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The map label for this gene is polB

Identifier: 15668883

GI number: 15668883

Start: 628049

End: 629833

Strand: Direct

Name: polB

Synonym: MJ0702

Alternate gene names: 15668883

Gene position: 628049-629833 (Clockwise)

Preceding gene: 15668882

Following gene: 15668884

Centisome position: 37.72

GC content: 32.49

Gene sequence:

>1785_bases
ATGGAGATAATAAATAAATTCTTAGATTTAGAGGCTTTATTATCACCAACTGTTTATGAAAAGTTAAAAAATTTTGATGA
AGAAAAATTAAAAAGACTAATTCAAAAAATTAGAGAATTTAAAAAATATAACAACGCTTTTATTTTGTTAGATGAGAAGT
TTTTAGACATCTTTTTACAAAAAGATTTGGATGAAATAATAAATGAATATAAGGATTTTGACTTTATATTTTACTACACT
GGAGAAGAAGAAAAAGAAAAACCTAAAGAAGTAAAAAAAGAGATTAAAAAAGAAACTGAAGAGAAAATAGAAAAAGAAAA
AATAGAATTTGTTAAGAAAGAAGAAAAAGAACAATTTATAAAAAAATCTGATGAAGATGTTGAAGAGAAATTAAAACAAC
TAATTTCCAAAGAAGAGAAAAAAGAAGATTTCGATGCTGAGAGGGCTAAAAGATATGAACACATAACAAAAATAAAAGAG
AGTGTAAATAGTAGAATAAAATGGATAGCTAAAGACATCGATGCCGTGATTGAGATATATGAAGATTCAGACGTGTCTGG
AAAATCCACATGCACTGGAACTATTGAGGACTTCGTTAAATACTTTAGAGACAGATTTGAAAGATTAAAGGTTTTTATTG
AGAGAAAAGCTCAAAGAAAGGGATATCCTCTAAAAGATATAAAGAAAATGAAAGGACAGAAGGATATTTTTGTCGTAGGA
ATCGTTAGTGATGTTGATAGTACAAGAAATGGGAACTTGATAGTTAGGATTGAAGACACCGAAGATGAAGCAACGTTAAT
TCTGCCAAAAGAAAAAATCGAAGCTGGAAAAATACCTGACGATATTTTGTTAGATGAAGTTATTGGAGCTATTGGGACTG
TTAGCAAATCTGGAAGTTCAATATACGTTGATGAAATTATACGTCCAGCATTACCACCAAAAGAACCAAAGAGAATTGAT
GAAGAGATATATATGGCATTTTTATCTGATATTCACGTTGGAAGTAAGGAGTTTTTGCATAAAGAGTTTGAAAAATTCAT
CAGATTTTTAAATGGAGATGTTGATAATGAATTAGAGGAAAAGGTCGTTAGCAGATTAAAATACATCTGCATAGCTGGGG
ATTTAGTTGATGGGGTTGGTGTCTATCCAGGGCAGGAAGAGGATTTGTATGAGGTAGATATTATTGAGCAGTATAGAGAA
ATAGCAATGTATTTAGATCAGATTCCAGAGCATATAAGCATAATCATCTCCCCAGGAAACCACGATGCTGTTAGACCAGC
AGAACCTCAACCAAAACTGCCAGAGAAAATAACTAAGCTATTTAATAGAGATAACATCTACTTCGTTGGAAACCCATGCA
CTCTCAACATCCATGGCTTTGATACTCTATTATATCACGGCAGAAGCTTTGACGACTTAGTTGGACAAATAAGGGCTGCA
AGTTATGAAAATCCAGTAACTATTATGAAGGAGTTGATAAAAAGAAGGCTATTATGTCCAACTTATGGAGGAAGATGTCC
TATAGCTCCAGAACATAAAGATTACTTGGTTATAGATAGGGATATTGATATTTTACACACTGGGCATATACACATCAACG
GTTATGGAATTTATAGAGGAGTTGTTATGGTTAATAGCGGGACATTCCAAGAGCAAACAGATTTCCAGAAGAGGATGGGT
ATTAGCCCAACACCTGCAATAGTTCCAATAATAAACATGGCTAAGGTTGGGGAGAAGGGGCATTATTTAGAATGGGATAG
GGGAGTTTTAGAGGTTAGATATTAA

Upstream 100 bases:

>100_bases
AAGAAGAAATTTATCATTCATCCATTGGATTATCTCTGCTACAATATAAATAAACATTTGAAGAAAGAGAGATTTAAGAA
GATAAAGAAGGAGGATAAAA

Downstream 100 bases:

