BacMap

Definition	Methanocaldococcus jannaschii DSM 2661 chromosome, complete genome.
Accession	NC_000909
Length	1,664,970

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The map label for this gene is pykF [C]

Identifier: 15668279

GI number: 15668279

Start: 104305

End: 105648

Strand: Direct

Name: pykF [C]

Synonym: MJ0108

Alternate gene names: 15668279

Gene position: 104305-105648 (Clockwise)

Preceding gene: 15668278

Following gene: 15668282

Centisome position: 6.26

GC content: 30.95

Gene sequence:

>1344_bases
TTGGTGGGAATGATGAGAAAAACTAAAATTTTAGTCACTTTAGGACCATCCTTAGAAAATAAATTAGATAAAGCAATAAA
TTTGATAGATGGAGTTAGATTTAACATGTCTCATGCCACAACAGATTATTGTGAAAAGTTTTTGAATATATTGGAAAAAA
ATAATATCGCCAAAGTTATGGATTTGAAGGGAATAAAAATTAGAATTAAAGAAGTTAAATTAAAAAATAAAATATTAAAA
ATGGGGGAGAAGGTTGTTATTGGAGAGGATATAAAGCTCAACTACAATATAGACACAATTGAAGAAGGGCATTTTATTTT
AATAAACGATGGGAAAATTAAGCTAAGAGTTGTAGAAAAAACTGATAAAATTATCGCAGTTGTAGAAGTTGGGGGAGAGA
TTAAAGAAGGTATGGGAGTTAATCTTCCAGATACAAGGATAGAACTCCCAATAATTGATGAAACTGATTTGAAGAATATA
AAATTCGCTGTAGAAAAGGACTTTGAATATATTGCTTTATCATTTGTTAGGAATAAGGAAGATGTTAAGGAATTAAAAGA
TATTATATCAGAATACAAAGGAGATTGTGAGGTAATATCAAAAATAGAAACTAAGGAGGGATTAAAGAATATAAAAGAGA
TAGCAAGAGAAAGTGATGGAGTGATGGTAGCAAGGGGAGATTTGGGTGTAGAGGTTCCAATAGAAAATATTCCAATTGAA
CAAAAGAATATATTGAGAATAGCTAATAGATATGGAATTTTGTCAATAACAGCCACACAAATATTGGATTCTATGATAAA
CAATCCATTTCCAACAAGAGCTGAGGTTACAGACATAGCTAATGCCATATACGATGGAACTGACTGCTTAATGCTCTCCA
ACGAAACAACTATTGGAAAATACCCAATAGAGGCGATAAAGGTATTAAATAAGGTTGCCAAAGTGGCAGATGAACATTAT
GAGGAGTTTGGTGATAGAGTTTGTTTAGAGGTTGAGAGCATTGATGAAGGTTTAGTATATGCTGTTTATGAGCTATATAA
GAAGCTAAATACTAAGTTAGTTATAACTCCAACATATTCTGGAAGAACTGCTAAGTTAATATCTAAATTAAGGATAAATA
GTAAAATAATAGCTCCAACGCCAAATATAAGAACTTTAAAAAGGTTGAGATTGGTTTGGGGAGTTGAAAGCTGTTTGATG
GAAGAATTTGATGATATGGAGAAAATAATCAACACCTGTAGAGAAATGGCTAAAAAAGAAATTGGAAAGGGGATTTACTT
AATCACATTAGGTCATCCAATAGGTCAAAAGAAAACTAACACTATAAAAGTTGAAAGTATCTAA

Upstream 100 bases:

>100_bases
CCATGCATCTTATTTTGGTAGAGAGTTAGCTAAGGCAGAGATTGCTTTAAGGATAGGTAAAAAATACAATCAGGACTTTG
ATTTGTTCCTATAATGAATT

Downstream 100 bases:

>100_bases
AAATATTTAAGGTTATAAGAGGTCTAAAATTATATCTAACATTTCTTTGCTATTTGCTACAATAATATTCAATCTATCTG
TTGCATTTAGGTCAAATTTC

Product: pyruvate kinase

Products: NA

Alternate protein names: PK

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MVGMMRKTKILVTLGPSLENKLDKAINLIDGVRFNMSHATTDYCEKFLNILEKNNIAKVMDLKGIKIRIKEVKLKNKILK
MGEKVVIGEDIKLNYNIDTIEEGHFILINDGKIKLRVVEKTDKIIAVVEVGGEIKEGMGVNLPDTRIELPIIDETDLKNI
KFAVEKDFEYIALSFVRNKEDVKELKDIISEYKGDCEVISKIETKEGLKNIKEIARESDGVMVARGDLGVEVPIENIPIE
QKNILRIANRYGILSITATQILDSMINNPFPTRAEVTDIANAIYDGTDCLMLSNETTIGKYPIEAIKVLNKVAKVADEHY
EEFGDRVCLEVESIDEGLVYAVYELYKKLNTKLVITPTYSGRTAKLISKLRINSKIIAPTPNIRTLKRLRLVWGVESCLM
EEFDDMEKIINTCREMAKKEIGKGIYLITLGHPIGQKKTNTIKVESI

