Click here to switch to the map view.

The map label for this gene is Not Available

Identifier: 15645979

GI number: 15645979

Start: 1428975

End: 1429757

Strand: Reverse

Name: Not Available

Synonym: HP1367

Alternate gene names: 15645979

Gene position: 1429757-1428975 (Counterclockwise)

Preceding gene: 15645980

Following gene: 15645978

Centisome position: 85.72

GC content: 35.63

Gene sequence:

>783_bases
TTGATTTTGAATAAGATTTATATAGAAGATGTTTTCACATTTTTAGACAAATTAGAAGATAAAAGCGTTGATTTAGCCAT
TATTGATCCCCCCTACAATCTTAAAATTGCTTCATGGGATAGTTTTAAAAATGATGAAGAGTTTTTAACATTTTCTTACG
CTTGGATTGATAAAATGCTGCCCAAACTTAAAGACACAGGGAGTTTTTATATCTTTAATACCCCTTTTAATTGCGCTTTA
TTTTTAGCGTATTTGCACCATAAAAAAGTGCATTTTTTAAATTTTATCACTTGGGTTAAAAAAGATGGGTTTGCCAACGC
CAAAAAGCGTTATAACCACGCGCAAGAAAGCATTTTATTTTATAGCATGCACAAGAAAAACTACACCTTTAATGCCGATG
AGATTCGCATCGCTTATGAATCCGCTGAACGCATCAAACATGCTCAAAGTAAGGGGATTTTAAAAAATAACAAACGCTGG
TTCCCTAACCCTAAGGGCAAATTATGCCTTGATGTGTGGGAAATCACTTCACAAAGGCATGTTGAAAAAGAGAAGGGTAA
AATCCTTAAGCCCAAACACCCCAGCATCAAACCTAAAGCGCTCATTGAACGCATGATAAAAGCTAGCTCTCACAAAAACG
ATTTGATTTTAGATTTGTTTAGCGGCAGTGGCATGACTAGCTTAGTGGCTAAAAGTTTGGAGCGTAATTTTATAGGGTGT
GAGAGCCATGCTGAATACGTGCATGGGAGTTTGGAAATGTTTAGGTATAATGAATGCGAATAA

Upstream 100 bases:

>100_bases
ACAACCTTAAGGGAAGCAAAACTTTTAAAAAGAAATTTTATAGGTTATGAACTCTATGAAAATTATAAGCCCTTGATTGA
GCAAAAACTAGGAAACTTGT

Downstream 100 bases:

>100_bases
AAAAGGATATTTGACATGCCAAAATTAGAAAAAATTTTGCTAGAAATCACACAGCTTGACCCTAGTAAAGAGTGTTTGAA
ATTCTTAGCTAATCGCATAA

Product: type IIS restriction enzyme M1 protein (mod)

Products: NA

Alternate protein names: M.MboII; Adenine-specific methyltransferase MboII; DNA MTase MboIIA [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MILNKIYIEDVFTFLDKLEDKSVDLAIIDPPYNLKIASWDSFKNDEEFLTFSYAWIDKMLPKLKDTGSFYIFNTPFNCAL
FLAYLHHKKVHFLNFITWVKKDGFANAKKRYNHAQESILFYSMHKKNYTFNADEIRIAYESAERIKHAQSKGILKNNKRW
FPNPKGKLCLDVWEITSQRHVEKEKGKILKPKHPSIKPKALIERMIKASSHKNDLILDLFSGSGMTSLVAKSLERNFIGC
ESHAEYVHGSLEMFRYNECE

Sequences:

>Translated_260_residues
MILNKIYIEDVFTFLDKLEDKSVDLAIIDPPYNLKIASWDSFKNDEEFLTFSYAWIDKMLPKLKDTGSFYIFNTPFNCAL
FLAYLHHKKVHFLNFITWVKKDGFANAKKRYNHAQESILFYSMHKKNYTFNADEIRIAYESAERIKHAQSKGILKNNKRW
FPNPKGKLCLDVWEITSQRHVEKEKGKILKPKHPSIKPKALIERMIKASSHKNDLILDLFSGSGMTSLVAKSLERNFIGC
ESHAEYVHGSLEMFRYNECE
>Mature_260_residues
MILNKIYIEDVFTFLDKLEDKSVDLAIIDPPYNLKIASWDSFKNDEEFLTFSYAWIDKMLPKLKDTGSFYIFNTPFNCAL
FLAYLHHKKVHFLNFITWVKKDGFANAKKRYNHAQESILFYSMHKKNYTFNADEIRIAYESAERIKHAQSKGILKNNKRW
FPNPKGKLCLDVWEITSQRHVEKEKGKILKPKHPSIKPKALIERMIKASSHKNDLILDLFSGSGMTSLVAKSLERNFIGC
ESHAEYVHGSLEMFRYNECE

Specific function: This methylase recognizes the double-stranded sequence GAAGA, causes specific methylation on A-5, and protects the DNA from cleavage by the MboII endonuclease. It is not known if the cytosine of the complementary sequence TCTTC is also methylated by this

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=250, Percent_Identity=32, Blast_Score=104, Evalue=7e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 30471; Mature: 30471

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILNKIYIEDVFTFLDKLEDKSVDLAIIDPPYNLKIASWDSFKNDEEFLTFSYAWIDKML
CCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEECCCCCCCCCCEEEEHHHHHHHHH
PKLKDTGSFYIFNTPFNCALFLAYLHHKKVHFLNFITWVKKDGFANAKKRYNHAQESILF
HHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEE
YSMHKKNYTFNADEIRIAYESAERIKHAQSKGILKNNKRWFPNPKGKLCLDVWEITSQRH
EEEECCCCEECCCCEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEHHHHHHHHHH
VEKEKGKILKPKHPSIKPKALIERMIKASSHKNDLILDLFSGSGMTSLVAKSLERNFIGC
HHHHCCCEECCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCC
ESHAEYVHGSLEMFRYNECE
CHHHHHHHCCHHEEECCCCC
>Mature Secondary Structure
MILNKIYIEDVFTFLDKLEDKSVDLAIIDPPYNLKIASWDSFKNDEEFLTFSYAWIDKML
CCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEECCCCCCCCCCEEEEHHHHHHHHH
PKLKDTGSFYIFNTPFNCALFLAYLHHKKVHFLNFITWVKKDGFANAKKRYNHAQESILF
HHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEE
YSMHKKNYTFNADEIRIAYESAERIKHAQSKGILKNNKRWFPNPKGKLCLDVWEITSQRH
EEEECCCCEECCCCEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEHHHHHHHHHH
VEKEKGKILKPKHPSIKPKALIERMIKASSHKNDLILDLFSGSGMTSLVAKSLERNFIGC
HHHHCCCEECCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCC
ESHAEYVHGSLEMFRYNECE
CHHHHHHHCCHHEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2020540 [H]