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The map label for this gene is gspE [H]

Identifier: 15606637

GI number: 15606637

Start: 1051345

End: 1052754

Strand: Direct

Name: gspE [H]

Synonym: aq_1474

Alternate gene names: 15606637

Gene position: 1051345-1052754 (Clockwise)

Preceding gene: 15606636

Following gene: 15606638

Centisome position: 67.77

GC content: 45.6

Gene sequence:

>1410_bases
ATGTCCGAATTGAGTGAAAGCTTTGAGTACATAGTAGAAGAAGAAAACGAGAGGGAACTCCTCGTAATCGTCCCTCAAAA
TTACAACTTTTTTGCAGTTGAGGAACTGAGATTTAAGAAGGGGAAGGACGTAAAACTAAAAGTTTTGAGCGAAGAGGAAT
ACGCAAAGCTCATTCAGGACAGGATTTCCGGGGAAAACGTAGTACTTGAAGAGGAAGAAGAGAGGGAAGAAAAGAGCGAG
GACATACTCTTCCAGAGGGACACATCACCTGCCGTAAGGCTGGTGAACACTGTTCTCATAAAAGCCTCTTCCGTTAACGC
CTCGGATGTCCACTTTGAACCTTACGAGGACGAAGCGGTAGTCAGGATAAGGATGGACGGAGTTCTCCACGACTATACTA
AGGTATCGCCTTCCCTCTATCAGGAAGTGGTATCCCGCATAAAAGTTATGGCAAAACTGAACGTTGCGGAAAAGAGGATA
CCGCAGGACGGCAAAATAAGGGTGAAGATAGGAAAGAGGGACTACGATATCAGAGTTTCCGTAGTGCCGACCGTTTTTGG
GGAAAGGGTAGTTCTGAGACTCCTTGAAAAGACTGGACAACTTTTAACACTCTCAGACCTCGGTTTATCGAAAGGAGACG
AAGAAAAGGTGAGAAGACTCGCGGAAAAGCCTTACGGAATAGTTCTCGTGACTGGTCCGACAGGTGCCGGAAAGTCCACT
ACTCTTTACGCTATGCTCCTTCACGTGAAAAACCCAAAGAAGAACATAATAACCATAGAAGATCCCGTGGAGTATCAGGT
AAAAGGGATAAGCCAGATTCAGGTAAACCCGAAAGTCGGACTCACCTTTGCACAGGGACTCAGGGCAATCCTGAGGCAGG
ATCCAGACGTCATAATGGTGGGAGAGATAAGGGACCCAGAGACGGCGGAGGTAGCAGTTCAGGCGGCATTAACGGGGCAC
TTAGTTTTATCGACCCTTCACACAAACGACGCACCTTCTGCAATTACGAGGTTAAAAGACCTAGGTATAGAACCTTTCCT
CATAGCCTCTTCCCTCGAAGGAGTTATAGCCCAAAGGCTTGTGAGAAAGATATGCAATAACTGCAAAGAGCCCTACAAAC
CCTCTAAGGAAGAAATAAGAGAACTGGGTCTTCCCGAGGAAGATTACACCTTCTACAGAGGAAGAGGTTGCGAACATTGC
CTCGGAACTGGCTACAGGGGTAGAACCGGAATTTTTGAAGTACTAGAAGTGGATGAAGATATGAAGAAATTGATAAACGA
AACTCAGGATTCTGTAAAAATAAGGCAATTTGCGAGGAAGAAGGGGTATAAAACTATGCTCGAAGATGGTATAGAAAAGA
TACTGAAAGGTATCACTACGAGCAGTGAAGTGCTGAGCGTTGTTAAGTGA

Upstream 100 bases:

>100_bases
ATAAGGAGCGGAGAGGATGCCTTCAGGATACTGCAAATGATAAGGAACGAACCTAACTTAAAAGTAGAGCTTATCAGAAG
AGGTAAAAGGGAGGAGATAA

Downstream 100 bases:

>100_bases
TGTTAAAATCTTTAACTATGGAAAACGTTAAGGTAGTTACGGGAGAAATAGAAAAGTTAAGGGAAAGGATTGAAAAGGTA
AAAGAAACTCTCGACCTTAT

Product: general secretion pathway protein E

Products: NA

Alternate protein names: Type II traffic warden ATPase [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MSELSESFEYIVEEENERELLVIVPQNYNFFAVEELRFKKGKDVKLKVLSEEEYAKLIQDRISGENVVLEEEEEREEKSE
DILFQRDTSPAVRLVNTVLIKASSVNASDVHFEPYEDEAVVRIRMDGVLHDYTKVSPSLYQEVVSRIKVMAKLNVAEKRI
PQDGKIRVKIGKRDYDIRVSVVPTVFGERVVLRLLEKTGQLLTLSDLGLSKGDEEKVRRLAEKPYGIVLVTGPTGAGKST
TLYAMLLHVKNPKKNIITIEDPVEYQVKGISQIQVNPKVGLTFAQGLRAILRQDPDVIMVGEIRDPETAEVAVQAALTGH
LVLSTLHTNDAPSAITRLKDLGIEPFLIASSLEGVIAQRLVRKICNNCKEPYKPSKEEIRELGLPEEDYTFYRGRGCEHC
LGTGYRGRTGIFEVLEVDEDMKKLINETQDSVKIRQFARKKGYKTMLEDGIEKILKGITTSSEVLSVVK

Sequences:

>Translated_469_residues
MSELSESFEYIVEEENERELLVIVPQNYNFFAVEELRFKKGKDVKLKVLSEEEYAKLIQDRISGENVVLEEEEEREEKSE
DILFQRDTSPAVRLVNTVLIKASSVNASDVHFEPYEDEAVVRIRMDGVLHDYTKVSPSLYQEVVSRIKVMAKLNVAEKRI
PQDGKIRVKIGKRDYDIRVSVVPTVFGERVVLRLLEKTGQLLTLSDLGLSKGDEEKVRRLAEKPYGIVLVTGPTGAGKST
TLYAMLLHVKNPKKNIITIEDPVEYQVKGISQIQVNPKVGLTFAQGLRAILRQDPDVIMVGEIRDPETAEVAVQAALTGH
LVLSTLHTNDAPSAITRLKDLGIEPFLIASSLEGVIAQRLVRKICNNCKEPYKPSKEEIRELGLPEEDYTFYRGRGCEHC
LGTGYRGRTGIFEVLEVDEDMKKLINETQDSVKIRQFARKKGYKTMLEDGIEKILKGITTSSEVLSVVK
>Mature_468_residues
SELSESFEYIVEEENERELLVIVPQNYNFFAVEELRFKKGKDVKLKVLSEEEYAKLIQDRISGENVVLEEEEEREEKSED
ILFQRDTSPAVRLVNTVLIKASSVNASDVHFEPYEDEAVVRIRMDGVLHDYTKVSPSLYQEVVSRIKVMAKLNVAEKRIP
QDGKIRVKIGKRDYDIRVSVVPTVFGERVVLRLLEKTGQLLTLSDLGLSKGDEEKVRRLAEKPYGIVLVTGPTGAGKSTT
LYAMLLHVKNPKKNIITIEDPVEYQVKGISQIQVNPKVGLTFAQGLRAILRQDPDVIMVGEIRDPETAEVAVQAALTGHL
VLSTLHTNDAPSAITRLKDLGIEPFLIASSLEGVIAQRLVRKICNNCKEPYKPSKEEIRELGLPEEDYTFYRGRGCEHCL
GTGYRGRTGIFEVLEVDEDMKKLINETQDSVKIRQFARKKGYKTMLEDGIEKILKGITTSSEVLSVVK

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of a variety of enzymes across the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=410, Percent_Identity=47.3170731707317, Blast_Score=406, Evalue=1e-114,
Organism=Escherichia coli, GI1786296, Length=404, Percent_Identity=39.6039603960396, Blast_Score=323, Evalue=2e-89,
Organism=Escherichia coli, GI87082188, Length=145, Percent_Identity=48.2758620689655, Blast_Score=118, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 52907; Mature: 52775

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELSESFEYIVEEENERELLVIVPQNYNFFAVEELRFKKGKDVKLKVLSEEEYAKLIQD
CCHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEHHHHCCCCCEEEEEECCHHHHHHHHH
RISGENVVLEEEEEREEKSEDILFQRDTSPAVRLVNTVLIKASSVNASDVHFEPYEDEAV
HCCCCCEEECCHHHHHHHHHCCEEECCCCHHHHHHHHHHEEECCCCCCCCCCCCCCCCEE
VRIRMDGVLHDYTKVSPSLYQEVVSRIKVMAKLNVAEKRIPQDGKIRVKIGKRDYDIRVS
EEEEECCHHHHHHHCCHHHHHHHHHHHHHHHHHCHHHHHCCCCCEEEEEECCCCCEEEEE
VVPTVFGERVVLRLLEKTGQLLTLSDLGLSKGDEEKVRRLAEKPYGIVLVTGPTGAGKST
EECHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCC
TLYAMLLHVKNPKKNIITIEDPVEYQVKGISQIQVNPKVGLTFAQGLRAILRQDPDVIMV
EEEEEEEEECCCCCCEEEECCCCCEEECCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEE
GEIRDPETAEVAVQAALTGHLVLSTLHTNDAPSAITRLKDLGIEPFLIASSLEGVIAQRL
ECCCCCCHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHH
VRKICNNCKEPYKPSKEEIRELGLPEEDYTFYRGRGCEHCLGTGYRGRTGIFEVLEVDED
HHHHHHHCCCCCCCCHHHHHHCCCCCCCCCEECCCCCCHHHCCCCCCCCCHHHHHHCCHH
MKKLINETQDSVKIRQFARKKGYKTMLEDGIEKILKGITTSSEVLSVVK
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHCC
>Mature Secondary Structure 
SELSESFEYIVEEENERELLVIVPQNYNFFAVEELRFKKGKDVKLKVLSEEEYAKLIQD
CHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEHHHHCCCCCEEEEEECCHHHHHHHHH
RISGENVVLEEEEEREEKSEDILFQRDTSPAVRLVNTVLIKASSVNASDVHFEPYEDEAV
HCCCCCEEECCHHHHHHHHHCCEEECCCCHHHHHHHHHHEEECCCCCCCCCCCCCCCCEE
VRIRMDGVLHDYTKVSPSLYQEVVSRIKVMAKLNVAEKRIPQDGKIRVKIGKRDYDIRVS
EEEEECCHHHHHHHCCHHHHHHHHHHHHHHHHHCHHHHHCCCCCEEEEEECCCCCEEEEE
VVPTVFGERVVLRLLEKTGQLLTLSDLGLSKGDEEKVRRLAEKPYGIVLVTGPTGAGKST
EECHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCC
TLYAMLLHVKNPKKNIITIEDPVEYQVKGISQIQVNPKVGLTFAQGLRAILRQDPDVIMV
EEEEEEEEECCCCCCEEEECCCCCEEECCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEE
GEIRDPETAEVAVQAALTGHLVLSTLHTNDAPSAITRLKDLGIEPFLIASSLEGVIAQRL
ECCCCCCHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHH
VRKICNNCKEPYKPSKEEIRELGLPEEDYTFYRGRGCEHCLGTGYRGRTGIFEVLEVDED
HHHHHHHCCCCCCCCHHHHHHCCCCCCCCCEECCCCCCHHHCCCCCCCCCHHHHHHCCHH
MKKLINETQDSVKIRQFARKKGYKTMLEDGIEKILKGITTSSEVLSVVK
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1588814; 10984043 [H]