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The map label for this gene is alaS

Identifier: 15645855

GI number: 15645855

Start: 1314188

End: 1316731

Strand: Reverse

Name: alaS

Synonym: HP1241

Alternate gene names: 15645855

Gene position: 1316731-1314188 (Counterclockwise)

Preceding gene: 15645857

Following gene: 15645854

Centisome position: 78.95

GC content: 42.53

Gene sequence:

>2544_bases
ATGGATATTCGCAACGAATTTTTACAATTTTTTCAAAATAAAGGGCATGCCGTTTATCCTAGCATGCCTTTAGTGCCTAA
TGACGCTACCTTGCTTTTTACCAATGCCGGCATGGTGCAATTTAAAGATATTTTTACCGGGATTGTGCCACGCCCTAGCA
TTCCTAGAGCGGCAAGCTCGCAATTGTGCATGCGCGCAGGCGGCAAGCATAACGATTTGGAAAATGTCGGTTATACCGCA
AGGCACCACACGCTTTTTGAAATGCTAGGGAATTTCTCTTTTGGGGATTATTTCAAAGAAGAAGCGATCTTGTTTGCGTG
GGAATTTGTAACCAAGAATTTAGGGTTTAAGCCTAAAGATTTATACATCAGCGTGCATGAAAAGGACGATGAAGCCGTTA
AATTATGGGAAAAGTTTGTGCCTGTTGATAGGATTAAAAAAATGGGCGATAAAGATAATTTTTGGCAAATGGGCGATAGC
GGGCCTTGCGGGCCTTGCAGTGAAATTTACATTGATCAGGGCGAAAAACACTTTAAGGGGAGCGAGGATTATTTTGGGGG
CGAGGGCGATAGGTTTTTAGAAATTTGGAATCTGGTGTTCATGCAATACGAACGCTCTAATGATGGCGTTTTATCCCCCT
TGCCAAAGCCTAGCATTGATACAGGCATGGGATTAGAAAGGGTGCAAGCGCTATTAGAACATAAGCTCAATAATTTTGAT
TCTTCATTATTTGCGCCCCTAATGGAAGAAATCAGCGAGCTTACAAGCCTAGATTATGCGAGCGAGTTCCAGCCAAGCTT
TAGGGTAGTGGCCGATCACGCAAGAGCGGTAGCATTCTTGCTCGCTCAAGGGGTGCATTTCAATAAGGAAGGCCGTGGCT
ATGTTTTAAGGCGCATTTTAAGGCGAGCCTTAAGGCATGGGTATTTAATGGGCTTGAAAGAAGCGTTTTTATACAAAGTC
GTGGGCGTGGTGTGCGAGCAATTTGCTAACACGCATGCGTATTTGAAAGAGTCTAAAGAAATGGTGGTAAAAGAATGTTT
TGAAGAAGAAGAGCACTTTTTAGAGACTTTGGAATCGGGCATGGAATTGTTTAACTTGTCTTTAAAGCATTTGAATGAAA
ATAAAATCTTTGATGGCAAGATCGCTTTCAAGCTTTATGACACTTTTGGTTTCCCTTTGGATTTAACAAACGACATGTTA
AGAAGTCATGGGGCGTGTGCGGATATGCAAGGCTTTGAATTGTGCATGCAAGAGCAAGTGAAACGCTCTAAAGCTTCATG
GAAAGGCAAACAAAACAACGCCGATTTTAGCGCTATTTTAAACGCTTATGCACCTAATGTTTTTGTGGGGTATGAAACGA
CAGAATGTTCTGCTAAAGTTTTAGGGTTTTTTGATAGCGATTTTAAAGAAATAACCGATGCAAATCCTAACCAAGAAGTC
TGGGTGTTGTTAGAAAAAACCCCTTTTTATGCAGAAGGTGGAGGGGCTATAGGCGATAGGGGCGCGCTTTTTAAAGACAA
TGGAGAAGTGGCTATCGTGTTAGATACAAAAAACTTTTTTGGGCTTAATTTTTCACTCCTTGAAATCAAAAAAGCGCTAA
AAAAAGGCGATCAAGTGATCGCGCAAGTGAGCGATGAGCGCTTTGAAATCGCCAAACACCATAGTGCGACTCATTTATTG
CAGAGCGCTTTAAGAGAAGTTTTAGGCTCGCATGTGAGTCAAGCGGGGAGTTTAGTGGAATCCAAGCGATTGCGCTTTGA
TTTCTCGCATGCTAAAGCGCTCAATGATGAAGAGCTAGAAAAAGTAGAAGATTTAGTCAACGCTCAAATTTTCAAGCACC
TAAATAGCCAGGTGGAGCATATGCCTTTAAACCAAGCCAAAGATAAGGGAGCGTTAGCGTTATTCAGTGAAAAATACGCT
GAAAATGTGCGGGTGGTGAGCTTTAAAGAAGCGTCCATTGAATTGTGTGGGGGCATTCATGTGGAAAATACTGGGCTTAT
TGGGGGGTTTAGGATTGTAAAAGAAAGCGGGGTGAGTAGTGGGGTCAGACGCATTGAAGCGGTGTGCGGGAAAGCCTTTT
ACCAACTGGCTAAAGAAGAAAATAAAGAGCTTAAAAACGCTAAGACTTTATTGAAAAATAACGATGTGATCGCCGGTATC
AATAAGCTTAAAGAGAGCGTGAAAAACAGCCAAAAAGCCCCCGTTTCTATGGATTTACCGGTTGAAAAAATCCATGGCGT
GAATTTGGTGGTGGGCGTAGTGGAACAAGGCGACATTAAAGAAATGATTGACCGATTGAAAAGTAAGCATGAAAGATTGC
TCGCTATGGTGTTTAAAAAAGAAAATGAGCGAATCACTCTCGCATGCGGGGTGAAAAACGCGCCCATAAAAGCGAATGTG
TGGGCTAATGAAGTGGCGCAAATTTTAGGGGGCAAAGGGGGCGGGAGAGGTGATTTTGCGAGCGCTGGAGGCAAGGATAT
TGAAAATTTGCAAGCCGCACTCAATTTAGCGAAAAATACCGCTCTTAAAGCTTTAGAGGGATAG

Upstream 100 bases:

>100_bases
GGATGTTTAAAGTATAATAAACTATCTTTTTAAAAAAATAACTTAAAAGAGCTATAAAATAGCCTTAAAATACGCGATAA
AACAACAAAAAGGAATACCC

Downstream 100 bases:

>100_bases
CATGGAGCTTATTTTAGGCTCTCAATCCAGCACTAGGGCGAATCTCTTAAAAGAGCATGGGATTAAGTTTGAACAAAAAG
CGCTCTATTTTGATGAAGAA

Product: alanyl-tRNA synthetase

Products: NA

Alternate protein names: Alanine--tRNA ligase; AlaRS [H]

Number of amino acids: Translated: 847; Mature: 847

Protein sequence:

>847_residues
MDIRNEFLQFFQNKGHAVYPSMPLVPNDATLLFTNAGMVQFKDIFTGIVPRPSIPRAASSQLCMRAGGKHNDLENVGYTA
RHHTLFEMLGNFSFGDYFKEEAILFAWEFVTKNLGFKPKDLYISVHEKDDEAVKLWEKFVPVDRIKKMGDKDNFWQMGDS
GPCGPCSEIYIDQGEKHFKGSEDYFGGEGDRFLEIWNLVFMQYERSNDGVLSPLPKPSIDTGMGLERVQALLEHKLNNFD
SSLFAPLMEEISELTSLDYASEFQPSFRVVADHARAVAFLLAQGVHFNKEGRGYVLRRILRRALRHGYLMGLKEAFLYKV
VGVVCEQFANTHAYLKESKEMVVKECFEEEEHFLETLESGMELFNLSLKHLNENKIFDGKIAFKLYDTFGFPLDLTNDML
RSHGACADMQGFELCMQEQVKRSKASWKGKQNNADFSAILNAYAPNVFVGYETTECSAKVLGFFDSDFKEITDANPNQEV
WVLLEKTPFYAEGGGAIGDRGALFKDNGEVAIVLDTKNFFGLNFSLLEIKKALKKGDQVIAQVSDERFEIAKHHSATHLL
QSALREVLGSHVSQAGSLVESKRLRFDFSHAKALNDEELEKVEDLVNAQIFKHLNSQVEHMPLNQAKDKGALALFSEKYA
ENVRVVSFKEASIELCGGIHVENTGLIGGFRIVKESGVSSGVRRIEAVCGKAFYQLAKEENKELKNAKTLLKNNDVIAGI
NKLKESVKNSQKAPVSMDLPVEKIHGVNLVVGVVEQGDIKEMIDRLKSKHERLLAMVFKKENERITLACGVKNAPIKANV
WANEVAQILGGKGGGRGDFASAGGKDIENLQAALNLAKNTALKALEG

Sequences:

>Translated_847_residues
MDIRNEFLQFFQNKGHAVYPSMPLVPNDATLLFTNAGMVQFKDIFTGIVPRPSIPRAASSQLCMRAGGKHNDLENVGYTA
RHHTLFEMLGNFSFGDYFKEEAILFAWEFVTKNLGFKPKDLYISVHEKDDEAVKLWEKFVPVDRIKKMGDKDNFWQMGDS
GPCGPCSEIYIDQGEKHFKGSEDYFGGEGDRFLEIWNLVFMQYERSNDGVLSPLPKPSIDTGMGLERVQALLEHKLNNFD
SSLFAPLMEEISELTSLDYASEFQPSFRVVADHARAVAFLLAQGVHFNKEGRGYVLRRILRRALRHGYLMGLKEAFLYKV
VGVVCEQFANTHAYLKESKEMVVKECFEEEEHFLETLESGMELFNLSLKHLNENKIFDGKIAFKLYDTFGFPLDLTNDML
RSHGACADMQGFELCMQEQVKRSKASWKGKQNNADFSAILNAYAPNVFVGYETTECSAKVLGFFDSDFKEITDANPNQEV
WVLLEKTPFYAEGGGAIGDRGALFKDNGEVAIVLDTKNFFGLNFSLLEIKKALKKGDQVIAQVSDERFEIAKHHSATHLL
QSALREVLGSHVSQAGSLVESKRLRFDFSHAKALNDEELEKVEDLVNAQIFKHLNSQVEHMPLNQAKDKGALALFSEKYA
ENVRVVSFKEASIELCGGIHVENTGLIGGFRIVKESGVSSGVRRIEAVCGKAFYQLAKEENKELKNAKTLLKNNDVIAGI
NKLKESVKNSQKAPVSMDLPVEKIHGVNLVVGVVEQGDIKEMIDRLKSKHERLLAMVFKKENERITLACGVKNAPIKANV
WANEVAQILGGKGGGRGDFASAGGKDIENLQAALNLAKNTALKALEG
>Mature_847_residues
MDIRNEFLQFFQNKGHAVYPSMPLVPNDATLLFTNAGMVQFKDIFTGIVPRPSIPRAASSQLCMRAGGKHNDLENVGYTA
RHHTLFEMLGNFSFGDYFKEEAILFAWEFVTKNLGFKPKDLYISVHEKDDEAVKLWEKFVPVDRIKKMGDKDNFWQMGDS
GPCGPCSEIYIDQGEKHFKGSEDYFGGEGDRFLEIWNLVFMQYERSNDGVLSPLPKPSIDTGMGLERVQALLEHKLNNFD
SSLFAPLMEEISELTSLDYASEFQPSFRVVADHARAVAFLLAQGVHFNKEGRGYVLRRILRRALRHGYLMGLKEAFLYKV
VGVVCEQFANTHAYLKESKEMVVKECFEEEEHFLETLESGMELFNLSLKHLNENKIFDGKIAFKLYDTFGFPLDLTNDML
RSHGACADMQGFELCMQEQVKRSKASWKGKQNNADFSAILNAYAPNVFVGYETTECSAKVLGFFDSDFKEITDANPNQEV
WVLLEKTPFYAEGGGAIGDRGALFKDNGEVAIVLDTKNFFGLNFSLLEIKKALKKGDQVIAQVSDERFEIAKHHSATHLL
QSALREVLGSHVSQAGSLVESKRLRFDFSHAKALNDEELEKVEDLVNAQIFKHLNSQVEHMPLNQAKDKGALALFSEKYA
ENVRVVSFKEASIELCGGIHVENTGLIGGFRIVKESGVSSGVRRIEAVCGKAFYQLAKEENKELKNAKTLLKNNDVIAGI
NKLKESVKNSQKAPVSMDLPVEKIHGVNLVVGVVEQGDIKEMIDRLKSKHERLLAMVFKKENERITLACGVKNAPIKANV
WANEVAQILGGKGGGRGDFASAGGKDIENLQAALNLAKNTALKALEG

Specific function: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction:alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editin

COG id: COG0013

COG function: function code J; Alanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI109148542, Length=963, Percent_Identity=35.7217030114226, Blast_Score=486, Evalue=1e-137,
Organism=Homo sapiens, GI38569417, Length=954, Percent_Identity=32.0754716981132, Blast_Score=405, Evalue=1e-113,
Organism=Escherichia coli, GI1789048, Length=863, Percent_Identity=46.5816917728853, Blast_Score=699, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17506981, Length=968, Percent_Identity=33.4710743801653, Blast_Score=458, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI17536681, Length=737, Percent_Identity=32.5644504748982, Blast_Score=332, Evalue=4e-91,
Organism=Saccharomyces cerevisiae, GI6324911, Length=751, Percent_Identity=40.745672436751, Blast_Score=499, Evalue=1e-142,
Organism=Drosophila melanogaster, GI24582809, Length=965, Percent_Identity=32.7461139896373, Blast_Score=458, Evalue=1e-129,
Organism=Drosophila melanogaster, GI45552267, Length=965, Percent_Identity=32.7461139896373, Blast_Score=458, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24658214, Length=794, Percent_Identity=29.5969773299748, Blast_Score=291, Evalue=1e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002318
- InterPro:   IPR018162
- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR023033
- InterPro:   IPR003156
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF02272 DHHA1; PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: =6.1.1.7 [H]

Molecular weight: Translated: 94701; Mature: 94701

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIRNEFLQFFQNKGHAVYPSMPLVPNDATLLFTNAGMVQFKDIFTGIVPRPSIPRAASS
CCHHHHHHHHHHCCCCEECCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCCCCCCCHHHH
QLCMRAGGKHNDLENVGYTARHHTLFEMLGNFSFGDYFKEEAILFAWEFVTKNLGFKPKD
HHHHHCCCCCCCHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCE
LYISVHEKDDEAVKLWEKFVPVDRIKKMGDKDNFWQMGDSGPCGPCSEIYIDQGEKHFKG
EEEEEECCCHHHHHHHHHHCCHHHHHHCCCCCCCEECCCCCCCCCCCHHHCCCCCHHCCC
SEDYFGGEGDRFLEIWNLVFMQYERSNDGVLSPLPKPSIDTGMGLERVQALLEHKLNNFD
CCCCCCCCCHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
SSLFAPLMEEISELTSLDYASEFQPSFRVVADHARAVAFLLAQGVHFNKEGRGYVLRRIL
HHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
RRALRHGYLMGLKEAFLYKVVGVVCEQFANTHAYLKESKEMVVKECFEEEEHFLETLESG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MELFNLSLKHLNENKIFDGKIAFKLYDTFGFPLDLTNDMLRSHGACADMQGFELCMQEQV
HHHHHHHHHHCCCCCEECCEEEEEEEECCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHH
KRSKASWKGKQNNADFSAILNAYAPNVFVGYETTECSAKVLGFFDSDFKEITDANPNQEV
HHHHHHCCCCCCCCHHHHHHHHHCCCEEEEECCCCCCHHHHHHHCHHHHHHCCCCCCCEE
WVLLEKTPFYAEGGGAIGDRGALFKDNGEVAIVLDTKNFFGLNFSLLEIKKALKKGDQVI
EEEEECCCCEECCCCCCCCCCCEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
AQVSDERFEIAKHHSATHLLQSALREVLGSHVSQAGSLVESKRLRFDFSHAKALNDEELE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECHHHHCCCHHHHH
KVEDLVNAQIFKHLNSQVEHMPLNQAKDKGALALFSEKYAENVRVVSFKEASIELCGGIH
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCHHHHCCEE
VENTGLIGGFRIVKESGVSSGVRRIEAVCGKAFYQLAKEENKELKNAKTLLKNNDVIAGI
ECCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECH
NKLKESVKNSQKAPVSMDLPVEKIHGVNLVVGVVEQGDIKEMIDRLKSKHERLLAMVFKK
HHHHHHHHCCCCCCCEECCCHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHC
ENERITLACGVKNAPIKANVWANEVAQILGGKGGGRGDFASAGGKDIENLQAALNLAKNT
CCCEEEEEECCCCCCEEECCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
ALKALEG
HHHHCCC
>Mature Secondary Structure
MDIRNEFLQFFQNKGHAVYPSMPLVPNDATLLFTNAGMVQFKDIFTGIVPRPSIPRAASS
CCHHHHHHHHHHCCCCEECCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCCCCCCCHHHH
QLCMRAGGKHNDLENVGYTARHHTLFEMLGNFSFGDYFKEEAILFAWEFVTKNLGFKPKD
HHHHHCCCCCCCHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCE
LYISVHEKDDEAVKLWEKFVPVDRIKKMGDKDNFWQMGDSGPCGPCSEIYIDQGEKHFKG
EEEEEECCCHHHHHHHHHHCCHHHHHHCCCCCCCEECCCCCCCCCCCHHHCCCCCHHCCC
SEDYFGGEGDRFLEIWNLVFMQYERSNDGVLSPLPKPSIDTGMGLERVQALLEHKLNNFD
CCCCCCCCCHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
SSLFAPLMEEISELTSLDYASEFQPSFRVVADHARAVAFLLAQGVHFNKEGRGYVLRRIL
HHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
RRALRHGYLMGLKEAFLYKVVGVVCEQFANTHAYLKESKEMVVKECFEEEEHFLETLESG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MELFNLSLKHLNENKIFDGKIAFKLYDTFGFPLDLTNDMLRSHGACADMQGFELCMQEQV
HHHHHHHHHHCCCCCEECCEEEEEEEECCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHH
KRSKASWKGKQNNADFSAILNAYAPNVFVGYETTECSAKVLGFFDSDFKEITDANPNQEV
HHHHHHCCCCCCCCHHHHHHHHHCCCEEEEECCCCCCHHHHHHHCHHHHHHCCCCCCCEE
WVLLEKTPFYAEGGGAIGDRGALFKDNGEVAIVLDTKNFFGLNFSLLEIKKALKKGDQVI
EEEEECCCCEECCCCCCCCCCCEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
AQVSDERFEIAKHHSATHLLQSALREVLGSHVSQAGSLVESKRLRFDFSHAKALNDEELE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECHHHHCCCHHHHH
KVEDLVNAQIFKHLNSQVEHMPLNQAKDKGALALFSEKYAENVRVVSFKEASIELCGGIH
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCHHHHCCEE
VENTGLIGGFRIVKESGVSSGVRRIEAVCGKAFYQLAKEENKELKNAKTLLKNNDVIAGI
ECCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECH
NKLKESVKNSQKAPVSMDLPVEKIHGVNLVVGVVEQGDIKEMIDRLKSKHERLLAMVFKK
HHHHHHHHCCCCCCCEECCCHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHC
ENERITLACGVKNAPIKANVWANEVAQILGGKGGGRGDFASAGGKDIENLQAALNLAKNT
CCCEEEEEECCCCCCEEECCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
ALKALEG
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA