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The map label for this gene is tuf

Identifier: 15668498

GI number: 15668498

Start: 299525

End: 300811

Strand: Reverse

Name: tuf

Synonym: MJ0324

Alternate gene names: 15668498

Gene position: 300811-299525 (Counterclockwise)

Preceding gene: 15668499

Following gene: 15668497

Centisome position: 18.07

GC content: 40.79

Gene sequence:

>1287_bases
ATGGCAAAGCAAAAACCAGTATTAAACGTAGCATTCATTGGACACGTCGATGCAGGTAAGTCAACAACAGTCGGTAGATT
ATTATACGACAGTGGAGCTATCGACCCACAGTTATTAGAGAAGTTAAAAAGAGAAGCTCAAGAGAGAGGTAAAGCAGGAT
TCGAGTTTGCTTACGTCATGGACAACTTGAAAGAAGAGAGAGAAAGAGGGGTTACAATTGACGTAGCTCACAAGAAGTTC
GAAACCCAAAAATATGAAGTTACAATCGTCGATTGTCCAGGACACAGGGACTTCATTAAAAACATGATTACAGGAGCTTC
ACAGGCAGACGCTGCTGTCTTAGTTGTTGATGTTAATGATGCCAAGACAGGAATTCAGCCACAAACAAGAGAGCACATGT
TCTTAGCAAGAACATTGGGTATTAAGCAAATTGCAGTTGCAATTAACAAGATGGATACAGTTAACTACAGCCAAGAAGAA
TACGAAAAAATGAAAAAGATGTTATCAGAGCAGTTATTAAAAGTCTTAGGTTACAACCCAGACCAAATTGACTTCATCCC
AACAGCTTCATTGAAAGGAGACAACGTCGTTAAAAGATCAGAAAACATGCCATGGTACAAAGGTCCAACATTAGTTGAAG
CATTAGACAAATTCCAACCACCAGAAAAACCAACAAACTTACCATTAAGAATCCCAATCCAAGATGTCTATTCAATTACA
GGGGTTGGAACTGTCCCAGTTGGAAGAGTCGAAACAGGTATCTTAAGACCAGGAGACAAAGTTGTCTTCGAACCAGCAGG
AGTTAGCGGAGAAGTTAAGTCAATTGAGATGCACCACGAACAAATTCCACAAGCAGAACCAGGAGACAACATTGGATTCA
ACGTTAGAGGAGTCAGTAAGAAAGATATTAAGAGAGGAGACGTTTGTGGGCACCCAGACAACCCACCAACAGTTGCAGAA
GAATTCACAGCTCAAATCGTTGTCTTACAGCACCCAACAGCAATTACAGTTGGTTACACACCAGTCTTCCACGCACACAC
AGCACAGGTTGCATGTACATTCATTGAGTTGTTGAAGAAATTAGACCCAAGAACAGGGCAAGTCATTGAAGAGAACCCAC
AGTTCTTAAAGACTGGTGACGCAGCAATAGTCAAAATCAAACCAACAAAACCAATGGTCATTGAAAACGTTAGAGAAATT
CCACAGTTAGGTAGATTCGCTATCAGAGATATGGGTATGACAATCGCTGCAGGTATGGCAATCGATGTCAAAGCTAAGAA
CAAATAA

Upstream 100 bases:

>100_bases
TCTTATATTCCTACCCTGTTAAGTTTTAACTTAACACCATTTTAGAATAAATATAATAAAAATAAAAAAGATAAAAAAGA
AGGTAAATTGGTGATGAAAT

Downstream 100 bases:

>100_bases
ATTCCTTAAATTTCCCTTTTAATAGCTTTTAAATCCCATTTTATATTTTTTTAATATTTTAAAGCATTTGAGAGGGGAGA
GTATGCAAAGGGCAAGAATC

Product: elongation factor 1-alpha

Products: GDP; phosphate

Alternate protein names: EF-1-alpha; Elongation factor Tu; EF-Tu

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MAKQKPVLNVAFIGHVDAGKSTTVGRLLYDSGAIDPQLLEKLKREAQERGKAGFEFAYVMDNLKEERERGVTIDVAHKKF
ETQKYEVTIVDCPGHRDFIKNMITGASQADAAVLVVDVNDAKTGIQPQTREHMFLARTLGIKQIAVAINKMDTVNYSQEE
YEKMKKMLSEQLLKVLGYNPDQIDFIPTASLKGDNVVKRSENMPWYKGPTLVEALDKFQPPEKPTNLPLRIPIQDVYSIT
GVGTVPVGRVETGILRPGDKVVFEPAGVSGEVKSIEMHHEQIPQAEPGDNIGFNVRGVSKKDIKRGDVCGHPDNPPTVAE
EFTAQIVVLQHPTAITVGYTPVFHAHTAQVACTFIELLKKLDPRTGQVIEENPQFLKTGDAAIVKIKPTKPMVIENVREI
PQLGRFAIRDMGMTIAAGMAIDVKAKNK

Sequences:

>Translated_428_residues
MAKQKPVLNVAFIGHVDAGKSTTVGRLLYDSGAIDPQLLEKLKREAQERGKAGFEFAYVMDNLKEERERGVTIDVAHKKF
ETQKYEVTIVDCPGHRDFIKNMITGASQADAAVLVVDVNDAKTGIQPQTREHMFLARTLGIKQIAVAINKMDTVNYSQEE
YEKMKKMLSEQLLKVLGYNPDQIDFIPTASLKGDNVVKRSENMPWYKGPTLVEALDKFQPPEKPTNLPLRIPIQDVYSIT
GVGTVPVGRVETGILRPGDKVVFEPAGVSGEVKSIEMHHEQIPQAEPGDNIGFNVRGVSKKDIKRGDVCGHPDNPPTVAE
EFTAQIVVLQHPTAITVGYTPVFHAHTAQVACTFIELLKKLDPRTGQVIEENPQFLKTGDAAIVKIKPTKPMVIENVREI
PQLGRFAIRDMGMTIAAGMAIDVKAKNK
>Mature_427_residues
AKQKPVLNVAFIGHVDAGKSTTVGRLLYDSGAIDPQLLEKLKREAQERGKAGFEFAYVMDNLKEERERGVTIDVAHKKFE
TQKYEVTIVDCPGHRDFIKNMITGASQADAAVLVVDVNDAKTGIQPQTREHMFLARTLGIKQIAVAINKMDTVNYSQEEY
EKMKKMLSEQLLKVLGYNPDQIDFIPTASLKGDNVVKRSENMPWYKGPTLVEALDKFQPPEKPTNLPLRIPIQDVYSITG
VGTVPVGRVETGILRPGDKVVFEPAGVSGEVKSIEMHHEQIPQAEPGDNIGFNVRGVSKKDIKRGDVCGHPDNPPTVAEE
FTAQIVVLQHPTAITVGYTPVFHAHTAQVACTFIELLKKLDPRTGQVIEENPQFLKTGDAAIVKIKPTKPMVIENVREIP
QLGRFAIRDMGMTIAAGMAIDVKAKNK

