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The map label for this gene is shyS

Identifier: 15606714

GI number: 15606714

Start: 1121063

End: 1121713

Strand: Direct

Name: shyS

Synonym: aq_1591

Alternate gene names: NA

Gene position: 1121063-1121713 (Clockwise)

Preceding gene: 15606713

Following gene: 15606715

Centisome position: 72.26

GC content: 47.47

Gene sequence:

>651_bases
ATGAAGCTTTTTAACTTCAAAGGGGAAAGGGTATTCACGCCAGGTCCAGTAGAGATACCCGACAGAATAAGGGAAGTTTT
AGGCAGACAGATAATACACCACAGAACTAAGGAGTTTACGGAAGCCTTTTTAGAAACGAGGGAGCTCTTTAAAAGACTCG
TTGATTGCACTTCCGAAAACTTTGTGTTTTTTGCATCCTCGGGCACGGGGGCTATGGAAGCTTCCGTTCAGAACTTCTTT
AATCCCGGAGAGAAAGTTCTTGCCGTAGTAGGCGGAAAGTTCGGAGAAAGGTGGGCAGAGCTCGGAAGAACCTGGGGCCT
TGAGGTAGTAGAACTTCAGGTAGAGTGGGGAAAGAGCGTAGACCCCGATCAGGTGGAAGATATCCTGAATAAAAATCCCG
AGATAAAAGGTGTTCTGGTTCAGATGTCCGAAAGTTCCACGGGAGCCTACCACGACGTAAAAACCCTTGCCAAGATAACA
AAAGACAGGGATACACTCCTCGTAGTTGATGCGATAACGGCTCTCGGTGTGTACAATATGAAGCCCCACGAATGGGGACT
TGACGTAGTGGTGGGAGGTTCTCAAAAAGCTTTCATGCTTCCACCCGGGCTTTCAATGCTCTGTTCTCCGAAAAGGCGGA
AAAAAGGCTAA

Upstream 100 bases:

>100_bases
AGTTCGGCACGGACAAGGGTAAAAACGCCATACACGCCTCAGACTCACCCGAGAGTGCACAGTACGAGATCTGTTTCATA
TTCTCCGGTCTTGAGATAGT

Downstream 100 bases:

>100_bases
ACGACAGGGCTTATTACTTTTCCGTAAAGAAAGAAGTTGAAAAGCAAAGGGAGGGACAAACCGCTTACACTCCCGCAATA
ACCCTTATCCTTGCCCTGAG

Product: soluble hydrogenase small subunit

Products: NA

Alternate protein names: Tritium exchange subunit [H]

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MKLFNFKGERVFTPGPVEIPDRIREVLGRQIIHHRTKEFTEAFLETRELFKRLVDCTSENFVFFASSGTGAMEASVQNFF
NPGEKVLAVVGGKFGERWAELGRTWGLEVVELQVEWGKSVDPDQVEDILNKNPEIKGVLVQMSESSTGAYHDVKTLAKIT
KDRDTLLVVDAITALGVYNMKPHEWGLDVVVGGSQKAFMLPPGLSMLCSPKRRKKG

Sequences:

>Translated_216_residues
MKLFNFKGERVFTPGPVEIPDRIREVLGRQIIHHRTKEFTEAFLETRELFKRLVDCTSENFVFFASSGTGAMEASVQNFF
NPGEKVLAVVGGKFGERWAELGRTWGLEVVELQVEWGKSVDPDQVEDILNKNPEIKGVLVQMSESSTGAYHDVKTLAKIT
KDRDTLLVVDAITALGVYNMKPHEWGLDVVVGGSQKAFMLPPGLSMLCSPKRRKKG
>Mature_216_residues
MKLFNFKGERVFTPGPVEIPDRIREVLGRQIIHHRTKEFTEAFLETRELFKRLVDCTSENFVFFASSGTGAMEASVQNFF
NPGEKVLAVVGGKFGERWAELGRTWGLEVVELQVEWGKSVDPDQVEDILNKNPEIKGVLVQMSESSTGAYHDVKTLAKIT
KDRDTLLVVDAITALGVYNMKPHEWGLDVVVGGSQKAFMLPPGLSMLCSPKRRKKG

Specific function: Soluble hydrogenase catalyzes both production and consumption of hydrogen from suitable artificial electron donors or acceptors. This subunit catalyzes the tritium-exchange activity [H]

COG id: COG0075

COG function: function code E; Serine-pyruvate aminotransferase/archaeal aspartate aminotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI4557289, Length=215, Percent_Identity=26.046511627907, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17536281, Length=200, Percent_Identity=31, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6321079, Length=214, Percent_Identity=30.8411214953271, Blast_Score=91, Evalue=1e-19,
Organism=Drosophila melanogaster, GI17530823, Length=201, Percent_Identity=25.3731343283582, Blast_Score=87, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: 2.6.1.-

Molecular weight: Translated: 24170; Mature: 24170

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLFNFKGERVFTPGPVEIPDRIREVLGRQIIHHRTKEFTEAFLETRELFKRLVDCTSEN
CCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
FVFFASSGTGAMEASVQNFFNPGEKVLAVVGGKFGERWAELGRTWGLEVVELQVEWGKSV
EEEEECCCCCHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEEEECCCCC
DPDQVEDILNKNPEIKGVLVQMSESSTGAYHDVKTLAKITKDRDTLLVVDAITALGVYNM
CHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEHHHHHCCCCC
KPHEWGLDVVVGGSQKAFMLPPGLSMLCSPKRRKKG
CCCCCCCEEEECCCCCEEECCCCCHHHCCCHHHCCC
>Mature Secondary Structure
MKLFNFKGERVFTPGPVEIPDRIREVLGRQIIHHRTKEFTEAFLETRELFKRLVDCTSEN
CCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
FVFFASSGTGAMEASVQNFFNPGEKVLAVVGGKFGERWAELGRTWGLEVVELQVEWGKSV
EEEEECCCCCHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEEEECCCCC
DPDQVEDILNKNPEIKGVLVQMSESSTGAYHDVKTLAKITKDRDTLLVVDAITALGVYNM
CHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEHHHHHCCCCC
KPHEWGLDVVVGGSQKAFMLPPGLSMLCSPKRRKKG
CCCCCCCEEEECCCCCEEECCCCCHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2129525 [H]