Click here to switch to the map view.

The map label for this gene is cysH [C]

Identifier: 15668237

GI number: 15668237

Start: 63654

End: 65096

Strand: Reverse

Name: cysH [C]

Synonym: MJ0066

Alternate gene names: 15668237

Gene position: 65096-63654 (Counterclockwise)

Preceding gene: 15668239

Following gene: 15668236

Centisome position: 3.91

GC content: 31.53

Gene sequence:

>1443_bases
TTGTGGGAAGTGATAATTATGAAGACATACATTGGAAAGATACATTTAAAATGGTGTAAAAATTGTAATGTCCCATTATT
AGGGAGAGTTTGTGAAGTTTGTGGCTCAAAAGCTGAAGAAGTAAAGCTAACTCCACCAGGAGACCCAAGATTGGGATTTC
AGTATGACATGGATTTTATAAATAAAATTTTGGAAGAAGAATTTGGAGCTAAAAATGTATTAAATGGAAAAATTATTTTG
TTAAATAAAATTCCTGGTAATGAGGAGGCTTATGAGATTATAGTTGATGGAGAAGTTAAATATCTGATATATTTTGATGA
AGATAAGGAGAAATGGAAAGTTAAGCTAAAGTTAAATGGAGCAAAGGATTTAATGGAAAAAGGAGCTTACAAAAAAATAA
TTAAAATAAAGAATGATGTTGTAGAATTTTTAAAAAATAGAAAGGGTTCTGTTTTAAGACCTGGAATAGTCGAATTTACG
GATGATATTGAAGAGAAAGATGATGTGATAATAGTTGATGAGAATGACAGAGTTGTTGGTGTTGGATTAGCTGTTGTTTC
CTCCGAAGATATAAAAAACATGGAGAAGGGAAAAGTAGTTAAGGTTAGATTTTTTATTAAAGATAATGAAGATTATAAGC
CTGGAAAGATTTATGATAACTTAGAAGAGGCATTCGATTTAATGGTTAGAGCTAATGAGGGAGTTATAGATAATTATGAA
AGAAATGCTATTGGATTTATAAAAAATACTTATGAAAAAATTAAAAAACCCGTTATGGTTGCATTCTCTGGAGGAAAAGA
TAGCTTAGTTACTTTAATTTTAACATTAAAGGCTTTAGGTAAAGACATAGATGTTGTGTTTATAGACACTGGCTTAGAAT
TTGAGGAAACACTAAAAAACGTTGAAGATGTTGAAAGACACTATGGTATTAAAATAATTAGGCTGAGAGGAGAGAATTTC
TGGGAGAAAGTTAAAGAATACGGCATTCCAGCAAGAGATTATAGATGGTGTTCTGAAATCTGTAAGTTAGAGCCGTTAAA
AAAGTTTATTGAAGAGAATTACGAAGATGATGTTTTGTCCTTTGTTGGGATTAGGAAGTATGAGAGCTTTAATAGAGCTA
CTAAAAAGAGAATTCATAGAAACACTTACATTAAAAAGCAGATAAATGCCCTCCCAATATTCCATTGGAGTTCTCTGCAT
GTTTGGATATATCTGTTGAGAGAGAAAGCTCCATACAACAAACTGTATGAGAAGGGATTTGATAGGATTGGCTGTTTTAT
GTGTCCAGCTATGGAAATGGGAGAGATGAATAAAATAAAAAGAGAATTTCCAAAACTTTGGGAAAAGTGGGAAAATGTTT
TGAGAGAATATGCTGAAAAACATAACTTAGGAGAGGGGTGGATAAAAAAAGGTTTGTGGAGATGGAAACATAAAAGGCAA
TAA

Upstream 100 bases:

>100_bases
TATCTGGAAGAAACTGTGAGAAGTCCAAAATAATCACCCTATATAAATAACTTATCTAAAACTAAAAAGATTTTCTTTAA
TTTGGTATATAAAAGTTTCT

Downstream 100 bases:

>100_bases
ACTTTAAGCTTTATATAATGTTTCTTTATATGGAGGGAAACATTTTATAATTATTTCCTTACAAATAGTGAAATTTATTA
ATTATTTACTTAAAGTGAGA

Product: hypothetical protein

Products: H+; Adenosine 3',5'-bisphosphate; Sulfite; Oxidized thioredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MWEVIIMKTYIGKIHLKWCKNCNVPLLGRVCEVCGSKAEEVKLTPPGDPRLGFQYDMDFINKILEEEFGAKNVLNGKIIL
LNKIPGNEEAYEIIVDGEVKYLIYFDEDKEKWKVKLKLNGAKDLMEKGAYKKIIKIKNDVVEFLKNRKGSVLRPGIVEFT
DDIEEKDDVIIVDENDRVVGVGLAVVSSEDIKNMEKGKVVKVRFFIKDNEDYKPGKIYDNLEEAFDLMVRANEGVIDNYE
RNAIGFIKNTYEKIKKPVMVAFSGGKDSLVTLILTLKALGKDIDVVFIDTGLEFEETLKNVEDVERHYGIKIIRLRGENF
WEKVKEYGIPARDYRWCSEICKLEPLKKFIEENYEDDVLSFVGIRKYESFNRATKKRIHRNTYIKKQINALPIFHWSSLH
VWIYLLREKAPYNKLYEKGFDRIGCFMCPAMEMGEMNKIKREFPKLWEKWENVLREYAEKHNLGEGWIKKGLWRWKHKRQ

Sequences:

>Translated_480_residues
MWEVIIMKTYIGKIHLKWCKNCNVPLLGRVCEVCGSKAEEVKLTPPGDPRLGFQYDMDFINKILEEEFGAKNVLNGKIIL
LNKIPGNEEAYEIIVDGEVKYLIYFDEDKEKWKVKLKLNGAKDLMEKGAYKKIIKIKNDVVEFLKNRKGSVLRPGIVEFT
DDIEEKDDVIIVDENDRVVGVGLAVVSSEDIKNMEKGKVVKVRFFIKDNEDYKPGKIYDNLEEAFDLMVRANEGVIDNYE
RNAIGFIKNTYEKIKKPVMVAFSGGKDSLVTLILTLKALGKDIDVVFIDTGLEFEETLKNVEDVERHYGIKIIRLRGENF
WEKVKEYGIPARDYRWCSEICKLEPLKKFIEENYEDDVLSFVGIRKYESFNRATKKRIHRNTYIKKQINALPIFHWSSLH
VWIYLLREKAPYNKLYEKGFDRIGCFMCPAMEMGEMNKIKREFPKLWEKWENVLREYAEKHNLGEGWIKKGLWRWKHKRQ
>Mature_480_residues
MWEVIIMKTYIGKIHLKWCKNCNVPLLGRVCEVCGSKAEEVKLTPPGDPRLGFQYDMDFINKILEEEFGAKNVLNGKIIL
LNKIPGNEEAYEIIVDGEVKYLIYFDEDKEKWKVKLKLNGAKDLMEKGAYKKIIKIKNDVVEFLKNRKGSVLRPGIVEFT
DDIEEKDDVIIVDENDRVVGVGLAVVSSEDIKNMEKGKVVKVRFFIKDNEDYKPGKIYDNLEEAFDLMVRANEGVIDNYE
RNAIGFIKNTYEKIKKPVMVAFSGGKDSLVTLILTLKALGKDIDVVFIDTGLEFEETLKNVEDVERHYGIKIIRLRGENF
WEKVKEYGIPARDYRWCSEICKLEPLKKFIEENYEDDVLSFVGIRKYESFNRATKKRIHRNTYIKKQINALPIFHWSSLH
VWIYLLREKAPYNKLYEKGFDRIGCFMCPAMEMGEMNKIKREFPKLWEKWENVLREYAEKHNLGEGWIKKGLWRWKHKRQ

Specific function: Reduction Of Activated Sulfate Into Sulfite. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y066_METJA (Q60377)

Other databases:

