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The map label for this gene is rpoN

Identifier: 15618680

GI number: 15618680

Start: 869141

End: 870463

Strand: Reverse

Name: rpoN

Synonym: CPn0771

Alternate gene names: 15618680

Gene position: 870463-869141 (Counterclockwise)

Preceding gene: 15618681

Following gene: 15618678

Centisome position: 70.76

GC content: 41.04

Gene sequence:

>1323_bases
ATGTTTCAGCAAAAGCAGAAGTTGTCTCTAAAGTATCTACCCTCACTAAGGATGCAACAAGGCCTGCAGATGCTGCAATC
GCCACTTACTGAGTTATCATCGTATGTAGTTCAAGAGATCATTGATAATCCTTTTTTTGATCTCTCTTCACTAGAAGAGG
AAGAATGGTCTCCTTGTTATCGACCTACAAACTCTACGTTTTCCTATTTGAATCAGACTCCTGGACCTCAAGAGTCTTTA
TATACTCGTCTCCTTCCTCAAATCGAGGAGGCTTTTTCTACTGCAGAAGAACGATTCATTGCCCATCAAATTGCCGGGAA
TCTCTCGGATGAAGGACTCTTTCTAAGAAATCCTGAGGATTTTGCTCAAGAACTTGAGCTTCCCTTAGAAAAAATTCATA
AAGTTTGGGACACTATACAAAACCTAAGTCCTGAAGGCATTGCCTCTCCTTCGCTACAAAGCTATTGGATGAAGCTCCTC
CGCAACTCCTCCCACCAACAAGCCTATAGCATCGTCCGTGATTGCTATCCCTTGATGACTAACTGTGAGTTTGCGCCTAT
TATGAAAAAGTTCAGTCTCTCTTTATCCGAACTTCGAAATATTTTAAAAAAAGCATTAGGATCTATACCTTGGTGTCCTG
CAGCAGCTTGCACTGTGAAGCCCATGGTATCAACTCCTCTTCCCGATATTTATCTTTTTTATTCCTCAGGATCTTGGAAA
ATTGAAGTGAGTACTCGAGGCTTGCCATCTATAAAGCTGAATAAAGAAACGTTTCACTTCTATGAACACCTTCCTAAAGA
AGAGCAGAAAAACCTCTCTCAACAAATTTTATCAGCAAAGTGGCTAATCAAAAATCTCAGAAAACGAGAACAAACGCTCC
TTCAAGTGATGGAGACACTTCTCCCCAAACAAGAAGACTTTTTATTAGGAAAAATTCCAGCCCCCTATCCTTTAGGCATT
AAAGATTTGGCTGAAGATCTCTCTTTTCATGAGTCAACAATCTTCCGTGCCATTGAAAACAAAGCGGTTGCAGCTCCTAT
AGGGATTTTCCCTCTAAAGCACCTCTTCCCCCGAGGAATCCATCAAGATTCCTCGCATTCTAAAGAGAATGTTCTACAAT
GGATCCGCCAATGGATCGCAACGGAACAAACTCCTCTATCTGATAGTGTGATCAGTGACAGAATCACTGCAAAAGGGATT
CCTTGTGCCCGACGTACAGTAGCAAAATACCGTGCACAACTAAAGATCCTGCCTGCTAATAAAAGAAAAAAGCTGTTTTA
CATAAGATCATCAAACAGTCATTTTAGAGATAGGCAGTTCTAG

Upstream 100 bases:

>100_bases
CCGCACAAGTTCGCAGCCTCTGGGGAACCGTACGGATGATGAAGCCCAGTAGATTTTTAAAGGAAATTCCAAAAGATTAT
ATGATTCAAGTGCGTTAGAC

Downstream 100 bases:

>100_bases
TTAAAGTTAAACAAAGTAGGATTGATAAATTTGCTTCCAACGGACATTTTCAAGCTTTCGTTTTCTAGCATAGTGTTCCA
AAACAGGAATAGGCTCTTTA

Product: RNA polymerase factor sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MFQQKQKLSLKYLPSLRMQQGLQMLQSPLTELSSYVVQEIIDNPFFDLSSLEEEEWSPCYRPTNSTFSYLNQTPGPQESL
YTRLLPQIEEAFSTAEERFIAHQIAGNLSDEGLFLRNPEDFAQELELPLEKIHKVWDTIQNLSPEGIASPSLQSYWMKLL
RNSSHQQAYSIVRDCYPLMTNCEFAPIMKKFSLSLSELRNILKKALGSIPWCPAAACTVKPMVSTPLPDIYLFYSSGSWK
IEVSTRGLPSIKLNKETFHFYEHLPKEEQKNLSQQILSAKWLIKNLRKREQTLLQVMETLLPKQEDFLLGKIPAPYPLGI
KDLAEDLSFHESTIFRAIENKAVAAPIGIFPLKHLFPRGIHQDSSHSKENVLQWIRQWIATEQTPLSDSVISDRITAKGI
PCARRTVAKYRAQLKILPANKRKKLFYIRSSNSHFRDRQF

