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The map label for this gene is argG

Identifier: 15668605

GI number: 15668605

Start: 385151

End: 386338

Strand: Direct

Name: argG

Synonym: MJ0429

Alternate gene names: 15668605

Gene position: 385151-386338 (Clockwise)

Preceding gene: 15668601

Following gene: 15668614

Centisome position: 23.13

GC content: 35.61

Gene sequence:

>1188_bases
ATGGAGAGAATAGCTGTCTTAGCGTATTCTGGAGGATTGGATACAAGCTGTTGCTTAAAATTATTGGAAGATAAGTATGG
TTATAAGGTAGTTTCTGTCTGTGTGGATGTTGGACAGCCAGAAGAAGAGATAAAAGAAGTTGAAGAGAAAGCTAAAAAAT
TAGGAGTTTTAAAGCACTACACAATAGATGCAAAGGAAGAATTCGTTAAAGATTACATATTTAGAGCTATAAAGGCAAAT
GCAATGTATGAAGGCTATCCCCTATCAACAGCATTAGCGAGACCTTTAATTGCTCATAAGGTTGTTGAAATAGCTGAGGA
AGTTGGAGCTGAGGCAGTTGCTCATGGATGCACTGGAAAGGGTAACGACCAGTTCAGATTTGAAACAACTATAAGAATTA
AAGCTCCACATTTGAAAATTATTGCACCAATTAGGGACTTAAACCTAACAAGGGCTGAAGAAATTGAGTATGCTAAAGAA
AAAGGAATCCCAATCCCAACGGAAAGTAAAAAATACAGTATAGATGAAAACTTATGGGGAAGAAGTATTGAAGGTAGTGA
GTTAGAAAACCCTGACTTTGTTCCACCAGAAGAGATATATGCATGGACTAAAAACCCAGTTGAAGATAAAGAGGAAGAGA
TTGTTGAGATTGAGTTTAAGGAGGGCGTTCCAGTAGCTATAAATGGAGAAAAATTAGAACCAGTTGAGTTAATAAAGAAA
GCTAATGAGATTGCTGGAAAGCATGGTGTTGGAAGAATAGATATTATTGAGGATAGAATCATAGGATTAAAATCAAGAGA
AAACTATGAATGTCCTGGAGCTGTTTTGTTATTAACTGCTCATAAAGCTTTAGAGCAGTTAGTTTTAACAAGGGATGAGC
TTAGATTTAAAGAGATCGTTGATAGTTTATACGGAGAGTTAATTTATAAAGGACTTTGGTTTGATCCATTAAGGGAGGAT
TTAGATGCTTTTATAGACAAAACTCAAGAGAGAGTTACTGGAACTGTGAAAGTTAAGTTATTTGGAGGAACTGCAAGAGT
TGTTGGTAGAGATAGTCCTTACGCTTTATACAGTAAGGAATTAGTTTCATTTGATGAGAAGGAGATTGACCAGAAAGAAT
TGGCTGGAATGGTTAAGTATCATGGATTACAGGCAATGTTATATGAGATGAGGAAAAAGAGAAAATAA

Upstream 100 bases:

>100_bases
GTGAGTTATATATACTCCAAAACTATAAGCAATAAAATACAAAAAGTTTAAGGATTTATAATTTTATGTAAGATATAAAA
ATATTTTGTGAGGGATTGTC

Downstream 100 bases:

>100_bases
TTTCTCTTTAATCTTTTTTATGATTGTCTAAATGTATTAAAGAAGGCATAACACTTTTTTGATAGGCATATTTTGATGTT
TTTCTTTCTGAATAACCAAC

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MERIAVLAYSGGLDTSCCLKLLEDKYGYKVVSVCVDVGQPEEEIKEVEEKAKKLGVLKHYTIDAKEEFVKDYIFRAIKAN
AMYEGYPLSTALARPLIAHKVVEIAEEVGAEAVAHGCTGKGNDQFRFETTIRIKAPHLKIIAPIRDLNLTRAEEIEYAKE
KGIPIPTESKKYSIDENLWGRSIEGSELENPDFVPPEEIYAWTKNPVEDKEEEIVEIEFKEGVPVAINGEKLEPVELIKK
ANEIAGKHGVGRIDIIEDRIIGLKSRENYECPGAVLLLTAHKALEQLVLTRDELRFKEIVDSLYGELIYKGLWFDPLRED
LDAFIDKTQERVTGTVKVKLFGGTARVVGRDSPYALYSKELVSFDEKEIDQKELAGMVKYHGLQAMLYEMRKKRK

Sequences:

>Translated_395_residues
MERIAVLAYSGGLDTSCCLKLLEDKYGYKVVSVCVDVGQPEEEIKEVEEKAKKLGVLKHYTIDAKEEFVKDYIFRAIKAN
AMYEGYPLSTALARPLIAHKVVEIAEEVGAEAVAHGCTGKGNDQFRFETTIRIKAPHLKIIAPIRDLNLTRAEEIEYAKE
KGIPIPTESKKYSIDENLWGRSIEGSELENPDFVPPEEIYAWTKNPVEDKEEEIVEIEFKEGVPVAINGEKLEPVELIKK
ANEIAGKHGVGRIDIIEDRIIGLKSRENYECPGAVLLLTAHKALEQLVLTRDELRFKEIVDSLYGELIYKGLWFDPLRED
LDAFIDKTQERVTGTVKVKLFGGTARVVGRDSPYALYSKELVSFDEKEIDQKELAGMVKYHGLQAMLYEMRKKRK
>Mature_395_residues
MERIAVLAYSGGLDTSCCLKLLEDKYGYKVVSVCVDVGQPEEEIKEVEEKAKKLGVLKHYTIDAKEEFVKDYIFRAIKAN
AMYEGYPLSTALARPLIAHKVVEIAEEVGAEAVAHGCTGKGNDQFRFETTIRIKAPHLKIIAPIRDLNLTRAEEIEYAKE
KGIPIPTESKKYSIDENLWGRSIEGSELENPDFVPPEEIYAWTKNPVEDKEEEIVEIEFKEGVPVAINGEKLEPVELIKK
ANEIAGKHGVGRIDIIEDRIIGLKSRENYECPGAVLLLTAHKALEQLVLTRDELRFKEIVDSLYGELIYKGLWFDPLRED
LDAFIDKTQERVTGTVKVKLFGGTARVVGRDSPYALYSKELVSFDEKEIDQKELAGMVKYHGLQAMLYEMRKKRK

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=402, Percent_Identity=43.0348258706468, Blast_Score=280, Evalue=2e-75,
Organism=Homo sapiens, GI16950633, Length=402, Percent_Identity=43.0348258706468, Blast_Score=280, Evalue=2e-75,
Organism=Escherichia coli, GI1789563, Length=360, Percent_Identity=31.3888888888889, Blast_Score=129, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6324514, Length=391, Percent_Identity=38.3631713554987, Blast_Score=243, Evalue=4e-65,
Organism=Drosophila melanogaster, GI21358151, Length=377, Percent_Identity=40.8488063660477, Blast_Score=261, Evalue=6e-70,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_METJA (Q60174)

Other databases:

- EMBL:   L77117
- PIR:   E64353
- RefSeq:   NP_247403.1
- ProteinModelPortal:   Q60174
- SMR:   Q60174
- GeneID:   1451289
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0429
- NMPDR:   fig|243232.1.peg.440
- TIGR:   MJ0429
- HOGENOM:   HBG335267
- OMA:   VAVDVGQ
- ProtClustDB:   PRK13820
- BioCyc:   MJAN243232:MJ_0429-MONOMER
- BRENDA:   6.3.4.5
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 44724; Mature: 44724

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 86-86 BINDING 116-116 BINDING 118-118 BINDING 122-122 BINDING 122-122 BINDING 123-123 BINDING 126-126 BINDING 173-173 BINDING 182-182 BINDING 257-257 BINDING 269-269

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERIAVLAYSGGLDTSCCLKLLEDKYGYKVVSVCVDVGQPEEEIKEVEEKAKKLGVLKHY
CCCEEEEEECCCCCHHHHHHHHHHHCCCEEEEHHHHCCCCHHHHHHHHHHHHHCCCHHEE
TIDAKEEFVKDYIFRAIKANAMYEGYPLSTALARPLIAHKVVEIAEEVGAEAVAHGCTGK
ECCHHHHHHHHHHHHHHHHCCEECCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCC
GNDQFRFETTIRIKAPHLKIIAPIRDLNLTRAEEIEYAKEKGIPIPTESKKYSIDENLWG
CCCCEEEEEEEEEECCCEEEEECHHCCCCCHHHHHHHHHHCCCCCCCCCCCEECCCCCCC
RSIEGSELENPDFVPPEEIYAWTKNPVEDKEEEIVEIEFKEGVPVAINGEKLEPVELIKK
CCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCEEEEEECCCCCEEECCCCCCHHHHHHH
ANEIAGKHGVGRIDIIEDRIIGLKSRENYECPGAVLLLTAHKALEQLVLTRDELRFKEIV
HHHHHCCCCCCEEEEEHHHHCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHH
DSLYGELIYKGLWFDPLREDLDAFIDKTQERVTGTVKVKLFGGTARVVGRDSPYALYSKE
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCEEEEEEEEECCEEEEEECCCCHHHHHHH
LVSFDEKEIDQKELAGMVKYHGLQAMLYEMRKKRK
HHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MERIAVLAYSGGLDTSCCLKLLEDKYGYKVVSVCVDVGQPEEEIKEVEEKAKKLGVLKHY
CCCEEEEEECCCCCHHHHHHHHHHHCCCEEEEHHHHCCCCHHHHHHHHHHHHHCCCHHEE
TIDAKEEFVKDYIFRAIKANAMYEGYPLSTALARPLIAHKVVEIAEEVGAEAVAHGCTGK
ECCHHHHHHHHHHHHHHHHCCEECCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCC
GNDQFRFETTIRIKAPHLKIIAPIRDLNLTRAEEIEYAKEKGIPIPTESKKYSIDENLWG
CCCCEEEEEEEEEECCCEEEEECHHCCCCCHHHHHHHHHHCCCCCCCCCCCEECCCCCCC
RSIEGSELENPDFVPPEEIYAWTKNPVEDKEEEIVEIEFKEGVPVAINGEKLEPVELIKK
CCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCEEEEEECCCCCEEECCCCCCHHHHHHH
ANEIAGKHGVGRIDIIEDRIIGLKSRENYECPGAVLLLTAHKALEQLVLTRDELRFKEIV
HHHHHCCCCCCEEEEEHHHHCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHH
DSLYGELIYKGLWFDPLREDLDAFIDKTQERVTGTVKVKLFGGTARVVGRDSPYALYSKE
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCEEEEEEEEECCEEEEEECCCCHHHHHHH
LVSFDEKEIDQKELAGMVKYHGLQAMLYEMRKKRK
HHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087