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Identifier: 15645068

GI number: 15645068

Start: 457297

End: 459330

Strand: Reverse

Name: Not Available

Synonym: HP0440

Alternate gene names: 15645068

Gene position: 459330-457297 (Counterclockwise)

Preceding gene: 15645069

Following gene: 15645067

Centisome position: 27.54

GC content: 33.2

Gene sequence:

>2034_bases
ATGAAAGATAGCGTGATTATCATTGAAAGCCCTAATAAGGTTGAAAAGATTAAACAACTAACAGGTGCGGAAGTTTATGC
AACTATAGGGCATTTTACTAACCTTATAGGCGTAGATATTGAAAATAATTTTGCTTGTAAGTTTGAAATCAAAGAAGACA
AGGCTAAGAAAATAAATTTTTTAATCAATGCTTGTAGGGACAAAAAAGTCTATATCGCTACTGACCCTGATAGAGAGGGC
TATGGTATTGGCTATCAATTTTATGAAAAAATTAAAAATGTAGCTAGTGAAATTTATCGTGCAGAATTTCATGAGATCAC
ACCAAGCGGTATTGAAAGCGGCTTAAAAAATGCGGTGTTATTTTCTCGTTCTAACACTAATTTATACCAATCATTCTTAG
GTAGGATTGCAAGCGATCAGGTTGTTGGATTTGCTCTAACAAGCTATTTAAGAAAAAGTTTAAATCTTAGTGAATTGAGC
GCTGGTAGAGTGCAAACTCCAGCTCTAGCTTTAATCTGCCAAAGAGATCAAGAAATAAGGGATTTTGATAAGTTGGATGC
TGAAGAAAAAGTAGAATATCAAATCCAAGCTAATATTGTGTGCAATGAAAAAGAAGCGATTATTAAGCATGTAAGAGCGA
ATGAAAAAAACGAACTAGTGGATTTTAAATTTAAAGACAAAAACGARGCATCTCAATTTTTAAAAGATCTAAAGGACGGA
TTAGGGTCTATGAGTGTTCTAGTTTCATTAAAAGAAAGTCTATCCAATAAAGAACCCAAAAAACCTTTCACCACCTCTAA
ACTTTTATCCCAAGCGAGCAAATCCTTAAAAATACCCACTAAAGAAATCGCACAACTTGCTCAAAAACTCTTTGAAGCTG
GTCTCATCACTTATCACAGAACTGATAGTGAGTTTTTAAGTCCTGAGTATCTCAAAGAACATGAAGTGTTTTTTGAACCT
ATTTATCCGAGTGTTTATCAATACAGGGAATATAAAGCTGGCAAAAACTCACAAGCTGAAGCGCATGAGGCTATAAGAAT
TACACATCCGCATGCTTTAAAAGACTTAGAAAAAGTGTGCAGTGACGCTAAAATAAGCGAAGAATTAGCGCTGAAGTTGT
ATCAACTTATTTATACCAATACAATTTGTTCTCAAAGTAGAAACGCCTTATACAATCAATACGATTGTATCTTTAAAATC
AAAAGCGAGAGTTTTAAGCTCTCTTTCAAACTTTTAAAAGAAAAGGGTTTTTTAGAGATTGAAGAACTCATTCAAGGCAA
AGAAGAAATTAACAGGGAAGAGCAAGAAAGTGAAATAGAAAACTTTTCATTAAAAGAAAATGATAGTGTCCCGTTAAAAG
AAGTTTTTATTAAAAAAATAGAAAAACCCAGTCCCAAACCTTATAAAGAAAGTGCCTTTATTCCCTTATTAGAAAGCGAG
GGGATTGGTCGTCCTAGCACTTATGCGAGCTTCTTAGATCTTCTTTTGAAGCGTAAATATATTAGCATTGATACAAAAAC
TAACGCCATCACCCCTACTAGTCAAGGATTAGAAGTGATTTCATTTTTCAAAAAAGATAAAGAAGTGGATTTCATCGCTC
TTACAAGCAAGGATAAGAGCAAATTAGGAAACACTACCAAACAATTTGAAGAATGTTTGGATTTAATTATGAGAGGTGAA
GCTAGTTATGAAAAGTTCATGCTTGAAGTCATAAGCAAATTAAAAAGTACCGCCAAGTTTTATCAAACACAATCTACTGA
CAACAACATGCCTACTGATAAGCAACTTGAACTCATAGACAAAATCTGTAAAGATAAAAAATTACAAAAACCCAGTCAAG
AAATCCTGCAGAACAAGGAAAAATGTTCTGATTGGATTGCAGAGCATGTTAAAGAAAAACCTAGTCAAAAACAACTAAAT
CTGGTTAAAAAAATTTGCGAAGAGTGTAAATTGGCAATGCCCACCAATAAAATATTGAATGACAAATTCGCTATCTCTAA
ATGGATTGATAAACACAAAAAGACAAAGAAATGA