Sequences:

>Translated_440_residues
MFQQKQKLSLKYLPSLRMQQGLQMLQSPLTELSSYVVQEIIDNPFFDLSSLEEEEWSPCYRPTNSTFSYLNQTPGPQESL
YTRLLPQIEEAFSTAEERFIAHQIAGNLSDEGLFLRNPEDFAQELELPLEKIHKVWDTIQNLSPEGIASPSLQSYWMKLL
RNSSHQQAYSIVRDCYPLMTNCEFAPIMKKFSLSLSELRNILKKALGSIPWCPAAACTVKPMVSTPLPDIYLFYSSGSWK
IEVSTRGLPSIKLNKETFHFYEHLPKEEQKNLSQQILSAKWLIKNLRKREQTLLQVMETLLPKQEDFLLGKIPAPYPLGI
KDLAEDLSFHESTIFRAIENKAVAAPIGIFPLKHLFPRGIHQDSSHSKENVLQWIRQWIATEQTPLSDSVISDRITAKGI
PCARRTVAKYRAQLKILPANKRKKLFYIRSSNSHFRDRQF
>Mature_440_residues
MFQQKQKLSLKYLPSLRMQQGLQMLQSPLTELSSYVVQEIIDNPFFDLSSLEEEEWSPCYRPTNSTFSYLNQTPGPQESL
YTRLLPQIEEAFSTAEERFIAHQIAGNLSDEGLFLRNPEDFAQELELPLEKIHKVWDTIQNLSPEGIASPSLQSYWMKLL
RNSSHQQAYSIVRDCYPLMTNCEFAPIMKKFSLSLSELRNILKKALGSIPWCPAAACTVKPMVSTPLPDIYLFYSSGSWK
IEVSTRGLPSIKLNKETFHFYEHLPKEEQKNLSQQILSAKWLIKNLRKREQTLLQVMETLLPKQEDFLLGKIPAPYPLGI
KDLAEDLSFHESTIFRAIENKAVAAPIGIFPLKHLFPRGIHQDSSHSKENVLQWIRQWIATEQTPLSDSVISDRITAKGI
PCARRTVAKYRAQLKILPANKRKKLFYIRSSNSHFRDRQF

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=469, Percent_Identity=27.5053304904051, Blast_Score=139, Evalue=4e-34,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 50733; Mature: 50733

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQQKQKLSLKYLPSLRMQQGLQMLQSPLTELSSYVVQEIIDNPFFDLSSLEEEEWSPCY
CCCCHHHCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHCCCCC
RPTNSTFSYLNQTPGPQESLYTRLLPQIEEAFSTAEERFIAHQIAGNLSDEGLFLRNPED
CCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCHHH
FAQELELPLEKIHKVWDTIQNLSPEGIASPSLQSYWMKLLRNSSHQQAYSIVRDCYPLMT
HHHHHHCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
NCEFAPIMKKFSLSLSELRNILKKALGSIPWCPAAACTVKPMVSTPLPDIYLFYSSGSWK
CCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCHHCCCCHHCCCCCCEEEEEECCCEE
IEVSTRGLPSIKLNKETFHFYEHLPKEEQKNLSQQILSAKWLIKNLRKREQTLLQVMETL
EEEECCCCCCEEECHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPKQEDFLLGKIPAPYPLGIKDLAEDLSFHESTIFRAIENKAVAAPIGIFPLKHLFPRGI
CCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCC
HQDSSHSKENVLQWIRQWIATEQTPLSDSVISDRITAKGIPCARRTVAKYRAQLKILPAN
CCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHEEEECCC
KRKKLFYIRSSNSHFRDRQF
CCCEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MFQQKQKLSLKYLPSLRMQQGLQMLQSPLTELSSYVVQEIIDNPFFDLSSLEEEEWSPCY
CCCCHHHCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHCCCCC
RPTNSTFSYLNQTPGPQESLYTRLLPQIEEAFSTAEERFIAHQIAGNLSDEGLFLRNPED
CCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCHHH
FAQELELPLEKIHKVWDTIQNLSPEGIASPSLQSYWMKLLRNSSHQQAYSIVRDCYPLMT
HHHHHHCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
NCEFAPIMKKFSLSLSELRNILKKALGSIPWCPAAACTVKPMVSTPLPDIYLFYSSGSWK
CCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCHHCCCCHHCCCCCCEEEEEECCCEE
IEVSTRGLPSIKLNKETFHFYEHLPKEEQKNLSQQILSAKWLIKNLRKREQTLLQVMETL
EEEECCCCCCEEECHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPKQEDFLLGKIPAPYPLGIKDLAEDLSFHESTIFRAIENKAVAAPIGIFPLKHLFPRGI
CCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCC
HQDSSHSKENVLQWIRQWIATEQTPLSDSVISDRITAKGIPCARRTVAKYRAQLKILPAN
CCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHEEEECCC
KRKKLFYIRSSNSHFRDRQF
CCCEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113171; 10984043 [H]