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The map label for this gene is 15668908

Identifier: 15668908

GI number: 15668908

Start: 659632

End: 660528

Strand: Reverse

Name: 15668908

Synonym: MJ0727

Alternate gene names: NA

Gene position: 660528-659632 (Counterclockwise)

Preceding gene: 15668909

Following gene: 15668907

Centisome position: 39.67

GC content: 32.89

Gene sequence:

>897_bases
ATGAAAATTAGAGGGTTTGAAAGCTCAATGATGGGGAAAGATATAGATTTTATTCCCCCAGCTATGACAAGGTTATGCTG
TTTAAATGAAATCTCCCATGCTTTAGCAGGAGTTATGGCTGTTGAGAAAGCTTATAACATAACAGTTCCAAATGAAGGGC
AGTATTTGAGGGAGATTGCAAGATTGGGGGAGATTGTTGAAGTAGATGCAATTAAGTTGAGAGAATTTAAAAATACAGAT
GATTTAGCAGATATTGGAAACAAAATAAAATCTGTGTTAGGAAAAAAGGCTAAATATTTGGCTGTTGGTGGAGTTTTAGA
AAATATAAGTGATAAAAGAAAAGAAAAATTAATTAATTTGGCAAAAGAGGGATTAAACTTAGTTGATAAAGATTTTGTTA
AGTTAGTTGATGAGAGAAAGGCAAAGATTCCATTGCCAGATGTTGAGTTGATTGATGCTTATAACTTTGATGCTAATAAA
GTGGAAACAAACGGCTTACCAAAAACAGCCCTTTATGATGGAAAGGTAGTTTATAGTGGGTCTTTGGCAAGAATGTATAA
GGAGGGCTTAATAAATTCAAAAAACTTATGGGATGTGTTATCTTCAAGAATGATTGAGATAGAATTCTGCTTAAATAAAA
TTATAGAACTCTTAAACAAATTAAAATTAACACACCCATACATGGAGCCAATTATAAAAGATGGAAAGGCAATTGGGGAG
GCTGTTATAGAAGGAGGAGAGGGAATCGTTTATCACAAAGTTGAGTTACTTGGAAGAGAGATTTTGGATTACACAATATT
AACAAGTGAGAACTTCAACAAAGCAGTTTTGGATAGTGTAGATAATGATGAAGCAAAAAGAATCATTCAGCTCTGTGAAA
GATGCTACTATTTATAA

Upstream 100 bases:

>100_bases
AGCTGATTTATTGGAGCAAACAATCATGGAGAAGAGGAAAAAACTTGGAATCTAATTAAATTCTTTTTAAACTTTTAAAA
CATTTTAAAAAGGTGGAATT

Downstream 100 bases:

>100_bases
GCTAATTAGATAACTACGAAAATAGGGGATAATTTGAACTAATGGACATTAATTAGGGTTGAAAGCCCTAACTTAATGGA
CACGTTTTGGTCAAGCTTTT

Product: coenzyme F420-reducing hydrogenase subunit alpha

Products: reduced coenzyme F420

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MKIRGFESSMMGKDIDFIPPAMTRLCCLNEISHALAGVMAVEKAYNITVPNEGQYLREIARLGEIVEVDAIKLREFKNTD
DLADIGNKIKSVLGKKAKYLAVGGVLENISDKRKEKLINLAKEGLNLVDKDFVKLVDERKAKIPLPDVELIDAYNFDANK
VETNGLPKTALYDGKVVYSGSLARMYKEGLINSKNLWDVLSSRMIEIEFCLNKIIELLNKLKLTHPYMEPIIKDGKAIGE
AVIEGGEGIVYHKVELLGREILDYTILTSENFNKAVLDSVDNDEAKRIIQLCERCYYL

Sequences:

>Translated_298_residues
MKIRGFESSMMGKDIDFIPPAMTRLCCLNEISHALAGVMAVEKAYNITVPNEGQYLREIARLGEIVEVDAIKLREFKNTD
DLADIGNKIKSVLGKKAKYLAVGGVLENISDKRKEKLINLAKEGLNLVDKDFVKLVDERKAKIPLPDVELIDAYNFDANK
VETNGLPKTALYDGKVVYSGSLARMYKEGLINSKNLWDVLSSRMIEIEFCLNKIIELLNKLKLTHPYMEPIIKDGKAIGE
AVIEGGEGIVYHKVELLGREILDYTILTSENFNKAVLDSVDNDEAKRIIQLCERCYYL
>Mature_298_residues
MKIRGFESSMMGKDIDFIPPAMTRLCCLNEISHALAGVMAVEKAYNITVPNEGQYLREIARLGEIVEVDAIKLREFKNTD
DLADIGNKIKSVLGKKAKYLAVGGVLENISDKRKEKLINLAKEGLNLVDKDFVKLVDERKAKIPLPDVELIDAYNFDANK
VETNGLPKTALYDGKVVYSGSLARMYKEGLINSKNLWDVLSSRMIEIEFCLNKIIELLNKLKLTHPYMEPIIKDGKAIGE
AVIEGGEGIVYHKVELLGREILDYTILTSENFNKAVLDSVDNDEAKRIIQLCERCYYL

Specific function: Unknown

COG id: COG3259

COG function: function code C; Coenzyme F420-reducing hydrogenase, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y727_METJA (Q58137)

Other databases:

- EMBL:   L77117
- PIR:   G64390
- RefSeq:   NP_247712.1
- ProteinModelPortal:   Q58137
- GeneID:   1451604
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0727
- NMPDR:   fig|243232.1.peg.749
- TIGR:   MJ0727
- OMA:   CLNEISH
- ProtClustDB:   CLSK2517649
- BioCyc:   MJAN243232:MJ_0727-MONOMER
- InterPro:   IPR001501

Pfam domain/function: PF00374 NiFeSe_Hases

EC number: 1.12.98.1

Molecular weight: Translated: 33520; Mature: 33520

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRGFESSMMGKDIDFIPPAMTRLCCLNEISHALAGVMAVEKAYNITVPNEGQYLREIA
CCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEECCCCCCHHHHHHH
RLGEIVEVDAIKLREFKNTDDLADIGNKIKSVLGKKAKYLAVGGVLENISDKRKEKLINL
HHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
AKEGLNLVDKDFVKLVDERKAKIPLPDVELIDAYNFDANKVETNGLPKTALYDGKVVYSG
HHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCEECCCCCCCEEECCEEEECC
SLARMYKEGLINSKNLWDVLSSRMIEIEFCLNKIIELLNKLKLTHPYMEPIIKDGKAIGE
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
AVIEGGEGIVYHKVELLGREILDYTILTSENFNKAVLDSVDNDEAKRIIQLCERCYYL
HHHHCCCCEEEHHHHHHHHHHHHHHEECCCCCCHHHHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKIRGFESSMMGKDIDFIPPAMTRLCCLNEISHALAGVMAVEKAYNITVPNEGQYLREIA
CCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEECCCCCCHHHHHHH
RLGEIVEVDAIKLREFKNTDDLADIGNKIKSVLGKKAKYLAVGGVLENISDKRKEKLINL
HHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
AKEGLNLVDKDFVKLVDERKAKIPLPDVELIDAYNFDANKVETNGLPKTALYDGKVVYSG
HHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCEECCCCCCCEEECCEEEECC
SLARMYKEGLINSKNLWDVLSSRMIEIEFCLNKIIELLNKLKLTHPYMEPIIKDGKAIGE
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
AVIEGGEGIVYHKVELLGREILDYTILTSENFNKAVLDSVDNDEAKRIIQLCERCYYL
HHHHCCCCEEEHHHHHHHHHHHHHHEECCCCCCHHHHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2; coenzyme F420

Specific reaction: H2 + coenzyme F420 = reduced coenzyme F420

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087