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG5256

COG function: function code J; Translation elongation factor EF-1alpha (GTPase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI4503471, Length=446, Percent_Identity=54.0358744394619, Blast_Score=489, Evalue=1e-138,
Organism=Homo sapiens, GI4503475, Length=446, Percent_Identity=52.6905829596413, Blast_Score=486, Evalue=1e-137,
Organism=Homo sapiens, GI223555963, Length=427, Percent_Identity=39.1100702576112, Blast_Score=327, Evalue=1e-89,
Organism=Homo sapiens, GI5729864, Length=427, Percent_Identity=39.1100702576112, Blast_Score=327, Evalue=2e-89,
Organism=Homo sapiens, GI194018520, Length=429, Percent_Identity=37.5291375291375, Blast_Score=295, Evalue=5e-80,
Organism=Homo sapiens, GI194097354, Length=429, Percent_Identity=37.5291375291375, Blast_Score=295, Evalue=5e-80,
Organism=Homo sapiens, GI194018522, Length=429, Percent_Identity=37.5291375291375, Blast_Score=295, Evalue=6e-80,
Organism=Homo sapiens, GI46094014, Length=429, Percent_Identity=36.5967365967366, Blast_Score=289, Evalue=4e-78,
Organism=Homo sapiens, GI34147630, Length=434, Percent_Identity=31.7972350230415, Blast_Score=189, Evalue=5e-48,
Organism=Homo sapiens, GI54607086, Length=373, Percent_Identity=27.0777479892761, Blast_Score=102, Evalue=9e-22,
Organism=Homo sapiens, GI82546879, Length=403, Percent_Identity=27.5434243176179, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI45593140, Length=420, Percent_Identity=25.2380952380952, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI157426893, Length=315, Percent_Identity=27.9365079365079, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI94966754, Length=180, Percent_Identity=28.8888888888889, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1789108, Length=429, Percent_Identity=32.6340326340326, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1790412, Length=434, Percent_Identity=33.1797235023041, Blast_Score=179, Evalue=4e-46,
Organism=Escherichia coli, GI1789737, Length=434, Percent_Identity=33.1797235023041, Blast_Score=178, Evalue=5e-46,
Organism=Escherichia coli, GI2367247, Length=328, Percent_Identity=27.4390243902439, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI48994988, Length=258, Percent_Identity=30.6201550387597, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788922, Length=310, Percent_Identity=25.1612903225806, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17552884, Length=438, Percent_Identity=52.7397260273973, Blast_Score=474, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI17569207, Length=438, Percent_Identity=52.7397260273973, Blast_Score=474, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI32566303, Length=434, Percent_Identity=49.7695852534562, Blast_Score=439, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI115532067, Length=422, Percent_Identity=37.4407582938389, Blast_Score=290, Evalue=1e-78,
Organism=Caenorhabditis elegans, GI115532065, Length=422, Percent_Identity=37.4407582938389, Blast_Score=288, Evalue=4e-78,
Organism=Caenorhabditis elegans, GI32566629, Length=426, Percent_Identity=35.9154929577465, Blast_Score=276, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI32566301, Length=148, Percent_Identity=59.4594594594595, Blast_Score=181, Evalue=6e-46,
Organism=Caenorhabditis elegans, GI17556456, Length=439, Percent_Identity=34.1685649202733, Blast_Score=177, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI25141371, Length=352, Percent_Identity=32.3863636363636, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17565438, Length=417, Percent_Identity=25.6594724220624, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17506081, Length=293, Percent_Identity=23.8907849829352, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI32566299, Length=64, Percent_Identity=53.125, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17557151, Length=255, Percent_Identity=28.2352941176471, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6325337, Length=436, Percent_Identity=52.9816513761468, Blast_Score=488, Evalue=1e-138,
Organism=Saccharomyces cerevisiae, GI6319594, Length=436, Percent_Identity=52.9816513761468, Blast_Score=488, Evalue=1e-138,
Organism=Saccharomyces cerevisiae, GI6320377, Length=426, Percent_Identity=36.8544600938967, Blast_Score=270, Evalue=3e-73,
Organism=Saccharomyces cerevisiae, GI6322937, Length=448, Percent_Identity=32.3660714285714, Blast_Score=219, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6324761, Length=425, Percent_Identity=31.7647058823529, Blast_Score=190, Evalue=3e-49,
Organism=Saccharomyces cerevisiae, GI6323320, Length=312, Percent_Identity=29.4871794871795, Blast_Score=86, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6324650, Length=242, Percent_Identity=25.2066115702479, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24652838, Length=446, Percent_Identity=53.8116591928251, Blast_Score=487, Evalue=1e-138,
Organism=Drosophila melanogaster, GI17137572, Length=446, Percent_Identity=53.8116591928251, Blast_Score=487, Evalue=1e-138,
Organism=Drosophila melanogaster, GI45553807, Length=445, Percent_Identity=52.5842696629213, Blast_Score=475, Evalue=1e-134,
Organism=Drosophila melanogaster, GI45553816, Length=445, Percent_Identity=52.5842696629213, Blast_Score=475, Evalue=1e-134,
Organism=Drosophila melanogaster, GI24651721, Length=445, Percent_Identity=52.5842696629213, Blast_Score=475, Evalue=1e-134,
Organism=Drosophila melanogaster, GI17864154, Length=445, Percent_Identity=52.5842696629213, Blast_Score=475, Evalue=1e-134,
Organism=Drosophila melanogaster, GI45550900, Length=432, Percent_Identity=36.1111111111111, Blast_Score=298, Evalue=4e-81,
Organism=Drosophila melanogaster, GI17137380, Length=426, Percent_Identity=36.150234741784, Blast_Score=276, Evalue=2e-74,
Organism=Drosophila melanogaster, GI281363316, Length=426, Percent_Identity=34.037558685446, Blast_Score=199, Evalue=2e-51,
Organism=Drosophila melanogaster, GI17864358, Length=426, Percent_Identity=34.037558685446, Blast_Score=199, Evalue=2e-51,
Organism=Drosophila melanogaster, GI19921738, Length=430, Percent_Identity=29.3023255813954, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI19922512, Length=421, Percent_Identity=26.8408551068884, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24654852, Length=421, Percent_Identity=26.8408551068884, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI19922690, Length=311, Percent_Identity=27.9742765273312, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24647048, Length=371, Percent_Identity=25.3369272237197, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24647046, Length=371, Percent_Identity=25.3369272237197, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI78706572, Length=184, Percent_Identity=32.0652173913043, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): EF1A_METJA (Q57770)