- EMBL:   L77117
- PIR:   B64308
- RefSeq:   NP_247030.1
- ProteinModelPortal:   Q60377
- SMR:   Q60377
- GeneID:   1450905
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0066
- NMPDR:   fig|243232.1.peg.67
- TIGR:   MJ0066
- HOGENOM:   HBG539098
- OMA:   GQRKYES
- ProtClustDB:   PRK13794
- BioCyc:   MJAN243232:MJ_0066-MONOMER
- InterPro:   IPR002500
- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR014729
- InterPro:   IPR004521
- Gene3D:   G3DSA:3.40.50.620
- SMART:   SM00359
- TIGRFAMs:   TIGR00451

Pfam domain/function: PF01507 PAPS_reduct; PF01472 PUA; SSF88697 PUA-like

EC number: 1.8.4.8 [C]

Molecular weight: Translated: 56371; Mature: 56371

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWEVIIMKTYIGKIHLKWCKNCNVPLLGRVCEVCGSKAEEVKLTPPGDPRLGFQYDMDFI
CCEEEEEEEHHCCEEEEHHCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCEECHHHH
NKILEEEFGAKNVLNGKIILLNKIPGNEEAYEIIVDGEVKYLIYFDEDKEKWKVKLKLNG
HHHHHHHCCCCCCCCCEEEEEECCCCCCCEEEEEECCCEEEEEEECCCCCEEEEEEEECC
AKDLMEKGAYKKIIKIKNDVVEFLKNRKGSVLRPGIVEFTDDIEEKDDVIIVDENDRVVG
HHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCEEEEECCCCEEE
VGLAVVSSEDIKNMEKGKVVKVRFFIKDNEDYKPGKIYDNLEEAFDLMVRANEGVIDNYE
EEEEEECCHHHHCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
RNAIGFIKNTYEKIKKPVMVAFSGGKDSLVTLILTLKALGKDIDVVFIDTGLEFEETLKN
CCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHH
VEDVERHYGIKIIRLRGENFWEKVKEYGIPARDYRWCSEICKLEPLKKFIEENYEDDVLS
HHHHHHHCCEEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
FVGIRKYESFNRATKKRIHRNTYIKKQINALPIFHWSSLHVWIYLLREKAPYNKLYEKGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHCCCCHHHHHHCCH
DRIGCFMCPAMEMGEMNKIKREFPKLWEKWENVLREYAEKHNLGEGWIKKGLWRWKHKRQ
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MWEVIIMKTYIGKIHLKWCKNCNVPLLGRVCEVCGSKAEEVKLTPPGDPRLGFQYDMDFI
CCEEEEEEEHHCCEEEEHHCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCEECHHHH
NKILEEEFGAKNVLNGKIILLNKIPGNEEAYEIIVDGEVKYLIYFDEDKEKWKVKLKLNG
HHHHHHHCCCCCCCCCEEEEEECCCCCCCEEEEEECCCEEEEEEECCCCCEEEEEEEECC
AKDLMEKGAYKKIIKIKNDVVEFLKNRKGSVLRPGIVEFTDDIEEKDDVIIVDENDRVVG
HHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCEEEEECCCCEEE
VGLAVVSSEDIKNMEKGKVVKVRFFIKDNEDYKPGKIYDNLEEAFDLMVRANEGVIDNYE
EEEEEECCHHHHCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
RNAIGFIKNTYEKIKKPVMVAFSGGKDSLVTLILTLKALGKDIDVVFIDTGLEFEETLKN
CCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHH
VEDVERHYGIKIIRLRGENFWEKVKEYGIPARDYRWCSEICKLEPLKKFIEENYEDDVLS
HHHHHHHCCEEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
FVGIRKYESFNRATKKRIHRNTYIKKQINALPIFHWSSLHVWIYLLREKAPYNKLYEKGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHCCCCHHHHHHCCH
DRIGCFMCPAMEMGEMNKIKREFPKLWEKWENVLREYAEKHNLGEGWIKKGLWRWKHKRQ
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 5700 [C]

Specific activity: 99

Km value (mM): 0.01 {5-phosphoadenosine} [C]

Substrates: 3'-Phosphoadenylyl sulfate; Reduced thioredoxin [C]

Specific reaction: 3'-Phosphoadenylyl sulfate + Reduced thioredoxin --> (2) H+ + Adenosine 3',5'-bisphosphate + Sulfite + Oxidized thioredoxin [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: Adenosine3', 5'-bisphosphate; N-Bromosuccinimide; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087