Click here to switch to the map view.

The map label for this gene is trpA

Identifier: 15645891

GI number: 15645891

Start: 1352363

End: 1353151

Strand: Reverse

Name: trpA

Synonym: HP1277

Alternate gene names: 15645891

Gene position: 1353151-1352363 (Counterclockwise)

Preceding gene: 15645892

Following gene: 15645868

Centisome position: 81.13

GC content: 39.16

Gene sequence:

>789_bases
ATGAGGTATCAAAACATGTTTGAAACCTTAAAAAAACACGAAAAAATGGCGTTTATCCCGTTTGTAACCTTGGGCGATCC
TAATTATGAATTGAGTTTTGAAATCATTAAAACCCTAATTATTAGCGGGGTGAGCGCTTTAGAATTGGGTCTTGCTTTTT
CTGATCCTGTGGCGGATGGCATTACCATACAAGCGAGCCATTTAAGGGCGTTAAAACACGCTAGCATGGCTAAAAATTTC
CAGCTTTTAAAAAAGATTAGAGATTACAACCACAATATTCCCATAGGGCTTTTAGCGTATGCGAATTTAATTTTTTCTTA
TGGCGTTGATGGCTTTTACGCTCAAGCTAAAGAATGCGGTATAGATAGCGTTTTAATAGCGGACATGCCCCTAATAGAAA
AAGAATTAGTCATCAAATCCGCTCAAAAACACCAAATCAAGCAAATCTTTATCGCCAGCCCCAATGCGAGCAGTAAAGAT
TTAGAACAAGTCGCTACGCATTCGCAAGGCTATATCTACGCTTTAGCCAGGAGTGGGGTTACAGGGGCGAGCCGTATTTT
AGAGAATGATTCGAGTGCTATTATTAAAACCTTAAAAGCTTTTAGCCCTACCCCAGCCTTACTGGGCTTTGGCATTTCCA
AAAAAGAACACATCACAAACGCTAAAGGCATGGGTGCTGATGGCGTGATTTGCGGATCAGCGTTAGTCAAAATCATAGAA
GAAAATTTAAACAATGAAAACGCCATGCTGGAAAAAATTAAAGGGTTTATAGGAGGAATGATTTTTTAA

Upstream 100 bases:

>100_bases
CTCAAAAATGCGAAGAAGAAAGCATCATCGTAGTGAATTTAAGCGGCAGAGGGGATAAGGATTTAAGCACCGTTTATAAC
GCTTTAAAAGGAGGTTTAAA

Downstream 100 bases:

>100_bases
GGCTTTTAGGCTTTGTTGCGTTAAAAATTAAAGATCACAGATTAACAATCATCATCAGGTTTTATTGCATGCGCGTTATT
ATTGGGTGTATTTTTATAAA

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MRYQNMFETLKKHEKMAFIPFVTLGDPNYELSFEIIKTLIISGVSALELGLAFSDPVADGITIQASHLRALKHASMAKNF
QLLKKIRDYNHNIPIGLLAYANLIFSYGVDGFYAQAKECGIDSVLIADMPLIEKELVIKSAQKHQIKQIFIASPNASSKD
LEQVATHSQGYIYALARSGVTGASRILENDSSAIIKTLKAFSPTPALLGFGISKKEHITNAKGMGADGVICGSALVKIIE
ENLNNENAMLEKIKGFIGGMIF

Sequences:

>Translated_262_residues
MRYQNMFETLKKHEKMAFIPFVTLGDPNYELSFEIIKTLIISGVSALELGLAFSDPVADGITIQASHLRALKHASMAKNF
QLLKKIRDYNHNIPIGLLAYANLIFSYGVDGFYAQAKECGIDSVLIADMPLIEKELVIKSAQKHQIKQIFIASPNASSKD
LEQVATHSQGYIYALARSGVTGASRILENDSSAIIKTLKAFSPTPALLGFGISKKEHITNAKGMGADGVICGSALVKIIE
ENLNNENAMLEKIKGFIGGMIF
>Mature_262_residues
MRYQNMFETLKKHEKMAFIPFVTLGDPNYELSFEIIKTLIISGVSALELGLAFSDPVADGITIQASHLRALKHASMAKNF
QLLKKIRDYNHNIPIGLLAYANLIFSYGVDGFYAQAKECGIDSVLIADMPLIEKELVIKSAQKHQIKQIFIASPNASSKD
LEQVATHSQGYIYALARSGVTGASRILENDSSAIIKTLKAFSPTPALLGFGISKKEHITNAKGMGADGVICGSALVKIIE
ENLNNENAMLEKIKGFIGGMIF

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate [H]

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family [H]

Homologues:

Organism=Escherichia coli, GI1787514, Length=260, Percent_Identity=46.5384615384615, Blast_Score=226, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6321412, Length=237, Percent_Identity=30.379746835443, Blast_Score=126, Evalue=3e-30,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028 [H]

Pfam domain/function: PF00290 Trp_syntA [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 28595; Mature: 28595

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYQNMFETLKKHEKMAFIPFVTLGDPNYELSFEIIKTLIISGVSALELGLAFSDPVADG
CCHHHHHHHHHHHCCEEEEEEEEECCCCCCHHHHHHHHHHHCCHHHHHHCCCCCCCCCCC
ITIQASHLRALKHASMAKNFQLLKKIRDYNHNIPIGLLAYANLIFSYGVDGFYAQAKECG
EEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCC
IDSVLIADMPLIEKELVIKSAQKHQIKQIFIASPNASSKDLEQVATHSQGYIYALARSGV
CCEEEECCCCHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCC
TGASRILENDSSAIIKTLKAFSPTPALLGFGISKKEHITNAKGMGADGVICGSALVKIIE
CHHHHHHHCCHHHHHHHHHHCCCCHHHHCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHH
ENLNNENAMLEKIKGFIGGMIF
HHCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRYQNMFETLKKHEKMAFIPFVTLGDPNYELSFEIIKTLIISGVSALELGLAFSDPVADG
CCHHHHHHHHHHHCCEEEEEEEEECCCCCCHHHHHHHHHHHCCHHHHHHCCCCCCCCCCC
ITIQASHLRALKHASMAKNFQLLKKIRDYNHNIPIGLLAYANLIFSYGVDGFYAQAKECG
EEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCC
IDSVLIADMPLIEKELVIKSAQKHQIKQIFIASPNASSKDLEQVATHSQGYIYALARSGV
CCEEEECCCCHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCC
TGASRILENDSSAIIKTLKAFSPTPALLGFGISKKEHITNAKGMGADGVICGSALVKIIE
CHHHHHHHCCHHHHHHHHHHCCCCHHHHCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHH
ENLNNENAMLEKIKGFIGGMIF
HHCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]