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Identifier: 15644740

GI number: 15644740

Start: 118254

End: 118823

Strand: Reverse

Name: Not Available

Synonym: HP0110

Alternate gene names: 15644740

Gene position: 118823-118254 (Counterclockwise)

Preceding gene: 15644741

Following gene: 15644739

Centisome position: 7.12

GC content: 41.58

Gene sequence:

>570_bases
ATGAAAGATGAACACAACCAAGAACACGATCATTTAAGCCAAAAAGAGCCAGAGTTTTGCGAAAAGGCTTGCAAAGAACA
ACAATATGAAGAAAAGCAAGAAGCGGGCGAAAAAGAAGGCGAGATCAAGGAAGATTTTGAGCTTAAATATAAAGAAATGC
ACGAAAAATACTTAAGAGTGCATGCGGATTTTGAAAACGTGAAAAAGCGCTTAGAAAGAGACAAGAGCATGGCACTAGAG
TATGCGTATGAAAAGATCGCATTGGATCTATTGCCGGTGATTGATGCACTTCTTGGGGCTCATAAAAGCGCGGCTGAAGA
GGATAAAGAGAGCGCTTTAACCAAGGGCTTGGAGCTTACGATGGAAAAGTTGCATGAAGTTTTGGCAAGGCATGGCATTG
AGGGGATTGAATGCTTAGAAGAATTTGATCCCCATTTCCACAATGCGATCATGCAAGTCAAAAGCGAAGAAAAAGAAAAC
GGGAAAATCGTGCAGGTTTTGCAACAGGGTTACAAGTATAAGGGTAGGGTTTTGAGGCCGGCAATGGTGAGCATTGCTAA
AAACGATTAA

Upstream 100 bases:

>100_bases
GCCCTGTAGAATTTCAAAACAAGCGGATGCAACTGCTTTGCGTGGGGAAACTAGAATGCGATTATGAAGGGTTTTTTCAA
ACGATTTCTGAGGAGGAATA

Downstream 100 bases:

>100_bases
AACATTTATTTTAAAAGAAAGGATAACGCATGGGAAAAGTTATTGGAATTGATTTAGGGACAACCAACTCCGCAATGGCG
GTTTATGAAGGCAATGAAGC

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 189; Mature: 189

Protein sequence:

>189_residues
MKDEHNQEHDHLSQKEPEFCEKACKEQQYEEKQEAGEKEGEIKEDFELKYKEMHEKYLRVHADFENVKKRLERDKSMALE
YAYEKIALDLLPVIDALLGAHKSAAEEDKESALTKGLELTMEKLHEVLARHGIEGIECLEEFDPHFHNAIMQVKSEEKEN
GKIVQVLQQGYKYKGRVLRPAMVSIAKND

Sequences:

>Translated_189_residues
MKDEHNQEHDHLSQKEPEFCEKACKEQQYEEKQEAGEKEGEIKEDFELKYKEMHEKYLRVHADFENVKKRLERDKSMALE
YAYEKIALDLLPVIDALLGAHKSAAEEDKESALTKGLELTMEKLHEVLARHGIEGIECLEEFDPHFHNAIMQVKSEEKEN
GKIVQVLQQGYKYKGRVLRPAMVSIAKND
>Mature_189_residues
MKDEHNQEHDHLSQKEPEFCEKACKEQQYEEKQEAGEKEGEIKEDFELKYKEMHEKYLRVHADFENVKKRLERDKSMALE
YAYEKIALDLLPVIDALLGAHKSAAEEDKESALTKGLELTMEKLHEVLARHGIEGIECLEEFDPHFHNAIMQVKSEEKEN
GKIVQVLQQGYKYKGRVLRPAMVSIAKND

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI24308295, Length=170, Percent_Identity=32.3529411764706, Blast_Score=73, Evalue=1e-13,
Organism=Escherichia coli, GI1788967, Length=133, Percent_Identity=39.0977443609023, Blast_Score=97, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17552458, Length=152, Percent_Identity=31.5789473684211, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324806, Length=181, Percent_Identity=32.5966850828729, Blast_Score=86, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24653432, Length=143, Percent_Identity=35.6643356643357, Blast_Score=80, Evalue=8e-16,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 22040; Mature: 22040

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDEHNQEHDHLSQKEPEFCEKACKEQQYEEKQEAGEKEGEIKEDFELKYKEMHEKYLRV
CCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
HADFENVKKRLERDKSMALEYAYEKIALDLLPVIDALLGAHKSAAEEDKESALTKGLELT
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
MEKLHEVLARHGIEGIECLEEFDPHFHNAIMQVKSEEKENGKIVQVLQQGYKYKGRVLRP
HHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHH
AMVSIAKND
HHHHHHCCC
>Mature Secondary Structure
MKDEHNQEHDHLSQKEPEFCEKACKEQQYEEKQEAGEKEGEIKEDFELKYKEMHEKYLRV
CCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
HADFENVKKRLERDKSMALEYAYEKIALDLLPVIDALLGAHKSAAEEDKESALTKGLELT
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
MEKLHEVLARHGIEGIECLEEFDPHFHNAIMQVKSEEKENGKIVQVLQQGYKYKGRVLRP
HHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHH
AMVSIAKND
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA