Click here to switch to the map view.

The map label for this gene is pdxJ

Identifier: 15606602

GI number: 15606602

Start: 1014723

End: 1015451

Strand: Direct

Name: pdxJ

Synonym: aq_1423

Alternate gene names: 15606602

Gene position: 1014723-1015451 (Clockwise)

Preceding gene: 15606600

Following gene: 15606604

Centisome position: 65.41

GC content: 47.05

Gene sequence:

>729_bases
ATGAGACTCGGAGTAAACGTTGACCACGTGGCCACCGTAAGGCAGGCGAGGAGAACCTTTGAGCCCTCCCCCGTATTCGC
GGCCCTCATAGCCCAGCAGGCGGGAGCGGATCAGATAACCCTCCATCTCAGGGAGGACAGGAGACACATACAGGACAGGG
ATTTAGAACTCATAAAGGAACTAATAACCATTCCCGTAAACCTTGAGATGGCTCCCACTGAAGAGATGAGAGAAATAGCC
CTGAGAGTAAAACCTGACAGAATAACCCTTGTTCCCGAAAGAAGGGAGGAAATCACCACCGAAGGTGGTCTTGACGTAGT
ATCACTGAAGGAAAAACTCAAAGAATACTTAAAGCCCATAAAGGAGGCAGGTATTGAAGTCTCCCTCTTTATAGAAGCTC
AGAAGGAACAAATAGACGCAAGTGTAGAAGTAGGGGCTGACGCCATAGAAATCCACACAGGAAGGTACGCAAACCTCTGG
AACGAACACAGGTTTGAGGAAGCGAAGGAAGAGCTAAACAGGATAAAGGAAGCGGCAATTTACGCGAAGGAAAAAGGACT
GAAGGTTTACGCGGGACACGGACTTACTTACCACAACGTAAAAGACTTTGTCAGGGAACTCAAAGGCTACGTAGAAGAAT
TAAACATCGGTCACTCAATTGTGGCAAACGCCGTGATATTCGGATTTGAAAGAGCTGTGAAGGAGATGTTAAACCTTATT
AAGACTTGA

Upstream 100 bases:

>100_bases
GGAATTCAAGGATATCGGCCATTCTCTCAAAGATTTTTGCAAGTTCCTGATTCTTTCTGGACATACTTTTAAAGTTAAGC
CTTGAATTAAGATTTAAAGT

Downstream 100 bases:

>100_bases
CTCGTATTTTCTCAAAACTTCGGTTATTAATTCGTTGGATATTAAAAGGTGCTCTTCCTTTATCCTCAGCCTTCCTCCTT
TTACTTCAAAGAAATCCCTC

Product: pyridoxine 5'-phosphate synthase

Products: NA

Alternate protein names: PNP synthase

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MRLGVNVDHVATVRQARRTFEPSPVFAALIAQQAGADQITLHLREDRRHIQDRDLELIKELITIPVNLEMAPTEEMREIA
LRVKPDRITLVPERREEITTEGGLDVVSLKEKLKEYLKPIKEAGIEVSLFIEAQKEQIDASVEVGADAIEIHTGRYANLW
NEHRFEEAKEELNRIKEAAIYAKEKGLKVYAGHGLTYHNVKDFVRELKGYVEELNIGHSIVANAVIFGFERAVKEMLNLI
KT

Sequences:

>Translated_242_residues
MRLGVNVDHVATVRQARRTFEPSPVFAALIAQQAGADQITLHLREDRRHIQDRDLELIKELITIPVNLEMAPTEEMREIA
LRVKPDRITLVPERREEITTEGGLDVVSLKEKLKEYLKPIKEAGIEVSLFIEAQKEQIDASVEVGADAIEIHTGRYANLW
NEHRFEEAKEELNRIKEAAIYAKEKGLKVYAGHGLTYHNVKDFVRELKGYVEELNIGHSIVANAVIFGFERAVKEMLNLI
KT
>Mature_242_residues
MRLGVNVDHVATVRQARRTFEPSPVFAALIAQQAGADQITLHLREDRRHIQDRDLELIKELITIPVNLEMAPTEEMREIA
LRVKPDRITLVPERREEITTEGGLDVVSLKEKLKEYLKPIKEAGIEVSLFIEAQKEQIDASVEVGADAIEIHTGRYANLW
NEHRFEEAKEELNRIKEAAIYAKEKGLKVYAGHGLTYHNVKDFVRELKGYVEELNIGHSIVANAVIFGFERAVKEMLNLI
KT

Specific function: Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate

COG id: COG0854

COG function: function code H; Pyridoxal phosphate biosynthesis protein

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP synthase family

Homologues:

Organism=Escherichia coli, GI1788917, Length=238, Percent_Identity=54.6218487394958, Blast_Score=250, Evalue=7e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXJ_AQUAE (O67417)

Other databases:

- EMBL:   AE000657
- PIR:   G70423
- RefSeq:   NP_213982.1
- ProteinModelPortal:   O67417
- SMR:   O67417
- GeneID:   1192948
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_1423
- NMPDR:   fig|224324.1.peg.997
- HOGENOM:   HBG299314
- OMA:   AGHGLNY
- PhylomeDB:   O67417
- ProtClustDB:   PRK05265
- BioCyc:   AAEO224324:AQ_1423-MONOMER
- BRENDA:   2.6.99.2
- GO:   GO:0005737
- HAMAP:   MF_00279
- InterPro:   IPR013785
- InterPro:   IPR004569
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00559

Pfam domain/function: PF03740 PdxJ; SSF63892 PyrdxlP_synth_PdxJ

EC number: =2.6.99.2

Molecular weight: Translated: 27598; Mature: 27598

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: NA

Important sites: ACT_SITE 42-42 ACT_SITE 69-69 ACT_SITE 193-193 BINDING 6-6 BINDING 17-17 BINDING 44-44 BINDING 49-49 BINDING 99-99 BINDING 194-194

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLGVNVDHVATVRQARRTFEPSPVFAALIAQQAGADQITLHLREDRRHIQDRDLELIKE
CCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEEHHHHHHHHHCCHHHHHH
LITIPVNLEMAPTEEMREIALRVKPDRITLVPERREEITTEGGLDVVSLKEKLKEYLKPI
HHHCCCCCEECCHHHHHHHHHEECCCEEEECCCHHHHHCCCCCCHHHHHHHHHHHHHHHH
KEAGIEVSLFIEAQKEQIDASVEVGADAIEIHTGRYANLWNEHRFEEAKEELNRIKEAAI
HHCCCEEEEEEEEHHHHCCCHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
YAKEKGLKVYAGHGLTYHNVKDFVRELKGYVEELNIGHSIVANAVIFGFERAVKEMLNLI
HHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
KT
CC
>Mature Secondary Structure
MRLGVNVDHVATVRQARRTFEPSPVFAALIAQQAGADQITLHLREDRRHIQDRDLELIKE
CCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEEHHHHHHHHHCCHHHHHH
LITIPVNLEMAPTEEMREIALRVKPDRITLVPERREEITTEGGLDVVSLKEKLKEYLKPI
HHHCCCCCEECCHHHHHHHHHEECCCEEEECCCHHHHHCCCCCCHHHHHHHHHHHHHHHH
KEAGIEVSLFIEAQKEQIDASVEVGADAIEIHTGRYANLWNEHRFEEAKEELNRIKEAAI
HHCCCEEEEEEEEHHHHCCCHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
YAKEKGLKVYAGHGLTYHNVKDFVRELKGYVEELNIGHSIVANAVIFGFERAVKEMLNLI
HHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
KT
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320