>100_bases
TAAATAATAATTTAGAGGGAAACTATGAAAATAATCCCAGTCATTGATTTAAAAGACAAAATAGCTGTGCATGGAAAGAG
TGGAAATAGAGATGAATATA

Product: DNA polymerase II small subunit

Products: NA

Alternate protein names: Pol II

Number of amino acids: Translated: 594; Mature: 594

Protein sequence:

>594_residues
MEIINKFLDLEALLSPTVYEKLKNFDEEKLKRLIQKIREFKKYNNAFILLDEKFLDIFLQKDLDEIINEYKDFDFIFYYT
GEEEKEKPKEVKKEIKKETEEKIEKEKIEFVKKEEKEQFIKKSDEDVEEKLKQLISKEEKKEDFDAERAKRYEHITKIKE
SVNSRIKWIAKDIDAVIEIYEDSDVSGKSTCTGTIEDFVKYFRDRFERLKVFIERKAQRKGYPLKDIKKMKGQKDIFVVG
IVSDVDSTRNGNLIVRIEDTEDEATLILPKEKIEAGKIPDDILLDEVIGAIGTVSKSGSSIYVDEIIRPALPPKEPKRID
EEIYMAFLSDIHVGSKEFLHKEFEKFIRFLNGDVDNELEEKVVSRLKYICIAGDLVDGVGVYPGQEEDLYEVDIIEQYRE
IAMYLDQIPEHISIIISPGNHDAVRPAEPQPKLPEKITKLFNRDNIYFVGNPCTLNIHGFDTLLYHGRSFDDLVGQIRAA
SYENPVTIMKELIKRRLLCPTYGGRCPIAPEHKDYLVIDRDIDILHTGHIHINGYGIYRGVVMVNSGTFQEQTDFQKRMG
ISPTPAIVPIINMAKVGEKGHYLEWDRGVLEVRY

Sequences:

>Translated_594_residues
MEIINKFLDLEALLSPTVYEKLKNFDEEKLKRLIQKIREFKKYNNAFILLDEKFLDIFLQKDLDEIINEYKDFDFIFYYT
GEEEKEKPKEVKKEIKKETEEKIEKEKIEFVKKEEKEQFIKKSDEDVEEKLKQLISKEEKKEDFDAERAKRYEHITKIKE
SVNSRIKWIAKDIDAVIEIYEDSDVSGKSTCTGTIEDFVKYFRDRFERLKVFIERKAQRKGYPLKDIKKMKGQKDIFVVG
IVSDVDSTRNGNLIVRIEDTEDEATLILPKEKIEAGKIPDDILLDEVIGAIGTVSKSGSSIYVDEIIRPALPPKEPKRID
EEIYMAFLSDIHVGSKEFLHKEFEKFIRFLNGDVDNELEEKVVSRLKYICIAGDLVDGVGVYPGQEEDLYEVDIIEQYRE
IAMYLDQIPEHISIIISPGNHDAVRPAEPQPKLPEKITKLFNRDNIYFVGNPCTLNIHGFDTLLYHGRSFDDLVGQIRAA
SYENPVTIMKELIKRRLLCPTYGGRCPIAPEHKDYLVIDRDIDILHTGHIHINGYGIYRGVVMVNSGTFQEQTDFQKRMG
ISPTPAIVPIINMAKVGEKGHYLEWDRGVLEVRY
>Mature_594_residues
MEIINKFLDLEALLSPTVYEKLKNFDEEKLKRLIQKIREFKKYNNAFILLDEKFLDIFLQKDLDEIINEYKDFDFIFYYT
GEEEKEKPKEVKKEIKKETEEKIEKEKIEFVKKEEKEQFIKKSDEDVEEKLKQLISKEEKKEDFDAERAKRYEHITKIKE
SVNSRIKWIAKDIDAVIEIYEDSDVSGKSTCTGTIEDFVKYFRDRFERLKVFIERKAQRKGYPLKDIKKMKGQKDIFVVG
IVSDVDSTRNGNLIVRIEDTEDEATLILPKEKIEAGKIPDDILLDEVIGAIGTVSKSGSSIYVDEIIRPALPPKEPKRID
EEIYMAFLSDIHVGSKEFLHKEFEKFIRFLNGDVDNELEEKVVSRLKYICIAGDLVDGVGVYPGQEEDLYEVDIIEQYRE
IAMYLDQIPEHISIIISPGNHDAVRPAEPQPKLPEKITKLFNRDNIYFVGNPCTLNIHGFDTLLYHGRSFDDLVGQIRAA
SYENPVTIMKELIKRRLLCPTYGGRCPIAPEHKDYLVIDRDIDILHTGHIHINGYGIYRGVVMVNSGTFQEQTDFQKRMG
ISPTPAIVPIINMAKVGEKGHYLEWDRGVLEVRY

Specific function: Possesses two activities:a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3' to 5' direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase

COG id: COG1311

COG function: function code L; Archaeal DNA polymerase II, small subunit/DNA polymerase delta, subunit B

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA polymerase delta/II small subunit family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DP2S_METJA (Q58113)

Other databases:

- EMBL:   L77117
- PIR:   F64387
- RefSeq:   NP_247686.1
- ProteinModelPortal:   Q58113
- GeneID:   1451569
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0702
- NMPDR:   fig|243232.1.peg.723
- TIGR:   MJ0702
- HOGENOM:   HBG540972
- OMA:   CPIAPEH
- ProtClustDB:   PRK04036
- BioCyc:   MJAN243232:MJ_0702-MONOMER
- BRENDA:   2.7.7.7
- HAMAP:   MF_00325
- InterPro:   IPR004843
- InterPro:   IPR011149
- PIRSF:   PIRSF000803

Pfam domain/function: PF00149 Metallophos

EC number: =2.7.7.7

Molecular weight: Translated: 69146; Mature: 69146

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIINKFLDLEALLSPTVYEKLKNFDEEKLKRLIQKIREFKKYNNAFILLDEKFLDIFLQ
CCHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHH
KDLDEIINEYKDFDFIFYYTGEEEKEKPKEVKKEIKKETEEKIEKEKIEFVKKEEKEQFI
HHHHHHHHHHCCCCEEEEECCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKSDEDVEEKLKQLISKEEKKEDFDAERAKRYEHITKIKESVNSRIKWIAKDIDAVIEIY
HCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EDSDVSGKSTCTGTIEDFVKYFRDRFERLKVFIERKAQRKGYPLKDIKKMKGQKDIFVVG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEEEEE
IVSDVDSTRNGNLIVRIEDTEDEATLILPKEKIEAGKIPDDILLDEVIGAIGTVSKSGSS
EEECCCCCCCCCEEEEEECCCCCEEEEEEHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCE
IYVDEIIRPALPPKEPKRIDEEIYMAFLSDIHVGSKEFLHKEFEKFIRFLNGDVDNELEE
EEHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHH
KVVSRLKYICIAGDLVDGVGVYPGQEEDLYEVDIIEQYREIAMYLDQIPEHISIIISPGN
HHHHHHHHHHEECHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCC
HDAVRPAEPQPKLPEKITKLFNRDNIYFVGNPCTLNIHGFDTLLYHGRSFDDLVGQIRAA
CCCCCCCCCCCCCHHHHHHHHCCCCEEEEECCEEEEECCHHHHHHCCCCHHHHHHHHHHC
SYENPVTIMKELIKRRLLCPTYGGRCPIAPEHKDYLVIDRDIDILHTGHIHINGYGIYRG
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCEEEEECEEEEEEEEEEEE
VVMVNSGTFQEQTDFQKRMGISPTPAIVPIINMAKVGEKGHYLEWDRGVLEVRY
EEEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCEEECCCCEEEECC
>Mature Secondary Structure
MEIINKFLDLEALLSPTVYEKLKNFDEEKLKRLIQKIREFKKYNNAFILLDEKFLDIFLQ
CCHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHH
KDLDEIINEYKDFDFIFYYTGEEEKEKPKEVKKEIKKETEEKIEKEKIEFVKKEEKEQFI
HHHHHHHHHHCCCCEEEEECCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKSDEDVEEKLKQLISKEEKKEDFDAERAKRYEHITKIKESVNSRIKWIAKDIDAVIEIY
HCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EDSDVSGKSTCTGTIEDFVKYFRDRFERLKVFIERKAQRKGYPLKDIKKMKGQKDIFVVG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEEEEE
IVSDVDSTRNGNLIVRIEDTEDEATLILPKEKIEAGKIPDDILLDEVIGAIGTVSKSGSS
EEECCCCCCCCCEEEEEECCCCCEEEEEEHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCE
IYVDEIIRPALPPKEPKRIDEEIYMAFLSDIHVGSKEFLHKEFEKFIRFLNGDVDNELEE
EEHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHH
KVVSRLKYICIAGDLVDGVGVYPGQEEDLYEVDIIEQYREIAMYLDQIPEHISIIISPGN
HHHHHHHHHHEECHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCC
HDAVRPAEPQPKLPEKITKLFNRDNIYFVGNPCTLNIHGFDTLLYHGRSFDDLVGQIRAA
CCCCCCCCCCCCCHHHHHHHHCCCCEEEEECCEEEEECCHHHHHHCCCCHHHHHHHHHHC
SYENPVTIMKELIKRRLLCPTYGGRCPIAPEHKDYLVIDRDIDILHTGHIHINGYGIYRG
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCEEEEECEEEEEEEEEEEE
VVMVNSGTFQEQTDFQKRMGISPTPAIVPIINMAKVGEKGHYLEWDRGVLEVRY
EEEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCEEECCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087