Sequences:

>Translated_447_residues
MVGMMRKTKILVTLGPSLENKLDKAINLIDGVRFNMSHATTDYCEKFLNILEKNNIAKVMDLKGIKIRIKEVKLKNKILK
MGEKVVIGEDIKLNYNIDTIEEGHFILINDGKIKLRVVEKTDKIIAVVEVGGEIKEGMGVNLPDTRIELPIIDETDLKNI
KFAVEKDFEYIALSFVRNKEDVKELKDIISEYKGDCEVISKIETKEGLKNIKEIARESDGVMVARGDLGVEVPIENIPIE
QKNILRIANRYGILSITATQILDSMINNPFPTRAEVTDIANAIYDGTDCLMLSNETTIGKYPIEAIKVLNKVAKVADEHY
EEFGDRVCLEVESIDEGLVYAVYELYKKLNTKLVITPTYSGRTAKLISKLRINSKIIAPTPNIRTLKRLRLVWGVESCLM
EEFDDMEKIINTCREMAKKEIGKGIYLITLGHPIGQKKTNTIKVESI
>Mature_447_residues
MVGMMRKTKILVTLGPSLENKLDKAINLIDGVRFNMSHATTDYCEKFLNILEKNNIAKVMDLKGIKIRIKEVKLKNKILK
MGEKVVIGEDIKLNYNIDTIEEGHFILINDGKIKLRVVEKTDKIIAVVEVGGEIKEGMGVNLPDTRIELPIIDETDLKNI
KFAVEKDFEYIALSFVRNKEDVKELKDIISEYKGDCEVISKIETKEGLKNIKEIARESDGVMVARGDLGVEVPIENIPIE
QKNILRIANRYGILSITATQILDSMINNPFPTRAEVTDIANAIYDGTDCLMLSNETTIGKYPIEAIKVLNKVAKVADEHY
EEFGDRVCLEVESIDEGLVYAVYELYKKLNTKLVITPTYSGRTAKLISKLRINSKIIAPTPNIRTLKRLRLVWGVESCLM
EEFDDMEKIINTCREMAKKEIGKGIYLITLGHPIGQKKTNTIKVESI

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family

Homologues:

Organism=Homo sapiens, GI33286422, Length=490, Percent_Identity=34.6938775510204, Blast_Score=233, Evalue=3e-61,
Organism=Homo sapiens, GI33286420, Length=490, Percent_Identity=34.6938775510204, Blast_Score=233, Evalue=3e-61,
Organism=Homo sapiens, GI33286418, Length=489, Percent_Identity=33.5378323108384, Blast_Score=230, Evalue=2e-60,
Organism=Homo sapiens, GI10835121, Length=494, Percent_Identity=31.5789473684211, Blast_Score=217, Evalue=2e-56,
Organism=Homo sapiens, GI32967597, Length=494, Percent_Identity=31.5789473684211, Blast_Score=217, Evalue=2e-56,
Organism=Homo sapiens, GI310128732, Length=291, Percent_Identity=33.6769759450172, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI310128730, Length=291, Percent_Identity=33.6769759450172, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI310128736, Length=240, Percent_Identity=31.6666666666667, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI310128734, Length=240, Percent_Identity=31.6666666666667, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI310128738, Length=208, Percent_Identity=32.6923076923077, Blast_Score=87, Evalue=4e-17,
Organism=Escherichia coli, GI1787965, Length=476, Percent_Identity=35.2941176470588, Blast_Score=238, Evalue=4e-64,
Organism=Escherichia coli, GI1788160, Length=429, Percent_Identity=32.8671328671329, Blast_Score=191, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17544584, Length=489, Percent_Identity=31.4928425357873, Blast_Score=214, Evalue=6e-56,
Organism=Caenorhabditis elegans, GI17506829, Length=485, Percent_Identity=31.9587628865979, Blast_Score=206, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI71984413, Length=485, Percent_Identity=31.9587628865979, Blast_Score=206, Evalue=3e-53,
Organism=Caenorhabditis elegans, GI71984406, Length=485, Percent_Identity=31.9587628865979, Blast_Score=206, Evalue=3e-53,
Organism=Caenorhabditis elegans, GI17506831, Length=485, Percent_Identity=31.9587628865979, Blast_Score=205, Evalue=4e-53,
Organism=Saccharomyces cerevisiae, GI6319279, Length=487, Percent_Identity=33.264887063655, Blast_Score=209, Evalue=6e-55,
Organism=Saccharomyces cerevisiae, GI6324923, Length=487, Percent_Identity=29.7741273100616, Blast_Score=195, Evalue=9e-51,
Organism=Drosophila melanogaster, GI24648966, Length=408, Percent_Identity=33.0882352941176, Blast_Score=210, Evalue=2e-54,
Organism=Drosophila melanogaster, GI24648964, Length=496, Percent_Identity=31.4516129032258, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI28571814, Length=496, Percent_Identity=31.4516129032258, Blast_Score=209, Evalue=3e-54,
Organism=Drosophila melanogaster, GI24581235, Length=305, Percent_Identity=36.0655737704918, Blast_Score=190, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24646914, Length=205, Percent_Identity=38.0487804878049, Blast_Score=144, Evalue=1e-34,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): KPYK_METJA (Q57572)

