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The map label for this gene is ppnK

Identifier: 15606239

GI number: 15606239

Start: 627058

End: 627882

Strand: Direct

Name: ppnK

Synonym: aq_909

Alternate gene names: 15606239

Gene position: 627058-627882 (Clockwise)

Preceding gene: 15606238

Following gene: 15606243

Centisome position: 40.42

GC content: 43.76

Gene sequence:

>825_bases
ATGCGAAAGGTCCTAGTCTTTTTAAAGAATTCCAAAAAGGCATTTGAGACTTTTAAAAGAGTTGAAAGAGTTTTAAAAGA
TTTAAACCTCAGCTACAAAAAGTTTATAAACAGGAAGGAACTTTTTAAAGTTTTAAAACCTAAGGATTATGAACTCTTTC
TCGTTATAGGGGGAGATGGTACATTCTTGAGTGCCGCTCGCATCGCTTCGCGTTTCGGTGTTCCCCTTGTTGGTGTAAAC
GAAGGGAGGTTCGGTTTTCTCACAGAAATAAAGAAGGAAGAAATAAAAAAAGTTCTGCCGCTGGTACTGGAAGGAAGGGC
GAAACTTCAGGAGAGGTTGATGATAGACGTTTACCTGAGGAGCAGAAACAGGTTGAGGTATCTCGGGAATTACCTGAACG
ACGCCGTGATTTCAAAGAGCAGTATAGCGAGGATAATAAGGACTAAGGTGTTTATAAATGGGGAGGAAGTTCTGGAGGTC
TTCGGAGACGGTGTTATCCTTTCCACACCTACGGGTTCAACCGCCTACGCACTTTCCGCGGGAGGACCGATTGTTTACCC
CGAATCTCAGAACTTACTCTTCGTTCCCATATGCCCCCATACGCTTTCAAACAGACCCCTCGTTCTTCCCTCTAAATTTG
AAGTCAAGTTCAAAGTGGTTTCGGAAAATATGGAAGCCTTCTTGACCTTGGACGGTCAGGAGGGATTTCATCTAAAGAAG
GGGGACGAGGTAATCGTTAAGCGTTCAAGGTACGTTTGCAGGATGTACTCCCATCCCCGTAAGTCCTTTTTCGGGATACT
GAAAGAGAAGTTAAGATGGGGTTGA

Upstream 100 bases:

>100_bases
CCCACTACACATTCATAGTTCCCTCTTACGAGACTCAGAGGATTCAGGAGTGTCACATAACCCTCGGACACGTTCTCTGT
GAACTCGTTGAAGAGATGGT

Downstream 100 bases:

>100_bases
TAGCTTTTTAGACTTCCTGAGGTCGGAACCCTTTATAACGAGCAACTCGTTCATCTCGTAAATTTTTGAAACTACGTTTT
CTCCGAGTCTGCTTGCAAGA

Product: hypothetical protein

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MRKVLVFLKNSKKAFETFKRVERVLKDLNLSYKKFINRKELFKVLKPKDYELFLVIGGDGTFLSAARIASRFGVPLVGVN
EGRFGFLTEIKKEEIKKVLPLVLEGRAKLQERLMIDVYLRSRNRLRYLGNYLNDAVISKSSIARIIRTKVFINGEEVLEV
FGDGVILSTPTGSTAYALSAGGPIVYPESQNLLFVPICPHTLSNRPLVLPSKFEVKFKVVSENMEAFLTLDGQEGFHLKK
GDEVIVKRSRYVCRMYSHPRKSFFGILKEKLRWG

Sequences:

>Translated_274_residues
MRKVLVFLKNSKKAFETFKRVERVLKDLNLSYKKFINRKELFKVLKPKDYELFLVIGGDGTFLSAARIASRFGVPLVGVN
EGRFGFLTEIKKEEIKKVLPLVLEGRAKLQERLMIDVYLRSRNRLRYLGNYLNDAVISKSSIARIIRTKVFINGEEVLEV
FGDGVILSTPTGSTAYALSAGGPIVYPESQNLLFVPICPHTLSNRPLVLPSKFEVKFKVVSENMEAFLTLDGQEGFHLKK
GDEVIVKRSRYVCRMYSHPRKSFFGILKEKLRWG
>Mature_274_residues
MRKVLVFLKNSKKAFETFKRVERVLKDLNLSYKKFINRKELFKVLKPKDYELFLVIGGDGTFLSAARIASRFGVPLVGVN
EGRFGFLTEIKKEEIKKVLPLVLEGRAKLQERLMIDVYLRSRNRLRYLGNYLNDAVISKSSIARIIRTKVFINGEEVLEV
FGDGVILSTPTGSTAYALSAGGPIVYPESQNLLFVPICPHTLSNRPLVLPSKFEVKFKVVSENMEAFLTLDGQEGFHLKK
GDEVIVKRSRYVCRMYSHPRKSFFGILKEKLRWG

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family

Homologues:

