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The map label for this gene is glyS

Identifier: 15668403

GI number: 15668403

Start: 219104

End: 220837

Strand: Direct

Name: glyS

Synonym: MJ0228

Alternate gene names: 15668403

Gene position: 219104-220837 (Clockwise)

Preceding gene: 15668402

Following gene: 15668408

Centisome position: 13.16

GC content: 32.76

Gene sequence:

>1734_bases
ATGGAAAAAGATATTTACGAAAAAATTATGGATTTAGCAAAAAGAAGAGGTTACTTATGGAGTTCATTTGAAATCTATGG
AGGAATTGCAGGATTTGTTGATTACGGACCTTTAGGATGTTTATTAAAAAATAACATCATATCAAAGTTTAGAGAGCAAT
ATATTATTAAAGAAGGATTTTATGAGATTGAAAGCCCAACAGTAACACCTTATGAAGTTTTAAAGGCATCTGGGCACGTT
GATAACTTTACAGACCCAATTGTTGAGTGTAAAAACTGCTTAGAGTCGTTTAGAGCTGACCACTTAATTGAAGAATTTGT
AGATGTAGATACAGAAGGAAAAACATTAAAGGAATTAGATGAATTAATCAGAAAACACAATATAAGATGTCCAAAGTGTG
GAGGAGAGCTTGGAGAGGTTAAGAAATTTAACTTAATGTTTGTCACATCTATAGGTCCGGGAGGAAAGAGAACTGGATAC
ATGAGACCTGAAACAGCACAGGGAATATTTATACAGTTTAGGAGATTAGCCCAATTCTTTAGAAATAAATTGCCTTTTGG
TGTTGTTCAAATTGGTAAAAGTTATAGAAATGAGATTTCCCCAAGGCAGGGAGTTATTAGGTTGAGAGAATTCACCCAGG
CAGAGATTGAATATTTTGTCCATCCAGAGAGAAAGGAGCATGAGAAATTTGATTTAGTTAAAGATGAAGTTGTTCCATTA
TTACCGGCTGAAAGGCAGATGGATGAAAACTTAAGTGATGATGAAAAGGTAATTAAGATAAGTATTGGTGAGGCTGTTGA
GAAAGGAATTATAAGGCATCAAACAATTGCCTACTTTATAGCTCTAACAAAGAGGTTTTTAGAAGCAATTGGAATTGATA
AAGATAAAATAAGATTTAGACAGCACCTCCCAAATGAGATGGCACACTATGCTATTGACTGTTGGGATGCTGAAATATAC
ACAGAGAGATTTGGATGGATTGAGTGTGTTGGGATTGCAGATAGAACAGATTATGACTTAAGAAGCCACTCTGCTCATAG
TGGAGTTGAACTCTCAGTATTTGTTGAGCTTGATGAAGAAAGAGAGATTGAAACTTATGAAATAAATCTAAATTATAAAG
TTGTTGGAAAGATATTTAAGAAAGATACAAAGGCAATTGAGGCATATATAAACAACTTAAGTGAAAAAGAAAAAGAAGAA
TTTGTTAAAAACATTGAAAATGATGGAAAAGTAATAATAAACATTGATGGAAAGGAATTTGAGATTTTAAAGGATTATGT
TGAAATTAAAAAGGTTAAAAAAGTTATTAAAGGAGAGAAAGTTATCCCTCACGTTATAGAGCCATCCTATGGAATTGATA
GAATAACCTACTGTTTATTAGAACACTCTTACAGAGAGGAAGAAGATAGAGTTTATTTAGATTTAAAACCATCAATTGCC
CCTATAAAAGCTTATGTTCTGCCGTTGGTTAATAAAGATGATATGCCAAAAATAGCTAAAGAAATAGAGCAAATGTTGAG
AGAAAATGGTATTATAGCTGAGTATGATGACAGTGGAGCTATTGGAAGGAGATATATGAGAGCTGATGAAATTGGAGTTC
CATTCTGTATAACAGTAGATGGACAAACATTAGAGGATAGAACTGTAACTGTTAGGGAGAGAAATACAAGAGAACAAGTT
AGAGTTAAAATAGATGAGCTCGTTGATTATTTAAAAGAAAGATTGAAAGAATAA

Upstream 100 bases:

>100_bases
AAATTAAATCCCGGAACAACTGCTGATTTAATTGCATCAAGTTTAATGATATTTATATTAGACAGAATAGATAAGAGAGA
GACAATACTTTGGTGAGAGA

Downstream 100 bases:

>100_bases
AAGAATAGCTATTCATTTTCAAATAATTTTTTTATAATCTCATTGTCAATATAGAACTTAAATTCTTTTGCTTTATATAT
TCTTTTAGCTCTACTTCCTT

Product: glycyl-tRNA synthetase

Products: NA

Alternate protein names: Glycine--tRNA ligase; GlyRS

Number of amino acids: Translated: 577; Mature: 577

Protein sequence:

