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The map label for this gene is bioB

Identifier: 15618952

GI number: 15618952

Start: 1197696

End: 1198691

Strand: Reverse

Name: bioB

Synonym: CPn1044

Alternate gene names: 15618952

Gene position: 1198691-1197696 (Counterclockwise)

Preceding gene: 15618953

Following gene: 15618951

Centisome position: 97.44

GC content: 43.78

Gene sequence:

>996_bases
ATGCGTGAAGAAACTGTATCCTGGTCATTAGAAGACATCCGCGAAATTTATCACACTCCCGTATTTGAGCTGATTCACAA
AGCCAATGCCATATTGCGTAGTAATTTCCTCCATTCAGAACTGCAGACTTGCTATCTGATTTCGATTAAAACTGGTGGAT
GCGTTGAAGATTGCGCCTACTGTGCCCAATCTTCCCGCTATCATACCCACGTCACACCAGAACCTATGATGAAAATTGTA
GACGTTGTGGAAAGGGCAAAACGTGCTGTAGAGCTAGGCGCCACTCGTGTGTGTCTTGGGGCTGCCTGGCGCAATGCTAA
GGACGATCGATACTTTGATAGAGTCCTCGCTATGGTGAAAAGTATCACAGATCTCGGAGCCGAGGTTTGTTGTGCTTTAG
GCATGCTCTCCGAAGAGCAAGCTAAAAAACTGTATGATGCAGGACTTTATGCCTACAATCATAATTTAGACTCTTCTCCG
GAATTCTATGAAACTATAATCACAACACGTTCTTATGAAGATCGCCTCAACACTCTTGATGTAGTAAATAAATCTGGCAT
TAGTACATGCTGCGGTGGTATTGTAGGTATGGGAGAATCTGAAGAAGACCGTATAAAGCTTCTTCATGTTCTTGCAACAA
GAGATCATATCCCAGAATCCGTACCTGTAAATTTACTTTGGCCGATTGACGGCACGCCTTTGCAAGACCAGCCTCCGATT
TCTTTCTGGGAAGTCTTGCGAACCATAGCAACGGCACGGGTTGTTTTCCCCAGATCCATGGTACGACTTGCTGCAGGACG
CGCTTTCCTCACAGTAGAACAACAAACCTTATGTTTTCTAGCCGGTGCCAACTCCATATTCTATGGAGATAAACTGTTGA
CTGTAGAAAACAATGATATAGATGAAGATGCTGAAATGATCAAACTTTTAGGCTTAATCCCTCGCCCTTCATTTGGAATA
GAAAGAGGTAACCCATGTTATGCCAACAATTCCTAA

Upstream 100 bases:

>100_bases
ATAAAAAATAGGAACCAAAAATTGGTTCCTATTGACTTTCTCTAAATTCCATGTAAATATCTTAGGCTCGGTCGTTGAAT
TTACCCCCTAGGCAAAACAC

Downstream 100 bases:

>100_bases
TTGAGGCTTTGGCAAGACGTAAGTCCAAACATACCTACCGCTCGCTAAGCTTAAACTCTCATCTCATTGATTTTACCTCT
AATGATTATCTTGGATTTGC

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MREETVSWSLEDIREIYHTPVFELIHKANAILRSNFLHSELQTCYLISIKTGGCVEDCAYCAQSSRYHTHVTPEPMMKIV
DVVERAKRAVELGATRVCLGAAWRNAKDDRYFDRVLAMVKSITDLGAEVCCALGMLSEEQAKKLYDAGLYAYNHNLDSSP
EFYETIITTRSYEDRLNTLDVVNKSGISTCCGGIVGMGESEEDRIKLLHVLATRDHIPESVPVNLLWPIDGTPLQDQPPI
SFWEVLRTIATARVVFPRSMVRLAAGRAFLTVEQQTLCFLAGANSIFYGDKLLTVENNDIDEDAEMIKLLGLIPRPSFGI
ERGNPCYANNS

Sequences:

