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The map label for this gene is nuoI1

Identifier: 15606521

GI number: 15606521

Start: 932142

End: 932747

Strand: Direct

Name: nuoI1

Synonym: aq_1317

Alternate gene names: 15606521

Gene position: 932142-932747 (Clockwise)

Preceding gene: 15606520

Following gene: 15606522

Centisome position: 60.09

GC content: 46.04

Gene sequence:

>606_bases
ATGGGTGTGAAGAAGTTAAGCAGGAAGGATTACTTGAACATACTAGAAAGCATACTTTTTATAGACTTCCTTAAGGGACT
TTCCGTAACGCTGAAAAACCTCTTGAGGAGACCAATTACTACGGAATACCCGAAAGAAAAACTCACTCCTCCCAAGCGTT
TCAGGGGAGCTCACGGGCACTATGTGTGGGACGGAACCGAACCTGACTCACTCAAAGCTATAGAAAAGTTCATGTCTTAT
GAAAAGGCAAAGAGCAGGTGTGTTGCCTGCTACATGTGTCAGACAGCCTGTCCCATGCCAACTCTTTTCAGAATAGAAGC
GGTGCAGCTTCCTAATGGGAAGAAAAAGGTCGTAAGGTTTGACATGAACCTCCTTAACTGTCTCTTCTGTGGGCTCTGCG
TTGACGCGTGTCCCGTGGGATGTCTTACGATGACGGATATCTTTGAACTCGCAAATTACTCAAGGAGAAATGAAGTCCTT
CGCATGGAGGACCTTGAGAAGTTCGCCATAGACTTCAAGCAAAGAAGGGGCAACGAGCCCGACAGAATTTGGCCAAATGA
TGAAGAAAGGGAAAAACTGTGGGGGAAGATAGAGTGGAGTGGTTAA

Upstream 100 bases:

>100_bases
AGGACATTACGGAAATTGCCTGGAAGATACTCCTTCCCATAGCGATACTTAACGTGATAATTACTGCGGTTGTGGTTTAT
GCATTTTAGGAGGATTTAGG

Downstream 100 bases:

>100_bases
TCTTCTTCATCTTAGGAGCTTGGCTTATAATTTCTGCCTTGGGGACGGTTCTGGCTAAAAATCCCATTCACGTTATGCTC
TTCTTCCTTTCCTCCTTACT

Product: NADH dehydrogenase I chain I

Products: NA

Alternate protein names: NADH dehydrogenase I subunit I; NDH-1 subunit I

Number of amino acids: Translated: 201; Mature: 200

Protein sequence:

>201_residues
MGVKKLSRKDYLNILESILFIDFLKGLSVTLKNLLRRPITTEYPKEKLTPPKRFRGAHGHYVWDGTEPDSLKAIEKFMSY
EKAKSRCVACYMCQTACPMPTLFRIEAVQLPNGKKKVVRFDMNLLNCLFCGLCVDACPVGCLTMTDIFELANYSRRNEVL
RMEDLEKFAIDFKQRRGNEPDRIWPNDEEREKLWGKIEWSG

Sequences:

>Translated_201_residues
MGVKKLSRKDYLNILESILFIDFLKGLSVTLKNLLRRPITTEYPKEKLTPPKRFRGAHGHYVWDGTEPDSLKAIEKFMSY
EKAKSRCVACYMCQTACPMPTLFRIEAVQLPNGKKKVVRFDMNLLNCLFCGLCVDACPVGCLTMTDIFELANYSRRNEVL
RMEDLEKFAIDFKQRRGNEPDRIWPNDEEREKLWGKIEWSG
>Mature_200_residues
GVKKLSRKDYLNILESILFIDFLKGLSVTLKNLLRRPITTEYPKEKLTPPKRFRGAHGHYVWDGTEPDSLKAIEKFMSYE
KAKSRCVACYMCQTACPMPTLFRIEAVQLPNGKKKVVRFDMNLLNCLFCGLCVDACPVGCLTMTDIFELANYSRRNEVLR
MEDLEKFAIDFKQRRGNEPDRIWPNDEEREKLWGKIEWSG

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Homo sapiens, GI4505371, Length=149, Percent_Identity=29.5302013422819, Blast_Score=86, Evalue=2e-17,
Organism=Escherichia coli, GI1788617, Length=126, Percent_Identity=31.7460317460317, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17555194, Length=151, Percent_Identity=29.1390728476821, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17864306, Length=149, Percent_Identity=28.8590604026846, Blast_Score=88, Evalue=3e-18,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOI1_AQUAE (O67337)

Other databases:

- EMBL:   AE000657
- PIR:   F70413
- RefSeq:   NP_213901.1
- ProteinModelPortal:   O67337
- SMR:   O67337
- GeneID:   1192775
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_1317
- NMPDR:   fig|224324.1.peg.916
- HOGENOM:   HBG724359
- OMA:   VEGGDNT
- ProtClustDB:   CLSK233603
- BioCyc:   AAEO224324:AQ_1317-MONOMER
- BRENDA:   1.6.99.5
- HAMAP:   MF_01351
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR010226
- Gene3D:   G3DSA:1.10.1060.10

Pfam domain/function: SSF46548 Helical_ferredxn

EC number: =1.6.99.5

Molecular weight: Translated: 23411; Mature: 23279

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
8.0 %Cys+Met (Translated Protein)
4.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
7.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVKKLSRKDYLNILESILFIDFLKGLSVTLKNLLRRPITTEYPKEKLTPPKRFRGAHGH
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCHHHHCCCCCC
YVWDGTEPDSLKAIEKFMSYEKAKSRCVACYMCQTACPMPTLFRIEAVQLPNGKKKVVRF
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEEECCCCCHHHHHH
DMNLLNCLFCGLCVDACPVGCLTMTDIFELANYSRRNEVLRMEDLEKFAIDFKQRRGNEP
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
DRIWPNDEEREKLWGKIEWSG
CCCCCCCHHHHHHCCCCCCCC
>Mature Secondary Structure 
GVKKLSRKDYLNILESILFIDFLKGLSVTLKNLLRRPITTEYPKEKLTPPKRFRGAHGH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCHHHHCCCCCC
YVWDGTEPDSLKAIEKFMSYEKAKSRCVACYMCQTACPMPTLFRIEAVQLPNGKKKVVRF
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEEECCCCCHHHHHH
DMNLLNCLFCGLCVDACPVGCLTMTDIFELANYSRRNEVLRMEDLEKFAIDFKQRRGNEP
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
DRIWPNDEEREKLWGKIEWSG
CCCCCCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9537320