Organism=Homo sapiens, GI55743112, Length=272, Percent_Identity=29.4117647058824, Blast_Score=100, Evalue=1e-21,
Organism=Escherichia coli, GI1788968, Length=224, Percent_Identity=33.9285714285714, Blast_Score=155, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6320794, Length=277, Percent_Identity=31.0469314079422, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6322509, Length=285, Percent_Identity=30.8771929824561, Blast_Score=126, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6325068, Length=238, Percent_Identity=32.7731092436975, Blast_Score=122, Evalue=8e-29,
Organism=Drosophila melanogaster, GI28573826, Length=245, Percent_Identity=30.2040816326531, Blast_Score=101, Evalue=6e-22,
Organism=Drosophila melanogaster, GI28573830, Length=245, Percent_Identity=30.2040816326531, Blast_Score=100, Evalue=7e-22,
Organism=Drosophila melanogaster, GI28573832, Length=245, Percent_Identity=30.2040816326531, Blast_Score=100, Evalue=7e-22,
Organism=Drosophila melanogaster, GI28573828, Length=244, Percent_Identity=30.327868852459, Blast_Score=100, Evalue=8e-22,
Organism=Drosophila melanogaster, GI161077047, Length=245, Percent_Identity=30.2040816326531, Blast_Score=100, Evalue=9e-22,
Organism=Drosophila melanogaster, GI20129957, Length=269, Percent_Identity=29.7397769516729, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24653424, Length=270, Percent_Identity=29.6296296296296, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24653422, Length=270, Percent_Identity=29.6296296296296, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI281363321, Length=270, Percent_Identity=29.6296296296296, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI281363323, Length=270, Percent_Identity=29.6296296296296, Blast_Score=93, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPNK_AQUAE (O67055)

Other databases:

- EMBL:   AE000657
- PIR:   D70378
- RefSeq:   NP_213617.1
- ProteinModelPortal:   O67055
- SMR:   O67055
- GeneID:   1193597
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_909
- NMPDR:   fig|224324.1.peg.632
- HOGENOM:   HBG713904
- OMA:   LNEMVLH
- PhylomeDB:   O67055
- ProtClustDB:   CLSK2299545
- BioCyc:   AAEO224324:AQ_909-MONOMER
- BRENDA:   2.7.1.23
- GO:   GO:0005737
- HAMAP:   MF_00361
- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504
- Gene3D:   G3DSA:2.60.200.30
- Gene3D:   G3DSA:3.40.50.10330
- PANTHER:   PTHR20275

Pfam domain/function: PF01513 NAD_kinase; SSF111331 ATP-NAD_kinase_PpnK-typ

EC number: =2.7.1.23

Molecular weight: Translated: 31327; Mature: 31327

Theoretical pI: Translated: 10.60; Mature: 10.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKVLVFLKNSKKAFETFKRVERVLKDLNLSYKKFINRKELFKVLKPKDYELFLVIGGDG
CCEEEEEEECCHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHCCCCCEEEEEECCCC
TFLSAARIASRFGVPLVGVNEGRFGFLTEIKKEEIKKVLPLVLEGRAKLQERLMIDVYLR
CHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHEEEEEEC
SRNRLRYLGNYLNDAVISKSSIARIIRTKVFINGEEVLEVFGDGVILSTPTGSTAYALSA
CCCHHHHHHHHHHHHHHCHHHHHHHHHHHEEECHHHHHHHHCCCEEEECCCCCCEEEEEC
GGPIVYPESQNLLFVPICPHTLSNRPLVLPSKFEVKFKVVSENMEAFLTLDGQEGFHLKK
CCCEEEECCCCEEEEEECCCCCCCCCEECCCCCEEEEEEEECCCEEEEEECCCCCCEECC
GDEVIVKRSRYVCRMYSHPRKSFFGILKEKLRWG
CCCEEEHHHHHHHHHHCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRKVLVFLKNSKKAFETFKRVERVLKDLNLSYKKFINRKELFKVLKPKDYELFLVIGGDG
CCEEEEEEECCHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHCCCCCEEEEEECCCC
TFLSAARIASRFGVPLVGVNEGRFGFLTEIKKEEIKKVLPLVLEGRAKLQERLMIDVYLR
CHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHEEEEEEC
SRNRLRYLGNYLNDAVISKSSIARIIRTKVFINGEEVLEVFGDGVILSTPTGSTAYALSA
CCCHHHHHHHHHHHHHHCHHHHHHHHHHHEEECHHHHHHHHCCCEEEECCCCCCEEEEEC
GGPIVYPESQNLLFVPICPHTLSNRPLVLPSKFEVKFKVVSENMEAFLTLDGQEGFHLKK
CCCEEEECCCCEEEEEECCCCCCCCCEECCCCCEEEEEEEECCCEEEEEECCCCCCEECC
GDEVIVKRSRYVCRMYSHPRKSFFGILKEKLRWG
CCCEEEHHHHHHHHHHCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320