Other databases:

- EMBL:   L77117
- PIR:   D64313
- RefSeq:   NP_247072.1
- ProteinModelPortal:   Q57572
- SMR:   Q57572
- GeneID:   1450949
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0108
- NMPDR:   fig|243232.1.peg.109
- TIGR:   MJ0108
- HOGENOM:   HBG734376
- OMA:   GAENATK
- ProtClustDB:   CLSK876074
- BioCyc:   MJAN243232:MJ_0108-MONOMER
- BRENDA:   2.7.1.40
- GO:   GO:0006096
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806
- Gene3D:   G3DSA:2.40.33.10
- Gene3D:   G3DSA:3.20.20.60
- Gene3D:   G3DSA:3.40.1380.20
- PANTHER:   PTHR11817
- PRINTS:   PR01050
- TIGRFAMs:   TIGR01064

Pfam domain/function: PF00224 PK; PF02887 PK_C; SSF50800 PK_B_barrel_like; SSF52935 Pyruvate_kinase; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.7.1.40

Molecular weight: Translated: 50491; Mature: 50491

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: BINDING 33-33 BINDING 226-226 BINDING 227-227 BINDING 259-259

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGMMRKTKILVTLGPSLENKLDKAINLIDGVRFNMSHATTDYCEKFLNILEKNNIAKVM
CCCCCEEEEEEEEECCCHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHCCCCEEEE
DLKGIKIRIKEVKLKNKILKMGEKVVIGEDIKLNYNIDTIEEGHFILINDGKIKLRVVEK
ECCCCEEEEEHHHHHHHHHHCCCEEEECCCEEEEECCCEECCCCEEEEECCEEEEEEEEC
TDKIIAVVEVGGEIKEGMGVNLPDTRIELPIIDETDLKNIKFAVEKDFEYIALSFVRNKE
CCCEEEEEECCCCHHCCCCCCCCCCEEEEEECCCCCCCCEEEEEECCHHHHHHHHHCCHH
DVKELKDIISEYKGDCEVISKIETKEGLKNIKEIARESDGVMVARGDLGVEVPIENIPIE
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEECCCCCCCCC
QKNILRIANRYGILSITATQILDSMINNPFPTRAEVTDIANAIYDGTDCLMLSNETTIGK
HHHHHHHHHHCCEEEEEHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCC
YPIEAIKVLNKVAKVADEHYEEFGDRVCLEVESIDEGLVYAVYELYKKLNTKLVITPTYS
CCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHCCEEEEEECCC
GRTAKLISKLRINSKIIAPTPNIRTLKRLRLVWGVESCLMEEFDDMEKIINTCREMAKKE
CCHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGKGIYLITLGHPIGQKKTNTIKVESI
HCCCEEEEEECCCCCCCCCCEEEEECC
>Mature Secondary Structure
MVGMMRKTKILVTLGPSLENKLDKAINLIDGVRFNMSHATTDYCEKFLNILEKNNIAKVM
CCCCCEEEEEEEEECCCHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHCCCCEEEE
DLKGIKIRIKEVKLKNKILKMGEKVVIGEDIKLNYNIDTIEEGHFILINDGKIKLRVVEK
ECCCCEEEEEHHHHHHHHHHCCCEEEECCCEEEEECCCEECCCCEEEEECCEEEEEEEEC
TDKIIAVVEVGGEIKEGMGVNLPDTRIELPIIDETDLKNIKFAVEKDFEYIALSFVRNKE
CCCEEEEEECCCCHHCCCCCCCCCCEEEEEECCCCCCCCEEEEEECCHHHHHHHHHCCHH
DVKELKDIISEYKGDCEVISKIETKEGLKNIKEIARESDGVMVARGDLGVEVPIENIPIE
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEECCCCCCCCC
QKNILRIANRYGILSITATQILDSMINNPFPTRAEVTDIANAIYDGTDCLMLSNETTIGK
HHHHHHHHHHCCEEEEEHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCC
YPIEAIKVLNKVAKVADEHYEEFGDRVCLEVESIDEGLVYAVYELYKKLNTKLVITPTYS
CCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHCCEEEEEECCC
GRTAKLISKLRINSKIIAPTPNIRTLKRLRLVWGVESCLMEEFDDMEKIINTCREMAKKE
CCHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGKGIYLITLGHPIGQKKTNTIKVESI
HCCCEEEEEECCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087