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Identifier: 15645091

GI number: 15645091

Start: 481319

End: 482782

Strand: Reverse

Name: Not Available

Synonym: HP0463

Alternate gene names: 15645091

Gene position: 482782-481319 (Counterclockwise)

Preceding gene: 15645092

Following gene: 15645090

Centisome position: 28.95

GC content: 37.23

Gene sequence:

>1464_bases
ATGCCTAATAACGCTTTATTGCAAATCAAACAAGACACCCTAAGCCTCATTGACGATTTAAAAGTCATTTGCACGAGTTT
TGGTTTAGGGAACGATGGCAACGAATACAAAATCATCACGCAATGCTTTTTGTATAAATTCTTATGCGATAAGTTTGAAT
TCCTTTTTGAACAAGAATTCCCCAACCAAACGATACAAGATTACAAAGACTTTAACAAGGAAGAAAAAGAAGAGTTCTTT
ATTACCTTAACCGATAAAAGACTCCCCAAACTCGCCTATGATGATCTTTTAAACTATCTTTTTGAAAAACATTTTAACGA
TAACGATTTACACCTAAAGCTAGATATTATTTTTAATCGTATTTCTAGCAATAATGCCGAGCTTTTCAATACCACAAGCA
CGGACAAAACCACTATCGCTTTATTTGAAAGCATCTCACAATACATTAATGAAGAGTCTAAAAGGGCTAATTTTACAAGA
GTTTTACTGGACAAACTCAAAAAGTTTAATTTCAAACAAGCTTTTTTAAACTTACAAAACCAACAAGGCTATGATTTTTT
CGCCCCCATTTTTGAATACTTACTCAAAGATTACAATAACGCCGGCGGAGGCAAATACGCCGAATACTACACCCCTTTAA
GCATCGCTAGCATCATTGCTAAGCTTTTGATTAATGAGCCGACTAGAAATGTCAAAATCTATGACCCAAGTGCAGGCACA
GGAACGCTTTTAATGGCATTAGCCCACCAAATAGGCACGGATTCTTGCACCCTTTATGCCCAAGACATTTCGCAAAAATC
CTTAAGAATGCTCAAACTCAACTTGATTTTAAACGACTTGACCCACTCTTTAAGAAACGCCATTGAGGGAAACACTTTAA
CCAACCCCTACCATTCTAAAGACTTTAAGGGGAAAATGGATTACATCGTGAGTAACCCCCCTTTCAAGCTGGATTTTTCC
AACGAGCATGCCGAAATTTCGCAAAACAAAAACGATTTTTTCTTAGGCGTGCCTAATATCCCTAAAAACAATAAAAGCAA
AATGCCCATTTACACGCTCTTTTTCCAGCATTGCTTGAACATGCTTAGTAATAAGGGTAAGGGGGCTATTATCGTGCCAA
CCGGATTCATCAGCGCTAAAAGCGGGGTAGAAAATAAGATTATCCGGCATTTAGTGGATGAAAGGCTCGTTTATGGGGTG
GTTTGCATGCCCAGTCAGGTTTTTGCCAACACCGGCACTAACGTGAGCATCATCTTTTTTCAAAAAACGCCAAGCGCAAA
GGAAGTGGTTTTGATTGACGCTTCCAAACTCGGCGAAGAATACACCGAAAACAAAAACAAAAAAACGCGCTTAAGACCAA
GCGATATTGATTTGATTTTAGAAACTTTTCAAAATAAAACTAAAAAATCGGATTTTTGCGCTCTGGTTTCTTTTGATGAA
ATTACAGAAAAAATTATTCTCTAA

Upstream 100 bases:

>100_bases
AAAAAAGACCCCTCCTTAAAAGATTTAGAAAAAGAAAAAGAACTTATCATTCAAACCCTTTTTAACGAACTCACACAAAA
CCACCATCAAGGAAATCCGC

Downstream 100 bases:

>100_bases
ACCCTGGGCAGTATTTCATTATAGAAGACACGAGCGAGAAAATCAGCCAAGCGGAGTTTGAAAATTTAATGCAACAATAT
TCAAGCGAACTGACAAGCCT

Product: type I restriction enzyme M protein (hsdM)

Products: NA

Alternate protein names: M.MpnORFDP [H]

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MPNNALLQIKQDTLSLIDDLKVICTSFGLGNDGNEYKIITQCFLYKFLCDKFEFLFEQEFPNQTIQDYKDFNKEEKEEFF
ITLTDKRLPKLAYDDLLNYLFEKHFNDNDLHLKLDIIFNRISSNNAELFNTTSTDKTTIALFESISQYINEESKRANFTR
VLLDKLKKFNFKQAFLNLQNQQGYDFFAPIFEYLLKDYNNAGGGKYAEYYTPLSIASIIAKLLINEPTRNVKIYDPSAGT
GTLLMALAHQIGTDSCTLYAQDISQKSLRMLKLNLILNDLTHSLRNAIEGNTLTNPYHSKDFKGKMDYIVSNPPFKLDFS
NEHAEISQNKNDFFLGVPNIPKNNKSKMPIYTLFFQHCLNMLSNKGKGAIIVPTGFISAKSGVENKIIRHLVDERLVYGV
VCMPSQVFANTGTNVSIIFFQKTPSAKEVVLIDASKLGEEYTENKNKKTRLRPSDIDLILETFQNKTKKSDFCALVSFDE
ITEKIIL

Sequences:

>Translated_487_residues
MPNNALLQIKQDTLSLIDDLKVICTSFGLGNDGNEYKIITQCFLYKFLCDKFEFLFEQEFPNQTIQDYKDFNKEEKEEFF
ITLTDKRLPKLAYDDLLNYLFEKHFNDNDLHLKLDIIFNRISSNNAELFNTTSTDKTTIALFESISQYINEESKRANFTR
VLLDKLKKFNFKQAFLNLQNQQGYDFFAPIFEYLLKDYNNAGGGKYAEYYTPLSIASIIAKLLINEPTRNVKIYDPSAGT
GTLLMALAHQIGTDSCTLYAQDISQKSLRMLKLNLILNDLTHSLRNAIEGNTLTNPYHSKDFKGKMDYIVSNPPFKLDFS
NEHAEISQNKNDFFLGVPNIPKNNKSKMPIYTLFFQHCLNMLSNKGKGAIIVPTGFISAKSGVENKIIRHLVDERLVYGV
VCMPSQVFANTGTNVSIIFFQKTPSAKEVVLIDASKLGEEYTENKNKKTRLRPSDIDLILETFQNKTKKSDFCALVSFDE
ITEKIIL
>Mature_486_residues
PNNALLQIKQDTLSLIDDLKVICTSFGLGNDGNEYKIITQCFLYKFLCDKFEFLFEQEFPNQTIQDYKDFNKEEKEEFFI
TLTDKRLPKLAYDDLLNYLFEKHFNDNDLHLKLDIIFNRISSNNAELFNTTSTDKTTIALFESISQYINEESKRANFTRV
LLDKLKKFNFKQAFLNLQNQQGYDFFAPIFEYLLKDYNNAGGGKYAEYYTPLSIASIIAKLLINEPTRNVKIYDPSAGTG
TLLMALAHQIGTDSCTLYAQDISQKSLRMLKLNLILNDLTHSLRNAIEGNTLTNPYHSKDFKGKMDYIVSNPPFKLDFSN
EHAEISQNKNDFFLGVPNIPKNNKSKMPIYTLFFQHCLNMLSNKGKGAIIVPTGFISAKSGVENKIIRHLVDERLVYGVV
CMPSQVFANTGTNVSIIFFQKTPSAKEVVLIDASKLGEEYTENKNKKTRLRPSDIDLILETFQNKTKKSDFCALVSFDEI
TEKIIL

Specific function: Methylation of specific adenine residues; required for both restriction and modification activities [H]

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296
- InterPro:   IPR004546 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 55801; Mature: 55670

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNNALLQIKQDTLSLIDDLKVICTSFGLGNDGNEYKIITQCFLYKFLCDKFEFLFEQEF
CCCCCEEEECHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
PNQTIQDYKDFNKEEKEEFFITLTDKRLPKLAYDDLLNYLFEKHFNDNDLHLKLDIIFNR
CCCHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEH
ISSNNAELFNTTSTDKTTIALFESISQYINEESKRANFTRVLLDKLKKFNFKQAFLNLQN
HCCCCCEEEECCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCC
QQGYDFFAPIFEYLLKDYNNAGGGKYAEYYTPLSIASIIAKLLINEPTRNVKIYDPSAGT
CCCCHHHHHHHHHHHHHCCCCCCCCCHHHCCHHHHHHHHHHHHHCCCCCCEEEECCCCCH
GTLLMALAHQIGTDSCTLYAQDISQKSLRMLKLNLILNDLTHSLRNAIEGNTLTNPYHSK
HHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
DFKGKMDYIVSNPPFKLDFSNEHAEISQNKNDFFLGVPNIPKNNKSKMPIYTLFFQHCLN
CCCCCCCEEECCCCEEEECCCCCHHHCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHH
MLSNKGKGAIIVPTGFISAKSGVENKIIRHLVDERLVYGVVCMPSQVFANTGTNVSIIFF
HHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHEEEEECCHHHHHCCCCCEEEEEE
QKTPSAKEVVLIDASKLGEEYTENKNKKTRLRPSDIDLILETFQNKTKKSDFCALVSFDE
ECCCCCCEEEEEEHHHHCHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEHHH
ITEKIIL
HHHHHCC
>Mature Secondary Structure 
PNNALLQIKQDTLSLIDDLKVICTSFGLGNDGNEYKIITQCFLYKFLCDKFEFLFEQEF
CCCCEEEECHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
PNQTIQDYKDFNKEEKEEFFITLTDKRLPKLAYDDLLNYLFEKHFNDNDLHLKLDIIFNR
CCCHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEH
ISSNNAELFNTTSTDKTTIALFESISQYINEESKRANFTRVLLDKLKKFNFKQAFLNLQN
HCCCCCEEEECCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCC
QQGYDFFAPIFEYLLKDYNNAGGGKYAEYYTPLSIASIIAKLLINEPTRNVKIYDPSAGT
CCCCHHHHHHHHHHHHHCCCCCCCCCHHHCCHHHHHHHHHHHHHCCCCCCEEEECCCCCH
GTLLMALAHQIGTDSCTLYAQDISQKSLRMLKLNLILNDLTHSLRNAIEGNTLTNPYHSK
HHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
DFKGKMDYIVSNPPFKLDFSNEHAEISQNKNDFFLGVPNIPKNNKSKMPIYTLFFQHCLN
CCCCCCCEEECCCCEEEECCCCCHHHCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHH
MLSNKGKGAIIVPTGFISAKSGVENKIIRHLVDERLVYGVVCMPSQVFANTGTNVSIIFF
HHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHEEEEECCHHHHHCCCCCEEEEEE
QKTPSAKEVVLIDASKLGEEYTENKNKKTRLRPSDIDLILETFQNKTKKSDFCALVSFDE
ECCCCCCEEEEEEHHHHCHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEHHH
ITEKIIL
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]