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The map label for this gene is ddlA

Identifier: 15605989

GI number: 15605989

Start: 354044

End: 354919

Strand: Direct

Name: ddlA

Synonym: aq_521

Alternate gene names: 15605989

Gene position: 354044-354919 (Clockwise)

Preceding gene: 15605988

Following gene: 15605990

Centisome position: 22.82

GC content: 40.53

Gene sequence:

>876_bases
TTGAGAGTAGTGGTTCTGATGGGTGGAAGGTCCTCGGAGCGTGAAATATCCTTGAAAACGGGAAAGGCCGTTGCAAAGGC
ATTAAGAGAACTGGGGCACGAAGTTTACGAGCTTGACCTTGATAAGGAACTTCCCTGTAAACTCCTCGAAATAAAGCCCG
ATAAAGTATTTATAGCCCTTCACGGAAGGTACGGAGAGGACGGAACCGTGCAGGGATTGCTGGAGATTTTAGACATACCT
TACACGGGTTCGGATACGATAGCTTCCGCTGTATCTATAGACAAGGACTTTACAAAGAGAATAGTAAAAAGCCTTGGTAT
AAACACACCTGACTGGGAAACGTTCATTTCGGAGGGAGATGTTCTAAATACAGAATGGAATAAATTTCCCGCGGTGGTAA
AGCCGGTAAGAGAAGGATCAAGCGTTGGGCTGAAAATAGTGGAAAGTCTTGAAGAATTAAAAGAGTACGCCTTAGACCTC
CTTAAAAAAACCGAAAGGGTTATGGTTGAGGAATTCGTGGAAGGGAGGGACATGACTGTAGGGATACTGAAAGGGGAAGC
CCTTCCCGTTGTGGAAATAATACCTAAAAAAGGAGTTTACGACTACGAGTGTAAGTACACAAAAGGTATGAGCGAGTACA
GAATTTTAAAAGATGAAAAATTATCTAAAAAACTCCAGGAAATCTCCCTCAAAATTTCTAAATTTCTCTCTTTAAAGGAC
TTTGCAAGAATTGACTTCAGAGTGACAAAAGAAGGTAAAATATTTTTCTTAGAGGTAAATACCATACCAGGGATGACTGA
ACTCAGTCTCCTTCCTATGGCAGCGAAAGAAAAAGGTATGGACTTTAAAAAATTAATATCTATAATTATCACTTAA

Upstream 100 bases:

>100_bases
GGAGGTGGAACTTTTTCGGAAGTGGTAGATTTAATAAATATTGCAAAGGAAAGGGTTTACGAAAACTTCGGTATAGTATT
GGAGGAGGAGGTAAAGCTGA

Downstream 100 bases:

>100_bases
GGGGGGAATATTGAGGGGTATACTTGGACTAGTATTCGGAAGTGTAATCCTCATATATTTAATCTCTCTGTTCCTTATGA
AGCATCCCTTTTTTCAGATA

Product: D-alanine:D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MRVVVLMGGRSSEREISLKTGKAVAKALRELGHEVYELDLDKELPCKLLEIKPDKVFIALHGRYGEDGTVQGLLEILDIP
YTGSDTIASAVSIDKDFTKRIVKSLGINTPDWETFISEGDVLNTEWNKFPAVVKPVREGSSVGLKIVESLEELKEYALDL
LKKTERVMVEEFVEGRDMTVGILKGEALPVVEIIPKKGVYDYECKYTKGMSEYRILKDEKLSKKLQEISLKISKFLSLKD
FARIDFRVTKEGKIFFLEVNTIPGMTELSLLPMAAKEKGMDFKKLISIIIT

Sequences:

>Translated_291_residues
MRVVVLMGGRSSEREISLKTGKAVAKALRELGHEVYELDLDKELPCKLLEIKPDKVFIALHGRYGEDGTVQGLLEILDIP
YTGSDTIASAVSIDKDFTKRIVKSLGINTPDWETFISEGDVLNTEWNKFPAVVKPVREGSSVGLKIVESLEELKEYALDL
LKKTERVMVEEFVEGRDMTVGILKGEALPVVEIIPKKGVYDYECKYTKGMSEYRILKDEKLSKKLQEISLKISKFLSLKD
FARIDFRVTKEGKIFFLEVNTIPGMTELSLLPMAAKEKGMDFKKLISIIIT
>Mature_291_residues
MRVVVLMGGRSSEREISLKTGKAVAKALRELGHEVYELDLDKELPCKLLEIKPDKVFIALHGRYGEDGTVQGLLEILDIP
YTGSDTIASAVSIDKDFTKRIVKSLGINTPDWETFISEGDVLNTEWNKFPAVVKPVREGSSVGLKIVESLEELKEYALDL
LKKTERVMVEEFVEGRDMTVGILKGEALPVVEIIPKKGVYDYECKYTKGMSEYRILKDEKLSKKLQEISLKISKFLSLKD
FARIDFRVTKEGKIFFLEVNTIPGMTELSLLPMAAKEKGMDFKKLISIIIT

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=299, Percent_Identity=39.1304347826087, Blast_Score=206, Evalue=1e-54,
Organism=Escherichia coli, GI1786579, Length=346, Percent_Identity=26.878612716763, Blast_Score=134, Evalue=9e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_AQUAE (O66806)

Other databases:

- EMBL:   AE000657
- PIR:   B70347
- RefSeq:   NP_213366.1
- ProteinModelPortal:   O66806
- SMR:   O66806
- GeneID:   1193224
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_521
- NMPDR:   fig|224324.1.peg.381
- HOGENOM:   HBG427092
- OMA:   PLSIVPE
- PhylomeDB:   O66806
- ProtClustDB:   CLSK2299419
- BioCyc:   AAEO224324:AQ_521-MONOMER
- BRENDA:   6.3.2.4
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 32798; Mature: 32798

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVVVLMGGRSSEREISLKTGKAVAKALRELGHEVYELDLDKELPCKLLEIKPDKVFIAL
CEEEEEECCCCCCCEEEEHHHHHHHHHHHHHCHHHEEECCCCCCCCEEEEECCCEEEEEE
HGRYGEDGTVQGLLEILDIPYTGSDTIASAVSIDKDFTKRIVKSLGINTPDWETFISEGD
ECCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHCCCC
VLNTEWNKFPAVVKPVREGSSVGLKIVESLEELKEYALDLLKKTERVMVEEFVEGRDMTV
EECCCCHHCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
GILKGEALPVVEIIPKKGVYDYECKYTKGMSEYRILKDEKLSKKLQEISLKISKFLSLKD
EEECCCCCCEEEECCCCCCCCEECHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FARIDFRVTKEGKIFFLEVNTIPGMTELSLLPMAAKEKGMDFKKLISIIIT
HHEEEEEEECCCCEEEEEECCCCCCHHHHHCCCHHHHCCCCHHHHHHHHCC
>Mature Secondary Structure
MRVVVLMGGRSSEREISLKTGKAVAKALRELGHEVYELDLDKELPCKLLEIKPDKVFIAL
CEEEEEECCCCCCCEEEEHHHHHHHHHHHHHCHHHEEECCCCCCCCEEEEECCCEEEEEE
HGRYGEDGTVQGLLEILDIPYTGSDTIASAVSIDKDFTKRIVKSLGINTPDWETFISEGD
ECCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHCCCC
VLNTEWNKFPAVVKPVREGSSVGLKIVESLEELKEYALDLLKKTERVMVEEFVEGRDMTV
EECCCCHHCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
GILKGEALPVVEIIPKKGVYDYECKYTKGMSEYRILKDEKLSKKLQEISLKISKFLSLKD
EEECCCCCCEEEECCCCCCCCEECHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FARIDFRVTKEGKIFFLEVNTIPGMTELSLLPMAAKEKGMDFKKLISIIIT
HHEEEEEEECCCCEEEEEECCCCCCHHHHHCCCHHHHCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320