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The map label for this gene is pheT

Identifier: 15645030

GI number: 15645030

Start: 413341

End: 415635

Strand: Reverse

Name: pheT

Synonym: HP0402

Alternate gene names: 15645030

Gene position: 415635-413341 (Counterclockwise)

Preceding gene: 15645031

Following gene: 15645029

Centisome position: 24.92

GC content: 39.13

Gene sequence:

>2295_bases
ATGAAACTGAGCATTAATGATTTGAATGTTTTTGTCAATACGCCTAAAGATATAGCCAAACTCTGTGAGGATTTGAGTCG
CTTAGGTTTAGAAGTGGAAAGCTGTATCCCTTGTATCGCTCCTAAAAATGTGGTTGTGGGTAAAATTTTAGAAAAAGCCC
CCCATAAAAACGCTGAAAAACTCAGCGTGTGTCAAGTGGATGTGGGTAAAGAAGTGTTGCAAATCGTGTGTGGGGCTAAA
AATGTCGCGCCAAACCAATTCGTGCCAGTCGCTTTAAACGGGGCGCTAATCGGCTCAACCACCATCGCTAAAACGGAGCT
TAGGGGGGTTGAAAGCCATGGCATGATTTGCTCTAGCATTGAATTAGGCTTCCCTAAAATCAATGATGGCATCTTGGAAT
TAGATGAGAGCGTTGGGGAGTTGGTTTTAGGGAAAGAATTAAACGAATACGCCCCTTTCAACACGCATGTTTTAGAAATT
TCATTGACTCCCAATCGTGGGGATTGCTTGAGCGTTTTAGGTATTGCCAGAGAAATTAGCGCCTTTTATCACACGCCCCT
AAAGCCTATTAAGGCTTTAAATTTTACGCCCAAAAGCGGTTTGATCACGCTTAGTGCGGGTGAAAATATTGAATCGCATC
TGGCTTATTATTTGATTTGCAACCATTCATTAAAAACCCCTTTAAATATCAAACTTTCGCTCGCTCATAATAATGCCTTG
AGTGAGAACGATCTGAACAATTTCATAGAATTTAGCACGCATTTTAGTGGGGTAATAATGAACGCTTATAGCCTAAATAC
AACCCCTATGGATTTGAGCGTGAAAAACGATGAAAACAACCTTGAAAGCGTTTATATCAACCATCAAAAACGCTCCACGA
TCGCTATCAAGCATCAAGTTCAAAAAGATTTGAGCGAGTGTTTGCTTTTAGAGGCAAGTTACACCGATCCGATAAGCCTG
TCTTTAAAATTACACGCCCTAAAAGATAAAACGCTTCAAAAAGACAACGCCCTTATTTATAGAAGCGCTAGGGGGAGTAA
CCCTAATTTATCAGACGGCTTGAATTTTTTAAGCGCTCATTTGAAAGCCACGATTTTAGAAAGCAAACAAACTGAGCATT
CTTTAAAAGATCGCACCCTTACATTCCAGCTTGAAGACATTACTGAAATTTTGGGGCTTGCTGTAGAGAAAGAAAAAATT
CAAGGCATTTTAAAAAATTTAGGCTTTAAAGTCAGCGTAAAAGAGCCAAACTCAAAACCCCAAATTTTAGAGGTTATTGC
GCCAAATTTCAGGCATGACATTAAAACGATCCAAGATATTGCTGAAGAAATTTTGCGCTTTGTAGGGATTGATAATCTAG
TCTCAAAGCCCCTTCATTGTGTCAGTAGCAAAAATTCAAACCCCAATTACGACACGCACCGCTTTTTTGAAAACCTTAAA
CACAAGGCTCTCGCTTGCGGTTTTAAAGAAGTCATTCATTACGTGTTTTACTCTAAAGAAAAACAGCAAAAATTAGGCTT
TGAAGTTTTAGAAGATCCCCTAGAATTGCAAAACCCTATCACAACGGAGTTAAACACCCTAAGGACGAGTCTTGTTTGCG
GGCTTTTAGACGCCAGTTTAAGGAATAAAAATTTAGGGTTTAAAAGCATAGCCCTTTATGAAAAGGGGAGCGTGTATAAC
TCTAAAAGAGAAGAAATCCAAAAACTAGGCTTTTTAATAAGCGGCTTGCAAAAAAAAGAAAGCTACCCTGATACTAAGGG
CAAGGCTTGGGATTTTTACTCTTTTGCCGAATGCGTTTCAAAAGTTATAGGGGATTTCAGCTTGGAAAAACTAACCACTC
AAACCCCCATTAACCACCCCTACCAGAGCGCTAAAATCATTCAAAATCATGAAATCATAGGCGTGATCGCTAAAATCCAC
CCTAAAGTGATCCAGGAATTGGATTTGTTTGAAAGCTATTACGCTGAGATAGACGCTTTTAAACTCAAACGCCCTGCTAT
GCTATTAAAACCCTTTAGCATTTATCCTAGCAGTGTTAGGGATTTGACTCTCATCATTGATGAGAATACCGCTTTTAGTG
GGATTAAAAAAGCCCTAAAGGACGCTCAAATCCCTAATTTAAGCGAGATTCTACCCCTTGATATTTTTAAAGAAAGTAAT
AATTCCATAGCCTTAAGCGTGCGTTGCGTGATCCATTCTTTAGAAAAAACCCTGAATGATGAAGAGGTCAATTCAGCCGT
GCAAAAAGCACTTGAAATTTTAGAAAAAGAATTTAACGCCCGCCTTAAAGGATAA

Upstream 100 bases:

>100_bases
CTTTTGGCATGGGGATTGAAAGATTAGCCATGCTGACTTGCCAGATCAATGATTTGCGCAGTTTCTTTGAAACTGATTTG
AGAGTGTTGGAGAGCTTTTA

Downstream 100 bases:

>100_bases
TATAAAGGATAATATGTGATAGAGCTTGACATTAACGCTAGCGATAAATCGCTCTCACACAGAGCCGTTATTTTTAGCCT
GCTCGCTCAAAAACCTTGTT

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]

Number of amino acids: Translated: 764; Mature: 764

Protein sequence:

>764_residues
MKLSINDLNVFVNTPKDIAKLCEDLSRLGLEVESCIPCIAPKNVVVGKILEKAPHKNAEKLSVCQVDVGKEVLQIVCGAK
NVAPNQFVPVALNGALIGSTTIAKTELRGVESHGMICSSIELGFPKINDGILELDESVGELVLGKELNEYAPFNTHVLEI
SLTPNRGDCLSVLGIAREISAFYHTPLKPIKALNFTPKSGLITLSAGENIESHLAYYLICNHSLKTPLNIKLSLAHNNAL
SENDLNNFIEFSTHFSGVIMNAYSLNTTPMDLSVKNDENNLESVYINHQKRSTIAIKHQVQKDLSECLLLEASYTDPISL
SLKLHALKDKTLQKDNALIYRSARGSNPNLSDGLNFLSAHLKATILESKQTEHSLKDRTLTFQLEDITEILGLAVEKEKI
QGILKNLGFKVSVKEPNSKPQILEVIAPNFRHDIKTIQDIAEEILRFVGIDNLVSKPLHCVSSKNSNPNYDTHRFFENLK
HKALACGFKEVIHYVFYSKEKQQKLGFEVLEDPLELQNPITTELNTLRTSLVCGLLDASLRNKNLGFKSIALYEKGSVYN
SKREEIQKLGFLISGLQKKESYPDTKGKAWDFYSFAECVSKVIGDFSLEKLTTQTPINHPYQSAKIIQNHEIIGVIAKIH
PKVIQELDLFESYYAEIDAFKLKRPAMLLKPFSIYPSSVRDLTLIIDENTAFSGIKKALKDAQIPNLSEILPLDIFKESN
NSIALSVRCVIHSLEKTLNDEEVNSAVQKALEILEKEFNARLKG

Sequences:

>Translated_764_residues
MKLSINDLNVFVNTPKDIAKLCEDLSRLGLEVESCIPCIAPKNVVVGKILEKAPHKNAEKLSVCQVDVGKEVLQIVCGAK
NVAPNQFVPVALNGALIGSTTIAKTELRGVESHGMICSSIELGFPKINDGILELDESVGELVLGKELNEYAPFNTHVLEI
SLTPNRGDCLSVLGIAREISAFYHTPLKPIKALNFTPKSGLITLSAGENIESHLAYYLICNHSLKTPLNIKLSLAHNNAL
SENDLNNFIEFSTHFSGVIMNAYSLNTTPMDLSVKNDENNLESVYINHQKRSTIAIKHQVQKDLSECLLLEASYTDPISL
SLKLHALKDKTLQKDNALIYRSARGSNPNLSDGLNFLSAHLKATILESKQTEHSLKDRTLTFQLEDITEILGLAVEKEKI
QGILKNLGFKVSVKEPNSKPQILEVIAPNFRHDIKTIQDIAEEILRFVGIDNLVSKPLHCVSSKNSNPNYDTHRFFENLK
HKALACGFKEVIHYVFYSKEKQQKLGFEVLEDPLELQNPITTELNTLRTSLVCGLLDASLRNKNLGFKSIALYEKGSVYN
SKREEIQKLGFLISGLQKKESYPDTKGKAWDFYSFAECVSKVIGDFSLEKLTTQTPINHPYQSAKIIQNHEIIGVIAKIH
PKVIQELDLFESYYAEIDAFKLKRPAMLLKPFSIYPSSVRDLTLIIDENTAFSGIKKALKDAQIPNLSEILPLDIFKESN
NSIALSVRCVIHSLEKTLNDEEVNSAVQKALEILEKEFNARLKG
>Mature_764_residues
MKLSINDLNVFVNTPKDIAKLCEDLSRLGLEVESCIPCIAPKNVVVGKILEKAPHKNAEKLSVCQVDVGKEVLQIVCGAK
NVAPNQFVPVALNGALIGSTTIAKTELRGVESHGMICSSIELGFPKINDGILELDESVGELVLGKELNEYAPFNTHVLEI
SLTPNRGDCLSVLGIAREISAFYHTPLKPIKALNFTPKSGLITLSAGENIESHLAYYLICNHSLKTPLNIKLSLAHNNAL
SENDLNNFIEFSTHFSGVIMNAYSLNTTPMDLSVKNDENNLESVYINHQKRSTIAIKHQVQKDLSECLLLEASYTDPISL
SLKLHALKDKTLQKDNALIYRSARGSNPNLSDGLNFLSAHLKATILESKQTEHSLKDRTLTFQLEDITEILGLAVEKEKI
QGILKNLGFKVSVKEPNSKPQILEVIAPNFRHDIKTIQDIAEEILRFVGIDNLVSKPLHCVSSKNSNPNYDTHRFFENLK
HKALACGFKEVIHYVFYSKEKQQKLGFEVLEDPLELQNPITTELNTLRTSLVCGLLDASLRNKNLGFKSIALYEKGSVYN
SKREEIQKLGFLISGLQKKESYPDTKGKAWDFYSFAECVSKVIGDFSLEKLTTQTPINHPYQSAKIIQNHEIIGVIAKIH
PKVIQELDLFESYYAEIDAFKLKRPAMLLKPFSIYPSSVRDLTLIIDENTAFSGIKKALKDAQIPNLSEILPLDIFKESN
NSIALSVRCVIHSLEKTLNDEEVNSAVQKALEILEKEFNARLKG

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788006, Length=819, Percent_Identity=26.984126984127, Blast_Score=212, Evalue=8e-56,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR005121
- InterPro:   IPR002547 [H]

Pfam domain/function: PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 85137; Mature: 85137