>577_residues
MEKDIYEKIMDLAKRRGYLWSSFEIYGGIAGFVDYGPLGCLLKNNIISKFREQYIIKEGFYEIESPTVTPYEVLKASGHV
DNFTDPIVECKNCLESFRADHLIEEFVDVDTEGKTLKELDELIRKHNIRCPKCGGELGEVKKFNLMFVTSIGPGGKRTGY
MRPETAQGIFIQFRRLAQFFRNKLPFGVVQIGKSYRNEISPRQGVIRLREFTQAEIEYFVHPERKEHEKFDLVKDEVVPL
LPAERQMDENLSDDEKVIKISIGEAVEKGIIRHQTIAYFIALTKRFLEAIGIDKDKIRFRQHLPNEMAHYAIDCWDAEIY
TERFGWIECVGIADRTDYDLRSHSAHSGVELSVFVELDEEREIETYEINLNYKVVGKIFKKDTKAIEAYINNLSEKEKEE
FVKNIENDGKVIINIDGKEFEILKDYVEIKKVKKVIKGEKVIPHVIEPSYGIDRITYCLLEHSYREEEDRVYLDLKPSIA
PIKAYVLPLVNKDDMPKIAKEIEQMLRENGIIAEYDDSGAIGRRYMRADEIGVPFCITVDGQTLEDRTVTVRERNTREQV
RVKIDELVDYLKERLKE

Sequences:

>Translated_577_residues
MEKDIYEKIMDLAKRRGYLWSSFEIYGGIAGFVDYGPLGCLLKNNIISKFREQYIIKEGFYEIESPTVTPYEVLKASGHV
DNFTDPIVECKNCLESFRADHLIEEFVDVDTEGKTLKELDELIRKHNIRCPKCGGELGEVKKFNLMFVTSIGPGGKRTGY
MRPETAQGIFIQFRRLAQFFRNKLPFGVVQIGKSYRNEISPRQGVIRLREFTQAEIEYFVHPERKEHEKFDLVKDEVVPL
LPAERQMDENLSDDEKVIKISIGEAVEKGIIRHQTIAYFIALTKRFLEAIGIDKDKIRFRQHLPNEMAHYAIDCWDAEIY
TERFGWIECVGIADRTDYDLRSHSAHSGVELSVFVELDEEREIETYEINLNYKVVGKIFKKDTKAIEAYINNLSEKEKEE
FVKNIENDGKVIINIDGKEFEILKDYVEIKKVKKVIKGEKVIPHVIEPSYGIDRITYCLLEHSYREEEDRVYLDLKPSIA
PIKAYVLPLVNKDDMPKIAKEIEQMLRENGIIAEYDDSGAIGRRYMRADEIGVPFCITVDGQTLEDRTVTVRERNTREQV
RVKIDELVDYLKERLKE
>Mature_577_residues
MEKDIYEKIMDLAKRRGYLWSSFEIYGGIAGFVDYGPLGCLLKNNIISKFREQYIIKEGFYEIESPTVTPYEVLKASGHV
DNFTDPIVECKNCLESFRADHLIEEFVDVDTEGKTLKELDELIRKHNIRCPKCGGELGEVKKFNLMFVTSIGPGGKRTGY
MRPETAQGIFIQFRRLAQFFRNKLPFGVVQIGKSYRNEISPRQGVIRLREFTQAEIEYFVHPERKEHEKFDLVKDEVVPL
LPAERQMDENLSDDEKVIKISIGEAVEKGIIRHQTIAYFIALTKRFLEAIGIDKDKIRFRQHLPNEMAHYAIDCWDAEIY
TERFGWIECVGIADRTDYDLRSHSAHSGVELSVFVELDEEREIETYEINLNYKVVGKIFKKDTKAIEAYINNLSEKEKEE
FVKNIENDGKVIINIDGKEFEILKDYVEIKKVKKVIKGEKVIPHVIEPSYGIDRITYCLLEHSYREEEDRVYLDLKPSIA
PIKAYVLPLVNKDDMPKIAKEIEQMLRENGIIAEYDDSGAIGRRYMRADEIGVPFCITVDGQTLEDRTVTVRERNTREQV
RVKIDELVDYLKERLKE

Specific function: Catalyzes the attachment of glycine to tRNA(Gly)

COG id: COG0423

COG function: function code J; Glycyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI116805340, Length=588, Percent_Identity=41.8367346938776, Blast_Score=460, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI17554996, Length=591, Percent_Identity=43.3164128595601, Blast_Score=478, Evalue=1e-135,
Organism=Caenorhabditis elegans, GI32564541, Length=591, Percent_Identity=43.3164128595601, Blast_Score=478, Evalue=1e-135,
Organism=Saccharomyces cerevisiae, GI6325338, Length=593, Percent_Identity=41.6526138279933, Blast_Score=473, Evalue=1e-134,
Organism=Saccharomyces cerevisiae, GI6319597, Length=626, Percent_Identity=39.1373801916933, Blast_Score=456, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24664462, Length=580, Percent_Identity=44.3103448275862, Blast_Score=488, Evalue=1e-138,
Organism=Drosophila melanogaster, GI21357965, Length=580, Percent_Identity=44.3103448275862, Blast_Score=488, Evalue=1e-138,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYG_METJA (Q57681)

Other databases:

- EMBL:   L77117
- PIR:   E64328
- RefSeq:   NP_247199.1
- ProteinModelPortal:   Q57681
- SMR:   Q57681
- GeneID:   1451081
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0228
- NMPDR:   fig|243232.1.peg.236
- TIGR:   MJ0228
- HOGENOM:   HBG319978
- OMA:   AYRNEIS
- ProtClustDB:   PRK04173
- BioCyc:   MJAN243232:MJ_0228-MONOMER
- BRENDA:   6.1.1.14
- GO:   GO:0005737
- HAMAP:   MF_00253_A
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR022960
- InterPro:   IPR002315
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR10745
- PRINTS:   PR01043
- TIGRFAMs:   TIGR00389

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.14

Molecular weight: Translated: 67122; Mature: 67122

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: BINDING 98-98 BINDING 164-164

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKDIYEKIMDLAKRRGYLWSSFEIYGGIAGFVDYGPLGCLLKNNIISKFREQYIIKEGF
CCHHHHHHHHHHHHHCCCCEECHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC
YEIESPTVTPYEVLKASGHVDNFTDPIVECKNCLESFRADHLIEEFVDVDTEGKTLKELD
EECCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
ELIRKHNIRCPKCGGELGEVKKFNLMFVTSIGPGGKRTGYMRPETAQGIFIQFRRLAQFF
HHHHHCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
RNKLPFGVVQIGKSYRNEISPRQGVIRLREFTQAEIEYFVHPERKEHEKFDLVKDEVVPL
HHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHCCCC
LPAERQMDENLSDDEKVIKISIGEAVEKGIIRHQTIAYFIALTKRFLEAIGIDKDKIRFR
CCCHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
QHLPNEMAHYAIDCWDAEIYTERFGWIECVGIADRTDYDLRSHSAHSGVELSVFVELDEE
HHCCHHHHHHHHHHCCHHHHHHHCCCEEEEECCCCCCCCHHCCCCCCCCEEEEEEEECCC
REIETYEINLNYKVVGKIFKKDTKAIEAYINNLSEKEKEEFVKNIENDGKVIINIDGKEF
CCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHH
EILKDYVEIKKVKKVIKGEKVIPHVIEPSYGIDRITYCLLEHSYREEEDRVYLDLKPSIA
HHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCC
PIKAYVLPLVNKDDMPKIAKEIEQMLRENGIIAEYDDSGAIGRRYMRADEIGVPFCITVD
HHHHHHEECCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHCCCCEEEEEC
GQTLEDRTVTVRERNTREQVRVKIDELVDYLKERLKE
CCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEKDIYEKIMDLAKRRGYLWSSFEIYGGIAGFVDYGPLGCLLKNNIISKFREQYIIKEGF
CCHHHHHHHHHHHHHCCCCEECHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC
YEIESPTVTPYEVLKASGHVDNFTDPIVECKNCLESFRADHLIEEFVDVDTEGKTLKELD
EECCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
ELIRKHNIRCPKCGGELGEVKKFNLMFVTSIGPGGKRTGYMRPETAQGIFIQFRRLAQFF
HHHHHCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
RNKLPFGVVQIGKSYRNEISPRQGVIRLREFTQAEIEYFVHPERKEHEKFDLVKDEVVPL
HHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHCCCC
LPAERQMDENLSDDEKVIKISIGEAVEKGIIRHQTIAYFIALTKRFLEAIGIDKDKIRFR
CCCHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
QHLPNEMAHYAIDCWDAEIYTERFGWIECVGIADRTDYDLRSHSAHSGVELSVFVELDEE
HHCCHHHHHHHHHHCCHHHHHHHCCCEEEEECCCCCCCCHHCCCCCCCCEEEEEEEECCC
REIETYEINLNYKVVGKIFKKDTKAIEAYINNLSEKEKEEFVKNIENDGKVIINIDGKEF
CCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHH
EILKDYVEIKKVKKVIKGEKVIPHVIEPSYGIDRITYCLLEHSYREEEDRVYLDLKPSIA
HHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCC
PIKAYVLPLVNKDDMPKIAKEIEQMLRENGIIAEYDDSGAIGRRYMRADEIGVPFCITVD
HHHHHHEECCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHCCCCEEEEEC
GQTLEDRTVTVRERNTREQVRVKIDELVDYLKERLKE
CCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087