Click here to switch to the map view.

The map label for this gene is leuS

Identifier: 15646154

GI number: 15646154

Start: 1625497

End: 1627917

Strand: Reverse

Name: leuS

Synonym: HP1547

Alternate gene names: 15646154

Gene position: 1627917-1625497 (Counterclockwise)

Preceding gene: 15646155

Following gene: 15646153

Centisome position: 97.6

GC content: 40.19

Gene sequence:

>2421_bases
ATGGATTTTATCAATATAGAAAAAAAATGGCAAGAATTTTGGTGGAAAAATAAGAGTTTTGAGCCTAAAGACGATTTTAA
CCTCCCTAAAAAATACATTCTAAGCATGCTGCCTTATCCTAGTGGGGAAATCCACATGGGGCATGTGCGCAACTACACCA
TTGGCGATGCTTTAGCGCGTTATTATCGTTTGCACCATTATAATGTGTTACACCCTATGGGGTTTGATTCTTTTGGGATG
CCTGCAGAAAATGCAGCCATTAAGCATGGTATCCACCCTAAAACCTGGACTTATGAAAACATTGAAAACATGCAAAAAGA
GTTTGAAGCTTTAGGGTTTTCTTTTTCTAAAAACAGGGAATTTGCCACTTCAGATCCGGATTACACGAAATTTGAACAGC
GATTTTTCATTGATTTGTGGGAAAAGGGGTTAATTTATCGCAAGAAAGCCATGCTCAATTGGTGCCCTAACGACAAGACC
GTTTTAGCTAATGAGCAAGTCATTGATGGGAGGTGTTGGCGTTGCGATACGGAAGTTATTCAAAAAGAACTCTATCAATA
TTATTTGAAGATCACAAATTACGCTGAAGAATTGCTAAAAGACTTAGAGGCTTTAGAAGATCATTGGCCTTCTCAAGTCC
TAATCATGCAAAAAAACTGGATAGGAAAATCTAGCGGGTTGCAATTTGGTTTTAAAATCGCTGATGAGTGCTTGAAAGCT
TGCAATGGCATTCAAGAAATTGAAGTTTTTACCACAAGAGCGGACACCATTTATGGCGTAACTTACATCGCTATCGCCCC
AGAACACCCTTTAGTAGAGCATGCCATTAAGCAAGTGAGTCAAGAAGTTTCAAAGATGATTAAAGCGATTTTAAACACGA
CTCAAAGAGAAAGAGCTTTAGAGAAAAAAGGGGCGTTTTTAGGCATTTACGCTATCCACCCTTTAACGAAGCAAAAAATC
CCTGTTTGGGTGGCTAATTTCGCCCTAGCTAATTATGGTTCTGGGGCGTTAATGGGCGTGCCAGCCTGCGATGAAAGGGA
CTTTGAATTTGCTAATCTGTATCATATCCCTATTAAAGTGATCACTCAAAGCCCTCAAAATTTGCCCCACACTAAAGAAG
AGGTTTTAAAAAATAGCGGGGAGTGGAGCGATCTTTCTAGCTCAGTGGCCAGAGAACAAATCATCGCTTATTTTGAAAAA
GAAAACCTCGGTAAAAGGGTGATCAACTACCGCTTGCAAGATTGGGGAGTGAGCCGTCAAAGGTATTGGGGAGCGCCCAT
TCCTATGATTCATTGCAACCATTGCGGGATTGTGCCTGAAACCCAACTACCAGTAACTTTACCTGAAGACATTGTGATTG
ATGGGGAGGGCAATCCGTTAGAAAAGCATGCGAGTTGGAAATTCACTCAATGCCCTAAATGCCACAAAAACGCTTTAAGA
GAAACAGACACCATGGACACTTTCATCCAATCTAGTTGGTATTTCTTGCGCTACACCACGCCCAAAAATCAGCGTGAAAA
TCAAGCGTTTGATCAAAATTACTTGAAGTATTTCATGCCAGTGGATACTTATATTGGCGGCATTGAACATGCGATTTTGC
ACTTGTTATACGCGCGCTTTTTCACTAAGGCTTTAAGGGATTTGGGCTATCTTCATTTAGATGAGCCTTTTAAACAGCTT
ATCACTCAAGGCATGGTTTTAAAAAATGGCGCTAAGATGAGCAAGTCTAAAGGCAATGTCGTTAGCCCTAAAGAGATACT
TAAAAAATACGGGGCCGATGCGGCGAGGCTTTTTATCCTTTTTGCTGCCCCACCGGCTAAAGAGTTAGAATGGAATGACA
GCGCTTTAGAAGGTGCGCACCGGTTTATCAAGCGCTTATACGACAAAGCGAATGCCATTAACCCTACCACTTCTAAGCCT
GAATTTAAAGAAGTCAGCCTGAATGAAGCGCAAAAACTAGGGCGTAAAAAAGTCTATGAAGCGTTAAAAAAATCGCATGA
AATTTTCAATAAGGCTGAAAGCGCTTACTCGTTTAACACTTTGATCGCAAGTTGCATGGAGGCTTTAAACGCTTTGAGTG
CGCAAAATAATGAGCGGATTTTATGCGAGGGTTATTTTGTGTTGTTGCAAATTTTAGAGCCTATTATCCCACACACCGCA
TGGGAGTTGAGCGAGAGGCTTTTTAAAAGAGAGAATTTCAAGCCTATAGCGATCGATGAAGACGCTTTGATGGAAGACTT
TATGACTTTAGGGCTTACCATTAATGGCAAAAGGCGTGCAGAATTGAAAGTCAATATTAACGCCAGTAAAGAAGAAATTA
TTGTTTTGGCTAAAAAAGAATTAGAGAAATATTTAGAGAATGCGAGCGTTAAAAAAGAAATTTATGTGCCTAATAAGCTT
GTTAATTTTGTTATCGCATGA

Upstream 100 bases:

>100_bases
TTACATGCTCATGATCGGGGCGTTGATTAGCAACGCTTTCAGTCTCATCTTTTTGTTGATTGAAAGCGTTGTAACGAGCG
AAACGGATTAAGGGGTAGTG

Downstream 100 bases:

>100_bases
GGGCTTTACTTTTTTTTATTTTGTTACTTTGGTTCAAGGGTTGTGGGTATAAGCCTATTGCAGCTTACGCTCAAAACGCT
TTAGGCGATAGCGTATACGT

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS [H]

Number of amino acids: Translated: 806; Mature: 806

Protein sequence:

>806_residues
MDFINIEKKWQEFWWKNKSFEPKDDFNLPKKYILSMLPYPSGEIHMGHVRNYTIGDALARYYRLHHYNVLHPMGFDSFGM
PAENAAIKHGIHPKTWTYENIENMQKEFEALGFSFSKNREFATSDPDYTKFEQRFFIDLWEKGLIYRKKAMLNWCPNDKT
VLANEQVIDGRCWRCDTEVIQKELYQYYLKITNYAEELLKDLEALEDHWPSQVLIMQKNWIGKSSGLQFGFKIADECLKA
CNGIQEIEVFTTRADTIYGVTYIAIAPEHPLVEHAIKQVSQEVSKMIKAILNTTQRERALEKKGAFLGIYAIHPLTKQKI
PVWVANFALANYGSGALMGVPACDERDFEFANLYHIPIKVITQSPQNLPHTKEEVLKNSGEWSDLSSSVAREQIIAYFEK
ENLGKRVINYRLQDWGVSRQRYWGAPIPMIHCNHCGIVPETQLPVTLPEDIVIDGEGNPLEKHASWKFTQCPKCHKNALR
ETDTMDTFIQSSWYFLRYTTPKNQRENQAFDQNYLKYFMPVDTYIGGIEHAILHLLYARFFTKALRDLGYLHLDEPFKQL
ITQGMVLKNGAKMSKSKGNVVSPKEILKKYGADAARLFILFAAPPAKELEWNDSALEGAHRFIKRLYDKANAINPTTSKP
EFKEVSLNEAQKLGRKKVYEALKKSHEIFNKAESAYSFNTLIASCMEALNALSAQNNERILCEGYFVLLQILEPIIPHTA
WELSERLFKRENFKPIAIDEDALMEDFMTLGLTINGKRRAELKVNINASKEEIIVLAKKELEKYLENASVKKEIYVPNKL
VNFVIA

Sequences:

>Translated_806_residues
MDFINIEKKWQEFWWKNKSFEPKDDFNLPKKYILSMLPYPSGEIHMGHVRNYTIGDALARYYRLHHYNVLHPMGFDSFGM
PAENAAIKHGIHPKTWTYENIENMQKEFEALGFSFSKNREFATSDPDYTKFEQRFFIDLWEKGLIYRKKAMLNWCPNDKT
VLANEQVIDGRCWRCDTEVIQKELYQYYLKITNYAEELLKDLEALEDHWPSQVLIMQKNWIGKSSGLQFGFKIADECLKA
CNGIQEIEVFTTRADTIYGVTYIAIAPEHPLVEHAIKQVSQEVSKMIKAILNTTQRERALEKKGAFLGIYAIHPLTKQKI
PVWVANFALANYGSGALMGVPACDERDFEFANLYHIPIKVITQSPQNLPHTKEEVLKNSGEWSDLSSSVAREQIIAYFEK
ENLGKRVINYRLQDWGVSRQRYWGAPIPMIHCNHCGIVPETQLPVTLPEDIVIDGEGNPLEKHASWKFTQCPKCHKNALR
ETDTMDTFIQSSWYFLRYTTPKNQRENQAFDQNYLKYFMPVDTYIGGIEHAILHLLYARFFTKALRDLGYLHLDEPFKQL
ITQGMVLKNGAKMSKSKGNVVSPKEILKKYGADAARLFILFAAPPAKELEWNDSALEGAHRFIKRLYDKANAINPTTSKP
EFKEVSLNEAQKLGRKKVYEALKKSHEIFNKAESAYSFNTLIASCMEALNALSAQNNERILCEGYFVLLQILEPIIPHTA
WELSERLFKRENFKPIAIDEDALMEDFMTLGLTINGKRRAELKVNINASKEEIIVLAKKELEKYLENASVKKEIYVPNKL
VNFVIA
>Mature_806_residues
MDFINIEKKWQEFWWKNKSFEPKDDFNLPKKYILSMLPYPSGEIHMGHVRNYTIGDALARYYRLHHYNVLHPMGFDSFGM
PAENAAIKHGIHPKTWTYENIENMQKEFEALGFSFSKNREFATSDPDYTKFEQRFFIDLWEKGLIYRKKAMLNWCPNDKT
VLANEQVIDGRCWRCDTEVIQKELYQYYLKITNYAEELLKDLEALEDHWPSQVLIMQKNWIGKSSGLQFGFKIADECLKA
CNGIQEIEVFTTRADTIYGVTYIAIAPEHPLVEHAIKQVSQEVSKMIKAILNTTQRERALEKKGAFLGIYAIHPLTKQKI
PVWVANFALANYGSGALMGVPACDERDFEFANLYHIPIKVITQSPQNLPHTKEEVLKNSGEWSDLSSSVAREQIIAYFEK
ENLGKRVINYRLQDWGVSRQRYWGAPIPMIHCNHCGIVPETQLPVTLPEDIVIDGEGNPLEKHASWKFTQCPKCHKNALR
ETDTMDTFIQSSWYFLRYTTPKNQRENQAFDQNYLKYFMPVDTYIGGIEHAILHLLYARFFTKALRDLGYLHLDEPFKQL
ITQGMVLKNGAKMSKSKGNVVSPKEILKKYGADAARLFILFAAPPAKELEWNDSALEGAHRFIKRLYDKANAINPTTSKP
EFKEVSLNEAQKLGRKKVYEALKKSHEIFNKAESAYSFNTLIASCMEALNALSAQNNERILCEGYFVLLQILEPIIPHTA
WELSERLFKRENFKPIAIDEDALMEDFMTLGLTINGKRRAELKVNINASKEEIIVLAKKELEKYLENASVKKEIYVPNKL
VNFVIA

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7661872, Length=871, Percent_Identity=33.1802525832377, Blast_Score=452, Evalue=1e-127,
Organism=Escherichia coli, GI1786861, Length=868, Percent_Identity=42.741935483871, Blast_Score=691, Evalue=0.0,
Organism=Escherichia coli, GI1790708, Length=413, Percent_Identity=23.4866828087167, Blast_Score=92, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71997510, Length=730, Percent_Identity=30.2739726027397, Blast_Score=293, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI71997517, Length=730, Percent_Identity=30.2739726027397, Blast_Score=293, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI212645227, Length=273, Percent_Identity=31.1355311355311, Blast_Score=117, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6323414, Length=804, Percent_Identity=38.18407960199, Blast_Score=511, Evalue=1e-145,
Organism=Saccharomyces cerevisiae, GI6321531, Length=871, Percent_Identity=20.4362801377727, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI21355409, Length=668, Percent_Identity=36.0778443113772, Blast_Score=377, Evalue=1e-104,
Organism=Drosophila melanogaster, GI281366294, Length=141, Percent_Identity=29.0780141843972, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002300
- InterPro:   IPR002302
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR009008 [H]

Pfam domain/function: PF00133 tRNA-synt_1 [H]

EC number: =6.1.1.4 [H]

