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The map label for this gene is gatB

Identifier: 15605949

GI number: 15605949

Start: 312402

End: 313838

Strand: Direct

Name: gatB

Synonym: aq_461

Alternate gene names: 15605949

Gene position: 312402-313838 (Clockwise)

Preceding gene: 15605948

Following gene: 15605950

Centisome position: 20.14

GC content: 45.23

Gene sequence:

>1437_bases
ATGAACGAAAAGTACGAGGCTGTAATCGGTCTTGAAATACACGTGCAGATGGACACAAAGACTAAGATGTTCTGTGGTTG
TAAAGTGGAATTCGGAGCGGAGCCAAACACAAACGTTTGCCCCGTATGCCTCGGAATGCCCGGAGCACTTCCCATTGTCA
ACAAAAGGGCGGTTGAGTACGCGATAAGGGCTTCTCTGGCACTGAACTGCGAGGTTCACGAAGAGTCCGTATTTGCGAGA
AAACACTACTTTTACCCGGACCTCCCTAAGGGATACCAGATATCCCAGTACGAAAAGCCCCTCGCCACGAACGGATGGGT
GGAGTTAAACCTTCCAAACGGTGAAAAGAAAAAGGTAAGGATAAGGAGACTCCACATAGAAGAGGACGCGGGCAAGAACA
TACACGAGGGTGATAAAACGCTCGTTGACCTAAACAGGGCTGGAACTCCCCTTATGGAAATAGTAACAGAGCCCGACATA
AGAACACCCGAAGAGGCGAGACTCTTTTTAGAAAAACTCAGGAACATAATGAGGTACGCGGGTGTTTCTAAAGCGGATAT
GGAAAAGGGACAACTCAGGTGTGACATAAACGTTTCCATCAGACCGAAGGGCAGTAAAGAGTTCGGAACGAGGGTTGAGA
TAAAGAACGTAAACTCCTTCAGGTTCGTCCAGAAAGCCCTTGAGTACGAGATAGAAAGACAAATTAATGTGGTTGAGGAG
GGCGGAGAAGTCGTCCAGGAAACGAGAACCTTTGACCCTCAAACGGGCAAAACCTACCCCATGAGGACAAAAGAAGAGGC
GGAAGATTACAGGTACTTCCCGGACCCGGACCTCGTTCCCCTGAAGGTAAAAAAGGAGTGGATAGAAGAAATAAAGAAGA
ACATGCCCGAACTCCCGGACCAAAGGTTTGAAAGATTAATAAAAGAGTACGGACTTTCTGAATACGAGGCAGGAATACTC
GTAAACCACAAGGAAGTGGGAGACTTCTTTGAAGAAGCGGTAAGACATTTCAAGGAACCCAAAGGGATAGTAAACTGGCT
CATAAACGACCTTCTAGGACTCTTAAGGGATAAAGGCATCTCAATAGAAGAGAGTCCCGTAAAGCCCGAGCACTTAGCTG
AACTCGTAAAACTCATAAAGGAAAAGGTTATATCCACGAAGATAGGAAAAGAAGTTATAAAGGAAATGGTTGAAACCGGA
AAAACGCCTTCCCAGATAGTTGAAGAAAAGGGACTAAAGCAGATAACCGACGAAAACCAAATAAAGGAGCTCGTAAAGAA
GATATTTGAAAAACACCCCAAGGAAGTGGAAAGATTAAAACAGGGTGAGGAAAAACTTATTGGCTTTTTCGTAGGACAGG
TAATGAGGGAAACAAGAGGAAAGGCAAACCCCCAAGTCGTGAATAAAGTCATAAGGGAACTGGTAAAAGAAGTGTAA

Upstream 100 bases:

>100_bases
TTGAACTCGTGGAGTGGATAAAAAAAGAGGGGATAACCTACCCCTATTACGACCAAGTAAAGGAGAGACTACTTCAAAAA
CTCAAGTAGAATTTATTCTT

Downstream 100 bases:

>100_bases
AATAAAATTTAAATGAACAGGCAACCTATGTTCTTCATACTTATTGTCTTACTCTTTACCGTTTTCTCCTTAAAAGAGTT
TATTCCTAATACCTTCTGCA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MNEKYEAVIGLEIHVQMDTKTKMFCGCKVEFGAEPNTNVCPVCLGMPGALPIVNKRAVEYAIRASLALNCEVHEESVFAR
KHYFYPDLPKGYQISQYEKPLATNGWVELNLPNGEKKKVRIRRLHIEEDAGKNIHEGDKTLVDLNRAGTPLMEIVTEPDI
RTPEEARLFLEKLRNIMRYAGVSKADMEKGQLRCDINVSIRPKGSKEFGTRVEIKNVNSFRFVQKALEYEIERQINVVEE
GGEVVQETRTFDPQTGKTYPMRTKEEAEDYRYFPDPDLVPLKVKKEWIEEIKKNMPELPDQRFERLIKEYGLSEYEAGIL
VNHKEVGDFFEEAVRHFKEPKGIVNWLINDLLGLLRDKGISIEESPVKPEHLAELVKLIKEKVISTKIGKEVIKEMVETG
KTPSQIVEEKGLKQITDENQIKELVKKIFEKHPKEVERLKQGEEKLIGFFVGQVMRETRGKANPQVVNKVIRELVKEV

Sequences:

>Translated_478_residues
MNEKYEAVIGLEIHVQMDTKTKMFCGCKVEFGAEPNTNVCPVCLGMPGALPIVNKRAVEYAIRASLALNCEVHEESVFAR
KHYFYPDLPKGYQISQYEKPLATNGWVELNLPNGEKKKVRIRRLHIEEDAGKNIHEGDKTLVDLNRAGTPLMEIVTEPDI
RTPEEARLFLEKLRNIMRYAGVSKADMEKGQLRCDINVSIRPKGSKEFGTRVEIKNVNSFRFVQKALEYEIERQINVVEE
GGEVVQETRTFDPQTGKTYPMRTKEEAEDYRYFPDPDLVPLKVKKEWIEEIKKNMPELPDQRFERLIKEYGLSEYEAGIL
VNHKEVGDFFEEAVRHFKEPKGIVNWLINDLLGLLRDKGISIEESPVKPEHLAELVKLIKEKVISTKIGKEVIKEMVETG
KTPSQIVEEKGLKQITDENQIKELVKKIFEKHPKEVERLKQGEEKLIGFFVGQVMRETRGKANPQVVNKVIRELVKEV
>Mature_478_residues
MNEKYEAVIGLEIHVQMDTKTKMFCGCKVEFGAEPNTNVCPVCLGMPGALPIVNKRAVEYAIRASLALNCEVHEESVFAR
KHYFYPDLPKGYQISQYEKPLATNGWVELNLPNGEKKKVRIRRLHIEEDAGKNIHEGDKTLVDLNRAGTPLMEIVTEPDI
RTPEEARLFLEKLRNIMRYAGVSKADMEKGQLRCDINVSIRPKGSKEFGTRVEIKNVNSFRFVQKALEYEIERQINVVEE
GGEVVQETRTFDPQTGKTYPMRTKEEAEDYRYFPDPDLVPLKVKKEWIEEIKKNMPELPDQRFERLIKEYGLSEYEAGIL
VNHKEVGDFFEEAVRHFKEPKGIVNWLINDLLGLLRDKGISIEESPVKPEHLAELVKLIKEKVISTKIGKEVIKEMVETG
KTPSQIVEEKGLKQITDENQIKELVKKIFEKHPKEVERLKQGEEKLIGFFVGQVMRETRGKANPQVVNKVIRELVKEV

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=492, Percent_Identity=39.4308943089431, Blast_Score=376, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI115534094, Length=463, Percent_Identity=33.4773218142549, Blast_Score=238, Evalue=4e-63,
Organism=Saccharomyces cerevisiae, GI6319391, Length=517, Percent_Identity=31.5280464216634, Blast_Score=246, Evalue=6e-66,
Organism=Drosophila melanogaster, GI45550808, Length=492, Percent_Identity=35.1626016260163, Blast_Score=315, Evalue=6e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_AQUAE (O66766)

Other databases:

- EMBL:   AE000657
- PIR:   B70342
- RefSeq:   NP_213326.1
- PDB:   3H0L
- PDB:   3H0M
- PDB:   3H0R
- PDBsum:   3H0L
- PDBsum:   3H0M
- PDBsum:   3H0R
- ProteinModelPortal:   O66766
- SMR:   O66766
- GeneID:   1193092
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_461
- NMPDR:   fig|224324.1.peg.341
- HOGENOM:   HBG395951
- OMA:   RIHGATH
- PhylomeDB:   O66766
- ProtClustDB:   PRK05477
- BioCyc:   AAEO224324:AQ_461-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 55041; Mature: 55041

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEKYEAVIGLEIHVQMDTKTKMFCGCKVEFGAEPNTNVCPVCLGMPGALPIVNKRAVEY
CCCCEEEEEEEEEEEEECCCCEEEEEEEEEECCCCCCCCCEEEECCCCCCCHHHHHHHHH
AIRASLALNCEVHEESVFARKHYFYPDLPKGYQISQYEKPLATNGWVELNLPNGEKKKVR
HHHHHHEEEEEEHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCEEEEECCCCCCHHEE
IRRLHIEEDAGKNIHEGDKTLVDLNRAGTPLMEIVTEPDIRTPEEARLFLEKLRNIMRYA
EEEEEEHHCCCCCCCCCCCCEEECCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHC
GVSKADMEKGQLRCDINVSIRPKGSKEFGTRVEIKNVNSFRFVQKALEYEIERQINVVEE
CCCHHHCCCCCEEEEEEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
GGEVVQETRTFDPQTGKTYPMRTKEEAEDYRYFPDPDLVPLKVKKEWIEEIKKNMPELPD
HHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCH
QRFERLIKEYGLSEYEAGILVNHKEVGDFFEEAVRHFKEPKGIVNWLINDLLGLLRDKGI
HHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC
SIEESPVKPEHLAELVKLIKEKVISTKIGKEVIKEMVETGKTPSQIVEEKGLKQITDENQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHH
IKELVKKIFEKHPKEVERLKQGEEKLIGFFVGQVMRETRGKANPQVVNKVIRELVKEV
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHC
>Mature Secondary Structure
MNEKYEAVIGLEIHVQMDTKTKMFCGCKVEFGAEPNTNVCPVCLGMPGALPIVNKRAVEY
CCCCEEEEEEEEEEEEECCCCEEEEEEEEEECCCCCCCCCEEEECCCCCCCHHHHHHHHH
AIRASLALNCEVHEESVFARKHYFYPDLPKGYQISQYEKPLATNGWVELNLPNGEKKKVR
HHHHHHEEEEEEHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCEEEEECCCCCCHHEE
IRRLHIEEDAGKNIHEGDKTLVDLNRAGTPLMEIVTEPDIRTPEEARLFLEKLRNIMRYA
EEEEEEHHCCCCCCCCCCCCEEECCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHC
GVSKADMEKGQLRCDINVSIRPKGSKEFGTRVEIKNVNSFRFVQKALEYEIERQINVVEE
CCCHHHCCCCCEEEEEEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
GGEVVQETRTFDPQTGKTYPMRTKEEAEDYRYFPDPDLVPLKVKKEWIEEIKKNMPELPD
HHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCH
QRFERLIKEYGLSEYEAGILVNHKEVGDFFEEAVRHFKEPKGIVNWLINDLLGLLRDKGI
HHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC
SIEESPVKPEHLAELVKLIKEKVISTKIGKEVIKEMVETGKTPSQIVEEKGLKQITDENQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHH
IKELVKKIFEKHPKEVERLKQGEEKLIGFFVGQVMRETRGKANPQVVNKVIRELVKEV
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320