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The map label for this gene is oppA_5

Identifier: 15618509

GI number: 15618509

Start: 689679

End: 691802

Strand: Reverse

Name: oppA_5

Synonym: CPn0599

Alternate gene names: 15618509

Gene position: 691802-689679 (Counterclockwise)

Preceding gene: 15618510

Following gene: 15618508

Centisome position: 56.23

GC content: 41.67

Gene sequence:

>2124_bases
ATGTATAAAAGATGTGTGCTAGATAAAATTTTAAAGGGGATTGTCGCCGGTTCTTTAATTTTGTTATACTGGTCCTCAGA
CCTACTTGAAAGAGACATTAAGTCGATAAAAGGTAACGTAAGAGATATTCAAGAAGACATTCGTGAAATCTCACGCGTAG
TGAAACAACAGCAGACATCACAAGCTATCCCTGCGGCACCTGGGGTGATGCTCGCTCCTAAGCTCGTCAGAGACGAAGCT
TTTGCTCTACTCTTTGGAGATCCTAGTTATCCTAATTTACTTTCCCTAGACCCCTATAAACAGCAGACTCTTCCTGAACT
TCTAGGAACAAATTTCCACCCTCATGGTATCCTACGCACTGCCCATGTCGGAAAACCCGAAAATCTGAGCCCTTTTAATG
GCTTTGATTATGTCGTGGGCTTTTACGATCTCTGTATTCCTAGTTTAGCTTCTCCCCACGTAGGGAAATACGAAGAATTT
TCTCCAGATCTCGCTGTGAAAATAGAAGAACATCTTGTTGAAGATGGTTCTGGGGATAAAGAGTTTCACATCTATCTGAG
GCCGAATGTTTTTTGGCGTCCTATAGATCCTAAGGCCCTTCCAAAACACGTTCAGTTAGACGAAGTATTTCAACGTCCTC
ATCCTGTGACAGCTCATGATATTAAGTTTTTCTACGACGCTGTTATGAACCCTTATGTAGCAACCATGCGAGCAGTGGCT
CTGCGCTCTTGTTATGAAGATGTGGTTTCTGTCTCAGTAGAAAACGATTTAAAATTAGTAGTCAGATGGAAAGCACACAC
GGTAATCAATGAAGAAGGAAAGGAAGAGCGCAAAGTGCTCTACTCTGCATTTTCTAATACCTTAAGCTTGCAGCCCCTCC
CTAGATTTGTATATCAGTATTTTGCTAACGGGGAAAAAATCATTGAAGATGAGAATATCGATACCTACCGAACCAATTCC
ATTTGGGCGCAAAACTTCACTATGCATTGGGCAAACAACTATATTGTAAGTTGTGGAGCCTACTACTTTGCAGGGATGGA
TGATGAGAAAATCGTGTTTTCTAGAAATCCTGACTTCTATGATCCTCTTGCGGCTCTTATTGACAAGCGTTTCGTCTATT
TTAAGGAAAGCACAGACTCCCTATTCCAAGATTTTAAGACAGGGAAAATAGACATCTCTTACCTTCCACCCAACCAAAGA
GATAATTTCTATAGTTTTATGAAAAGCTCCGCTTATAACAAACAGGTAGCTAAGGGAGGAGCCGTCCGTGAAACAGTCTC
AGCAGATCGAGCATATACGTACATAGGATGGAATTGCTTTTCATTATTTTTCCAAAGCCGACAGGTGCGCTGTGCTATGA
ACATGGCAATCGATAGAGAGAGGATTATCGAACAGTGCTTGGATGGCCAAGGCTATACGATTAGTGGGCCTTTTGCTTCG
AGTTCTCCTTCTTATAATAAACAGATCGAAGGGTGGCATTATTCTCCAGAAGAAGCAGCTCGTCTCCTGGAAGAAGAGGG
ATGGATAGATACCGATGGCGATGGAATCCGAGAAAAAGTTATCGATGGTGTGATTGTCCCGTTCCGTTTCCGTTTATGCT
ATTATGTAAAGAGTGTCACCGCTCATACCATTGCAGATTACGTAGCTACTGCTTGTAAGGAAATCGGAATCGAGTGTAGC
CTTCTAGGACTAGATATGGCCGATCTTTCGCAAGCTTTTGATGAAAAGAATTTCGATGCTCTTTTAATGGGATGGTGTTT
AGGAATTCCTCCTGAGGATCCTAGGGCTTTATGGCATTCTGAAGGGGCTATGGAAAAGGGTTCAGCGAATGTTGTAGGTT
TCCATAATGAAGAAGCTGATAAAATCATAGACAGACTCAGCTACGAATACGATCTGAAAGAACGTAATCGCCTGTACCAC
CGTTTCCATGAAATTATTCATGAGGAAGCTCCTTATGCTTTCTTGTTCTCACGACATTGTTCCTTACTTTATAAGGATTA
TGTAAAAAATATTTTCGTACCTACACATAGAACAGATTTAATTCCTGAAGCTCAGGATGAGACTGTCAACGTAACTATGG
TATGGCTTGAGAAGAAGGAGGATCCGTGCTTAAGTACATCCTAA

Upstream 100 bases:

>100_bases
AGATTTTAATACTATGCAGCATCTAAGGTCCCTCGGATTAAATGGTTGGGGAGAGATCCCTATGAGTCCTTGCCTCTAAA
AGCGGAGGGAGAGTGGTCAC

Downstream 100 bases:

>100_bases
AACGTCTAGTTTTAATCCCCCTGACTTTATTTGCTATTGTCTCTATAAATTTTGTAATTTTAAATGCGGCTCCTGGAGAT
GTCTTGGAAGAAAAGTCTAG

Product: oligopeptide binding lipoprotein

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 707; Mature: 707

Protein sequence:

>707_residues
MYKRCVLDKILKGIVAGSLILLYWSSDLLERDIKSIKGNVRDIQEDIREISRVVKQQQTSQAIPAAPGVMLAPKLVRDEA
FALLFGDPSYPNLLSLDPYKQQTLPELLGTNFHPHGILRTAHVGKPENLSPFNGFDYVVGFYDLCIPSLASPHVGKYEEF
SPDLAVKIEEHLVEDGSGDKEFHIYLRPNVFWRPIDPKALPKHVQLDEVFQRPHPVTAHDIKFFYDAVMNPYVATMRAVA
LRSCYEDVVSVSVENDLKLVVRWKAHTVINEEGKEERKVLYSAFSNTLSLQPLPRFVYQYFANGEKIIEDENIDTYRTNS
IWAQNFTMHWANNYIVSCGAYYFAGMDDEKIVFSRNPDFYDPLAALIDKRFVYFKESTDSLFQDFKTGKIDISYLPPNQR
DNFYSFMKSSAYNKQVAKGGAVRETVSADRAYTYIGWNCFSLFFQSRQVRCAMNMAIDRERIIEQCLDGQGYTISGPFAS
SSPSYNKQIEGWHYSPEEAARLLEEEGWIDTDGDGIREKVIDGVIVPFRFRLCYYVKSVTAHTIADYVATACKEIGIECS
LLGLDMADLSQAFDEKNFDALLMGWCLGIPPEDPRALWHSEGAMEKGSANVVGFHNEEADKIIDRLSYEYDLKERNRLYH
RFHEIIHEEAPYAFLFSRHCSLLYKDYVKNIFVPTHRTDLIPEAQDETVNVTMVWLEKKEDPCLSTS

Sequences:

