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The map label for this gene is eno

Identifier: 15618709

GI number: 15618709

Start: 905246

End: 906532

Strand: Reverse

Name: eno

Synonym: CPn0800

Alternate gene names: 15618709

Gene position: 906532-905246 (Counterclockwise)

Preceding gene: 15618710

Following gene: 15618708

Centisome position: 73.69

GC content: 42.89

Gene sequence:

>1287_bases
ATGTTTGAAGCTGTCATTGCCGATATCCAGGCTAGGGAAATCTTGGATTCTCGCGGGTATCCCACTTTACATGTTAAAGT
AACCACTAGCACAGGTTCTGTTGGAGAAGCTCGGGTTCCTTCAGGAGCATCCACAGGGAAAAAAGAAGCCTTAGAGTTTC
GTGATACAGATTCTCCTCGTTATCAAGGCAAAGGGGTTTTGCAAGCTGTAAAAAACGTAAAAGAAATTCTTTTTCCCCTC
GTCAAGGGATGTAGTGTTTATGAGCAATCCTTAATTGATTCTCTGATGATGGATTCTGACGGCTCTCCGAACAAAGAAAC
TCTAGGGGCCAATGCTATTTTAGGAGTCTCTCTAGCTACAGCACATGCAGCAGCAGCAACACTACGCAGACCTCTGTATC
GTTATTTAGGAGGGTGTTTTGCCTGCAGTCTTCCCTGTCCTATGATGAATCTGATCAATGGAGGCATGCATGCCGATAAC
GGCTTGGAGTTCCAAGAATTTATGATCCGTCCTATTGGAGCCTCTTCCATCAAAGAAGCTGTCAACATGGGTGCTGACGT
TTTTCATACTTTGAAAAAATTACTCCATGAAAGAGGCTTATCTACTGGAGTGGGTGACGAAGGAGGCTTCGCCCCGAATC
TTGCTTCTAATGAAGAAGCTCTAGAGCTCCTATTGCTGGCTATTGAAAAAGCAGGCTTTACTCCAGGAAAAGATATATCG
CTAGCCTTAGACTGCGCAGCATCCTCATTCTATAACGTAAAAACAGGCACGTATGATGGGAGGCACTATGAAGAGCAAAT
CGCAATCCTTTCTAATTTATGTGATCGCTATCCTATAGACTCCATAGAAGATGGTCTTGCTGAAGAAGACTATGACGGGT
GGGCCTTGTTAACTGAAGTTCTTGGAGAAAAAGTACAGATTGTGGGTGATGACCTATTTGTTACAAATCCGGAATTAATA
TTAGAGGGTATTAGCAATGGATTAGCGAACTCTGTGTTGATTAAACCAAATCAGATAGGGACGCTTACTGAAACAGTGTA
TGCTATCAAGCTTGCGCAAATGGCTGGCTATACTACAATTATTTCTCATCGCTCAGGAGAAACTACGGACACTACGATTG
CAGATCTTGCTGTTGCCTTCAACGCCGGTCAAATCAAAACAGGCTCTTTATCACGTTCTGAGCGTGTTGCAAAATACAAT
AGACTCATGGAAATTGAAGAAGAGCTTGGATCCGAAGCAATTTTCACAGATTCTAATGTATTTTCTTACGAGGATTCTGA
GGAATAG

Upstream 100 bases:

>100_bases
TTTTAGAATACATATCTACAGTTCAAGAAGCAGAGCAGGATCAGTAGGTTCACATGCTATTGAGCTCTTAGCAAAGTCTT
AAAGAAAAGAGATAAAAATT

Downstream 100 bases:

>100_bases
AGGGATTTTCTCTATTTCTATCTTATCTCCAAATCAAGAATACATCGCAGATCTATGGGCCTTATCTTTCTGATCTGCAA
TGTGTTCTTTATTTCCCTCT

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MFEAVIADIQAREILDSRGYPTLHVKVTTSTGSVGEARVPSGASTGKKEALEFRDTDSPRYQGKGVLQAVKNVKEILFPL
VKGCSVYEQSLIDSLMMDSDGSPNKETLGANAILGVSLATAHAAAATLRRPLYRYLGGCFACSLPCPMMNLINGGMHADN
GLEFQEFMIRPIGASSIKEAVNMGADVFHTLKKLLHERGLSTGVGDEGGFAPNLASNEEALELLLLAIEKAGFTPGKDIS
LALDCAASSFYNVKTGTYDGRHYEEQIAILSNLCDRYPIDSIEDGLAEEDYDGWALLTEVLGEKVQIVGDDLFVTNPELI
LEGISNGLANSVLIKPNQIGTLTETVYAIKLAQMAGYTTIISHRSGETTDTTIADLAVAFNAGQIKTGSLSRSERVAKYN
RLMEIEEELGSEAIFTDSNVFSYEDSEE

Sequences:

>Translated_428_residues
MFEAVIADIQAREILDSRGYPTLHVKVTTSTGSVGEARVPSGASTGKKEALEFRDTDSPRYQGKGVLQAVKNVKEILFPL
VKGCSVYEQSLIDSLMMDSDGSPNKETLGANAILGVSLATAHAAAATLRRPLYRYLGGCFACSLPCPMMNLINGGMHADN
GLEFQEFMIRPIGASSIKEAVNMGADVFHTLKKLLHERGLSTGVGDEGGFAPNLASNEEALELLLLAIEKAGFTPGKDIS
LALDCAASSFYNVKTGTYDGRHYEEQIAILSNLCDRYPIDSIEDGLAEEDYDGWALLTEVLGEKVQIVGDDLFVTNPELI
LEGISNGLANSVLIKPNQIGTLTETVYAIKLAQMAGYTTIISHRSGETTDTTIADLAVAFNAGQIKTGSLSRSERVAKYN
RLMEIEEELGSEAIFTDSNVFSYEDSEE
>Mature_428_residues
MFEAVIADIQAREILDSRGYPTLHVKVTTSTGSVGEARVPSGASTGKKEALEFRDTDSPRYQGKGVLQAVKNVKEILFPL
VKGCSVYEQSLIDSLMMDSDGSPNKETLGANAILGVSLATAHAAAATLRRPLYRYLGGCFACSLPCPMMNLINGGMHADN
GLEFQEFMIRPIGASSIKEAVNMGADVFHTLKKLLHERGLSTGVGDEGGFAPNLASNEEALELLLLAIEKAGFTPGKDIS
LALDCAASSFYNVKTGTYDGRHYEEQIAILSNLCDRYPIDSIEDGLAEEDYDGWALLTEVLGEKVQIVGDDLFVTNPELI
LEGISNGLANSVLIKPNQIGTLTETVYAIKLAQMAGYTTIISHRSGETTDTTIADLAVAFNAGQIKTGSLSRSERVAKYN
RLMEIEEELGSEAIFTDSNVFSYEDSEE

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=432, Percent_Identity=49.7685185185185, Blast_Score=375, Evalue=1e-104,
Organism=Homo sapiens, GI301897477, Length=426, Percent_Identity=51.4084507042254, Blast_Score=371, Evalue=1e-103,
Organism=Homo sapiens, GI301897469, Length=426, Percent_Identity=51.4084507042254, Blast_Score=371, Evalue=1e-103,
Organism=Homo sapiens, GI4503571, Length=432, Percent_Identity=48.8425925925926, Blast_Score=370, Evalue=1e-102,
Organism=Homo sapiens, GI301897479, Length=424, Percent_Identity=47.1698113207547, Blast_Score=319, Evalue=3e-87,
Organism=Homo sapiens, GI169201331, Length=353, Percent_Identity=27.7620396600567, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI169201757, Length=353, Percent_Identity=27.7620396600567, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI239744207, Length=353, Percent_Identity=27.7620396600567, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI310129182, Length=139, Percent_Identity=33.8129496402878, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI310110045, Length=139, Percent_Identity=33.8129496402878, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI310120572, Length=139, Percent_Identity=33.8129496402878, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1789141, Length=422, Percent_Identity=55.9241706161137, Blast_Score=439, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI71995829, Length=433, Percent_Identity=49.6535796766744, Blast_Score=370, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17536383, Length=433, Percent_Identity=49.6535796766744, Blast_Score=370, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI32563855, Length=193, Percent_Identity=43.0051813471503, Blast_Score=154, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6321693, Length=441, Percent_Identity=47.6190476190476, Blast_Score=349, Evalue=4e-97,
Organism=Saccharomyces cerevisiae, GI6323985, Length=429, Percent_Identity=46.1538461538462, Blast_Score=327, Evalue=2e-90,
Organism=Saccharomyces cerevisiae, GI6324974, Length=429, Percent_Identity=46.1538461538462, Blast_Score=327, Evalue=2e-90,
Organism=Saccharomyces cerevisiae, GI6324969, Length=429, Percent_Identity=46.1538461538462, Blast_Score=327, Evalue=2e-90,
Organism=Saccharomyces cerevisiae, GI6321968, Length=441, Percent_Identity=46.031746031746, Blast_Score=320, Evalue=2e-88,
Organism=Drosophila melanogaster, GI24580918, Length=432, Percent_Identity=49.0740740740741, Blast_Score=346, Evalue=2e-95,
Organism=Drosophila melanogaster, GI24580916, Length=432, Percent_Identity=49.0740740740741, Blast_Score=346, Evalue=2e-95,
Organism=Drosophila melanogaster, GI24580920, Length=432, Percent_Identity=49.0740740740741, Blast_Score=346, Evalue=2e-95,
Organism=Drosophila melanogaster, GI24580914, Length=432, Percent_Identity=49.0740740740741, Blast_Score=346, Evalue=2e-95,
Organism=Drosophila melanogaster, GI281360527, Length=429, Percent_Identity=49.1841491841492, Blast_Score=344, Evalue=6e-95,
Organism=Drosophila melanogaster, GI17137654, Length=429, Percent_Identity=49.1841491841492, Blast_Score=344, Evalue=6e-95,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 46105; Mature: 46105

