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The map label for this gene is argG

Identifier: 15606397

GI number: 15606397

Start: 797533

End: 798738

Strand: Direct

Name: argG

Synonym: aq_1140

Alternate gene names: 15606397

Gene position: 797533-798738 (Clockwise)

Preceding gene: 15606396

Following gene: 15606398

Centisome position: 51.41

GC content: 48.67

Gene sequence:

>1206_bases
ATGAAGAAAAAGAGGGTAATTCTCGCATACTCGGGCGGACTCGACACATCCATAATAGTGAGATGGCTCACGGAAAAAGG
GTACGAGGTAATAACATACACCGCGGACGTGGGACAGGGAGAAGAACTCTCTGAAATTCCCGAAAAGGCAAGGAGAGCGG
GAGCGATTGAAGCCATAGTAGAGGATTTAAAAGAAACCTTTGCGGAAAATTACTGCCTTCCAACTTTGAGGGCATTAGCC
CTTTACGAAGGGAAGTACCCCCTCACCGCAGCCCTCTCAAGACCTCTCATAGCAGAGAGGCTCGTTTACTATGCCGAAAA
GTTCAACGCGGACTACGTAGCACACGGCTCAACTGGGAAGGGAAACGATCAGGTGAGGTTTGAACTCTCAGTCTGGGCCC
TAAACCCCGATATAGAGGTTCTCGCTCCCGTGAGAGAATGGGAGTTCAAATCAAGGGAAGAACAAGTTGAGTACGCTCAG
AGGTTCAACATCCCCGTTAAAGCTACGAAAGAAAAGCCATACTCCATAGACAGGAACCTCTGGGGCGTTTCCATAGAGTG
CGGACCCTTAGAAGACCCGTGGCAAGAACCACCGCAAGACGCTTACCAGATCACCCAATCCCCTGAAGAGGCTCCGGATG
AACCAGAATACGTTACCGTGGGTTTTGAAAAAGGAAAACCCGTTTACCTGAACGGTGAGCGCTACGAAGAGCAGTGGAAA
TTGATAGCCAACCTAAATGAAATAGCGGGAAGACACGGTGTGGGAAGGATAGACATGGTTGAAAACAGACTCGTAGGGAT
AAAGAGCAGGGAGATATACGAAGCTCCCGGAGCCATGGTTCTCTACGAAGCATACAGGGATTTACTCTCTTTAGTTCTTG
ACAGGTTTACCTTCCACTACTTCCTCACCCATATTCCCCACGAGTACGCAAAGCTCGTTTACGAAGGTCTCTGGTTCACC
CCCTTGAGGGAAGCGCTTGACGCCTTTACGAATAAAATCGCCGAGTTCGCAACGGGAGAAGTAAGATTAAAACTCTACAA
AGGAAGTGTAAGCGTTGTTGGGAGACGTTCACCCAACTCCCTTTACGTTGAAGAGCTCGCAACTTACTCGGAAAAGGACC
AATTTGACCAAATAGCAGGTAAGCACTTCACAAAGGTATGGGGACTTCCACTGAAGGTTCTGGGAAGGGTGAGAAAAGGT
AAATAG

Upstream 100 bases:

>100_bases
TATAAGCTACGGAGTCAGTAATTTGCTTTCCAATATGATAATGCTGGGAATAGTTGGTTCTATATACAGGAGAAACAGCG
ATGTATTAAACTTATGAATT

Downstream 100 bases:

>100_bases
AAATTTAACAATTAAAGACTTAACTTAATAGTATGAACGTAGGAATATTTAGTAGATGGAACGCAACGTGCGGGGTTTCC
CTGCACGCGGAAATGATAGG

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MKKKRVILAYSGGLDTSIIVRWLTEKGYEVITYTADVGQGEELSEIPEKARRAGAIEAIVEDLKETFAENYCLPTLRALA
LYEGKYPLTAALSRPLIAERLVYYAEKFNADYVAHGSTGKGNDQVRFELSVWALNPDIEVLAPVREWEFKSREEQVEYAQ
RFNIPVKATKEKPYSIDRNLWGVSIECGPLEDPWQEPPQDAYQITQSPEEAPDEPEYVTVGFEKGKPVYLNGERYEEQWK
LIANLNEIAGRHGVGRIDMVENRLVGIKSREIYEAPGAMVLYEAYRDLLSLVLDRFTFHYFLTHIPHEYAKLVYEGLWFT
PLREALDAFTNKIAEFATGEVRLKLYKGSVSVVGRRSPNSLYVEELATYSEKDQFDQIAGKHFTKVWGLPLKVLGRVRKG
K

Sequences:

>Translated_401_residues
MKKKRVILAYSGGLDTSIIVRWLTEKGYEVITYTADVGQGEELSEIPEKARRAGAIEAIVEDLKETFAENYCLPTLRALA
LYEGKYPLTAALSRPLIAERLVYYAEKFNADYVAHGSTGKGNDQVRFELSVWALNPDIEVLAPVREWEFKSREEQVEYAQ
RFNIPVKATKEKPYSIDRNLWGVSIECGPLEDPWQEPPQDAYQITQSPEEAPDEPEYVTVGFEKGKPVYLNGERYEEQWK
LIANLNEIAGRHGVGRIDMVENRLVGIKSREIYEAPGAMVLYEAYRDLLSLVLDRFTFHYFLTHIPHEYAKLVYEGLWFT
PLREALDAFTNKIAEFATGEVRLKLYKGSVSVVGRRSPNSLYVEELATYSEKDQFDQIAGKHFTKVWGLPLKVLGRVRKG
K
>Mature_401_residues
MKKKRVILAYSGGLDTSIIVRWLTEKGYEVITYTADVGQGEELSEIPEKARRAGAIEAIVEDLKETFAENYCLPTLRALA
LYEGKYPLTAALSRPLIAERLVYYAEKFNADYVAHGSTGKGNDQVRFELSVWALNPDIEVLAPVREWEFKSREEQVEYAQ
RFNIPVKATKEKPYSIDRNLWGVSIECGPLEDPWQEPPQDAYQITQSPEEAPDEPEYVTVGFEKGKPVYLNGERYEEQWK
LIANLNEIAGRHGVGRIDMVENRLVGIKSREIYEAPGAMVLYEAYRDLLSLVLDRFTFHYFLTHIPHEYAKLVYEGLWFT
PLREALDAFTNKIAEFATGEVRLKLYKGSVSVVGRRSPNSLYVEELATYSEKDQFDQIAGKHFTKVWGLPLKVLGRVRKG
K

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=404, Percent_Identity=41.8316831683168, Blast_Score=309, Evalue=3e-84,
Organism=Homo sapiens, GI16950633, Length=404, Percent_Identity=41.8316831683168, Blast_Score=309, Evalue=3e-84,
Organism=Escherichia coli, GI1789563, Length=383, Percent_Identity=32.1148825065274, Blast_Score=159, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6324514, Length=414, Percent_Identity=36.7149758454106, Blast_Score=269, Evalue=5e-73,
Organism=Drosophila melanogaster, GI21358151, Length=384, Percent_Identity=40.1041666666667, Blast_Score=278, Evalue=5e-75,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_AQUAE (O67213)

Other databases:

- EMBL:   AE000657
- PIR:   B70398
- RefSeq:   NP_213777.1
- ProteinModelPortal:   O67213
- SMR:   O67213
- GeneID:   1192559
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_1140
- NMPDR:   fig|224324.1.peg.792
- HOGENOM:   HBG335267
- OMA:   KRVILAY
- PhylomeDB:   O67213
- ProtClustDB:   PRK00509
- BioCyc:   AAEO224324:AQ_1140-MONOMER
- BRENDA:   6.3.4.5
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 45849; Mature: 45849

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 35-35 BINDING 86-86 BINDING 116-116 BINDING 118-118 BINDING 122-122 BINDING 122-122 BINDING 123-123 BINDING 126-126 BINDING 175-175 BINDING 184-184 BINDING 261-261 BINDING 273-273

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKRVILAYSGGLDTSIIVRWLTEKGYEVITYTADVGQGEELSEIPEKARRAGAIEAIV
CCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHCCHHHHHH
EDLKETFAENYCLPTLRALALYEGKYPLTAALSRPLIAERLVYYAEKFNADYVAHGSTGK
HHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHCHHHHHHHHHHHHHCCCCEEEECCCCC
GNDQVRFELSVWALNPDIEVLAPVREWEFKSREEQVEYAQRFNIPVKATKEKPYSIDRNL
CCCEEEEEEEEEEECCCCEEEECCHHHHHCCHHHHHHHHHHCCCCEEECCCCCCCCCCCE
WGVSIECGPLEDPWQEPPQDAYQITQSPEEAPDEPEYVTVGFEKGKPVYLNGERYEEQWK
EEEEEECCCCCCCCCCCCHHHHHHCCCCCCCCCCCCEEEEEECCCCEEEECCHHHHHHHH
LIANLNEIAGRHGVGRIDMVENRLVGIKSREIYEAPGAMVLYEAYRDLLSLVLDRFTFHY
HHHHHHHHHCCCCCCCEEHHHCHHCCCCCCHHHCCCCCEEHHHHHHHHHHHHHHHHHHHH
FLTHIPHEYAKLVYEGLWFTPLREALDAFTNKIAEFATGEVRLKLYKGSVSVVGRRSPNS
HHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCEEEEECCCCCC
LYVEELATYSEKDQFDQIAGKHFTKVWGLPLKVLGRVRKGK
HHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCC
>Mature Secondary Structure
MKKKRVILAYSGGLDTSIIVRWLTEKGYEVITYTADVGQGEELSEIPEKARRAGAIEAIV
CCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHCCHHHHHH
EDLKETFAENYCLPTLRALALYEGKYPLTAALSRPLIAERLVYYAEKFNADYVAHGSTGK
HHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHCHHHHHHHHHHHHHCCCCEEEECCCCC
GNDQVRFELSVWALNPDIEVLAPVREWEFKSREEQVEYAQRFNIPVKATKEKPYSIDRNL
CCCEEEEEEEEEEECCCCEEEECCHHHHHCCHHHHHHHHHHCCCCEEECCCCCCCCCCCE
WGVSIECGPLEDPWQEPPQDAYQITQSPEEAPDEPEYVTVGFEKGKPVYLNGERYEEQWK
EEEEEECCCCCCCCCCCCHHHHHHCCCCCCCCCCCCEEEEEECCCCEEEECCHHHHHHHH
LIANLNEIAGRHGVGRIDMVENRLVGIKSREIYEAPGAMVLYEAYRDLLSLVLDRFTFHY
HHHHHHHHHCCCCCCCEEHHHCHHCCCCCCHHHCCCCCEEHHHHHHHHHHHHHHHHHHHH
FLTHIPHEYAKLVYEGLWFTPLREALDAFTNKIAEFATGEVRLKLYKGSVSVVGRRSPNS
HHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCEEEEECCCCCC
LYVEELATYSEKDQFDQIAGKHFTKVWGLPLKVLGRVRKGK
HHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320