Molecular weight: Translated: 93165; Mature: 93165

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFINIEKKWQEFWWKNKSFEPKDDFNLPKKYILSMLPYPSGEIHMGHVRNYTIGDALAR
CCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEECCCCCCCHHHHHHH
YYRLHHYNVLHPMGFDSFGMPAENAAIKHGIHPKTWTYENIENMQKEFEALGFSFSKNRE
HHHHHHCCEECCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
FATSDPDYTKFEQRFFIDLWEKGLIYRKKAMLNWCPNDKTVLANEQVIDGRCWRCDTEVI
CCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEECCCEECCCCCCCCHHHH
QKELYQYYLKITNYAEELLKDLEALEDHWPSQVLIMQKNWIGKSSGLQFGFKIADECLKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHH
CNGIQEIEVFTTRADTIYGVTYIAIAPEHPLVEHAIKQVSQEVSKMIKAILNTTQRERAL
HCCCEEEEEEEECCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
EKKGAFLGIYAIHPLTKQKIPVWVANFALANYGSGALMGVPACDERDFEFANLYHIPIKV
HHCCCEEEEEEECCCCCCCCCEEEEHHHHHCCCCCCEECCCCCCCCCCCEEEEEEEEEEE
ITQSPQNLPHTKEEVLKNSGEWSDLSSSVAREQIIAYFEKENLGKRVINYRLQDWGVSRQ
EECCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
RYWGAPIPMIHCNHCGIVPETQLPVTLPEDIVIDGEGNPLEKHASWKFTQCPKCHKNALR
HCCCCCCCEEEECCCCCCCCCCCCEECCCCEEECCCCCCCHHHCCCCCCCCCHHHHHHHH
ETDTMDTFIQSSWYFLRYTTPKNQRENQAFDQNYLKYFMPVDTYIGGIEHAILHLLYARF
HHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FTKALRDLGYLHLDEPFKQLITQGMVLKNGAKMSKSKGNVVSPKEILKKYGADAARLFIL
HHHHHHHCCCEECCHHHHHHHHCCEEECCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEE
FAAPPAKELEWNDSALEGAHRFIKRLYDKANAINPTTSKPEFKEVSLNEAQKLGRKKVYE
EECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHH
ALKKSHEIFNKAESAYSFNTLIASCMEALNALSAQNNERILCEGYFVLLQILEPIIPHTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHCCHH
WELSERLFKRENFKPIAIDEDALMEDFMTLGLTINGKRRAELKVNINASKEEIIVLAKKE
HHHHHHHHHHCCCCCEEECHHHHHHHHHHHCEEECCCEEEEEEEEECCCCCCEEEEEHHH
LEKYLENASVKKEIYVPNKLVNFVIA
HHHHHHCCCCCEEEECCHHHHHHHCC
>Mature Secondary Structure
MDFINIEKKWQEFWWKNKSFEPKDDFNLPKKYILSMLPYPSGEIHMGHVRNYTIGDALAR
CCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEECCCCCCCHHHHHHH
YYRLHHYNVLHPMGFDSFGMPAENAAIKHGIHPKTWTYENIENMQKEFEALGFSFSKNRE
HHHHHHCCEECCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
FATSDPDYTKFEQRFFIDLWEKGLIYRKKAMLNWCPNDKTVLANEQVIDGRCWRCDTEVI
CCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEECCCEECCCCCCCCHHHH
QKELYQYYLKITNYAEELLKDLEALEDHWPSQVLIMQKNWIGKSSGLQFGFKIADECLKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHH
CNGIQEIEVFTTRADTIYGVTYIAIAPEHPLVEHAIKQVSQEVSKMIKAILNTTQRERAL
HCCCEEEEEEEECCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
EKKGAFLGIYAIHPLTKQKIPVWVANFALANYGSGALMGVPACDERDFEFANLYHIPIKV
HHCCCEEEEEEECCCCCCCCCEEEEHHHHHCCCCCCEECCCCCCCCCCCEEEEEEEEEEE
ITQSPQNLPHTKEEVLKNSGEWSDLSSSVAREQIIAYFEKENLGKRVINYRLQDWGVSRQ
EECCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
RYWGAPIPMIHCNHCGIVPETQLPVTLPEDIVIDGEGNPLEKHASWKFTQCPKCHKNALR
HCCCCCCCEEEECCCCCCCCCCCCEECCCCEEECCCCCCCHHHCCCCCCCCCHHHHHHHH
ETDTMDTFIQSSWYFLRYTTPKNQRENQAFDQNYLKYFMPVDTYIGGIEHAILHLLYARF
HHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FTKALRDLGYLHLDEPFKQLITQGMVLKNGAKMSKSKGNVVSPKEILKKYGADAARLFIL
HHHHHHHCCCEECCHHHHHHHHCCEEECCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEE
FAAPPAKELEWNDSALEGAHRFIKRLYDKANAINPTTSKPEFKEVSLNEAQKLGRKKVYE
EECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHH
ALKKSHEIFNKAESAYSFNTLIASCMEALNALSAQNNERILCEGYFVLLQILEPIIPHTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHCCHH
WELSERLFKRENFKPIAIDEDALMEDFMTLGLTINGKRRAELKVNINASKEEIIVLAKKE
HHHHHHHHHHCCCCCEEECHHHHHHHHHHHCEEECCCEEEEEEEEECCCCCCEEEEEHHH
LEKYLENASVKKEIYVPNKLVNFVIA
HHHHHHCCCCCEEEECCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA