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Identifier: 15645409

GI number: 15645409

Start: 845540

End: 846835

Strand: Reverse

Name: Not Available

Synonym: HP0790

Alternate gene names: 15645409

Gene position: 846835-845540 (Counterclockwise)

Preceding gene: 15645410

Following gene: 15645408

Centisome position: 50.77

GC content: 35.49

Gene sequence:

>1296_bases
ATGCATAAAATAGAGCGCTTACTCCAAACTCTAGCGCCTAAGGGGGTGGAGTTTAAAACGCTTGAAGAGGTTTTTGAAAT
TAAAAATGGTTACACCCCATCAAAAAACAATCCTGAATTTTGGAAAAATGGGACTATCCCTTGGTTTAGAATGGAAGACA
TTAGAGAAAATGGGAGGATTTTAAAAGACTCTATCCAACACATTACCCCAAAGGCTTTAAAGGGTAAGAAATTATTCCCT
AAAAATTCTATTATTATTTCTACGAAAGCAACCATAGGAGAGCATGCCCTTTTAATCGTTGATTCGTTAGCGAATCAACA
ATTCACTTTTTTAAGCAAAAAAGCGAATTGTGATCTTGCTTTAGACATGAAATTCTTTTTTTATCAATGTTTTCTTTTGG
GGGAATGGTGCAAAAATAATATTAATGTTTCAGGTTTTGCTTCTGTGGATATGACTGCTTTTAAAAAATATAAGTTCCCC
ATCCCACCCCTAGAGATCCAACAAGAGATCGTTAAGATTTTGGACGCTTTCACAGAATTAAACACAGAATTAAACACAGA
ATTAAACACAGAATTAAAAGCGCGCAAAAAGCAATATGAATATTACCAAAACATGCTTTTAGACTTTAACGATATTAACC
AAAGCCACAAAGACGCCAAAGAAAGATTGGCGCAAAAAACCTACCCTAAACGCTTGAAAACCTTACTCCAAACTCTAGCG
CCTAAGGGGGTGGAGTTTAGGAAGTTGGGGGAGGTGTGTGAAATTTTAGATAATCGGCGTATCCCAATTGCGAAAAATAA
AAGAAATCCGGGAATTTATCCTTATTATGGAGCAAATGGAATTCAAGATTATATTGATAGCTATATTTTTGATGGGGATT
TTGTGCTAGTTGGTGAAGATGGGAGCGTTATCAATAAGGATAATACTCCTGTTGTGAATTGGGCAAGCGGAAAAATATGG
GTGAACAATCATGCTCATGTGCTTCAAACAAAAAATGAACTAAAATTAAAGTTTTTATATTTTTATTTACAAACGATAGA
TGTTAGCTATTGTGTTGCTGGAACTCCGCCAAAAATCAACCAAGAAAATTTAAAAAAAATAGCAATCCCCATCCCACCCC
TAGAGATCCAACAAGAGATCGTTAAGATTTTGGATCAATTTTCAATTTTAACCACCGATTTATTAGCCGGTATCCCCGCT
GAAATAAAAGCGAGAAAAAAGCAATACGAATATTACCGAGAAAAATTATTGACCTTCAAGCCCCTAACCCCACTAAATAG
CAAAGAGCTTGCGTAA

Upstream 100 bases:

>100_bases
GCGATGTGGGGGTTACGCTTTTAGCCTTAGCCAATTCCATGCGCGCGATGAGAGCTTAAAGCCTTGATCGTATCATTAGC
CATTTAGAAAGGGGGGCAAA

Downstream 100 bases:

>100_bases
GCAAGCGATAGCCTCTTGAAAAAAAGCGGTCTTGGGGGGTTAGGGGGATCTTAAGGGGGTTAAGGGGGGTATTATCGCAA
AATACCCCCCTATCCCCTTA

Product: anti-codon nuclease masking agent (prrB)

Products: NA

Alternate protein names: S.EcoprrI; Type I restriction enzyme EcoprrI specificity protein; S protein [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MHKIERLLQTLAPKGVEFKTLEEVFEIKNGYTPSKNNPEFWKNGTIPWFRMEDIRENGRILKDSIQHITPKALKGKKLFP
KNSIIISTKATIGEHALLIVDSLANQQFTFLSKKANCDLALDMKFFFYQCFLLGEWCKNNINVSGFASVDMTAFKKYKFP
IPPLEIQQEIVKILDAFTELNTELNTELNTELKARKKQYEYYQNMLLDFNDINQSHKDAKERLAQKTYPKRLKTLLQTLA
PKGVEFRKLGEVCEILDNRRIPIAKNKRNPGIYPYYGANGIQDYIDSYIFDGDFVLVGEDGSVINKDNTPVVNWASGKIW
VNNHAHVLQTKNELKLKFLYFYLQTIDVSYCVAGTPPKINQENLKKIAIPIPPLEIQQEIVKILDQFSILTTDLLAGIPA
EIKARKKQYEYYREKLLTFKPLTPLNSKELA

Sequences:

>Translated_431_residues
MHKIERLLQTLAPKGVEFKTLEEVFEIKNGYTPSKNNPEFWKNGTIPWFRMEDIRENGRILKDSIQHITPKALKGKKLFP
KNSIIISTKATIGEHALLIVDSLANQQFTFLSKKANCDLALDMKFFFYQCFLLGEWCKNNINVSGFASVDMTAFKKYKFP
IPPLEIQQEIVKILDAFTELNTELNTELNTELKARKKQYEYYQNMLLDFNDINQSHKDAKERLAQKTYPKRLKTLLQTLA
PKGVEFRKLGEVCEILDNRRIPIAKNKRNPGIYPYYGANGIQDYIDSYIFDGDFVLVGEDGSVINKDNTPVVNWASGKIW
VNNHAHVLQTKNELKLKFLYFYLQTIDVSYCVAGTPPKINQENLKKIAIPIPPLEIQQEIVKILDQFSILTTDLLAGIPA
EIKARKKQYEYYREKLLTFKPLTPLNSKELA
>Mature_431_residues
MHKIERLLQTLAPKGVEFKTLEEVFEIKNGYTPSKNNPEFWKNGTIPWFRMEDIRENGRILKDSIQHITPKALKGKKLFP
KNSIIISTKATIGEHALLIVDSLANQQFTFLSKKANCDLALDMKFFFYQCFLLGEWCKNNINVSGFASVDMTAFKKYKFP
IPPLEIQQEIVKILDAFTELNTELNTELNTELKARKKQYEYYQNMLLDFNDINQSHKDAKERLAQKTYPKRLKTLLQTLA
PKGVEFRKLGEVCEILDNRRIPIAKNKRNPGIYPYYGANGIQDYIDSYIFDGDFVLVGEDGSVINKDNTPVVNWASGKIW
VNNHAHVLQTKNELKLKFLYFYLQTIDVSYCVAGTPPKINQENLKKIAIPIPPLEIQQEIVKILDQFSILTTDLLAGIPA
EIKARKKQYEYYREKLLTFKPLTPLNSKELA

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 49769; Mature: 49769

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKIERLLQTLAPKGVEFKTLEEVFEIKNGYTPSKNNPEFWKNGTIPWFRMEDIRENGRI
CCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEHHHHHHCCCC
LKDSIQHITPKALKGKKLFPKNSIIISTKATIGEHALLIVDSLANQQFTFLSKKANCDLA
HHHHHHHCCHHHHCCCCCCCCCCEEEEECCCCCCCCEEEEEHHCCCCEEEECCCCCCCEE
LDMKFFFYQCFLLGEWCKNNINVSGFASVDMTAFKKYKFPIPPLEIQQEIVKILDAFTEL
EHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
NTELNTELNTELKARKKQYEYYQNMLLDFNDINQSHKDAKERLAQKTYPKRLKTLLQTLA
CCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
PKGVEFRKLGEVCEILDNRRIPIAKNKRNPGIYPYYGANGIQDYIDSYIFDGDFVLVGED
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHEECCCEEEECCC
GSVINKDNTPVVNWASGKIWVNNHAHVLQTKNELKLKFLYFYLQTIDVSYCVAGTPPKIN
CCEECCCCCCEEEECCCEEEECCCEEEEECCCHHHHHHHHHHHHHHHHEEEECCCCCCCC
QENLKKIAIPIPPLEIQQEIVKILDQFSILTTDLLAGIPAEIKARKKQYEYYREKLLTFK
HHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCEEC
PLTPLNSKELA
CCCCCCCCCCC
>Mature Secondary Structure
MHKIERLLQTLAPKGVEFKTLEEVFEIKNGYTPSKNNPEFWKNGTIPWFRMEDIRENGRI
CCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEHHHHHHCCCC
LKDSIQHITPKALKGKKLFPKNSIIISTKATIGEHALLIVDSLANQQFTFLSKKANCDLA
HHHHHHHCCHHHHCCCCCCCCCCEEEEECCCCCCCCEEEEEHHCCCCEEEECCCCCCCEE
LDMKFFFYQCFLLGEWCKNNINVSGFASVDMTAFKKYKFPIPPLEIQQEIVKILDAFTEL
EHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
NTELNTELNTELKARKKQYEYYQNMLLDFNDINQSHKDAKERLAQKTYPKRLKTLLQTLA
CCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
PKGVEFRKLGEVCEILDNRRIPIAKNKRNPGIYPYYGANGIQDYIDSYIFDGDFVLVGED
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHEECCCEEEECCC
GSVINKDNTPVVNWASGKIWVNNHAHVLQTKNELKLKFLYFYLQTIDVSYCVAGTPPKIN
CCEECCCCCCEEEECCCEEEECCCEEEEECCCHHHHHHHHHHHHHHHHEEEECCCCCCCC
QENLKKIAIPIPPLEIQQEIVKILDQFSILTTDLLAGIPAEIKARKKQYEYYREKLLTFK
HHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCEEC
PLTPLNSKELA
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1691706 [H]