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The map label for this gene is rho

Identifier: 15618520

GI number: 15618520

Start: 700026

End: 701420

Strand: Reverse

Name: rho

Synonym: CPn0610

Alternate gene names: 15618520

Gene position: 701420-700026 (Counterclockwise)

Preceding gene: 15618521

Following gene: 15618518

Centisome position: 57.02

GC content: 42.15

Gene sequence:

>1395_bases
ATGAAAGAAGAGCGTTCTTCAGAAATCTTGCCTAGGGTGAAGGAAACGAAAAAACATGCTTACGTTTCTATGCAGGAAAA
ATCTTGTGTAGGGGAGTGTGCTGTAGTTGCCAGTGAGTCTGAAGAGGCCGAGTCTGTCACAGTTACAAAAATTGCTAAAC
TGCAAAGAATGGGAATTGAAGAGCTGAATATATTAGCTCGCCAATACGGTGTGAAAAATATAGGATCTCTAACAAAGTCT
CAGGTCGTCTTTGAGATTGTTAAAGCCAAGTCAGAGCGCCCAGATGAATTGCTGATAGGTGAAGGCGTGTTAGAAGTCCT
TCCAGACGGATTTGGATTTCTGAGATCCCCAACCTATAATTACCTACCTTCTGCTGAAGATATTTATGTTTCTCCAGCTC
AGATTCGTAGATTTGATCTCAAAAAAGGTGATACGATTATCGGTACGATACGCTCACCTAAAGAGAAAGAAAAGTACTTT
GCTCTATTGAAAGTAGATAAGATCAACGGATCTACTCCAGATAAAGCTAAAGAACGTGTACTTTTTGAAAACCTGACCCC
CCTCTACCCTAATCAAAGAATCGTGATGGAAATGGGGAAAGATCATTTGGCTGAGAGAGTCTTAGATCTTACCGCCCCCA
TTGGAAAAGGACAGCGGGGATTGATTGTAGCCCCACCAAGATCTGGGAAAACTGTCATTCTCCAAAGCATAGCACACGCG
ATTGCTGTGAATAATCCTGATATTGTTCTTATTGTCTTGTTAATTGACGAAAGACCCGAAGAAGTTACCGATATGATTCG
GCAAGTTCGTGGAGAAGTTGTTGCCTCGACATTTGACGAACAACCAGAAAGGCATATTCAGGTCGCTGAGATGGTTATAG
AGAAAGCTCGTCGTTTAGTGGAACATGGAAATGATGTGGTCATTCTACTCGATTCCATAACACGATTAGCACGTGCCTAT
AATACAGTTCAGCCGCATTCTGGAAAAATTCTTACCGGGGGTGTCGATGCTAGTGCATTGCACAAACCTAAGCGTTTCTT
CGGTGCTGCAAGAAATATTGAAGGCGGGGGATCGCTCACAATTCTTGCAACCGCTTTAATTGATACTGGTTCCAGAATGG
ACGAAGTGATTTTTGAAGAATTCAAAGGCACTGGAAATATGGAACTCGTCTTGGATCGACGTCTGTCAGATCGAAGAACC
TACCCCGCTATTGATTTAATTAAGAGTGGAACTAGAAAAGAAGAACTCCTCTATCATCCTAGTGAATTAGAAAGGGTCTA
CCTTTTCCGTCAGGCCATAGCAGATCTTACTACTATAGACGCCATGCATCTCTTGCTAGGAAGGTTGAAGAAAACCAATA
GTAACGCAGAATTCTTGTTGTCACTCAAAGAATAG

Upstream 100 bases:

>100_bases
AAAAGTTAGCACAAGCAGATGTTGTTGTTGAAAACAACGGAACTAAAAAAGAATTACATCAAAAAATTGAAGAATATTTT
TACGCTTTAAAGGGAGCATT

Downstream 100 bases:

>100_bases
TCTTGCTTAATCTTTGTTATGCTTTTAGGTAGGACGTCACTCAGTAGATTGTGTTGGCGTGCTTCCTATGAGTACTTCTG
GGAAAATAATTAAAGGTATC

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MKEERSSEILPRVKETKKHAYVSMQEKSCVGECAVVASESEEAESVTVTKIAKLQRMGIEELNILARQYGVKNIGSLTKS
QVVFEIVKAKSERPDELLIGEGVLEVLPDGFGFLRSPTYNYLPSAEDIYVSPAQIRRFDLKKGDTIIGTIRSPKEKEKYF
ALLKVDKINGSTPDKAKERVLFENLTPLYPNQRIVMEMGKDHLAERVLDLTAPIGKGQRGLIVAPPRSGKTVILQSIAHA
IAVNNPDIVLIVLLIDERPEEVTDMIRQVRGEVVASTFDEQPERHIQVAEMVIEKARRLVEHGNDVVILLDSITRLARAY
NTVQPHSGKILTGGVDASALHKPKRFFGAARNIEGGGSLTILATALIDTGSRMDEVIFEEFKGTGNMELVLDRRLSDRRT
YPAIDLIKSGTRKEELLYHPSELERVYLFRQAIADLTTIDAMHLLLGRLKKTNSNAEFLLSLKE

Sequences:

>Translated_464_residues
MKEERSSEILPRVKETKKHAYVSMQEKSCVGECAVVASESEEAESVTVTKIAKLQRMGIEELNILARQYGVKNIGSLTKS
QVVFEIVKAKSERPDELLIGEGVLEVLPDGFGFLRSPTYNYLPSAEDIYVSPAQIRRFDLKKGDTIIGTIRSPKEKEKYF
ALLKVDKINGSTPDKAKERVLFENLTPLYPNQRIVMEMGKDHLAERVLDLTAPIGKGQRGLIVAPPRSGKTVILQSIAHA
IAVNNPDIVLIVLLIDERPEEVTDMIRQVRGEVVASTFDEQPERHIQVAEMVIEKARRLVEHGNDVVILLDSITRLARAY
NTVQPHSGKILTGGVDASALHKPKRFFGAARNIEGGGSLTILATALIDTGSRMDEVIFEEFKGTGNMELVLDRRLSDRRT
YPAIDLIKSGTRKEELLYHPSELERVYLFRQAIADLTTIDAMHLLLGRLKKTNSNAEFLLSLKE
>Mature_464_residues
MKEERSSEILPRVKETKKHAYVSMQEKSCVGECAVVASESEEAESVTVTKIAKLQRMGIEELNILARQYGVKNIGSLTKS
QVVFEIVKAKSERPDELLIGEGVLEVLPDGFGFLRSPTYNYLPSAEDIYVSPAQIRRFDLKKGDTIIGTIRSPKEKEKYF
ALLKVDKINGSTPDKAKERVLFENLTPLYPNQRIVMEMGKDHLAERVLDLTAPIGKGQRGLIVAPPRSGKTVILQSIAHA
IAVNNPDIVLIVLLIDERPEEVTDMIRQVRGEVVASTFDEQPERHIQVAEMVIEKARRLVEHGNDVVILLDSITRLARAY
NTVQPHSGKILTGGVDASALHKPKRFFGAARNIEGGGSLTILATALIDTGSRMDEVIFEEFKGTGNMELVLDRRLSDRRT
YPAIDLIKSGTRKEELLYHPSELERVYLFRQAIADLTTIDAMHLLLGRLKKTNSNAEFLLSLKE

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=416, Percent_Identity=60.5769230769231, Blast_Score=516, Evalue=1e-147,
Organism=Escherichia coli, GI1788251, Length=285, Percent_Identity=29.1228070175439, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 51820; Mature: 51820

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEERSSEILPRVKETKKHAYVSMQEKSCVGECAVVASESEEAESVTVTKIAKLQRMGIE
CCCCCHHHHHHHHHHHHHHHEEEEHHHHCCCHHEEEECCCCCHHHHHHHHHHHHHHCCHH
ELNILARQYGVKNIGSLTKSQVVFEIVKAKSERPDELLIGEGVLEVLPDGFGFLRSPTYN
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHCCCCCHHHCCCCCC
YLPSAEDIYVSPAQIRRFDLKKGDTIIGTIRSPKEKEKYFALLKVDKINGSTPDKAKERV
CCCCCCCEEECHHHHHHEECCCCCEEEEECCCCHHHHHEEEEEEEEECCCCCCHHHHHHH
LFENLTPLYPNQRIVMEMGKDHLAERVLDLTAPIGKGQRGLIVAPPRSGKTVILQSIAHA
HHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHH
IAVNNPDIVLIVLLIDERPEEVTDMIRQVRGEVVASTFDEQPERHIQVAEMVIEKARRLV
HHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
EHGNDVVILLDSITRLARAYNTVQPHSGKILTGGVDASALHKPKRFFGAARNIEGGGSLT
HCCCCEEEEHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHCCHHHHHHHCCCCCCCCEE
ILATALIDTGSRMDEVIFEEFKGTGNMELVLDRRLSDRRTYPAIDLIKSGTRKEELLYHP
EEHHHHHHCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHCCCCHHHHEECH
SELERVYLFRQAIADLTTIDAMHLLLGRLKKTNSNAEFLLSLKE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECC
>Mature Secondary Structure
MKEERSSEILPRVKETKKHAYVSMQEKSCVGECAVVASESEEAESVTVTKIAKLQRMGIE
CCCCCHHHHHHHHHHHHHHHEEEEHHHHCCCHHEEEECCCCCHHHHHHHHHHHHHHCCHH
ELNILARQYGVKNIGSLTKSQVVFEIVKAKSERPDELLIGEGVLEVLPDGFGFLRSPTYN
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHCCCCCHHHCCCCCC
YLPSAEDIYVSPAQIRRFDLKKGDTIIGTIRSPKEKEKYFALLKVDKINGSTPDKAKERV
CCCCCCCEEECHHHHHHEECCCCCEEEEECCCCHHHHHEEEEEEEEECCCCCCHHHHHHH
LFENLTPLYPNQRIVMEMGKDHLAERVLDLTAPIGKGQRGLIVAPPRSGKTVILQSIAHA
HHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHH
IAVNNPDIVLIVLLIDERPEEVTDMIRQVRGEVVASTFDEQPERHIQVAEMVIEKARRLV
HHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
EHGNDVVILLDSITRLARAYNTVQPHSGKILTGGVDASALHKPKRFFGAARNIEGGGSLT
HCCCCEEEEHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHCCHHHHHHHCCCCCCCCEE
ILATALIDTGSRMDEVIFEEFKGTGNMELVLDRRLSDRRTYPAIDLIKSGTRKEELLYHP
EEHHHHHHCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHCCCCHHHHEECH
SELERVYLFRQAIADLTTIDAMHLLLGRLKKTNSNAEFLLSLKE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]