>Translated_331_residues
MREETVSWSLEDIREIYHTPVFELIHKANAILRSNFLHSELQTCYLISIKTGGCVEDCAYCAQSSRYHTHVTPEPMMKIV
DVVERAKRAVELGATRVCLGAAWRNAKDDRYFDRVLAMVKSITDLGAEVCCALGMLSEEQAKKLYDAGLYAYNHNLDSSP
EFYETIITTRSYEDRLNTLDVVNKSGISTCCGGIVGMGESEEDRIKLLHVLATRDHIPESVPVNLLWPIDGTPLQDQPPI
SFWEVLRTIATARVVFPRSMVRLAAGRAFLTVEQQTLCFLAGANSIFYGDKLLTVENNDIDEDAEMIKLLGLIPRPSFGI
ERGNPCYANNS
>Mature_331_residues
MREETVSWSLEDIREIYHTPVFELIHKANAILRSNFLHSELQTCYLISIKTGGCVEDCAYCAQSSRYHTHVTPEPMMKIV
DVVERAKRAVELGATRVCLGAAWRNAKDDRYFDRVLAMVKSITDLGAEVCCALGMLSEEQAKKLYDAGLYAYNHNLDSSP
EFYETIITTRSYEDRLNTLDVVNKSGISTCCGGIVGMGESEEDRIKLLHVLATRDHIPESVPVNLLWPIDGTPLQDQPPI
SFWEVLRTIATARVVFPRSMVRLAAGRAFLTVEQQTLCFLAGANSIFYGDKLLTVENNDIDEDAEMIKLLGLIPRPSFGI
ERGNPCYANNS

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=310, Percent_Identity=47.4193548387097, Blast_Score=306, Evalue=1e-84,
Organism=Saccharomyces cerevisiae, GI6321725, Length=327, Percent_Identity=46.7889908256881, Blast_Score=315, Evalue=8e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_CHLPN (Q9Z6L5)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   AE009440
- EMBL:   BA000008
- PIR:   A86621
- PIR:   H72004
- RefSeq:   NP_225238.1
- RefSeq:   NP_301099.1
- RefSeq:   NP_445347.1
- RefSeq:   NP_877356.1
- HSSP:   P12996
- ProteinModelPortal:   Q9Z6L5
- SMR:   Q9Z6L5
- GeneID:   1467763
- GeneID:   895651
- GeneID:   919818
- GeneID:   962741
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0808
- KEGG:   cpn:CPn1044
- KEGG:   cpt:CpB1084
- TIGR:   CP_0808
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- PhylomeDB:   Q9Z6L5
- ProtClustDB:   CLSK2300100
- BioCyc:   CPNE115711:CP_0808-MONOMER
- BioCyc:   CPNE115713:CPN1044-MONOMER
- BioCyc:   CPNE138677:CPJ1044-MONOMER
- BioCyc:   CPNE182082:CPB1084-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 37076; Mature: 37076

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREETVSWSLEDIREIYHTPVFELIHKANAILRSNFLHSELQTCYLISIKTGGCVEDCAY
CCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCHHHHHH
CAQSSRYHTHVTPEPMMKIVDVVERAKRAVELGATRVCLGAAWRNAKDDRYFDRVLAMVK
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHH
SITDLGAEVCCALGMLSEEQAKKLYDAGLYAYNHNLDSSPEFYETIITTRSYEDRLNTLD
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHCCCHHHHHHHHH
VVNKSGISTCCGGIVGMGESEEDRIKLLHVLATRDHIPESVPVNLLWPIDGTPLQDQPPI
HHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCC
SFWEVLRTIATARVVFPRSMVRLAAGRAFLTVEQQTLCFLAGANSIFYGDKLLTVENNDI
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEECCCCEEECCEEEEECCCCC
DEDAEMIKLLGLIPRPSFGIERGNPCYANNS
CHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MREETVSWSLEDIREIYHTPVFELIHKANAILRSNFLHSELQTCYLISIKTGGCVEDCAY
CCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCHHHHHH
CAQSSRYHTHVTPEPMMKIVDVVERAKRAVELGATRVCLGAAWRNAKDDRYFDRVLAMVK
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHH
SITDLGAEVCCALGMLSEEQAKKLYDAGLYAYNHNLDSSPEFYETIITTRSYEDRLNTLD
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHCCCHHHHHHHHH
VVNKSGISTCCGGIVGMGESEEDRIKLLHVLATRDHIPESVPVNLLWPIDGTPLQDQPPI
HHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCC
SFWEVLRTIATARVVFPRSMVRLAAGRAFLTVEQQTLCFLAGANSIFYGDKLLTVENNDI
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEECCCCEEECCEEEEECCCCC
DEDAEMIKLLGLIPRPSFGIERGNPCYANNS
CHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10871362; 10684935