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The map label for this gene is purF

Identifier: 15668376

GI number: 15668376

Start: 195727

End: 197142

Strand: Direct

Name: purF

Synonym: MJ0204

Alternate gene names: 15668376

Gene position: 195727-197142 (Clockwise)

Preceding gene: 15668375

Following gene: 15668379

Centisome position: 11.76

GC content: 33.83

Gene sequence:

>1416_bases
ATGTGTGGGATATTTGGAATCTACTCTTATGAAAGGTTAAATGTAGCTAAAAAAATTTATTATGGGTTGTTTGCTTTACA
GCATAGAGGGCAGGAAGGGGCTGGAATTGCTACAAGTGATGGGAAAAATATACACTACTATAAAAATATTGGATTAGTTA
CAGATGTTTTTAAAAATGAGACATTACAAAACTTATTTGGCTATATTGGAATTGGACATGTAAGATATTCAACAACGGGA
GGAAAGGCTGTTGAAAACTGTCAGCCGTTTGTAGTTAAAAGTTCATTTGGTAATATAGCTATAGCCCATAATGGAGATTT
AGTAAATTCAGACGAATTAAGAAGAGAATTAGAGATGAAGGGGCATATATTCACTTCTTCAACTGACTCTGAAGTTATAG
CTCAACTTTTGGTTAGAGAATTGTTAAAAACATCTGATAAGATTGAGGCAATAAAAAATACATTAAAAAAGCTCGTTGGA
GCTTATTCACTTTTAATAATGTTTAATGATTCCTTAATTGCAGTAAGAGACCCTTGGGGCTTTAAACCATTGTGTATTGG
AAGAGATGAGAGCAATATCTATATATCATCTGAGGATTGTGCATTAACAACCTTAGATGCTGAATTTGTTAAAGATATTG
AGCCAGGAGAAATTATAGAAATTAAAGACGGGGAAATAATATCTCATAAATTAGATTATGGTGTTTCTGAATACAACCCT
GTGAATGTCGATGTGCCATGTATATACAGAGGAGCGGCAACTTGTATGTTTGAGTATGTGTATTTTGCAAGACCTGACTC
AACAATTGATGGTATTAGCGTCTATAAGGTTAGAAAGAGGATTGGAAAAATTTTGGCTAAAGAACATCCAGTAGATGCTG
ATGTTGTTTCTCCAATCCCCGATTCAGGAGTCACATTTGCCTTAGGATTTTCTGAAGAGTCAGGGATTCCATACTATGAA
GGTTTAATAAAGAACAGATATGTTGGAAGAACTTTTATTCTTCCATCCCAAAATGAGAGAGAATTGGCAGTAAGGTTAAA
ATTAAGTCCAGTAAAAAGTGTATTGGAAGGGAAGAGGGTTGTTTTGGTTGATGATAGTATTGTTAGAGGAACAACATCAA
GAAGAATTGTAAATATGGTTAGAAAAGCTGGAGCTAAGGAGGTGCATTTAAGAATTGGCTGTCCTAAGATTATATCCCCT
TGCTATTATGGTATAGATATGGCTACTAAAAAGGAACTTATTGCCTCAAACAAAACAGAAGAAGAGATAGGGAAAGCTAT
TGGAGTTGATTCTATTGGATATTTATCATTAGAGGGATTAGTTAAAGCTATAGGTAGAAAAGATTTATGTTTAGCTTGTG
TAACTGGAAAATATCCAACTGAAGTTAATTTTGAAAAGATATTGGGGAGAGAATAA

Upstream 100 bases:

>100_bases
AAAATAAATTATCTTTTTAATTTTACTAATTCTTCTTAATTTTCTTCTTTCTTTTTATTTATTTGCTAAAAGTCTAAAAA
ACTAAAAAAGGGTTTTTAAT

Downstream 100 bases:

>100_bases
CTATCTTTTATCCATACAGTCAATATAATATTTATCAATATTGCGTAATATCGTCAGTGCTTCATTATTTATTTTTCTTC
ATACCCTTGAGTCGCTTTCT

Product: amidophosphoribosyltransferase PurF

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MCGIFGIYSYERLNVAKKIYYGLFALQHRGQEGAGIATSDGKNIHYYKNIGLVTDVFKNETLQNLFGYIGIGHVRYSTTG
GKAVENCQPFVVKSSFGNIAIAHNGDLVNSDELRRELEMKGHIFTSSTDSEVIAQLLVRELLKTSDKIEAIKNTLKKLVG
AYSLLIMFNDSLIAVRDPWGFKPLCIGRDESNIYISSEDCALTTLDAEFVKDIEPGEIIEIKDGEIISHKLDYGVSEYNP
VNVDVPCIYRGAATCMFEYVYFARPDSTIDGISVYKVRKRIGKILAKEHPVDADVVSPIPDSGVTFALGFSEESGIPYYE
GLIKNRYVGRTFILPSQNERELAVRLKLSPVKSVLEGKRVVLVDDSIVRGTTSRRIVNMVRKAGAKEVHLRIGCPKIISP
CYYGIDMATKKELIASNKTEEEIGKAIGVDSIGYLSLEGLVKAIGRKDLCLACVTGKYPTEVNFEKILGRE

Sequences:

>Translated_471_residues
MCGIFGIYSYERLNVAKKIYYGLFALQHRGQEGAGIATSDGKNIHYYKNIGLVTDVFKNETLQNLFGYIGIGHVRYSTTG
GKAVENCQPFVVKSSFGNIAIAHNGDLVNSDELRRELEMKGHIFTSSTDSEVIAQLLVRELLKTSDKIEAIKNTLKKLVG
AYSLLIMFNDSLIAVRDPWGFKPLCIGRDESNIYISSEDCALTTLDAEFVKDIEPGEIIEIKDGEIISHKLDYGVSEYNP
VNVDVPCIYRGAATCMFEYVYFARPDSTIDGISVYKVRKRIGKILAKEHPVDADVVSPIPDSGVTFALGFSEESGIPYYE
GLIKNRYVGRTFILPSQNERELAVRLKLSPVKSVLEGKRVVLVDDSIVRGTTSRRIVNMVRKAGAKEVHLRIGCPKIISP
CYYGIDMATKKELIASNKTEEEIGKAIGVDSIGYLSLEGLVKAIGRKDLCLACVTGKYPTEVNFEKILGRE
>Mature_471_residues
MCGIFGIYSYERLNVAKKIYYGLFALQHRGQEGAGIATSDGKNIHYYKNIGLVTDVFKNETLQNLFGYIGIGHVRYSTTG
GKAVENCQPFVVKSSFGNIAIAHNGDLVNSDELRRELEMKGHIFTSSTDSEVIAQLLVRELLKTSDKIEAIKNTLKKLVG
AYSLLIMFNDSLIAVRDPWGFKPLCIGRDESNIYISSEDCALTTLDAEFVKDIEPGEIIEIKDGEIISHKLDYGVSEYNP
VNVDVPCIYRGAATCMFEYVYFARPDSTIDGISVYKVRKRIGKILAKEHPVDADVVSPIPDSGVTFALGFSEESGIPYYE
GLIKNRYVGRTFILPSQNERELAVRLKLSPVKSVLEGKRVVLVDDSIVRGTTSRRIVNMVRKAGAKEVHLRIGCPKIISP
CYYGIDMATKKELIASNKTEEEIGKAIGVDSIGYLSLEGLVKAIGRKDLCLACVTGKYPTEVNFEKILGRE

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain

Homologues:

Organism=Homo sapiens, GI29570798, Length=519, Percent_Identity=42.9672447013487, Blast_Score=386, Evalue=1e-107,
Organism=Homo sapiens, GI4826742, Length=213, Percent_Identity=30.9859154929577, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI205277386, Length=216, Percent_Identity=27.7777777777778, Blast_Score=66, Evalue=9e-11,
Organism=Escherichia coli, GI1788651, Length=466, Percent_Identity=42.0600858369099, Blast_Score=324, Evalue=9e-90,
Organism=Escherichia coli, GI1790167, Length=225, Percent_Identity=33.7777777777778, Blast_Score=107, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17554892, Length=497, Percent_Identity=42.0523138832998, Blast_Score=352, Evalue=2e-97,
Organism=Caenorhabditis elegans, GI17539970, Length=222, Percent_Identity=29.7297297297297, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17532897, Length=212, Percent_Identity=27.3584905660377, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17532899, Length=212, Percent_Identity=27.3584905660377, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323958, Length=466, Percent_Identity=40.1287553648069, Blast_Score=301, Evalue=2e-82,
Organism=Saccharomyces cerevisiae, GI6322745, Length=209, Percent_Identity=32.5358851674641, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323730, Length=209, Percent_Identity=30.1435406698565, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24659598, Length=494, Percent_Identity=42.7125506072874, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI28573187, Length=497, Percent_Identity=41.851106639839, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24659604, Length=473, Percent_Identity=43.5517970401691, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI21357745, Length=186, Percent_Identity=30.1075268817204, Blast_Score=69, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PUR1_METJA (Q57657)

Other databases:

