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The map label for this gene is masA

Identifier: 15606973

GI number: 15606973

Start: 1391225

End: 1391896

Strand: Direct

Name: masA

Synonym: aq_1977

Alternate gene names: 15606973

Gene position: 1391225-1391896 (Clockwise)

Preceding gene: 15606972

Following gene: 15606974

Centisome position: 89.68

GC content: 42.86

Gene sequence:

>672_bases
ATGGTTAAGGCTATTCTTCTTGACATAGAGGGCACTATCGCACCCCTTTCCTTTGTAAAGGAAGTAATGTTTCCCTACTC
AAAGAAGAAGTTAAGGGAATTCCTTGAAAAAAACTGGGAAAAACCTGAGATAAAGAAGATTGTGCAGGAAGTGGAAAAGA
TAGAGGGAAGGGAACTCTCCCTTGAGGAAGCAGTGCAGCTTTTCTCAAGATGGATAGACGAGGACAGGAAAATAACACCG
CTTAAAGAACTTCAGGGACACATATGGGAAGAAGGATTCAAAAGCGGTGAGCTGAAAGCCCCCCTGTACGAAGACGCCTA
CGAGAAAATAAAGGAGTGGAAAGAGAAAGGTATTCCCGTTTACATATACTCTTCTGGTTCTGTAAAGGCTCAGAATCTTT
TTTTCGGTCACAGCGTTTACGGGGACATAAGGAACTTATTTTCAGGTTTCTTTGATACGAAGATAGGAAGCAAAAGGGAA
AGAAGTTCTTACGAGAAGATAGCCAAAGAAATAGGTCTTCCTCCTCACGAGATACTCTTTATCTCGGACAATCCGGAAGA
ACTCAAAGCTGCAAAAGAAGCGGGAATGAAGGTAATTCAGTCCGTAAGGGAAGGTGTTGAGCCGTCAGGGGACTTTGAGA
AGATAACTTCTTTCAGGGAGCTGGAGATATGA

Upstream 100 bases:

>100_bases
TTCAAGTGTATAAGGCTCTTCACGACACCCGAAGGCTGGGTAGCCGAATATACCGGCTCGGATATAGCGGAAAAGTTCCC
CAAGTACGAGGAAGTTGTAA

Downstream 100 bases:

>100_bases
TTTTATTCTCTCCTAAGGAGATAAAAGATATTGAAGAGCTTGTAAAGGAAAGGGAAGGAAGTGTAGAGGAAATAGAGGTA
ATCAAACTGAACGAGACTCC

Product: enolase-phosphatase E-1

Products: NA

Alternate protein names: 2,3-diketo-5-methylthio-1-phosphopentane phosphatase

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MVKAILLDIEGTIAPLSFVKEVMFPYSKKKLREFLEKNWEKPEIKKIVQEVEKIEGRELSLEEAVQLFSRWIDEDRKITP
LKELQGHIWEEGFKSGELKAPLYEDAYEKIKEWKEKGIPVYIYSSGSVKAQNLFFGHSVYGDIRNLFSGFFDTKIGSKRE
RSSYEKIAKEIGLPPHEILFISDNPEELKAAKEAGMKVIQSVREGVEPSGDFEKITSFRELEI

Sequences:

>Translated_223_residues
MVKAILLDIEGTIAPLSFVKEVMFPYSKKKLREFLEKNWEKPEIKKIVQEVEKIEGRELSLEEAVQLFSRWIDEDRKITP
LKELQGHIWEEGFKSGELKAPLYEDAYEKIKEWKEKGIPVYIYSSGSVKAQNLFFGHSVYGDIRNLFSGFFDTKIGSKRE
RSSYEKIAKEIGLPPHEILFISDNPEELKAAKEAGMKVIQSVREGVEPSGDFEKITSFRELEI
>Mature_223_residues
MVKAILLDIEGTIAPLSFVKEVMFPYSKKKLREFLEKNWEKPEIKKIVQEVEKIEGRELSLEEAVQLFSRWIDEDRKITP
LKELQGHIWEEGFKSGELKAPLYEDAYEKIKEWKEKGIPVYIYSSGSVKAQNLFFGHSVYGDIRNLFSGFFDTKIGSKRE
RSSYEKIAKEIGLPPHEILFISDNPEELKAAKEAGMKVIQSVREGVEPSGDFEKITSFRELEI

Specific function: Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone

COG id: COG4229

COG function: function code C; Predicted enolase-phosphatase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. MasA/mtnC family

Homologues:

Organism=Homo sapiens, GI10864017, Length=246, Percent_Identity=42.6829268292683, Blast_Score=191, Evalue=6e-49,
Organism=Caenorhabditis elegans, GI115533232, Length=218, Percent_Identity=41.2844036697248, Blast_Score=168, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6320797, Length=227, Percent_Identity=37.8854625550661, Blast_Score=146, Evalue=3e-36,
Organism=Drosophila melanogaster, GI281360080, Length=239, Percent_Identity=39.7489539748954, Blast_Score=186, Evalue=8e-48,
Organism=Drosophila melanogaster, GI24644163, Length=239, Percent_Identity=39.7489539748954, Blast_Score=186, Evalue=8e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MTNC_AQUAE (O67786)

Other databases:

- EMBL:   AE000657
- PIR:   F70469
- RefSeq:   NP_214355.1
- HSSP:   Q9UHY7
- ProteinModelPortal:   O67786
- SMR:   O67786
- GeneID:   1193683
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_1977
- NMPDR:   fig|224324.1.peg.1370
- HOGENOM:   HBG294242
- OMA:   TTDLNFI
- PhylomeDB:   O67786
- ProtClustDB:   CLSK866737
- BioCyc:   AAEO224324:AQ_1977-MONOMER
- HAMAP:   MF_01681
- InterPro:   IPR005834
- InterPro:   IPR010041
- InterPro:   IPR023214
- InterPro:   IPR006439
- Gene3D:   G3DSA:3.40.50.1000
- PANTHER:   PTHR20371
- TIGRFAMs:   TIGR01691
- TIGRFAMs:   TIGR01549

Pfam domain/function: PF00702 Hydrolase; SSF56784 SSF56784

EC number: =3.1.3.77

Molecular weight: Translated: 25794; Mature: 25794

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKAILLDIEGTIAPLSFVKEVMFPYSKKKLREFLEKNWEKPEIKKIVQEVEKIEGRELS
CCEEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCC
LEEAVQLFSRWIDEDRKITPLKELQGHIWEEGFKSGELKAPLYEDAYEKIKEWKEKGIPV
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCE
YIYSSGSVKAQNLFFGHSVYGDIRNLFSGFFDTKIGSKRERSSYEKIAKEIGLPPHEILF
EEECCCCEEHHHEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEE
ISDNPEELKAAKEAGMKVIQSVREGVEPSGDFEKITSFRELEI
ECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MVKAILLDIEGTIAPLSFVKEVMFPYSKKKLREFLEKNWEKPEIKKIVQEVEKIEGRELS
CCEEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCC
LEEAVQLFSRWIDEDRKITPLKELQGHIWEEGFKSGELKAPLYEDAYEKIKEWKEKGIPV
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCE
YIYSSGSVKAQNLFFGHSVYGDIRNLFSGFFDTKIGSKRERSSYEKIAKEIGLPPHEILF
EEECCCCEEHHHEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEE
ISDNPEELKAAKEAGMKVIQSVREGVEPSGDFEKITSFRELEI
ECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320