEFKEFLESRRKYNEFIKNGLIDEDEALKLFKIDNWRDYLKLFDIASRVRDYFKKKI
EITSTIHITNICHVNPKCLYCGFAAGTSKEGYYEPFRLTDEEIKKSAIAIEESGIKRVSCSSAHGYQGKEVIRALKIVKK
YTNLEVLVNAGADLTEESIKELKKYGIDTICCNLETINENLFKKVKPGEELEDRIRVCKLVNKYDIELSTGLLIGIGESY
EDRVEHLFYLKNELNVGEIPIMGFNPYKGTPMENHPKCSALEQAKTIAITRLIFPNIRITSPTPTIGAELVQFALFGGAS
NVATVIPKNHPMNVKGVGNPKTGNLEEVVKMIMDLGLKPKLDWRRYENYLKIYGK

Sequences:

>Translated_375_residues
MKYTYKISKVKISGDLMVFGKIEEEFKEFLESRRKYNEFIKNGLIDEDEALKLFKIDNWRDYLKLFDIASRVRDYFKKKI
EITSTIHITNICHVNPKCLYCGFAAGTSKEGYYEPFRLTDEEIKKSAIAIEESGIKRVSCSSAHGYQGKEVIRALKIVKK
YTNLEVLVNAGADLTEESIKELKKYGIDTICCNLETINENLFKKVKPGEELEDRIRVCKLVNKYDIELSTGLLIGIGESY
EDRVEHLFYLKNELNVGEIPIMGFNPYKGTPMENHPKCSALEQAKTIAITRLIFPNIRITSPTPTIGAELVQFALFGGAS
NVATVIPKNHPMNVKGVGNPKTGNLEEVVKMIMDLGLKPKLDWRRYENYLKIYGK
>Mature_375_residues
MKYTYKISKVKISGDLMVFGKIEEEFKEFLESRRKYNEFIKNGLIDEDEALKLFKIDNWRDYLKLFDIASRVRDYFKKKI
EITSTIHITNICHVNPKCLYCGFAAGTSKEGYYEPFRLTDEEIKKSAIAIEESGIKRVSCSSAHGYQGKEVIRALKIVKK
YTNLEVLVNAGADLTEESIKELKKYGIDTICCNLETINENLFKKVKPGEELEDRIRVCKLVNKYDIELSTGLLIGIGESY
EDRVEHLFYLKNELNVGEIPIMGFNPYKGTPMENHPKCSALEQAKTIAITRLIFPNIRITSPTPTIGAELVQFALFGGAS
NVATVIPKNHPMNVKGVGNPKTGNLEEVVKMIMDLGLKPKLDWRRYENYLKIYGK

Specific function: Biotin biosynthesis; last step. [C]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y785_METJA (Q58195)

Other databases:

- EMBL:   L77117
- PIR:   A64398
- RefSeq:   NP_247771.1
- ProteinModelPortal:   Q58195
- GeneID:   1451663
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0785
- NMPDR:   fig|243232.1.peg.808
- TIGR:   MJ0785
- HOGENOM:   HBG539119
- OMA:   FPNIRIT
- ProtClustDB:   PRK06256
- BioCyc:   MJAN243232:MJ_0785-MONOMER
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: 2.8.1.6

Molecular weight: Translated: 42810; Mature: 42810

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYTYKISKVKISGDLMVFGKIEEEFKEFLESRRKYNEFIKNGLIDEDEALKLFKIDNWR
CCEEEEEEEEEEECCEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCHH
DYLKLFDIASRVRDYFKKKIEITSTIHITNICHVNPKCLYCGFAAGTSKEGYYEPFRLTD
HHHHHHHHHHHHHHHHHHHEEEEEEEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCH
EEIKKSAIAIEESGIKRVSCSSAHGYQGKEVIRALKIVKKYTNLEVLVNAGADLTEESIK
HHHHHHHHEEHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHH
ELKKYGIDTICCNLETINENLFKKVKPGEELEDRIRVCKLVNKYDIELSTGLLIGIGESY
HHHHCCCCEEEECHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEEEECCEEEEECCCH
EDRVEHLFYLKNELNVGEIPIMGFNPYKGTPMENHPKCSALEQAKTIAITRLIFPNIRIT
HHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEC
SPTPTIGAELVQFALFGGASNVATVIPKNHPMNVKGVGNPKTGNLEEVVKMIMDLGLKPK
CCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCEEECCCCCCCCHHHHHHHHHHCCCCCC
LDWRRYENYLKIYGK
CCHHHHHCHHEEECC
>Mature Secondary Structure
MKYTYKISKVKISGDLMVFGKIEEEFKEFLESRRKYNEFIKNGLIDEDEALKLFKIDNWR
CCEEEEEEEEEEECCEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCHH
DYLKLFDIASRVRDYFKKKIEITSTIHITNICHVNPKCLYCGFAAGTSKEGYYEPFRLTD
HHHHHHHHHHHHHHHHHHHEEEEEEEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCH
EEIKKSAIAIEESGIKRVSCSSAHGYQGKEVIRALKIVKKYTNLEVLVNAGADLTEESIK
HHHHHHHHEEHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHH
ELKKYGIDTICCNLETINENLFKKVKPGEELEDRIRVCKLVNKYDIELSTGLLIGIGESY
HHHHCCCCEEEECHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEEEECCEEEEECCCH
EDRVEHLFYLKNELNVGEIPIMGFNPYKGTPMENHPKCSALEQAKTIAITRLIFPNIRIT
HHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEC
SPTPTIGAELVQFALFGGASNVATVIPKNHPMNVKGVGNPKTGNLEEVVKMIMDLGLKPK
CCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCEEECCCCCCCCHHHHHHHHHHCCCCCC
LDWRRYENYLKIYGK
CCHHHHHCHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.002 {dethiobiotin}} [C]

Substrates: dethiobiotin; sulfur

Specific reaction: dethiobiotin + sulfur = biotin

General reaction: Sulfur atom transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087