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEAVIADIQAREILDSRGYPTLHVKVTTSTGSVGEARVPSGASTGKKEALEFRDTDSPR
CCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHCCCCCCCC
YQGKGVLQAVKNVKEILFPLVKGCSVYEQSLIDSLMMDSDGSPNKETLGANAILGVSLAT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHH
AHAAAATLRRPLYRYLGGCFACSLPCPMMNLINGGMHADNGLEFQEFMIRPIGASSIKEA
HHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHCCCCCCCCCCCHHHHHHCCCCHHHHHHH
VNMGADVFHTLKKLLHERGLSTGVGDEGGFAPNLASNEEALELLLLAIEKAGFTPGKDIS
HHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCE
LALDCAASSFYNVKTGTYDGRHYEEQIAILSNLCDRYPIDSIEDGLAEEDYDGWALLTEV
EEEEECCCCCEECCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCHHHHHHHH
LGEKVQIVGDDLFVTNPELILEGISNGLANSVLIKPNQIGTLTETVYAIKLAQMAGYTTI
HCCCEEEECCCEEECCHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHCCHHHE
ISHRSGETTDTTIADLAVAFNAGQIKTGSLSRSERVAKYNRLMEIEEELGSEAIFTDSNV
EECCCCCCCHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCE
FSYEDSEE
ECCCCCCC
>Mature Secondary Structure
MFEAVIADIQAREILDSRGYPTLHVKVTTSTGSVGEARVPSGASTGKKEALEFRDTDSPR
CCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHCCCCCCCC
YQGKGVLQAVKNVKEILFPLVKGCSVYEQSLIDSLMMDSDGSPNKETLGANAILGVSLAT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHH
AHAAAATLRRPLYRYLGGCFACSLPCPMMNLINGGMHADNGLEFQEFMIRPIGASSIKEA
HHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHCCCCCCCCCCCHHHHHHCCCCHHHHHHH
VNMGADVFHTLKKLLHERGLSTGVGDEGGFAPNLASNEEALELLLLAIEKAGFTPGKDIS
HHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCE
LALDCAASSFYNVKTGTYDGRHYEEQIAILSNLCDRYPIDSIEDGLAEEDYDGWALLTEV
EEEEECCCCCEECCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCHHHHHHHH
LGEKVQIVGDDLFVTNPELILEGISNGLANSVLIKPNQIGTLTETVYAIKLAQMAGYTTI
HCCCEEEECCCEEECCHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHCCHHHE
ISHRSGETTDTTIADLAVAFNAGQIKTGSLSRSERVAKYNRLMEIEEELGSEAIFTDSNV
EECCCCCCCHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCE
FSYEDSEE
ECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]