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSINDLNVFVNTPKDIAKLCEDLSRLGLEVESCIPCIAPKNVVVGKILEKAPHKNAEK
CEEEEEEEEEEEECCHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHCCCCCCCC
LSVCQVDVGKEVLQIVCGAKNVAPNQFVPVALNGALIGSTTIAKTELRGVESHGMICSSI
EEEEEEHHHHHHHHHHHCCCCCCCCCEEEEEECCEEECCCCCHHHHHCCCCCCCEEEEEE
ELGFPKINDGILELDESVGELVLGKELNEYAPFNTHVLEISLTPNRGDCLSVLGIAREIS
ECCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHH
AFYHTPLKPIKALNFTPKSGLITLSAGENIESHLAYYLICNHSLKTPLNIKLSLAHNNAL
HHHCCCCCHHHHCCCCCCCCEEEEECCCCHHHHEEEEEEECCCCCCCCEEEEEEECCCCC
SENDLNNFIEFSTHFSGVIMNAYSLNTTPMDLSVKNDENNLESVYINHQKRSTIAIKHQV
CCCHHHHHHHHHHHHCEEEEEEEECCCCCCEEEECCCCCCCEEEEEECCCCCCHHHHHHH
QKDLSECLLLEASYTDPISLSLKLHALKDKTLQKDNALIYRSARGSNPNLSDGLNFLSAH
HHHHHHHHEEECCCCCCEEEEEEEEEHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHH
LKATILESKQTEHSLKDRTLTFQLEDITEILGLAVEKEKIQGILKNLGFKVSVKEPNSKP
HHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCC
QILEVIAPNFRHDIKTIQDIAEEILRFVGIDNLVSKPLHCVSSKNSNPNYDTHRFFENLK
CHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHCCCCCCCCCHHHHHHHHHH
HKALACGFKEVIHYVFYSKEKQQKLGFEVLEDPLELQNPITTELNTLRTSLVCGLLDASL
HHHHHHHHHHHHHHHHHCCHHHHHCCHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHH
RNKNLGFKSIALYEKGSVYNSKREEIQKLGFLISGLQKKESYPDTKGKAWDFYSFAECVS
CCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
KVIGDFSLEKLTTQTPINHPYQSAKIIQNHEIIGVIAKIHPKVIQELDLFESYYAEIDAF
HHHCCCHHHHHHCCCCCCCCCCHHHHHCCCCCEEEHHHHCHHHHHHHHHHHHHHHHHHHH
KLKRPAMLLKPFSIYPSSVRDLTLIIDENTAFSGIKKALKDAQIPNLSEILPLDIFKESN
HCCCCHHHCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCHHHHCCHHEEECCC
NSIALSVRCVIHSLEKTLNDEEVNSAVQKALEILEKEFNARLKG
CEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKLSINDLNVFVNTPKDIAKLCEDLSRLGLEVESCIPCIAPKNVVVGKILEKAPHKNAEK
CEEEEEEEEEEEECCHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHCCCCCCCC
LSVCQVDVGKEVLQIVCGAKNVAPNQFVPVALNGALIGSTTIAKTELRGVESHGMICSSI
EEEEEEHHHHHHHHHHHCCCCCCCCCEEEEEECCEEECCCCCHHHHHCCCCCCCEEEEEE
ELGFPKINDGILELDESVGELVLGKELNEYAPFNTHVLEISLTPNRGDCLSVLGIAREIS
ECCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHH
AFYHTPLKPIKALNFTPKSGLITLSAGENIESHLAYYLICNHSLKTPLNIKLSLAHNNAL
HHHCCCCCHHHHCCCCCCCCEEEEECCCCHHHHEEEEEEECCCCCCCCEEEEEEECCCCC
SENDLNNFIEFSTHFSGVIMNAYSLNTTPMDLSVKNDENNLESVYINHQKRSTIAIKHQV
CCCHHHHHHHHHHHHCEEEEEEEECCCCCCEEEECCCCCCCEEEEEECCCCCCHHHHHHH
QKDLSECLLLEASYTDPISLSLKLHALKDKTLQKDNALIYRSARGSNPNLSDGLNFLSAH
HHHHHHHHEEECCCCCCEEEEEEEEEHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHH
LKATILESKQTEHSLKDRTLTFQLEDITEILGLAVEKEKIQGILKNLGFKVSVKEPNSKP
HHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCC
QILEVIAPNFRHDIKTIQDIAEEILRFVGIDNLVSKPLHCVSSKNSNPNYDTHRFFENLK
CHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHCCCCCCCCCHHHHHHHHHH
HKALACGFKEVIHYVFYSKEKQQKLGFEVLEDPLELQNPITTELNTLRTSLVCGLLDASL
HHHHHHHHHHHHHHHHHCCHHHHHCCHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHH
RNKNLGFKSIALYEKGSVYNSKREEIQKLGFLISGLQKKESYPDTKGKAWDFYSFAECVS
CCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
KVIGDFSLEKLTTQTPINHPYQSAKIIQNHEIIGVIAKIHPKVIQELDLFESYYAEIDAF
HHHCCCHHHHHHCCCCCCCCCCHHHHHCCCCCEEEHHHHCHHHHHHHHHHHHHHHHHHHH
KLKRPAMLLKPFSIYPSSVRDLTLIIDENTAFSGIKKALKDAQIPNLSEILPLDIFKESN
HCCCCHHHCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCHHHHCCHHEEECCC
NSIALSVRCVIHSLEKTLNDEEVNSAVQKALEILEKEFNARLKG
CEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]