Click here to switch to the map view.

The map label for this gene is leuA2 [H]

Identifier: 15607049

GI number: 15607049

Start: 1469552

End: 1471126

Strand: Direct

Name: leuA2 [H]

Synonym: aq_2090

Alternate gene names: 15607049

Gene position: 1469552-1471126 (Clockwise)

Preceding gene: 15607048

Following gene: 15607050

Centisome position: 94.73

GC content: 48.0

Gene sequence:

>1575_bases
ATGGAGGCGAAAATGGGCGAAAAGGTCTACATATTCGACACCACTCTGAGAGACGGAGAACAGGCTCCCGGTTTTTCCAT
GACCACGGAAGAAAAGCTACAGATGGCTCATCAGCTCGCAAAACTCAACGTAGATATTATAGAGGCTGGATTTGCAGCAG
CGTCCAAGGGGGACTTTGAAGCCGTAAACAGGATAGCAAAAGAAGTCAAAGGTCCTGTTATATGCTCCCTTGCAAGGGCA
CTCGAAAGTGACATAGAAATTGCCGCAAAGGCACTCGAACCTGCGGAGAGAAAAAGGATACACACCTTCATAGCCACCTC
TCCCATACATATGGAGTACAAACTCCGTATGACGCCGGACCAAGTTTTAGAGAGAATTAAAAAAGCTGTTTCCTTTGCGA
GGAACTTCACCGACGACGTTGAGTTTTCCTGCGAAGACGCCACGAGAAGCGAGAGGGAATTTCTCTACAGGGCCATAGAA
ACAGCCATCAAGCACGGGGCAACCGTTATAAATATCCCAGACACAGTGGGATACGCCATACCCGAGGAGTTCGGTCAATT
AATAGAGGACATAATGAACAACGTCCCGAATATAGATAAGGTTATTCTGAGCGTTCACTGTCACGACGACCTCGGACTTG
CCACTGCAAACTCCCTGACCGCGGTAAAACACGGGGCCAGACAGGTTGAATGTACCATAAATGGGATAGGGGAAAGAGCT
GGAAACGCAGCTCTTGAAGAGGTGGTAATGGCACTAAAAGTGAGAAAGGACTTTTTCGGAGACCTTTACACCGATGTGAA
TACGAAAGAGATTTACAAAACTTCCAGACTGCTTTGTAGGATTACAGGAAACTTCGTTCAGCCTAACAAGGCAATCGTCG
GGGATAACGCCTTTGCCCACGAGAGCGGAATACACCAGCACGGAGTACTCAGCCACAGAATGACCTACGAGATAATGAAC
CCCGAAGACGTTGGATTCCCCATGAGCAGAATAGTTCTCGGAAAGCACAGCGGAAGACACGCCCTGAAGAGAAGACTTGA
AGAACTCGGCTTTAAGTTCACCAAAGAAGAACTCGACAGGATATTCGAAAAGTTCAAGGAGCTTGCCGACAGAAAGAAGG
AAGTTTACGACGAAGACCTCGAAGCACTAATATACCAGGAGTTCATGAAGATAGAGGACCACGAACCCGTAAAGGTTCTC
CACTTTCAAGTTCAAAGCGGTGATAACATGATACCGACAGCAACGGTAAAGCTCGAGTTTAAAGGAGAAGAGAGGGAAGC
GAGTTCCACAGGAAACGGACCCGTTGACGCGACCATTAAAGCAATTCAGAAAGCCCTCGGCATAGAACCTAAACTCCTGG
ACTACTCAATTAAAGCCCTCACTCCCAACACGGACGCTCAGGCAGAGGCAAGGGTAGTCTTGGAGCTTGACGGGGTGAAA
GCCTCCGGAAGGGGTGTGGATACAGACATAATAAAGGCGAGTGTTAAGGCGTTTACCGACGCCCTGAATAGGGCTATAGT
CAGGAAAGAGTACATAATCCAGAGACAGGAAATCAGAGAAGAAGGTACCGTTTGA

Upstream 100 bases:

>100_bases
ATCCCTACAAAAAGTACTTACAGAAAAGGGTTGAACAGAAGGCTTAAATTTTTCCTCTTTTCTGTTGACTTTTCTTATTT
GAGAAATATTTTCAATAACC

Downstream 100 bases:

>100_bases
TGTGGAGAATTTTCTTCACAAGGTTCTTCGTAAGGTTTATCCTTTTCCCCCTCCTTGCTGTAATTATGGGAGTTCTTGTT
CTGGGAACGATATTCTTCAT

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 524; Mature: 524

Protein sequence:

>524_residues
MEAKMGEKVYIFDTTLRDGEQAPGFSMTTEEKLQMAHQLAKLNVDIIEAGFAAASKGDFEAVNRIAKEVKGPVICSLARA
LESDIEIAAKALEPAERKRIHTFIATSPIHMEYKLRMTPDQVLERIKKAVSFARNFTDDVEFSCEDATRSEREFLYRAIE
TAIKHGATVINIPDTVGYAIPEEFGQLIEDIMNNVPNIDKVILSVHCHDDLGLATANSLTAVKHGARQVECTINGIGERA
GNAALEEVVMALKVRKDFFGDLYTDVNTKEIYKTSRLLCRITGNFVQPNKAIVGDNAFAHESGIHQHGVLSHRMTYEIMN
PEDVGFPMSRIVLGKHSGRHALKRRLEELGFKFTKEELDRIFEKFKELADRKKEVYDEDLEALIYQEFMKIEDHEPVKVL
HFQVQSGDNMIPTATVKLEFKGEEREASSTGNGPVDATIKAIQKALGIEPKLLDYSIKALTPNTDAQAEARVVLELDGVK
ASGRGVDTDIIKASVKAFTDALNRAIVRKEYIIQRQEIREEGTV

Sequences:

>Translated_524_residues
MEAKMGEKVYIFDTTLRDGEQAPGFSMTTEEKLQMAHQLAKLNVDIIEAGFAAASKGDFEAVNRIAKEVKGPVICSLARA
LESDIEIAAKALEPAERKRIHTFIATSPIHMEYKLRMTPDQVLERIKKAVSFARNFTDDVEFSCEDATRSEREFLYRAIE
TAIKHGATVINIPDTVGYAIPEEFGQLIEDIMNNVPNIDKVILSVHCHDDLGLATANSLTAVKHGARQVECTINGIGERA
GNAALEEVVMALKVRKDFFGDLYTDVNTKEIYKTSRLLCRITGNFVQPNKAIVGDNAFAHESGIHQHGVLSHRMTYEIMN
PEDVGFPMSRIVLGKHSGRHALKRRLEELGFKFTKEELDRIFEKFKELADRKKEVYDEDLEALIYQEFMKIEDHEPVKVL
HFQVQSGDNMIPTATVKLEFKGEEREASSTGNGPVDATIKAIQKALGIEPKLLDYSIKALTPNTDAQAEARVVLELDGVK
ASGRGVDTDIIKASVKAFTDALNRAIVRKEYIIQRQEIREEGTV
>Mature_524_residues
MEAKMGEKVYIFDTTLRDGEQAPGFSMTTEEKLQMAHQLAKLNVDIIEAGFAAASKGDFEAVNRIAKEVKGPVICSLARA
LESDIEIAAKALEPAERKRIHTFIATSPIHMEYKLRMTPDQVLERIKKAVSFARNFTDDVEFSCEDATRSEREFLYRAIE
TAIKHGATVINIPDTVGYAIPEEFGQLIEDIMNNVPNIDKVILSVHCHDDLGLATANSLTAVKHGARQVECTINGIGERA
GNAALEEVVMALKVRKDFFGDLYTDVNTKEIYKTSRLLCRITGNFVQPNKAIVGDNAFAHESGIHQHGVLSHRMTYEIMN
PEDVGFPMSRIVLGKHSGRHALKRRLEELGFKFTKEELDRIFEKFKELADRKKEVYDEDLEALIYQEFMKIEDHEPVKVL
HFQVQSGDNMIPTATVKLEFKGEEREASSTGNGPVDATIKAIQKALGIEPKLLDYSIKALTPNTDAQAEARVVLELDGVK
ASGRGVDTDIIKASVKAFTDALNRAIVRKEYIIQRQEIREEGTV

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=501, Percent_Identity=50.499001996008, Blast_Score=488, Evalue=1e-139,
Organism=Saccharomyces cerevisiae, GI6320019, Length=378, Percent_Identity=28.042328042328, Blast_Score=131, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6320071, Length=378, Percent_Identity=28.042328042328, Blast_Score=130, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6324225, Length=537, Percent_Identity=24.0223463687151, Blast_Score=127, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6324682, Length=559, Percent_Identity=23.2558139534884, Blast_Score=123, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24582381, Length=293, Percent_Identity=25.2559726962457, Blast_Score=77, Evalue=4e-14,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 58564; Mature: 58564

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAKMGEKVYIFDTTLRDGEQAPGFSMTTEEKLQMAHQLAKLNVDIIEAGFAAASKGDFE
CCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHEECCCHHCCCCCHH
AVNRIAKEVKGPVICSLARALESDIEIAAKALEPAERKRIHTFIATSPIHMEYKLRMTPD
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEECCCEEEEEEEECCHH
QVLERIKKAVSFARNFTDDVEFSCEDATRSEREFLYRAIETAIKHGATVINIPDTVGYAI
HHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCHHCCCC
PEEFGQLIEDIMNNVPNIDKVILSVHCHDDLGLATANSLTAVKHGARQVECTINGIGERA
HHHHHHHHHHHHHCCCCHHHEEEEEEECCCCCCCCCCHHHHHHCCCEEEEEEEECCCCCC
GNAALEEVVMALKVRKDFFGDLYTDVNTKEIYKTSRLLCRITGNFVQPNKAIVGDNAFAH
CHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEECCCCCCCCEEECCCCHHH
ESGIHQHGVLSHRMTYEIMNPEDVGFPMSRIVLGKHSGRHALKRRLEELGFKFTKEELDR
CCCCCCCCCHHHCEEEEECCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHH
IFEKFKELADRKKEVYDEDLEALIYQEFMKIEDHEPVKVLHFQVQSGDNMIPTATVKLEF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCEEEEEEEE
KGEEREASSTGNGPVDATIKAIQKALGIEPKLLDYSIKALTPNTDAQAEARVVLELDGVK
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHCCEEEEEECCCCCCCCCEEEEEEEECCC
ASGRGVDTDIIKASVKAFTDALNRAIVRKEYIIQRQEIREEGTV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEAKMGEKVYIFDTTLRDGEQAPGFSMTTEEKLQMAHQLAKLNVDIIEAGFAAASKGDFE
CCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHEECCCHHCCCCCHH
AVNRIAKEVKGPVICSLARALESDIEIAAKALEPAERKRIHTFIATSPIHMEYKLRMTPD
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEECCCEEEEEEEECCHH
QVLERIKKAVSFARNFTDDVEFSCEDATRSEREFLYRAIETAIKHGATVINIPDTVGYAI
HHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCHHCCCC
PEEFGQLIEDIMNNVPNIDKVILSVHCHDDLGLATANSLTAVKHGARQVECTINGIGERA
HHHHHHHHHHHHHCCCCHHHEEEEEEECCCCCCCCCCHHHHHHCCCEEEEEEEECCCCCC
GNAALEEVVMALKVRKDFFGDLYTDVNTKEIYKTSRLLCRITGNFVQPNKAIVGDNAFAH
CHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEECCCCCCCCEEECCCCHHH
ESGIHQHGVLSHRMTYEIMNPEDVGFPMSRIVLGKHSGRHALKRRLEELGFKFTKEELDR
CCCCCCCCCHHHCEEEEECCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHH
IFEKFKELADRKKEVYDEDLEALIYQEFMKIEDHEPVKVLHFQVQSGDNMIPTATVKLEF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCEEEEEEEE
KGEEREASSTGNGPVDATIKAIQKALGIEPKLLDYSIKALTPNTDAQAEARVVLELDGVK
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHCCEEEEEECCCCCCCCCEEEEEEEECCC
ASGRGVDTDIIKASVKAFTDALNRAIVRKEYIIQRQEIREEGTV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]