Upstream 100 bases:

>100_bases
GAGCGTATAACTCTAGCGCGTCTAGCGTTTTTGAACTGGAAACGCTTAAAAAGGATAATCCAAAGGATTATAAAGAAATT
TACTTGACAAAGGAGTAGGA

Downstream 100 bases:

>100_bases
GAATATTTTTAATAGGAATAATTTTGTTAGCACTAAGTGGATGTTCTAACAAACAATATGAAATGTATAAAAGCCCTTGT
GCCTTTTTTCACTTAAATCA

Product: DNA topoisomerase I (topA)

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 677; Mature: 677

Protein sequence:

>677_residues
MKDSVIIIESPNKVEKIKQLTGAEVYATIGHFTNLIGVDIENNFACKFEIKEDKAKKINFLINACRDKKVYIATDPDREG
YGIGYQFYEKIKNVASEIYRAEFHEITPSGIESGLKNAVLFSRSNTNLYQSFLGRIASDQVVGFALTSYLRKSLNLSELS
AGRVQTPALALICQRDQEIRDFDKLDAEEKVEYQIQANIVCNEKEAIIKHVRANEKNELVDFKFKDKNEASQFLKDLKDG
LGSMSVLVSLKESLSNKEPKKPFTTSKLLSQASKSLKIPTKEIAQLAQKLFEAGLITYHRTDSEFLSPEYLKEHEVFFEP
IYPSVYQYREYKAGKNSQAEAHEAIRITHPHALKDLEKVCSDAKISEELALKLYQLIYTNTICSQSRNALYNQYDCIFKI
KSESFKLSFKLLKEKGFLEIEELIQGKEEINREEQESEIENFSLKENDSVPLKEVFIKKIEKPSPKPYKESAFIPLLESE
GIGRPSTYASFLDLLLKRKYISIDTKTNAITPTSQGLEVISFFKKDKEVDFIALTSKDKSKLGNTTKQFEECLDLIMRGE
ASYEKFMLEVISKLKSTAKFYQTQSTDNNMPTDKQLELIDKICKDKKLQKPSQEILQNKEKCSDWIAEHVKEKPSQKQLN
LVKKICEECKLAMPTNKILNDKFAISKWIDKHKKTKK

Sequences:

>Translated_677_residues
MKDSVIIIESPNKVEKIKQLTGAEVYATIGHFTNLIGVDIENNFACKFEIKEDKAKKINFLINACRDKKVYIATDPDREG
YGIGYQFYEKIKNVASEIYRAEFHEITPSGIESGLKNAVLFSRSNTNLYQSFLGRIASDQVVGFALTSYLRKSLNLSELS
AGRVQTPALALICQRDQEIRDFDKLDAEEKVEYQIQANIVCNEKEAIIKHVRANEKNELVDFKFKDKNEASQFLKDLKDG
LGSMSVLVSLKESLSNKEPKKPFTTSKLLSQASKSLKIPTKEIAQLAQKLFEAGLITYHRTDSEFLSPEYLKEHEVFFEP
IYPSVYQYREYKAGKNSQAEAHEAIRITHPHALKDLEKVCSDAKISEELALKLYQLIYTNTICSQSRNALYNQYDCIFKI
KSESFKLSFKLLKEKGFLEIEELIQGKEEINREEQESEIENFSLKENDSVPLKEVFIKKIEKPSPKPYKESAFIPLLESE
GIGRPSTYASFLDLLLKRKYISIDTKTNAITPTSQGLEVISFFKKDKEVDFIALTSKDKSKLGNTTKQFEECLDLIMRGE
ASYEKFMLEVISKLKSTAKFYQTQSTDNNMPTDKQLELIDKICKDKKLQKPSQEILQNKEKCSDWIAEHVKEKPSQKQLN
LVKKICEECKLAMPTNKILNDKFAISKWIDKHKKTKK
>Mature_677_residues
MKDSVIIIESPNKVEKIKQLTGAEVYATIGHFTNLIGVDIENNFACKFEIKEDKAKKINFLINACRDKKVYIATDPDREG
YGIGYQFYEKIKNVASEIYRAEFHEITPSGIESGLKNAVLFSRSNTNLYQSFLGRIASDQVVGFALTSYLRKSLNLSELS
AGRVQTPALALICQRDQEIRDFDKLDAEEKVEYQIQANIVCNEKEAIIKHVRANEKNELVDFKFKDKNEASQFLKDLKDG
LGSMSVLVSLKESLSNKEPKKPFTTSKLLSQASKSLKIPTKEIAQLAQKLFEAGLITYHRTDSEFLSPEYLKEHEVFFEP
IYPSVYQYREYKAGKNSQAEAHEAIRITHPHALKDLEKVCSDAKISEELALKLYQLIYTNTICSQSRNALYNQYDCIFKI
KSESFKLSFKLLKEKGFLEIEELIQGKEEINREEQESEIENFSLKENDSVPLKEVFIKKIEKPSPKPYKESAFIPLLESE
GIGRPSTYASFLDLLLKRKYISIDTKTNAITPTSQGLEVISFFKKDKEVDFIALTSKDKSKLGNTTKQFEECLDLIMRGE
ASYEKFMLEVISKLKSTAKFYQTQSTDNNMPTDKQLELIDKICKDKKLQKPSQEILQNKEKCSDWIAEHVKEKPSQKQLN
LVKKICEECKLAMPTNKILNDKFAISKWIDKHKKTKK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=534, Percent_Identity=21.9101123595506, Blast_Score=87, Evalue=5e-17,
Organism=Escherichia coli, GI1787529, Length=545, Percent_Identity=26.7889908256881, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1788061, Length=554, Percent_Identity=22.2021660649819, Blast_Score=81, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 77678; Mature: 77678

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDSVIIIESPNKVEKIKQLTGAEVYATIGHFTNLIGVDIENNFACKFEIKEDKAKKINF
CCCCEEEEECCCHHHHHHHHCCCHHHHHHHHHHHHEEEEECCCEEEEEEECCCHHHHHHH
LINACRDKKVYIATDPDREGYGIGYQFYEKIKNVASEIYRAEFHEITPSGIESGLKNAVL
HHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEE
FSRSNTNLYQSFLGRIASDQVVGFALTSYLRKSLNLSELSAGRVQTPALALICQRDQEIR
EECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHCCHHHH
DFDKLDAEEKVEYQIQANIVCNEKEAIIKHVRANEKNELVDFKFKDKNEASQFLKDLKDG
HHHHCCCHHHEEEEEEEEEEECCHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHH
LGSMSVLVSLKESLSNKEPKKPFTTSKLLSQASKSLKIPTKEIAQLAQKLFEAGLITYHR
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEC
TDSEFLSPEYLKEHEVFFEPIYPSVYQYREYKAGKNSQAEAHEAIRITHPHALKDLEKVC
CCHHHCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHEEECCCHHHHHHHHHH
SDAKISEELALKLYQLIYTNTICSQSRNALYNQYDCIFKIKSESFKLSFKLLKEKGFLEI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCCCHH
EELIQGKEEINREEQESEIENFSLKENDSVPLKEVFIKKIEKPSPKPYKESAFIPLLESE
HHHHCCHHHHCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEECCCC
GIGRPSTYASFLDLLLKRKYISIDTKTNAITPTSQGLEVISFFKKDKEVDFIALTSKDKS
CCCCCHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCHH
KLGNTTKQFEECLDLIMRGEASYEKFMLEVISKLKSTAKFYQTQSTDNNMPTDKQLELID
HHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
KICKDKKLQKPSQEILQNKEKCSDWIAEHVKEKPSQKQLNLVKKICEECKLAMPTNKILN
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHH
DKFAISKWIDKHKKTKK
HHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKDSVIIIESPNKVEKIKQLTGAEVYATIGHFTNLIGVDIENNFACKFEIKEDKAKKINF
CCCCEEEEECCCHHHHHHHHCCCHHHHHHHHHHHHEEEEECCCEEEEEEECCCHHHHHHH
LINACRDKKVYIATDPDREGYGIGYQFYEKIKNVASEIYRAEFHEITPSGIESGLKNAVL
HHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEE
FSRSNTNLYQSFLGRIASDQVVGFALTSYLRKSLNLSELSAGRVQTPALALICQRDQEIR
EECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHCCHHHH
DFDKLDAEEKVEYQIQANIVCNEKEAIIKHVRANEKNELVDFKFKDKNEASQFLKDLKDG
HHHHCCCHHHEEEEEEEEEEECCHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHH
LGSMSVLVSLKESLSNKEPKKPFTTSKLLSQASKSLKIPTKEIAQLAQKLFEAGLITYHR
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEC
TDSEFLSPEYLKEHEVFFEPIYPSVYQYREYKAGKNSQAEAHEAIRITHPHALKDLEKVC
CCHHHCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHEEECCCHHHHHHHHHH
SDAKISEELALKLYQLIYTNTICSQSRNALYNQYDCIFKIKSESFKLSFKLLKEKGFLEI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCCCHH
EELIQGKEEINREEQESEIENFSLKENDSVPLKEVFIKKIEKPSPKPYKESAFIPLLESE
HHHHCCHHHHCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEECCCC
GIGRPSTYASFLDLLLKRKYISIDTKTNAITPTSQGLEVISFFKKDKEVDFIALTSKDKS
CCCCCHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCHH
KLGNTTKQFEECLDLIMRGEASYEKFMLEVISKLKSTAKFYQTQSTDNNMPTDKQLELID
HHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
KICKDKKLQKPSQEILQNKEKCSDWIAEHVKEKPSQKQLNLVKKICEECKLAMPTNKILN
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHH
DKFAISKWIDKHKKTKK
HHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]