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The map label for this gene is purN [H]

Identifier: 15606207

GI number: 15606207

Start: 592596

End: 593246

Strand: Direct

Name: purN [H]

Synonym: aq_857

Alternate gene names: 15606207

Gene position: 592596-593246 (Clockwise)

Preceding gene: 15606199

Following gene: 15606214

Centisome position: 38.2

GC content: 43.16

Gene sequence:

>651_bases
ATGCTAAAAATAGGAGTACTGGTTTCGGGAAGAGGAAGCAACCTTCAAGCGATAATAGACGCCATAGAAAGCGGAAAGGT
AAACGCGAGTATAGAACTCGTGATTTCCGACAACCCTAAAGCCTACGCCATAGAAAGGTGTAAAAAACACAACGTAGAGT
GTAAAGTAATTCAGAGAAAGGAATTCCCTTCAAAAAAAGAGTTTGAAGAGAGAATGGCTCTTGAGCTGAAGAAAAAGGGT
GTTGAACTTGTAGTGCTTGCGGGTTTTATGAGAATACTCTCTCACAACTTTTTAAAGTACTTTCCGAATAAGGTTATAAA
CATACACCCTTCCCTAATTCCCGCCTTTCAGGGGCTGCACGCTCAAAAACAAGCAGTTGAGTTCGGTGTGAAGTTTTCCG
GTTGCACCGTTCACATAGTGGATGAGAGCGTTGATGCTGGACCCGTAATAGTTCAGGCTGTAGTTCCGGTGCTCCCCGAA
GATGATGAAAACACCTTGGCGGACAGAATTTTAAAGTGGGAACACAAAATACTCCCTCAAACTGTCCAGTGGTTTGCTCA
GGACAGGATAATTATCGACGGAAGGAAGGTAATAGTAAAAGACGCAACCTACGGAACGCTTCCAGTAAATCCCGCTCTTG
AGATTTTTTAA

Upstream 100 bases:

>100_bases
TTTCCCCCACCCTCTTTACGGTTCTCAGGAGCGGGTTTGTTATCTCTGAGAAGTCAACGGGAACTAAGAACTTCATAGTA
AGCATTTTAAAATAACAAGA

Downstream 100 bases:

>100_bases
GTTTTTTGTAGAGTCCTTTCGAGGGCTTCCTTTCCTTTATCCGTTATGTAGTACTTTCCTTCTTTTTCTTCAATTAAACC
CGCGTTTTTGAGTTCCCTCA

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MLKIGVLVSGRGSNLQAIIDAIESGKVNASIELVISDNPKAYAIERCKKHNVECKVIQRKEFPSKKEFEERMALELKKKG
VELVVLAGFMRILSHNFLKYFPNKVINIHPSLIPAFQGLHAQKQAVEFGVKFSGCTVHIVDESVDAGPVIVQAVVPVLPE
DDENTLADRILKWEHKILPQTVQWFAQDRIIIDGRKVIVKDATYGTLPVNPALEIF

Sequences:

>Translated_216_residues
MLKIGVLVSGRGSNLQAIIDAIESGKVNASIELVISDNPKAYAIERCKKHNVECKVIQRKEFPSKKEFEERMALELKKKG
VELVVLAGFMRILSHNFLKYFPNKVINIHPSLIPAFQGLHAQKQAVEFGVKFSGCTVHIVDESVDAGPVIVQAVVPVLPE
DDENTLADRILKWEHKILPQTVQWFAQDRIIIDGRKVIVKDATYGTLPVNPALEIF
>Mature_216_residues
MLKIGVLVSGRGSNLQAIIDAIESGKVNASIELVISDNPKAYAIERCKKHNVECKVIQRKEFPSKKEFEERMALELKKKG
VELVVLAGFMRILSHNFLKYFPNKVINIHPSLIPAFQGLHAQKQAVEFGVKFSGCTVHIVDESVDAGPVIVQAVVPVLPE
DDENTLADRILKWEHKILPQTVQWFAQDRIIIDGRKVIVKDATYGTLPVNPALEIF

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=188, Percent_Identity=41.4893617021277, Blast_Score=158, Evalue=3e-39,
Organism=Homo sapiens, GI209869995, Length=188, Percent_Identity=41.4893617021277, Blast_Score=158, Evalue=3e-39,
Organism=Homo sapiens, GI209869993, Length=188, Percent_Identity=41.4893617021277, Blast_Score=158, Evalue=3e-39,
Organism=Escherichia coli, GI1788846, Length=189, Percent_Identity=39.6825396825397, Blast_Score=163, Evalue=6e-42,
Organism=Escherichia coli, GI1787483, Length=201, Percent_Identity=28.3582089552239, Blast_Score=91, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17567511, Length=181, Percent_Identity=37.5690607734807, Blast_Score=130, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6320616, Length=201, Percent_Identity=29.8507462686567, Blast_Score=80, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24582400, Length=192, Percent_Identity=44.2708333333333, Blast_Score=174, Evalue=3e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 24147; Mature: 24147

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKIGVLVSGRGSNLQAIIDAIESGKVNASIELVISDNPKAYAIERCKKHNVECKVIQRK
CEEEEEEEECCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHCCCEEEEEECC
EFPSKKEFEERMALELKKKGVELVVLAGFMRILSHNFLKYFPNKVINIHPSLIPAFQGLH
CCCCHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHCCH
AQKQAVEFGVKFSGCTVHIVDESVDAGPVIVQAVVPVLPEDDENTLADRILKWEHKILPQ
HHHHHHHHCCEECCEEEEEECCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
TVQWFAQDRIIIDGRKVIVKDATYGTLPVNPALEIF
HHHHHHCCCEEECCCEEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLKIGVLVSGRGSNLQAIIDAIESGKVNASIELVISDNPKAYAIERCKKHNVECKVIQRK
CEEEEEEEECCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHCCCEEEEEECC
EFPSKKEFEERMALELKKKGVELVVLAGFMRILSHNFLKYFPNKVINIHPSLIPAFQGLH
CCCCHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHCCH
AQKQAVEFGVKFSGCTVHIVDESVDAGPVIVQAVVPVLPEDDENTLADRILKWEHKILPQ
HHHHHHHHCCEECCEEEEEECCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
TVQWFAQDRIIIDGRKVIVKDATYGTLPVNPALEIF
HHHHHHCCCEEECCCEEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]