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The map label for this gene is purU [H]

Identifier: 15606867

GI number: 15606867

Start: 1280345

End: 1281196

Strand: Direct

Name: purU [H]

Synonym: aq_1818

Alternate gene names: 15606867

Gene position: 1280345-1281196 (Clockwise)

Preceding gene: 15606865

Following gene: 15606868

Centisome position: 82.53

GC content: 43.78

Gene sequence:

>852_bases
ATGCAAAACGCCATACTTCTCGTATCCTGCCCAGATAGGAAGGGACTCGTAAAGGAAATTTCCTCCTTTATAGCGGACAA
CGGGGGAAACATAGTCAGCTTTGACCAGCACATAGACGAGCAGACGAAAACCTTCCTTGCAAGGGTGGAGTGGAGTCTCG
AGGATTTTAAAATCCCAAGAGAAAAAATTGAAAATGAGTTTAAAAAAGTTGCCCAAAAGTTTTCTATGAATTTCCAAATT
AGTTTTTCCGATTACGTTAAAAAGGTTGCCATTTTCGTTTCAAAACAGGAGCACTGCTTTTACGACCTAATGCACAGATT
TTACTCGGGAGAGCTAAAGGGGGAGGTGAAGCTCGTTATAAGTAACCACGAAAAGGCGAGGAAAACAGCGGAGTTCTTCG
GCGTTCCCTTTTACCACATTCCGAAAACCAAGGAGAATAAACTTGAGGCTGAAAAGAGAGAGCTTGAGCTTTTAAAGGAG
TACGGTGTTGAACTTGTAGTTCTCGCAAGGTACATGCAAATTCTTTCCCCTAAATTCGTAAAGGAGTATGAAAACAAGAT
AATAAACATACACCATTCCTTCCTGCCAGCCTTTCCAGGGGCAAAACCCTACGAGAGAGCTTTCGGGAAAGGCGTGAAGA
TTATAGGTGCTACCGCCCATTACGTTACGGAGGAGCTGGACGAAGGCCCCATAATAGAGCAGGACGTGGTTCGTGTAAGC
CACAAGGACAGTCTTGAGGACTTCATAAGAAAGGGGAAGGACATAGAAAAAGTAGTTCTCGCAAGGGCGGTAAAGTGGCA
CCTTGAGGACAAGATACTCGTCTACAATGGAAAGACAGTAGTTTTTGAATGA

Upstream 100 bases:

>100_bases
ATTGAGTTCTTCGGCTATCTTTTTCAAAACGCCTTCGGAAACTAAAAGGTGTTGCCCGAAGGATTTTTTCAGTCTTACCA
TTAACTATTAAAATAACTCT

Downstream 100 bases:

>100_bases
AAATTTAATTATGAAGATAATTCTACTTTCACTCTTCTGGGTATTCTTTTCCTTCGGAAAGATATTCGTAGCCCAGTGGG
AAGGCCCCATAACGCCCACT

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MQNAILLVSCPDRKGLVKEISSFIADNGGNIVSFDQHIDEQTKTFLARVEWSLEDFKIPREKIENEFKKVAQKFSMNFQI
SFSDYVKKVAIFVSKQEHCFYDLMHRFYSGELKGEVKLVISNHEKARKTAEFFGVPFYHIPKTKENKLEAEKRELELLKE
YGVELVVLARYMQILSPKFVKEYENKIINIHHSFLPAFPGAKPYERAFGKGVKIIGATAHYVTEELDEGPIIEQDVVRVS
HKDSLEDFIRKGKDIEKVVLARAVKWHLEDKILVYNGKTVVFE

Sequences:

>Translated_283_residues
MQNAILLVSCPDRKGLVKEISSFIADNGGNIVSFDQHIDEQTKTFLARVEWSLEDFKIPREKIENEFKKVAQKFSMNFQI
SFSDYVKKVAIFVSKQEHCFYDLMHRFYSGELKGEVKLVISNHEKARKTAEFFGVPFYHIPKTKENKLEAEKRELELLKE
YGVELVVLARYMQILSPKFVKEYENKIINIHHSFLPAFPGAKPYERAFGKGVKIIGATAHYVTEELDEGPIIEQDVVRVS
HKDSLEDFIRKGKDIEKVVLARAVKWHLEDKILVYNGKTVVFE
>Mature_283_residues
MQNAILLVSCPDRKGLVKEISSFIADNGGNIVSFDQHIDEQTKTFLARVEWSLEDFKIPREKIENEFKKVAQKFSMNFQI
SFSDYVKKVAIFVSKQEHCFYDLMHRFYSGELKGEVKLVISNHEKARKTAEFFGVPFYHIPKTKENKLEAEKRELELLKE
YGVELVVLARYMQILSPKFVKEYENKIINIHHSFLPAFPGAKPYERAFGKGVKIIGATAHYVTEELDEGPIIEQDVVRVS
HKDSLEDFIRKGKDIEKVVLARAVKWHLEDKILVYNGKTVVFE

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=241, Percent_Identity=29.4605809128631, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI209869995, Length=241, Percent_Identity=29.4605809128631, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI209869993, Length=241, Percent_Identity=29.4605809128631, Blast_Score=99, Evalue=3e-21,
Organism=Escherichia coli, GI1787483, Length=286, Percent_Identity=39.1608391608392, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI1788846, Length=192, Percent_Identity=29.6875, Blast_Score=94, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17567511, Length=166, Percent_Identity=38.5542168674699, Blast_Score=117, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24582400, Length=234, Percent_Identity=29.0598290598291, Blast_Score=78, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 32819; Mature: 32819

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNAILLVSCPDRKGLVKEISSFIADNGGNIVSFDQHIDEQTKTFLARVEWSLEDFKIPR
CCCCEEEEECCCCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHCCHHHHCCCH
EKIENEFKKVAQKFSMNFQISFSDYVKKVAIFVSKQEHCFYDLMHRFYSGELKGEVKLVI
HHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEEE
SNHEKARKTAEFFGVPFYHIPKTKENKLEAEKRELELLKEYGVELVVLARYMQILSPKFV
ECCHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHH
KEYENKIINIHHSFLPAFPGAKPYERAFGKGVKIIGATAHYVTEELDEGPIIEQDVVRVS
HHHHHHHEEEHHHHCCCCCCCCHHHHHHCCCEEEEECHHHHHHHHCCCCCCCHHHHHHHH
HKDSLEDFIRKGKDIEKVVLARAVKWHLEDKILVYNGKTVVFE
CCCHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEEECCEEEEC
>Mature Secondary Structure
MQNAILLVSCPDRKGLVKEISSFIADNGGNIVSFDQHIDEQTKTFLARVEWSLEDFKIPR
CCCCEEEEECCCCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHCCHHHHCCCH
EKIENEFKKVAQKFSMNFQISFSDYVKKVAIFVSKQEHCFYDLMHRFYSGELKGEVKLVI
HHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEEE
SNHEKARKTAEFFGVPFYHIPKTKENKLEAEKRELELLKEYGVELVVLARYMQILSPKFV
ECCHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHH
KEYENKIINIHHSFLPAFPGAKPYERAFGKGVKIIGATAHYVTEELDEGPIIEQDVVRVS
HHHHHHHEEEHHHHCCCCCCCCHHHHHHCCCEEEEECHHHHHHHHCCCCCCCHHHHHHHH
HKDSLEDFIRKGKDIEKVVLARAVKWHLEDKILVYNGKTVVFE
CCCHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]