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Identifier: 15645029

GI number: 15645029

Start: 412036

End: 413325

Strand: Reverse

Name: Not Available

Synonym: HP0401

Alternate gene names: 15645029

Gene position: 413325-412036 (Counterclockwise)

Preceding gene: 15645030

Following gene: 15645028

Centisome position: 24.78

GC content: 39.53

Gene sequence:

>1290_bases
GTGATAGAGCTTGACATTAACGCTAGCGATAAATCGCTCTCACACAGAGCCGTTATTTTTAGCCTGCTCGCTCAAAAACC
TTGTTTCGTGCGGAATTTTTTAATGGGAGAAGATTGTTTAAGCTCTTTAGAAATCGCTCAAAATTTAGGGGCTAAAGTGG
AAAATACCGCCAAAAATTCTTTTAAAATCACACCCCCAACAACTATAAAGGAGCCTAACAAGATTTTAAATTGCAACAAT
TCTGGCACAACCATGCGTTTATACAGCGGGCTTTTAAGCGCTCAAAAAGGGCTTTTTGTTTTAAGCGGGGACAATTCCTT
AAACGCACGCCCCATGAAAAGAATCATTGAGCCTTTGAAGGCTTTTGGGGCAAAAATTTTAGGGAGAGAGGATAACCATT
TCGCCCCCTTAGTGATCTTAGGGAGTCCGTTAAAAGCTTGCCATTATGAAAGCCCTATCGCTTCAGCTCAAGTCAAAAGC
GCTTTTATTTTAAGCGCCTTACAAGCTCAAGGCGCAAGCACTTATAAAGAAAGCGAGCTTAGCCGTAACCACACAGAAAT
CATGCTTAAAAGTTTGGGAGCTGATATTCACAATCAAGACGGCGTTTTAAAAATTTCACCCCTAGAAAAACCCCTAGAAG
CCTTTGATTTTACGATAGCTAATGATCCGTCTAGCGCGTTTTTTTTCGCCCTCGCTTGCGCGATTACGCCAAAAAGCCGC
CTTCTTTTAAAAAATGTCTTGCTCAACCCCACTCGCATAGAAGCTTTTGAAGTTTTGAAAAAAATGGGTGCTTCCATAGA
GTATGCGATTCAGTCCAAAGATTTAGAAATGATTGGCGATATTTATGTAGAGCATGCCCCTTTAAAAGCGATCAATATTG
ATCAAAATATCGCCAGTCTTATTGATGAAATCCCCGCTTTAAGTATCGCTATGCTTTTTGCAAAAGGCAAAAGCATGGTT
AAAAACGCTAAAGATTTACGAGCTAAAGAAAGCGACAGGATTAAAGCGGTTGTTTCTAATTTCAAAGCTTTAGGGATTGA
GTGCGAAGAGTTTGAAGATGGGTTTTATGTAGAGGGATTAGAAGATATAAGCCCATTAAAACAGCGCTTTTCTAGGATTA
AGCCCCCCCTTATCAAAAGCTTCAATGACCACAGGATTGCGATGAGTTTTGCTGTTTTAACTTTAGCGTTGCCTTTAGAA
ATTGATAATTTAGAATGCGCAAACATTTCTTTCCCGCAATTCAAACACCTACTCAATCAATTCAAAAAAGGGAGTCTTAA
TGGAAATTAA

Upstream 100 bases:

>100_bases
CCTGAATGATGAAGAGGTCAATTCAGCCGTGCAAAAAGCACTTGAAATTTTAGAAAAAGAATTTAACGCCCGCCTTAAAG
GATAATATAAAGGATAATAT

Downstream 100 bases:

>100_bases
AATGGCTAAGGATTATGGTTTTTGTTTTGGCGTCAAAAGAGCGATACAAATCGCTGAAAAAAATCAAAACAGCTTGATTT
TTGGATCGCTCATTCATAAC

Product: 3-phosphoshikimate 1-carboxyvinyltransferase

Products: NA

Alternate protein names: 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MIELDINASDKSLSHRAVIFSLLAQKPCFVRNFLMGEDCLSSLEIAQNLGAKVENTAKNSFKITPPTTIKEPNKILNCNN
SGTTMRLYSGLLSAQKGLFVLSGDNSLNARPMKRIIEPLKAFGAKILGREDNHFAPLVILGSPLKACHYESPIASAQVKS
AFILSALQAQGASTYKESELSRNHTEIMLKSLGADIHNQDGVLKISPLEKPLEAFDFTIANDPSSAFFFALACAITPKSR
LLLKNVLLNPTRIEAFEVLKKMGASIEYAIQSKDLEMIGDIYVEHAPLKAINIDQNIASLIDEIPALSIAMLFAKGKSMV
KNAKDLRAKESDRIKAVVSNFKALGIECEEFEDGFYVEGLEDISPLKQRFSRIKPPLIKSFNDHRIAMSFAVLTLALPLE
IDNLECANISFPQFKHLLNQFKKGSLNGN

Sequences:

>Translated_429_residues
MIELDINASDKSLSHRAVIFSLLAQKPCFVRNFLMGEDCLSSLEIAQNLGAKVENTAKNSFKITPPTTIKEPNKILNCNN
SGTTMRLYSGLLSAQKGLFVLSGDNSLNARPMKRIIEPLKAFGAKILGREDNHFAPLVILGSPLKACHYESPIASAQVKS
AFILSALQAQGASTYKESELSRNHTEIMLKSLGADIHNQDGVLKISPLEKPLEAFDFTIANDPSSAFFFALACAITPKSR
LLLKNVLLNPTRIEAFEVLKKMGASIEYAIQSKDLEMIGDIYVEHAPLKAINIDQNIASLIDEIPALSIAMLFAKGKSMV
KNAKDLRAKESDRIKAVVSNFKALGIECEEFEDGFYVEGLEDISPLKQRFSRIKPPLIKSFNDHRIAMSFAVLTLALPLE
IDNLECANISFPQFKHLLNQFKKGSLNGN
>Mature_429_residues
MIELDINASDKSLSHRAVIFSLLAQKPCFVRNFLMGEDCLSSLEIAQNLGAKVENTAKNSFKITPPTTIKEPNKILNCNN
SGTTMRLYSGLLSAQKGLFVLSGDNSLNARPMKRIIEPLKAFGAKILGREDNHFAPLVILGSPLKACHYESPIASAQVKS
AFILSALQAQGASTYKESELSRNHTEIMLKSLGADIHNQDGVLKISPLEKPLEAFDFTIANDPSSAFFFALACAITPKSR
LLLKNVLLNPTRIEAFEVLKKMGASIEYAIQSKDLEMIGDIYVEHAPLKAINIDQNIASLIDEIPALSIAMLFAKGKSMV
KNAKDLRAKESDRIKAVVSNFKALGIECEEFEDGFYVEGLEDISPLKQRFSRIKPPLIKSFNDHRIAMSFAVLTLALPLE
IDNLECANISFPQFKHLLNQFKKGSLNGN

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; sixth step. [C]

COG id: COG0128

COG function: function code E; 5-enolpyruvylshikimate-3-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EPSP synthase family [H]

Homologues:

Organism=Escherichia coli, GI1787137, Length=354, Percent_Identity=27.4011299435028, Blast_Score=112, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6320332, Length=457, Percent_Identity=23.1947483588621, Blast_Score=89, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001986
- InterPro:   IPR006264
- InterPro:   IPR023193
- InterPro:   IPR013792 [H]

Pfam domain/function: PF00275 EPSP_synthase [H]

EC number: =2.5.1.19 [H]

Molecular weight: Translated: 47241; Mature: 47241

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: PS00104 EPSP_SYNTHASE_1 ; PS00885 EPSP_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIELDINASDKSLSHRAVIFSLLAQKPCFVRNFLMGEDCLSSLEIAQNLGAKVENTAKNS
CEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
FKITPPTTIKEPNKILNCNNSGTTMRLYSGLLSAQKGLFVLSGDNSLNARPMKRIIEPLK
EEECCCCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHH
AFGAKILGREDNHFAPLVILGSPLKACHYESPIASAQVKSAFILSALQAQGASTYKESEL
HHHHHHCCCCCCCCCCEEEECCCHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
SRNHTEIMLKSLGADIHNQDGVLKISPLEKPLEAFDFTIANDPSSAFFFALACAITPKSR
CCHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHCEEEECCCCHHHHHHHHHHCCCHHH
LLLKNVLLNPTRIEAFEVLKKMGASIEYAIQSKDLEMIGDIYVEHAPLKAINIDQNIASL
HHHHHHHCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCCEEEECCHHHHHH
IDEIPALSIAMLFAKGKSMVKNAKDLRAKESDRIKAVVSNFKALGIECEEFEDGFYVEGL
HHHCCHHHHHHHHHHCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHCCCCCEECCH
EDISPLKQRFSRIKPPLIKSFNDHRIAMSFAVLTLALPLEIDNLECANISFPQFKHLLNQ
HHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHCEEECCCCCCCCCCHHHHHHHHH
FKKGSLNGN
HHCCCCCCC
>Mature Secondary Structure
MIELDINASDKSLSHRAVIFSLLAQKPCFVRNFLMGEDCLSSLEIAQNLGAKVENTAKNS
CEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
FKITPPTTIKEPNKILNCNNSGTTMRLYSGLLSAQKGLFVLSGDNSLNARPMKRIIEPLK
EEECCCCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHH
AFGAKILGREDNHFAPLVILGSPLKACHYESPIASAQVKSAFILSALQAQGASTYKESEL
HHHHHHCCCCCCCCCCEEEECCCHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
SRNHTEIMLKSLGADIHNQDGVLKISPLEKPLEAFDFTIANDPSSAFFFALACAITPKSR
CCHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHCEEEECCCCHHHHHHHHHHCCCHHH
LLLKNVLLNPTRIEAFEVLKKMGASIEYAIQSKDLEMIGDIYVEHAPLKAINIDQNIASL
HHHHHHHCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCCEEEECCHHHHHH
IDEIPALSIAMLFAKGKSMVKNAKDLRAKESDRIKAVVSNFKALGIECEEFEDGFYVEGL
HHHCCHHHHHHHHHHCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHCCCCCEECCH
EDISPLKQRFSRIKPPLIKSFNDHRIAMSFAVLTLALPLEIDNLECANISFPQFKHLLNQ
HHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHCEEECCCCCCCCCCHHHHHHHHH
FKKGSLNGN
HHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA