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Identifier: 15645016

GI number: 15645016

Start: 397646

End: 398377

Strand: Reverse

Name: Not Available

Synonym: HP0388

Alternate gene names: 15645016

Gene position: 398377-397646 (Counterclockwise)

Preceding gene: 15645017

Following gene: 15645012

Centisome position: 23.89

GC content: 38.11

Gene sequence:

>732_bases
ATGAAAGACACTCTGTTTAATGAATCCCTAAACAAACGCTTTTGTTTTGATGAAAAAGTCGCCCATGTTTTTGATGACAT
GCTGGAGCGATCTATCCCCTATTATCATGAAATGTTGAATCTAGGGGCGTATTTTATCGCTCAAAATTTAAAAGAAAATA
TTTATCCTAAGTCCTTGCCTAAGCCCTTGATTTATGATTTGGGCTGTTCTACCGGGAACTTTTTTATCGCGCTTAACCGA
CAAATCCAACAAGAGATTGAGCTTGTAGGGATTGATAATTCCATGCCCATGCTCAAAAAAGCGCAAGAAAAATTAAAAGA
TTTTAACAATGCCCGTTTTGAATGCATGGATTTTTTAGAGGTTGAGTTTAAAGAAGCGAGCGCGTTTTCATTGCTTTTTG
TGTTGCAATTTGTCCGCCCCATGCAAAGAGAGGTGTTGCTCAAAAAGATTTATAACAGCCTTGCGTTGAATGGGGTTTTA
TTGGTGGGCGAAAAGATCATGAGCGAAGATCGGATATTAGACAAGCAAATGATAGAGCTATACTACCTTTATAAACAAAA
TCAAGGCTATAGCCACAATGAAATCGCTTTCAAAAGGGAAGCGTTAGAAAATGTACTTGTGCCTTATAGTTTGAAAGAAA
ATGTCGCACTTTTAGAAAGCGTGGGGTTTAAGCATGTGGAAGCGCTGTTTAAATGGGTGAATTTCACGCTGTTGGTCGCC
AGAAAAACTTAA

Upstream 100 bases:

>100_bases
ATCAGTGGATTACTACATCAATGAGTTGGTGCATAAAAAAGCTTAAGCGTTATTTTGCATAGAGTGGTTTAAAGGTTTTT
AAACCACGCTTCTTTGTTGA

Downstream 100 bases:

>100_bases
GTTTTTTGAAATAATATATAAAACATGAGTTTTTTAAAAAATATCCACAGGATCCATGTTCACGCTGCAAGGGATACTAG
GGGCGGATTTTAAAAACGCA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MKDTLFNESLNKRFCFDEKVAHVFDDMLERSIPYYHEMLNLGAYFIAQNLKENIYPKSLPKPLIYDLGCSTGNFFIALNR
QIQQEIELVGIDNSMPMLKKAQEKLKDFNNARFECMDFLEVEFKEASAFSLLFVLQFVRPMQREVLLKKIYNSLALNGVL
LVGEKIMSEDRILDKQMIELYYLYKQNQGYSHNEIAFKREALENVLVPYSLKENVALLESVGFKHVEALFKWVNFTLLVA
RKT

Sequences:

>Translated_243_residues
MKDTLFNESLNKRFCFDEKVAHVFDDMLERSIPYYHEMLNLGAYFIAQNLKENIYPKSLPKPLIYDLGCSTGNFFIALNR
QIQQEIELVGIDNSMPMLKKAQEKLKDFNNARFECMDFLEVEFKEASAFSLLFVLQFVRPMQREVLLKKIYNSLALNGVL
LVGEKIMSEDRILDKQMIELYYLYKQNQGYSHNEIAFKREALENVLVPYSLKENVALLESVGFKHVEALFKWVNFTLLVA
RKT
>Mature_243_residues
MKDTLFNESLNKRFCFDEKVAHVFDDMLERSIPYYHEMLNLGAYFIAQNLKENIYPKSLPKPLIYDLGCSTGNFFIALNR
QIQQEIELVGIDNSMPMLKKAQEKLKDFNNARFECMDFLEVEFKEASAFSLLFVLQFVRPMQREVLLKKIYNSLALNGVL
LVGEKIMSEDRILDKQMIELYYLYKQNQGYSHNEIAFKREALENVLVPYSLKENVALLESVGFKHVEALFKWVNFTLLVA
RKT

Specific function: Catalyzes the conversion of 5-methoxyuridine (mo5U) to uridine-5-oxyacetic acid (cmo5U) at position 34 in tRNA. May also participate in the methylation of uridine-5-oxyacetic acid (cmo5U) to uridine-5-oxyacetic acid methyl ester (mcmo5U) [H]

COG id: COG0500

COG function: function code QR; SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. CmoA family [H]

Homologues:

Organism=Escherichia coli, GI1788177, Length=250, Percent_Identity=32.4, Blast_Score=135, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013217
- InterPro:   IPR005271 [H]

Pfam domain/function: PF08242 Methyltransf_12 [H]

EC number: NA

Molecular weight: Translated: 28483; Mature: 28483

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDTLFNESLNKRFCFDEKVAHVFDDMLERSIPYYHEMLNLGAYFIAQNLKENIYPKSLP
CCCCHHCHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
KPLIYDLGCSTGNFFIALNRQIQQEIELVGIDNSMPMLKKAQEKLKDFNNARFECMDFLE
CCCEEECCCCCCCEEEEECHHHHHHHHEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHH
VEFKEASAFSLLFVLQFVRPMQREVLLKKIYNSLALNGVLLVGEKIMSEDRILDKQMIEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
YYLYKQNQGYSHNEIAFKREALENVLVPYSLKENVALLESVGFKHVEALFKWVNFTLLVA
HHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHEEEEE
RKT
ECC
>Mature Secondary Structure
MKDTLFNESLNKRFCFDEKVAHVFDDMLERSIPYYHEMLNLGAYFIAQNLKENIYPKSLP
CCCCHHCHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
KPLIYDLGCSTGNFFIALNRQIQQEIELVGIDNSMPMLKKAQEKLKDFNNARFECMDFLE
CCCEEECCCCCCCEEEEECHHHHHHHHEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHH
VEFKEASAFSLLFVLQFVRPMQREVLLKKIYNSLALNGVLLVGEKIMSEDRILDKQMIEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
YYLYKQNQGYSHNEIAFKREALENVLVPYSLKENVALLESVGFKHVEALFKWVNFTLLVA
HHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHEEEEE
RKT
ECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA