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The map label for this gene is pilT [H]

Identifier: 15606134

GI number: 15606134

Start: 516321

End: 517421

Strand: Direct

Name: pilT [H]

Synonym: aq_745

Alternate gene names: 15606134

Gene position: 516321-517421 (Clockwise)

Preceding gene: 15606132

Following gene: 15606135

Centisome position: 33.28

GC content: 43.69

Gene sequence:

>1101_bases
ATGTTTGAAAAACAAGAAGTAGAACAGAAAAAAGAATTAAAGATCCTTGAAATAATAAAGGAAGCAATAGAACTTGGGGC
AAGCGATATACACCTCACCGCAGGCGCACCACCCGCGGTCAGGATTGACGGTTACATAAAGTTTTTGAAAGACTTTCCGA
GACTGACTCCGGAGGATACACAAAAGCTGGCTTATTCAGTTATGTCAGAAAAACACAGACAGAAGCTGGAAGAGAACGGA
CAGGTGGACTTTTCCTTCGGAGTGAGAGGAGTTGGAAGGTTCAGGGCTAACGTTTTTTACCAGAGGGGTTCGGTAGCAGC
AGCGTTGAGATCCCTTCCCGCTGAAATTCCTGAATTTAAAAAACTGGGACTTCCCGATAAAGTTCTTGAACTCTGTCATA
GAAAGATGGGGTTAATTCTCGTTACAGGTCCTACGGGGTCGGGTAAGTCTACAACTATAGCTTCTATGATTGATTACATA
AACCAAACTAAGTCCTATCACATAATAACGATAGAAGATCCCATTGAGTACGTTTTTAAGCACAAAAAGTCTATAGTGAA
CCAAAGGGAAGTGGGTGAAGATACCAAAAGCTTTGCCGACGCTCTCAGGGCAGCTCTCAGAGAAGACCCGGACGTCATAT
TCGTCGGTGAGATGAGAGATCTGGAAACCGTTGAAACCGCACTCAGGGCTGCGGAAACGGGACACCTTGTTTTCGGAACA
CTTCACACCAACACGGCTATAGACACCATACACAGGATAGTGGACATATTCCCCCTGAATCAACAGGAACAAGTAAGAAT
AGTGCTTTCTTTTATCTTGCAAGGAATTATTTCTCAAAGGCTTCTACCTAAAATTGGAGGCGGCAGAGTTCTTGCATACG
AATTACTTATTCCCAACACAGCTATAAGGAATCTAATAAGGGAAAACAAACTCCAGCAGGTTTACTCCCTTATGCAAAGC
GGTCAAGCCGAAACGGGAATGCAGACCATGAACCAGACCCTTTACAAGCTTTATAAACAAGGTTTAATAACCTTGGAAGA
TGCTATGGAAGCCTCTCCCGATCCAAAAGAGCTTGAAAGAATGATTAGGGGAGGCAGGTAG

Upstream 100 bases:

>100_bases
CTCTGTGCCACCTTCCACGCTATTGCGTGTTCTCTTCCTCCGTTTCCTACTACAAGTACCTTCACGTTAAACTTATTATA
TAAGTGGTAAGGAGTTTACG

Downstream 100 bases:

>100_bases
TGCCTCTTTTCAAGTACAAGGCCGTAACTCCTGAAGGAAGAGTAGTTGAGGGGCAGGGGGAGTTCCCTTCAGTTTTAGAA
CTTTACGAAAGGCTCAGTGC

Product: twitching motility protein PilT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MFEKQEVEQKKELKILEIIKEAIELGASDIHLTAGAPPAVRIDGYIKFLKDFPRLTPEDTQKLAYSVMSEKHRQKLEENG
QVDFSFGVRGVGRFRANVFYQRGSVAAALRSLPAEIPEFKKLGLPDKVLELCHRKMGLILVTGPTGSGKSTTIASMIDYI
NQTKSYHIITIEDPIEYVFKHKKSIVNQREVGEDTKSFADALRAALREDPDVIFVGEMRDLETVETALRAAETGHLVFGT
LHTNTAIDTIHRIVDIFPLNQQEQVRIVLSFILQGIISQRLLPKIGGGRVLAYELLIPNTAIRNLIRENKLQQVYSLMQS
GQAETGMQTMNQTLYKLYKQGLITLEDAMEASPDPKELERMIRGGR