>Translated_707_residues
MYKRCVLDKILKGIVAGSLILLYWSSDLLERDIKSIKGNVRDIQEDIREISRVVKQQQTSQAIPAAPGVMLAPKLVRDEA
FALLFGDPSYPNLLSLDPYKQQTLPELLGTNFHPHGILRTAHVGKPENLSPFNGFDYVVGFYDLCIPSLASPHVGKYEEF
SPDLAVKIEEHLVEDGSGDKEFHIYLRPNVFWRPIDPKALPKHVQLDEVFQRPHPVTAHDIKFFYDAVMNPYVATMRAVA
LRSCYEDVVSVSVENDLKLVVRWKAHTVINEEGKEERKVLYSAFSNTLSLQPLPRFVYQYFANGEKIIEDENIDTYRTNS
IWAQNFTMHWANNYIVSCGAYYFAGMDDEKIVFSRNPDFYDPLAALIDKRFVYFKESTDSLFQDFKTGKIDISYLPPNQR
DNFYSFMKSSAYNKQVAKGGAVRETVSADRAYTYIGWNCFSLFFQSRQVRCAMNMAIDRERIIEQCLDGQGYTISGPFAS
SSPSYNKQIEGWHYSPEEAARLLEEEGWIDTDGDGIREKVIDGVIVPFRFRLCYYVKSVTAHTIADYVATACKEIGIECS
LLGLDMADLSQAFDEKNFDALLMGWCLGIPPEDPRALWHSEGAMEKGSANVVGFHNEEADKIIDRLSYEYDLKERNRLYH
RFHEIIHEEAPYAFLFSRHCSLLYKDYVKNIFVPTHRTDLIPEAQDETVNVTMVWLEKKEDPCLSTS
>Mature_707_residues
MYKRCVLDKILKGIVAGSLILLYWSSDLLERDIKSIKGNVRDIQEDIREISRVVKQQQTSQAIPAAPGVMLAPKLVRDEA
FALLFGDPSYPNLLSLDPYKQQTLPELLGTNFHPHGILRTAHVGKPENLSPFNGFDYVVGFYDLCIPSLASPHVGKYEEF
SPDLAVKIEEHLVEDGSGDKEFHIYLRPNVFWRPIDPKALPKHVQLDEVFQRPHPVTAHDIKFFYDAVMNPYVATMRAVA
LRSCYEDVVSVSVENDLKLVVRWKAHTVINEEGKEERKVLYSAFSNTLSLQPLPRFVYQYFANGEKIIEDENIDTYRTNS
IWAQNFTMHWANNYIVSCGAYYFAGMDDEKIVFSRNPDFYDPLAALIDKRFVYFKESTDSLFQDFKTGKIDISYLPPNQR
DNFYSFMKSSAYNKQVAKGGAVRETVSADRAYTYIGWNCFSLFFQSRQVRCAMNMAIDRERIIEQCLDGQGYTISGPFAS
SSPSYNKQIEGWHYSPEEAARLLEEEGWIDTDGDGIREKVIDGVIVPFRFRLCYYVKSVTAHTIADYVATACKEIGIECS
LLGLDMADLSQAFDEKNFDALLMGWCLGIPPEDPRALWHSEGAMEKGSANVVGFHNEEADKIIDRLSYEYDLKERNRLYH
RFHEIIHEEAPYAFLFSRHCSLLYKDYVKNIFVPTHRTDLIPEAQDETVNVTMVWLEKKEDPCLSTS

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789887, Length=314, Percent_Identity=25.796178343949, Blast_Score=79, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 81080; Mature: 81080

