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Identifier: 15646008

GI number: 15646008

Start: 1458582

End: 1459724

Strand: Reverse

Name: Not Available

Synonym: HP1398

Alternate gene names: 15646008

Gene position: 1459724-1458582 (Counterclockwise)

Preceding gene: 15646011

Following gene: 15646007

Centisome position: 87.52

GC content: 44.53

Gene sequence:

>1143_bases
ATGACTATTGGGCTAGTTAAAGAAAGCATGGATTTAGAATCCCGAGTGGCTTTAGTGCCTGATGATGTGGCACTAATCGT
TCAAAAGGGCGTGGAGGTTTTAGTTCAAAATAGCGCCGGCGCTAATAGCGGTTATAGCAACGAAGCGTATGAGAGCGTTG
GGGCTAAAATCGTGGATTCTAAAACGGCGTGGGGGCAGGATTTGGTGGTCAAATGCAAAGAGCCTTTAGAGCATGAATAC
CCTTTGCTAAAAGAAAAAGCCACGCTGTTTAGTTACTTGGATTTAGCGTATCAAAAAAGCTTGTGCGAAATGTTTATAGA
CAAAAAAATCACTTCTATTTGCACTGAAACCATTGCCGGGCCTAAAAATGATTACCCTATTTTAGCGCCTATGAGCGTGG
TGGCTGGGAGGTTAGCCGCGCATTTAGTCCAGCATTATTTACTGGCTTTAGAGCATGTTAAGGGGTTTATGGGTAAGGGG
GTCATGCTAGGGGGTTTATCGGGTGCGCAAAGGGCTAAAATTGTCGTAGTTGGGGGCGGTGTGGTTGGCATGGAGAGCGC
GAAAGTCTTAAGCCAAATGGGGGCTAAAGTAACGATTTTAGAATTAGACTACGCTAAATTGCAAAACCACCCTTATTATC
ATTTGTATGATTTAGAAGTTTTAAGCGTGAATGAAGCCAATATCATTCAAGCCTTAAACGGGGCGGTGGGTCTAGTGGGA
GCGGTGTTAGTTACAGCGAGCCAAACCCCTAAAGTGATCTTAAGAAAGCATTTAAAATACATGCAAAAACAAGGGGTAGT
CATTGATGTGGCTTGCGATTTAGGGGGGTGCATAGAGACCATACACCAGACAAGCCATTCTAACCCGGTGTATGTGGAAG
AGGATTTGTTGCATTATGGCGTGCCGAACATGCCAGGGATTGTTGCTAAAACGAGCTCTACGGCTTATAGCCATGCGAGC
GTGCCGTATTTGTTGTATTATTTAGAGCATGGCTTGAAGGGTTTTTTAACAGCCAACACTAAAATCGTGGCAAACACCCT
TGGAGGCTTGAGCGCTTATAACGGCTATATCACCCAAGAAGGCATCGCTAAAGCGTTCAATCTGGCGTTCAAATCGCCTT
TAGAGATTTTAAAGGAGCTTTGA

Upstream 100 bases:

>100_bases
GTAAAAGAGTAAAACCATTCAAGTTTTTGATAGAAAAACACCCCAATAGTCTTATTGAGCGAATATAATCATAACTGACA
CTATTATTTAAAGGATTAAC

Downstream 100 bases:

>100_bases
AGCGAGCTTTAAAAAGAGCATGGCGATAAACCATGCGTCTTAATGTGTGGAAATATTTCAAAAAAAAACACTTTTTAATG
TTATAATCTATCCTAAACAA

Product: alanine dehydrogenase (ald)

Products: NA

Alternate protein names: Stage V sporulation protein N [H]

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MTIGLVKESMDLESRVALVPDDVALIVQKGVEVLVQNSAGANSGYSNEAYESVGAKIVDSKTAWGQDLVVKCKEPLEHEY
PLLKEKATLFSYLDLAYQKSLCEMFIDKKITSICTETIAGPKNDYPILAPMSVVAGRLAAHLVQHYLLALEHVKGFMGKG
VMLGGLSGAQRAKIVVVGGGVVGMESAKVLSQMGAKVTILELDYAKLQNHPYYHLYDLEVLSVNEANIIQALNGAVGLVG
AVLVTASQTPKVILRKHLKYMQKQGVVIDVACDLGGCIETIHQTSHSNPVYVEEDLLHYGVPNMPGIVAKTSSTAYSHAS
VPYLLYYLEHGLKGFLTANTKIVANTLGGLSAYNGYITQEGIAKAFNLAFKSPLEILKEL

Sequences:

>Translated_380_residues
MTIGLVKESMDLESRVALVPDDVALIVQKGVEVLVQNSAGANSGYSNEAYESVGAKIVDSKTAWGQDLVVKCKEPLEHEY
PLLKEKATLFSYLDLAYQKSLCEMFIDKKITSICTETIAGPKNDYPILAPMSVVAGRLAAHLVQHYLLALEHVKGFMGKG
VMLGGLSGAQRAKIVVVGGGVVGMESAKVLSQMGAKVTILELDYAKLQNHPYYHLYDLEVLSVNEANIIQALNGAVGLVG
AVLVTASQTPKVILRKHLKYMQKQGVVIDVACDLGGCIETIHQTSHSNPVYVEEDLLHYGVPNMPGIVAKTSSTAYSHAS
VPYLLYYLEHGLKGFLTANTKIVANTLGGLSAYNGYITQEGIAKAFNLAFKSPLEILKEL
>Mature_379_residues
TIGLVKESMDLESRVALVPDDVALIVQKGVEVLVQNSAGANSGYSNEAYESVGAKIVDSKTAWGQDLVVKCKEPLEHEYP
LLKEKATLFSYLDLAYQKSLCEMFIDKKITSICTETIAGPKNDYPILAPMSVVAGRLAAHLVQHYLLALEHVKGFMGKGV
MLGGLSGAQRAKIVVVGGGVVGMESAKVLSQMGAKVTILELDYAKLQNHPYYHLYDLEVLSVNEANIIQALNGAVGLVGA
VLVTASQTPKVILRKHLKYMQKQGVVIDVACDLGGCIETIHQTSHSNPVYVEEDLLHYGVPNMPGIVAKTSSTAYSHASV
PYLLYYLEHGLKGFLTANTKIVANTLGGLSAYNGYITQEGIAKAFNLAFKSPLEILKEL

Specific function: This enzyme is a key factor in the assimilation of L- alanine as an energy source through the tricarboxylic acid cycle during sporulation [H]

COG id: COG0686

COG function: function code E; Alanine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=349, Percent_Identity=27.7936962750716, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI122939153, Length=349, Percent_Identity=27.7936962750716, Blast_Score=110, Evalue=2e-24,
Organism=Escherichia coli, GI1787887, Length=349, Percent_Identity=24.6418338108883, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17550456, Length=355, Percent_Identity=25.6338028169014, Blast_Score=99, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR008141 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.4.1.1 [H]

Molecular weight: Translated: 40997; Mature: 40866

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIGLVKESMDLESRVALVPDDVALIVQKGVEVLVQNSAGANSGYSNEAYESVGAKIVDS
CCCCCCHHHCCCCCCEEECCCHHHHHHHHHHHEEEECCCCCCCCCCCHHHHHHCCEEECC
KTAWGQDLVVKCKEPLEHEYPLLKEKATLFSYLDLAYQKSLCEMFIDKKITSICTETIAG
CCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PKNDYPILAPMSVVAGRLAAHLVQHYLLALEHVKGFMGKGVMLGGLSGAQRAKIVVVGGG
CCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEEECC
VVGMESAKVLSQMGAKVTILELDYAKLQNHPYYHLYDLEVLSVNEANIIQALNGAVGLVG
EECCHHHHHHHHCCCEEEEEEEEHHHHCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHH
AVLVTASQTPKVILRKHLKYMQKQGVVIDVACDLGGCIETIHQTSHSNPVYVEEDLLHYG
HHHHHCCCCHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHCCCCCCEEEEHHHHHCC
VPNMPGIVAKTSSTAYSHASVPYLLYYLEHGLKGFLTANTKIVANTLGGLSAYNGYITQE
CCCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCEEEECCCEEEEHHHCCHHHHCCCCCHH
GIAKAFNLAFKSPLEILKEL
HHHHHHHHHHHCHHHHHHCC
>Mature Secondary Structure 
TIGLVKESMDLESRVALVPDDVALIVQKGVEVLVQNSAGANSGYSNEAYESVGAKIVDS
CCCCCHHHCCCCCCEEECCCHHHHHHHHHHHEEEECCCCCCCCCCCHHHHHHCCEEECC
KTAWGQDLVVKCKEPLEHEYPLLKEKATLFSYLDLAYQKSLCEMFIDKKITSICTETIAG
CCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PKNDYPILAPMSVVAGRLAAHLVQHYLLALEHVKGFMGKGVMLGGLSGAQRAKIVVVGGG
CCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEEECC
VVGMESAKVLSQMGAKVTILELDYAKLQNHPYYHLYDLEVLSVNEANIIQALNGAVGLVG
EECCHHHHHHHHCCCEEEEEEEEHHHHCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHH
AVLVTASQTPKVILRKHLKYMQKQGVVIDVACDLGGCIETIHQTSHSNPVYVEEDLLHYG
HHHHHCCCCHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHCCCCCCEEEEHHHHHCC
VPNMPGIVAKTSSTAYSHASVPYLLYYLEHGLKGFLTANTKIVANTLGGLSAYNGYITQE
CCCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCEEEECCCEEEEHHHCCHHHHCCCCCHH
GIAKAFNLAFKSPLEILKEL
HHHHHHHHHHHCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8226620; 9384377 [H]