Sequences:

>Translated_366_residues
MFEKQEVEQKKELKILEIIKEAIELGASDIHLTAGAPPAVRIDGYIKFLKDFPRLTPEDTQKLAYSVMSEKHRQKLEENG
QVDFSFGVRGVGRFRANVFYQRGSVAAALRSLPAEIPEFKKLGLPDKVLELCHRKMGLILVTGPTGSGKSTTIASMIDYI
NQTKSYHIITIEDPIEYVFKHKKSIVNQREVGEDTKSFADALRAALREDPDVIFVGEMRDLETVETALRAAETGHLVFGT
LHTNTAIDTIHRIVDIFPLNQQEQVRIVLSFILQGIISQRLLPKIGGGRVLAYELLIPNTAIRNLIRENKLQQVYSLMQS
GQAETGMQTMNQTLYKLYKQGLITLEDAMEASPDPKELERMIRGGR
>Mature_366_residues
MFEKQEVEQKKELKILEIIKEAIELGASDIHLTAGAPPAVRIDGYIKFLKDFPRLTPEDTQKLAYSVMSEKHRQKLEENG
QVDFSFGVRGVGRFRANVFYQRGSVAAALRSLPAEIPEFKKLGLPDKVLELCHRKMGLILVTGPTGSGKSTTIASMIDYI
NQTKSYHIITIEDPIEYVFKHKKSIVNQREVGEDTKSFADALRAALREDPDVIFVGEMRDLETVETALRAAETGHLVFGT
LHTNTAIDTIHRIVDIFPLNQQEQVRIVLSFILQGIISQRLLPKIGGGRVLAYELLIPNTAIRNLIRENKLQQVYSLMQS
GQAETGMQTMNQTLYKLYKQGLITLEDAMEASPDPKELERMIRGGR

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=317, Percent_Identity=44.1640378548896, Blast_Score=265, Evalue=4e-72,
Organism=Escherichia coli, GI1786296, Length=395, Percent_Identity=31.3924050632911, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1789723, Length=287, Percent_Identity=33.7979094076655, Blast_Score=128, Evalue=5e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 41206; Mature: 41206

Theoretical pI: Translated: 7.81; Mature: 7.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEKQEVEQKKELKILEIIKEAIELGASDIHLTAGAPPAVRIDGYIKFLKDFPRLTPEDT
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEHHHHHHHHHHCCCCCCHHH
QKLAYSVMSEKHRQKLEENGQVDFSFGVRGVGRFRANVFYQRGSVAAALRSLPAEIPEFK
HHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHH
KLGLPDKVLELCHRKMGLILVTGPTGSGKSTTIASMIDYINQTKSYHIITIEDPIEYVFK
HCCCCHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHH
HKKSIVNQREVGEDTKSFADALRAALREDPDVIFVGEMRDLETVETALRAAETGHLVFGT
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCCEEEEE
LHTNTAIDTIHRIVDIFPLNQQEQVRIVLSFILQGIISQRLLPKIGGGRVLAYELLIPNT
ECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCH
AIRNLIRENKLQQVYSLMQSGQAETGMQTMNQTLYKLYKQGLITLEDAMEASPDPKELER
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEHHHHHCCCCCHHHHHH
MIRGGR
HHHCCC
>Mature Secondary Structure
MFEKQEVEQKKELKILEIIKEAIELGASDIHLTAGAPPAVRIDGYIKFLKDFPRLTPEDT
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEHHHHHHHHHHCCCCCCHHH
QKLAYSVMSEKHRQKLEENGQVDFSFGVRGVGRFRANVFYQRGSVAAALRSLPAEIPEFK
HHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHH
KLGLPDKVLELCHRKMGLILVTGPTGSGKSTTIASMIDYINQTKSYHIITIEDPIEYVFK
HCCCCHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHH
HKKSIVNQREVGEDTKSFADALRAALREDPDVIFVGEMRDLETVETALRAAETGHLVFGT
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCCEEEEE
LHTNTAIDTIHRIVDIFPLNQQEQVRIVLSFILQGIISQRLLPKIGGGRVLAYELLIPNT
ECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCH
AIRNLIRENKLQQVYSLMQSGQAETGMQTMNQTLYKLYKQGLITLEDAMEASPDPKELER
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEHHHHHCCCCCHHHHHH
MIRGGR
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]