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Identifier: 15645978

GI number: 15645978

Start: 1427688

End: 1428959

Strand: Reverse

Name: Not Available

Synonym: HP1366

Alternate gene names: 15645978

Gene position: 1428959-1427688 (Counterclockwise)

Preceding gene: 15645979

Following gene: 15645977

Centisome position: 85.68

GC content: 31.29

Gene sequence:

>1272_bases
ATGCCAAAATTAGAAAAAATTTTGCTAGAAATCACACAGCTTGACCCTAGTAAAGAGTGTTTGAAATTCTTAGCTAATCG
CATAAAAAGTTCTGATTATAGGGGCTTACACTTATCCCAACACAATCGTTACGATCAAAATAAAATTAAAACCATTATTC
AAGCTATTTTCAATGAAGTGGGAGGAGATTTTTTACAAATTCGCACCACCGATATGAGCAAACGCCCTAGCAATATTATA
GGCGAAGAGATTTATGCAAAAGTGGTTGATAATATCTGCAAGTCTGAAATGCCTCAAGATAATTCAGGAAAAAAGAATCA
AGTAACCCAAGACAGCTTGAGAAAAAATCTTTTTGTGGATATGCATAGAATGGGGCTGATTGAACGCTACAATAAAAATA
AGGAACCTACAAACCCCTACATTCAAAGCAATATTAAATATATCAGTTTGACTCCCTTAGCTATAGAATTTTTAAACGCG
CAAGATTTGTTAAGAAAAAATTTTTGTTACACGCAAGCTTTAGAAAACCTTTTGCAAGGTTTTGGAGCAGAATGCAGAGA
GGTAATGATAGAGCTTGACAATCATTATTTAGACATTGAAGAAATGATGTTTTTTGTTACATTTTTAAATATTGAAAATT
TTACTAGAAGTGAAATTATAGAATATGTTAGAGGATATAGGAGTTTAAGCCGCATCCAAAAAGAAAAATTAAAAGAGTTA
GTGCAAGATTATTGCAACCCTAACCATTTTAATGGGAATAAGCTAGAAAAGAGAGATTATCATAATTGGAAAAATCAAGC
CCAACAAATTTTTAGCTTGCTAGAACAAAGCGTGTTTTTTGAAACCAATAAAGAGAGGCTTATTTTAAAAGCGCTCAATG
AAGAAAACAAACAAAACGATAAAAAACTCAAACGCTCTATTAAAGAGAAAGCCCTTTATTTTGAAAAACATGGCGTTAAA
AAAGAAAAGGGCTTTGAATTGCACCACATTGTGCCTTTATGCTTGGCTCGCTCTATAGAAGAATTTGATCTTTTGGATAA
ATGGGAAAACTTAATCTATATTGACGCTTTTAACCATGCGAAAATATCTCAAACGCAAAATAAACATATTTGTTTGTATT
TTAAAAATTGCGATGTGGTTTTATCTAAAGGCTTAAAAGAAGAACAAGAAAGCCTTTATCTTACTTACATTGAAAATGTG
TTATATAAACTTGATTTACAAAATGCCATGTTAAAATACAATAAAGATTTATTGCATTCTAAAAACGGCTGA

Upstream 100 bases:

>100_bases
GGAGCGTAATTTTATAGGGTGTGAGAGCCATGCTGAATACGTGCATGGGAGTTTGGAAATGTTTAGGTATAATGAATGCG
AATAAAAAAGGATATTTGAC

Downstream 100 bases:

>100_bases
TAAAATAAAGCGGTGTCTTTTTTAAGGGAGTGTTTTTTTATAACCGCTTTTAGTATAAAGGCAATTAGCGCTACAATACC
CCCATGCAAAAAAAGATTTT

Product: type IIS restriction enzyme R protein (MBOIIR)

Products: NA

Alternate protein names: R.MboII; Endonuclease MboII; Type II restriction enzyme MboII; Type IIS restriction enzyme MboII [H]

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MPKLEKILLEITQLDPSKECLKFLANRIKSSDYRGLHLSQHNRYDQNKIKTIIQAIFNEVGGDFLQIRTTDMSKRPSNII
GEEIYAKVVDNICKSEMPQDNSGKKNQVTQDSLRKNLFVDMHRMGLIERYNKNKEPTNPYIQSNIKYISLTPLAIEFLNA
QDLLRKNFCYTQALENLLQGFGAECREVMIELDNHYLDIEEMMFFVTFLNIENFTRSEIIEYVRGYRSLSRIQKEKLKEL
VQDYCNPNHFNGNKLEKRDYHNWKNQAQQIFSLLEQSVFFETNKERLILKALNEENKQNDKKLKRSIKEKALYFEKHGVK
KEKGFELHHIVPLCLARSIEEFDLLDKWENLIYIDAFNHAKISQTQNKHICLYFKNCDVVLSKGLKEEQESLYLTYIENV
LYKLDLQNAMLKYNKDLLHSKNG

Sequences:

>Translated_423_residues
MPKLEKILLEITQLDPSKECLKFLANRIKSSDYRGLHLSQHNRYDQNKIKTIIQAIFNEVGGDFLQIRTTDMSKRPSNII
GEEIYAKVVDNICKSEMPQDNSGKKNQVTQDSLRKNLFVDMHRMGLIERYNKNKEPTNPYIQSNIKYISLTPLAIEFLNA
QDLLRKNFCYTQALENLLQGFGAECREVMIELDNHYLDIEEMMFFVTFLNIENFTRSEIIEYVRGYRSLSRIQKEKLKEL
VQDYCNPNHFNGNKLEKRDYHNWKNQAQQIFSLLEQSVFFETNKERLILKALNEENKQNDKKLKRSIKEKALYFEKHGVK
KEKGFELHHIVPLCLARSIEEFDLLDKWENLIYIDAFNHAKISQTQNKHICLYFKNCDVVLSKGLKEEQESLYLTYIENV
LYKLDLQNAMLKYNKDLLHSKNG
>Mature_422_residues
PKLEKILLEITQLDPSKECLKFLANRIKSSDYRGLHLSQHNRYDQNKIKTIIQAIFNEVGGDFLQIRTTDMSKRPSNIIG
EEIYAKVVDNICKSEMPQDNSGKKNQVTQDSLRKNLFVDMHRMGLIERYNKNKEPTNPYIQSNIKYISLTPLAIEFLNAQ
DLLRKNFCYTQALENLLQGFGAECREVMIELDNHYLDIEEMMFFVTFLNIENFTRSEIIEYVRGYRSLSRIQKEKLKELV
QDYCNPNHFNGNKLEKRDYHNWKNQAQQIFSLLEQSVFFETNKERLILKALNEENKQNDKKLKRSIKEKALYFEKHGVKK
EKGFELHHIVPLCLARSIEEFDLLDKWENLIYIDAFNHAKISQTQNKHICLYFKNCDVVLSKGLKEEQESLYLTYIENVL
YKLDLQNAMLKYNKDLLHSKNG

Specific function: Recognizes the double-stranded sequences GAAGA and TCTTC and cleaves respectively 13 bases after G-1 and 7 bases before T- 1, leaving a single 3' protruding nucleotide [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =3.1.21.4 [H]

Molecular weight: Translated: 50048; Mature: 49917

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKLEKILLEITQLDPSKECLKFLANRIKSSDYRGLHLSQHNRYDQNKIKTIIQAIFNEV
CCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHH
GGDFLQIRTTDMSKRPSNIIGEEIYAKVVDNICKSEMPQDNSGKKNQVTQDSLRKNLFVD
CCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
MHRMGLIERYNKNKEPTNPYIQSNIKYISLTPLAIEFLNAQDLLRKNFCYTQALENLLQG
HHHHHHHHHHCCCCCCCCCHHHCCCCEEEECHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
FGAECREVMIELDNHYLDIEEMMFFVTFLNIENFTRSEIIEYVRGYRSLSRIQKEKLKEL
HCHHHHHHHHHHCCCEECHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VQDYCNPNHFNGNKLEKRDYHNWKNQAQQIFSLLEQSVFFETNKERLILKALNEENKQND
HHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHH
KKLKRSIKEKALYFEKHGVKKEKGFELHHIVPLCLARSIEEFDLLDKWENLIYIDAFNHA
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCC
KISQTQNKHICLYFKNCDVVLSKGLKEEQESLYLTYIENVLYKLDLQNAMLKYNKDLLHS
HHCCCCCCEEEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCC
KNG
CCC
>Mature Secondary Structure 
PKLEKILLEITQLDPSKECLKFLANRIKSSDYRGLHLSQHNRYDQNKIKTIIQAIFNEV
CHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHH
GGDFLQIRTTDMSKRPSNIIGEEIYAKVVDNICKSEMPQDNSGKKNQVTQDSLRKNLFVD
CCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
MHRMGLIERYNKNKEPTNPYIQSNIKYISLTPLAIEFLNAQDLLRKNFCYTQALENLLQG
HHHHHHHHHHCCCCCCCCCHHHCCCCEEEECHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
FGAECREVMIELDNHYLDIEEMMFFVTFLNIENFTRSEIIEYVRGYRSLSRIQKEKLKEL
HCHHHHHHHHHHCCCEECHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VQDYCNPNHFNGNKLEKRDYHNWKNQAQQIFSLLEQSVFFETNKERLILKALNEENKQND
HHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHH
KKLKRSIKEKALYFEKHGVKKEKGFELHHIVPLCLARSIEEFDLLDKWENLIYIDAFNHA
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCC
KISQTQNKHICLYFKNCDVVLSKGLKEEQESLYLTYIENVLYKLDLQNAMLKYNKDLLHS
HHCCCCCCEEEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCC
KNG
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2020540 [H]