Other databases:

- EMBL:   L77117
- PIR:   D64340
- RefSeq:   NP_247296.1
- ProteinModelPortal:   Q57770
- SMR:   Q57770
- GeneID:   1451178
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0324
- NMPDR:   fig|243232.1.peg.333
- TIGR:   MJ0324
- HOGENOM:   HBG307581
- OMA:   AFIGHVD
- ProtClustDB:   PRK12317
- BioCyc:   MJAN243232:MJ_0324-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00118_A
- InterPro:   IPR000795
- InterPro:   IPR009001
- InterPro:   IPR004539
- InterPro:   IPR004161
- InterPro:   IPR004160
- InterPro:   IPR009000
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00483

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: 3.6.5.3

Molecular weight: Translated: 47280; Mature: 47149

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKQKPVLNVAFIGHVDAGKSTTVGRLLYDSGAIDPQLLEKLKREAQERGKAGFEFAYVM
CCCCCCEEEEEEEEEECCCCCCCHHHEEECCCCCCHHHHHHHHHHHHHHCCCCCCHHHHH
DNLKEERERGVTIDVAHKKFETQKYEVTIVDCPGHRDFIKNMITGASQADAAVLVVDVND
HHHHHHHHCCCEEEEECHHCCCCEEEEEEEECCCCHHHHHHHHCCCCCCCCEEEEEECCC
AKTGIQPQTREHMFLARTLGIKQIAVAINKMDTVNYSQEEYEKMKKMLSEQLLKVLGYNP
CCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
DQIDFIPTASLKGDNVVKRSENMPWYKGPTLVEALDKFQPPEKPTNLPLRIPIQDVYSIT
CCEEEEECCCCCCCCCEECCCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHC
GVGTVPVGRVETGILRPGDKVVFEPAGVSGEVKSIEMHHEQIPQAEPGDNIGFNVRGVSK
CCCCCCCCCCCCCEECCCCEEEEECCCCCCCHHHEEHHHHHCCCCCCCCCCCEEEECCCH
KDIKRGDVCGHPDNPPTVAEEFTAQIVVLQHPTAITVGYTPVFHAHTAQVACTFIELLKK
HHCCCCCCCCCCCCCCHHHHHHCEEEEEEECCCEEEECCCCEEECHHHHHHHHHHHHHHH
LDPRTGQVIEENPQFLKTGDAAIVKIKPTKPMVIENVREIPQLGRFAIRDMGMTIAAGMA
CCCCCCCEECCCCCEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEECCEE
IDVKAKNK
EEEEECCC
>Mature Secondary Structure 
AKQKPVLNVAFIGHVDAGKSTTVGRLLYDSGAIDPQLLEKLKREAQERGKAGFEFAYVM
CCCCCEEEEEEEEEECCCCCCCHHHEEECCCCCCHHHHHHHHHHHHHHCCCCCCHHHHH
DNLKEERERGVTIDVAHKKFETQKYEVTIVDCPGHRDFIKNMITGASQADAAVLVVDVND
HHHHHHHHCCCEEEEECHHCCCCEEEEEEEECCCCHHHHHHHHCCCCCCCCEEEEEECCC
AKTGIQPQTREHMFLARTLGIKQIAVAINKMDTVNYSQEEYEKMKKMLSEQLLKVLGYNP
CCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
DQIDFIPTASLKGDNVVKRSENMPWYKGPTLVEALDKFQPPEKPTNLPLRIPIQDVYSIT
CCEEEEECCCCCCCCCEECCCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHC
GVGTVPVGRVETGILRPGDKVVFEPAGVSGEVKSIEMHHEQIPQAEPGDNIGFNVRGVSK
CCCCCCCCCCCCCEECCCCEEEEECCCCCCCHHHEEHHHHHCCCCCCCCCCCEEEECCCH
KDIKRGDVCGHPDNPPTVAEEFTAQIVVLQHPTAITVGYTPVFHAHTAQVACTFIELLKK
HHCCCCCCCCCCCCCCHHHHHHCEEEEEEECCCEEEECCCCEEECHHHHHHHHHHHHHHH
LDPRTGQVIEENPQFLKTGDAAIVKIKPTKPMVIENVREIPQLGRFAIRDMGMTIAAGMA
CCCCCCCEECCCCCEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEECCEE
IDVKAKNK
EEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087