- EMBL:   L77117
- PIR:   E64325
- RefSeq:   NP_247172.1
- ProteinModelPortal:   Q57657
- SMR:   Q57657
- GeneID:   1451053
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0204
- NMPDR:   fig|243232.1.peg.209
- TIGR:   MJ0204
- HOGENOM:   HBG392416
- OMA:   DSMFEDQ
- ProtClustDB:   CLSK876109
- BioCyc:   MJAN243232:MJ_0204-MONOMER
- BRENDA:   2.4.2.14
- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836
- PANTHER:   PTHR11907
- PIRSF:   PIRSF000485
- TIGRFAMs:   TIGR01134

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran

EC number: =2.4.2.14

Molecular weight: Translated: 52188; Mature: 52188

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: PS51278 GATASE_TYPE_2; PS00103 PUR_PYR_PR_TRANSFER; PS00443 GATASE_TYPE_II

Important sites: ACT_SITE 2-2

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIFGIYSYERLNVAKKIYYGLFALQHRGQEGAGIATSDGKNIHYYKNIGLVTDVFKNE
CCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCEEEEEECCCHHHHHHHHH
TLQNLFGYIGIGHVRYSTTGGKAVENCQPFVVKSSFGNIAIAHNGDLVNSDELRRELEMK
HHHHHHHHHEECEEEEECCCCCHHHCCCCEEEEECCCCEEEEECCCCCCHHHHHHHHHHC
GHIFTSSTDSEVIAQLLVRELLKTSDKIEAIKNTLKKLVGAYSLLIMFNDSLIAVRDPWG
CEEEECCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHEEEEEEECCCEEEEECCCC
FKPLCIGRDESNIYISSEDCALTTLDAEFVKDIEPGEIIEIKDGEIISHKLDYGVSEYNP
CCCEEECCCCCEEEEECCCCEEEEECHHHHHCCCCCCEEEECCCCEEEEHHHCCCCCCCC
VNVDVPCIYRGAATCMFEYVYFARPDSTIDGISVYKVRKRIGKILAKEHPVDADVVSPIP
CCCCCCEEEHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHCCCCC
DSGVTFALGFSEESGIPYYEGLIKNRYVGRTFILPSQNERELAVRLKLSPVKSVLEGKRV
CCCCEEEEECCCCCCCCHHHHHHHCCCCCEEEECCCCCCCEEEEEEEEHHHHHHHCCCEE
VLVDDSIVRGTTSRRIVNMVRKAGAKEVHLRIGCPKIISPCYYGIDMATKKELIASNKTE
EEECCCHHCCCHHHHHHHHHHHCCCCEEEEEECCHHHHCHHHCCCCHHHHHHHHHCCCCH
EEIGKAIGVDSIGYLSLEGLVKAIGRKDLCLACVTGKYPTEVNFEKILGRE
HHHHHHCCCCCCCCEEHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHCCCC
>Mature Secondary Structure
MCGIFGIYSYERLNVAKKIYYGLFALQHRGQEGAGIATSDGKNIHYYKNIGLVTDVFKNE
CCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCEEEEEECCCHHHHHHHHH
TLQNLFGYIGIGHVRYSTTGGKAVENCQPFVVKSSFGNIAIAHNGDLVNSDELRRELEMK
HHHHHHHHHEECEEEEECCCCCHHHCCCCEEEEECCCCEEEEECCCCCCHHHHHHHHHHC
GHIFTSSTDSEVIAQLLVRELLKTSDKIEAIKNTLKKLVGAYSLLIMFNDSLIAVRDPWG
CEEEECCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHEEEEEEECCCEEEEECCCC
FKPLCIGRDESNIYISSEDCALTTLDAEFVKDIEPGEIIEIKDGEIISHKLDYGVSEYNP
CCCEEECCCCCEEEEECCCCEEEEECHHHHHCCCCCCEEEECCCCEEEEHHHCCCCCCCC
VNVDVPCIYRGAATCMFEYVYFARPDSTIDGISVYKVRKRIGKILAKEHPVDADVVSPIP
CCCCCCEEEHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHCCCCC
DSGVTFALGFSEESGIPYYEGLIKNRYVGRTFILPSQNERELAVRLKLSPVKSVLEGKRV
CCCCEEEEECCCCCCCCHHHHHHHCCCCCEEEECCCCCCCEEEEEEEEHHHHHHHCCCEE
VLVDDSIVRGTTSRRIVNMVRKAGAKEVHLRIGCPKIISPCYYGIDMATKKELIASNKTE
EEECCCHHCCCHHHHHHHHHHHCCCCEEEEEECCHHHHCHHHCCCCHHHHHHHHHCCCCH
EEIGKAIGVDSIGYLSLEGLVKAIGRKDLCLACVTGKYPTEVNFEKILGRE
HHHHHHCCCCCCCCEEHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087