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The map label for this gene is ispD

Identifier: 15606527

GI number: 15606527

Start: 938284

End: 938925

Strand: Direct

Name: ispD

Synonym: aq_1323

Alternate gene names: 15606527

Gene position: 938284-938925 (Clockwise)

Preceding gene: 15606526

Following gene: 15606530

Centisome position: 60.48

GC content: 45.79

Gene sequence:

>642_bases
ATGTACACGGCAATAATCCTCGCTGCTGGTAGAGGTAGCAGAATAGGTTTCAGGAAACAGTTTGCAACCCTTTGCGGAAA
GCCTCTATTTATGCACTCCCTTGAGAAAGTTCTGGACATATTTGAAGAAGTAATACTCGTTCTCCCAGAGGATTTCTTAG
ATAAAGTTAAAGTTCACCCGAAAGTGAAGAAAGTAGCGGGAGGACCAGAAAGACAGGACTCTGTATTTAACGCATTGTTA
CAGGCCACAGGAGACATCGTAGTAATACACGACTCCGCAAGACCACTCGCTACAAAAAAGATGTTTTTAGAAGTAGCCCA
GCTCGGGGATTACCACGGGAAAGTGGTGGCTTCTCCTGCAAGGGATACTTTAAAGGAAGTAGTAGAAGGTAAGGTGATAA
AAACTTTAAATCGCTCTTTAATCTGGCACGCCCAGACACCCCAGGCCTTCAGGAGGGATATTCTCCTCGAGTGTCACATG
AGGGCAAAGGCAGAGGGTTTCGTCGGAACGGACGACGCAAGCCTGCTGGAACGCTACGGCTACAGCGTGGGAGTTGTTGA
AGGAAGCTACTGGAATGTCAAGATAACTTATCCTGAGGATTTAGAAATGGTGAAAAAGATAATGGGATGCGAAGAGGATT
AA

Upstream 100 bases:

>100_bases
TTTCACGGTTCTCGGTACGGCTTTCCTCGTTATATTCTTTGGTCTATTTCCCCATGTGGTTCTTGACTTTATCCTGCGTG
CCCTCTCTTAATTAATTTTT

Downstream 100 bases:

>100_bases
CCCGTTTCTTCCGCCCTCGGGAATTGCGGGCAGGCGTCTTCGGGCGGTATTTCTTTTGGAAACTCTTCTTTATACTCGTT
GTGATAACCGCAGACGTAGC

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MYTAIILAAGRGSRIGFRKQFATLCGKPLFMHSLEKVLDIFEEVILVLPEDFLDKVKVHPKVKKVAGGPERQDSVFNALL
QATGDIVVIHDSARPLATKKMFLEVAQLGDYHGKVVASPARDTLKEVVEGKVIKTLNRSLIWHAQTPQAFRRDILLECHM
RAKAEGFVGTDDASLLERYGYSVGVVEGSYWNVKITYPEDLEMVKKIMGCEED

Sequences:

>Translated_213_residues
MYTAIILAAGRGSRIGFRKQFATLCGKPLFMHSLEKVLDIFEEVILVLPEDFLDKVKVHPKVKKVAGGPERQDSVFNALL
QATGDIVVIHDSARPLATKKMFLEVAQLGDYHGKVVASPARDTLKEVVEGKVIKTLNRSLIWHAQTPQAFRRDILLECHM
RAKAEGFVGTDDASLLERYGYSVGVVEGSYWNVKITYPEDLEMVKKIMGCEED
>Mature_213_residues
MYTAIILAAGRGSRIGFRKQFATLCGKPLFMHSLEKVLDIFEEVILVLPEDFLDKVKVHPKVKKVAGGPERQDSVFNALL
QATGDIVVIHDSARPLATKKMFLEVAQLGDYHGKVVASPARDTLKEVVEGKVIKTLNRSLIWHAQTPQAFRRDILLECHM
RAKAEGFVGTDDASLLERYGYSVGVVEGSYWNVKITYPEDLEMVKKIMGCEED

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Homo sapiens, GI157412259, Length=233, Percent_Identity=27.8969957081545, Blast_Score=64, Evalue=9e-11,
Organism=Escherichia coli, GI1789104, Length=216, Percent_Identity=37.037037037037, Blast_Score=117, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_AQUAE (O67343)

Other databases:

- EMBL:   AE000657
- PIR:   D70414
- RefSeq:   NP_213907.1
- ProteinModelPortal:   O67343
- SMR:   O67343
- GeneID:   1192781
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_1323
- NMPDR:   fig|224324.1.peg.922
- HOGENOM:   HBG672839
- OMA:   SKVIVVC
- PhylomeDB:   O67343
- ProtClustDB:   PRK00155
- BioCyc:   AAEO224324:AQ_1323-MONOMER
- BRENDA:   2.7.7.60
- HAMAP:   MF_00108
- InterPro:   IPR001228
- InterPro:   IPR018294
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 23819; Mature: 23819

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYTAIILAAGRGSRIGFRKQFATLCGKPLFMHSLEKVLDIFEEVILVLPEDFLDKVKVHP
CCEEEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCC
KVKKVAGGPERQDSVFNALLQATGDIVVIHDSARPLATKKMFLEVAQLGDYHGKVVASPA
HHHHHCCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEECCH
RDTLKEVVEGKVIKTLNRSLIWHAQTPQAFRRDILLECHMRAKAEGFVGTDDASLLERYG
HHHHHHHHCCHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCC
YSVGVVEGSYWNVKITYPEDLEMVKKIMGCEED
CEEEEEECCEEEEEEECCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MYTAIILAAGRGSRIGFRKQFATLCGKPLFMHSLEKVLDIFEEVILVLPEDFLDKVKVHP
CCEEEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCC
KVKKVAGGPERQDSVFNALLQATGDIVVIHDSARPLATKKMFLEVAQLGDYHGKVVASPA
HHHHHCCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEECCH
RDTLKEVVEGKVIKTLNRSLIWHAQTPQAFRRDILLECHMRAKAEGFVGTDDASLLERYG
HHHHHHHHCCHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCC
YSVGVVEGSYWNVKITYPEDLEMVKKIMGCEED
CEEEEEECCEEEEEEECCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320