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKRCVLDKILKGIVAGSLILLYWSSDLLERDIKSIKGNVRDIQEDIREISRVVKQQQTS
CCHHHHHHHHHHHHHHHCEEEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
QAIPAAPGVMLAPKLVRDEAFALLFGDPSYPNLLSLDPYKQQTLPELLGTNFHPHGILRT
CCCCCCCCCHHCHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEE
AHVGKPENLSPFNGFDYVVGFYDLCIPSLASPHVGKYEEFSPDLAVKIEEHLVEDGSGDK
ECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCEEEEHHHHHCCCCCCC
EFHIYLRPNVFWRPIDPKALPKHVQLDEVFQRPHPVTAHDIKFFYDAVMNPYVATMRAVA
EEEEEECCCEEECCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHCHHHHHHHHHH
LRSCYEDVVSVSVENDLKLVVRWKAHTVINEEGKEERKVLYSAFSNTLSLQPLPRFVYQY
HHHHHHHHHEECCCCCEEEEEEEEHHEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHH
FANGEKIIEDENIDTYRTNSIWAQNFTMHWANNYIVSCGAYYFAGMDDEKIVFSRNPDFY
HCCCCEEEECCCCCEEECCCEEECCCEEEECCCEEEECCHHEECCCCCCEEEEECCCCCC
DPLAALIDKRFVYFKESTDSLFQDFKTGKIDISYLPPNQRDNFYSFMKSSAYNKQVAKGG
HHHHHHHHHHEEEEECCHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCC
AVRETVSADRAYTYIGWNCFSLFFQSRQVRCAMNMAIDRERIIEQCLDGQGYTISGPFAS
CHHHHHHCCCEEEEECHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHCCCCEEEECCCCC
SSPSYNKQIEGWHYSPEEAARLLEEEGWIDTDGDGIREKVIDGVIVPFRFRLCYYVKSVT
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHCCEEHHHHHHHHHHHHHHH
AHTIADYVATACKEIGIECSLLGLDMADLSQAFDEKNFDALLMGWCLGIPPEDPRALWHS
HHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHCC
EGAMEKGSANVVGFHNEEADKIIDRLSYEYDLKERNRLYHRFHEIIHEEAPYAFLFSRHC
CCCCCCCCCCEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
SLLYKDYVKNIFVPTHRTDLIPEAQDETVNVTMVWLEKKEDPCLSTS
HHHHHHHHHHEECCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCC
>Mature Secondary Structure
MYKRCVLDKILKGIVAGSLILLYWSSDLLERDIKSIKGNVRDIQEDIREISRVVKQQQTS
CCHHHHHHHHHHHHHHHCEEEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
QAIPAAPGVMLAPKLVRDEAFALLFGDPSYPNLLSLDPYKQQTLPELLGTNFHPHGILRT
CCCCCCCCCHHCHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEE
AHVGKPENLSPFNGFDYVVGFYDLCIPSLASPHVGKYEEFSPDLAVKIEEHLVEDGSGDK
ECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCEEEEHHHHHCCCCCCC
EFHIYLRPNVFWRPIDPKALPKHVQLDEVFQRPHPVTAHDIKFFYDAVMNPYVATMRAVA
EEEEEECCCEEECCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHCHHHHHHHHHH
LRSCYEDVVSVSVENDLKLVVRWKAHTVINEEGKEERKVLYSAFSNTLSLQPLPRFVYQY
HHHHHHHHHEECCCCCEEEEEEEEHHEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHH
FANGEKIIEDENIDTYRTNSIWAQNFTMHWANNYIVSCGAYYFAGMDDEKIVFSRNPDFY
HCCCCEEEECCCCCEEECCCEEECCCEEEECCCEEEECCHHEECCCCCCEEEEECCCCCC
DPLAALIDKRFVYFKESTDSLFQDFKTGKIDISYLPPNQRDNFYSFMKSSAYNKQVAKGG
HHHHHHHHHHEEEEECCHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCC
AVRETVSADRAYTYIGWNCFSLFFQSRQVRCAMNMAIDRERIIEQCLDGQGYTISGPFAS
CHHHHHHCCCEEEEECHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHCCCCEEEECCCCC
SSPSYNKQIEGWHYSPEEAARLLEEEGWIDTDGDGIREKVIDGVIVPFRFRLCYYVKSVT
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHCCEEHHHHHHHHHHHHHHH
AHTIADYVATACKEIGIECSLLGLDMADLSQAFDEKNFDALLMGWCLGIPPEDPRALWHS
HHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHCC
EGAMEKGSANVVGFHNEEADKIIDRLSYEYDLKERNRLYHRFHEIIHEEAPYAFLFSRHC
CCCCCCCCCCEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
SLLYKDYVKNIFVPTHRTDLIPEAQDETVNVTMVWLEKKEDPCLSTS
HHHHHHHHHHEECCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]