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The map label for this gene is tgt

Identifier: 15644909

GI number: 15644909

Start: 288852

End: 289967

Strand: Reverse

Name: tgt

Synonym: HP0281

Alternate gene names: 15644909

Gene position: 289967-288852 (Counterclockwise)

Preceding gene: 15644923

Following gene: 15644889

Centisome position: 17.39

GC content: 43.91

Gene sequence:

>1116_bases
ATGGATTTTCAACTCCAAGCGACTGACAATAACGCGCGAGCTGGTCTTTTAAATTTAGCCCATTCTCAAGTGGCAACGCC
TGTTTTTATGCCCGTAGGCACGCAAGGCTGCATCAAATCTTTAGACGCTACAGATGCGCAAGAAATTTTAGGCGCTAAAC
TCATTTTAGCCAACACCTATCACATGTATTTAAGGCCGGGTGAAAAGGTCGTTGAGGAGTTAGGGGGCTTGCATCGTTTC
GCTCAATTTTATGGGAGTTTTTTAACCGATAGTGGAGGGTTTCAAGCCTTTAGTTTGAGCGATAATGTCAAATTGCAAGA
AGATGGGATCGTTTTTAAATCCCATATTGATGGGAGCAAGCATCTATTCACGCCCGCTAAAGTTTTGGACATTCAATATT
CTTTGAATAGCGATATTATGATGGTTTTAGACGATTTAGTGGGCTTGCCCGCTCCCTTAAAACGCCTTGAAGAATCCATT
AAAAGAAGTGCTAAATGGGCGAATATGAGCCTAGAATACCACAAAGAAAAAAACCGCCCGAGCAACAACCTTTTTGCCAT
TATCCAGGGCGGGACGCATTTGAAAATGCGCAGCCTTAGCGTGGGATTAACGCATGAGGGTTTTGATGGCTACGCTATAG
GCGGTTTAGCGGTGGGGGAAAGCGCTGATGAAATGCTAGAAACCATCGCGCACACCGCCCCCTTGCTCCCCAAAGACAAG
CCTCGCTACTTAATGGGCGTAGGCACGCCTGAAAATATCCTAGACGCTATCAGTTTGGGGGTGGATATGTTTGATTGCGT
GATGCCCACCAGAAACGCCAGAAACGCCACCCTTTTCACGCATTCTGGCAAAATTTCTATCAAAAACGCGCCCTATAAAT
TGGATAATACCCCTATTGAAGAAAATTGCGCATGTTATGCTTGCAAACGCTATTCTAAAGCCTATTTGCACCATTTATTT
AGGGCTAAAGAACTCACTTACGCTCGTTTGGCCAGCTTGCACAATTTGCATTTTTATTTAGAGCTGGTGAAGAACGCCAG
AAACGCCATTTTAGAAAAGCGGTTTTTGAGTTTTAAAAAAGAATTTTTGGAGAAATACAACTCCCGCTCTCATTGA

Upstream 100 bases:

>100_bases
TTTTTTGCTACAATGCCATCTAAAATAAGGGCGATTTTTTTCAATTCCAAACTTCCTAAAAAATGATTACGCCATTATAT
CCAAGATTAAGGCTTAAACG

Downstream 100 bases:

>100_bases
ATGATGGAATGCAAAAATACTAAAAAGCGTTTTTTACCATCAATAAAAGTTTTCTTAAAAATAAGGCTTTAGTTTTTAAT
TTTTGATTAAAACAAAACCC

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MDFQLQATDNNARAGLLNLAHSQVATPVFMPVGTQGCIKSLDATDAQEILGAKLILANTYHMYLRPGEKVVEELGGLHRF
AQFYGSFLTDSGGFQAFSLSDNVKLQEDGIVFKSHIDGSKHLFTPAKVLDIQYSLNSDIMMVLDDLVGLPAPLKRLEESI
KRSAKWANMSLEYHKEKNRPSNNLFAIIQGGTHLKMRSLSVGLTHEGFDGYAIGGLAVGESADEMLETIAHTAPLLPKDK
PRYLMGVGTPENILDAISLGVDMFDCVMPTRNARNATLFTHSGKISIKNAPYKLDNTPIEENCACYACKRYSKAYLHHLF
RAKELTYARLASLHNLHFYLELVKNARNAILEKRFLSFKKEFLEKYNSRSH

Sequences:

>Translated_371_residues
MDFQLQATDNNARAGLLNLAHSQVATPVFMPVGTQGCIKSLDATDAQEILGAKLILANTYHMYLRPGEKVVEELGGLHRF
AQFYGSFLTDSGGFQAFSLSDNVKLQEDGIVFKSHIDGSKHLFTPAKVLDIQYSLNSDIMMVLDDLVGLPAPLKRLEESI
KRSAKWANMSLEYHKEKNRPSNNLFAIIQGGTHLKMRSLSVGLTHEGFDGYAIGGLAVGESADEMLETIAHTAPLLPKDK
PRYLMGVGTPENILDAISLGVDMFDCVMPTRNARNATLFTHSGKISIKNAPYKLDNTPIEENCACYACKRYSKAYLHHLF
RAKELTYARLASLHNLHFYLELVKNARNAILEKRFLSFKKEFLEKYNSRSH
>Mature_371_residues
MDFQLQATDNNARAGLLNLAHSQVATPVFMPVGTQGCIKSLDATDAQEILGAKLILANTYHMYLRPGEKVVEELGGLHRF
AQFYGSFLTDSGGFQAFSLSDNVKLQEDGIVFKSHIDGSKHLFTPAKVLDIQYSLNSDIMMVLDDLVGLPAPLKRLEESI
KRSAKWANMSLEYHKEKNRPSNNLFAIIQGGTHLKMRSLSVGLTHEGFDGYAIGGLAVGESADEMLETIAHTAPLLPKDK
PRYLMGVGTPENILDAISLGVDMFDCVMPTRNARNATLFTHSGKISIKNAPYKLDNTPIEENCACYACKRYSKAYLHHLF
RAKELTYARLASLHNLHFYLELVKNARNAILEKRFLSFKKEFLEKYNSRSH

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=364, Percent_Identity=39.8351648351648, Blast_Score=293, Evalue=3e-79,
Organism=Escherichia coli, GI1786607, Length=366, Percent_Identity=46.448087431694, Blast_Score=349, Evalue=1e-97,
Organism=Caenorhabditis elegans, GI17544680, Length=368, Percent_Identity=38.0434782608696, Blast_Score=248, Evalue=5e-66,
Organism=Caenorhabditis elegans, GI25146502, Length=171, Percent_Identity=29.2397660818713, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19920510, Length=362, Percent_Identity=40.3314917127072, Blast_Score=297, Evalue=7e-81,
Organism=Drosophila melanogaster, GI21355311, Length=255, Percent_Identity=27.843137254902, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 41416; Mature: 41416

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFQLQATDNNARAGLLNLAHSQVATPVFMPVGTQGCIKSLDATDAQEILGAKLILANTY
CCEEEEECCCCCCHHHHHHHHHHHCCCEEEECCCHHHHHHCCCCCHHHHHCCEEEEECCE
HMYLRPGEKVVEELGGLHRFAQFYGSFLTDSGGFQAFSLSDNVKLQEDGIVFKSHIDGSK
EEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEECCCEEEEECCCCCC
HLFTPAKVLDIQYSLNSDIMMVLDDLVGLPAPLKRLEESIKRSAKWANMSLEYHKEKNRP
CEECCCEEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEHHHCCCC
SNNLFAIIQGGTHLKMRSLSVGLTHEGFDGYAIGGLAVGESADEMLETIAHTAPLLPKDK
CCCEEEEEECCCEEEEEEEEEECEECCCCCEEECCEEECCCHHHHHHHHHHHCCCCCCCC
PRYLMGVGTPENILDAISLGVDMFDCVMPTRNARNATLFTHSGKISIKNAPYKLDNTPIE
CCEEEECCCHHHHHHHHHHCHHHHHHHCCCCCCCCEEEEEECCEEEECCCCEECCCCCCC
ENCACYACKRYSKAYLHHLFRAKELTYARLASLHNLHFYLELVKNARNAILEKRFLSFKK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EFLEKYNSRSH
HHHHHHCCCCC
>Mature Secondary Structure
MDFQLQATDNNARAGLLNLAHSQVATPVFMPVGTQGCIKSLDATDAQEILGAKLILANTY
CCEEEEECCCCCCHHHHHHHHHHHCCCEEEECCCHHHHHHCCCCCHHHHHCCEEEEECCE
HMYLRPGEKVVEELGGLHRFAQFYGSFLTDSGGFQAFSLSDNVKLQEDGIVFKSHIDGSK
EEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEECCCEEEEECCCCCC
HLFTPAKVLDIQYSLNSDIMMVLDDLVGLPAPLKRLEESIKRSAKWANMSLEYHKEKNRP
CEECCCEEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEHHHCCCC
SNNLFAIIQGGTHLKMRSLSVGLTHEGFDGYAIGGLAVGESADEMLETIAHTAPLLPKDK
CCCEEEEEECCCEEEEEEEEEECEECCCCCEEECCEEECCCHHHHHHHHHHHCCCCCCCC
PRYLMGVGTPENILDAISLGVDMFDCVMPTRNARNATLFTHSGKISIKNAPYKLDNTPIE
CCEEEECCCHHHHHHHHHHCHHHHHHHCCCCCCCCEEEEEECCEEEECCCCEECCCCCCC
ENCACYACKRYSKAYLHHLFRAKELTYARLASLHNLHFYLELVKNARNAILEKRFLSFKK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EFLEKYNSRSH
HHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA