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The map label for this gene is rlmN

Identifier: 15605915

GI number: 15605915

Start: 285507

End: 286553

Strand: Direct

Name: rlmN

Synonym: aq_416

Alternate gene names: 15605915

Gene position: 285507-286553 (Clockwise)

Preceding gene: 15605914

Following gene: 15605916

Centisome position: 18.4

GC content: 45.85

Gene sequence:

>1047_bases
ATGGAATTTGTAACGAATTACACGCTAGAAGAGCTTAAAAAACGCTTCACCGAACTGGGACTCGAACCTTACAGGGCAAA
GCAGGTATTCAGGTGGGTATACAAGAAATTCGTAACGGACTTTGAAAAAATGACGGATTTAGGGAAAAAACACAGGGAGC
TTCTAAAGGAGCATTTTGCCTTCCACCCCTTGGAAAAGCTTGACAGGGTGGAGGCTCCCGACGCTGTAAAGTACCTATTC
AAAACAAAAGACGGACACATACTTGAGACGGTTTTGATAAAGGAGAGGGATCACTACACCCTTTGCGTTTCCTCTCAAAT
CGGTTGTGCGGTTGGATGTACCTTCTGTGCCACGGCTCTTGACGGCTTGAAGAGAAACTTGAGCACCGCGGAAATTATAG
ATCAGTACCTTCAAGTTCAGCAGGATCTGGGAGAGGAAAAAATAAGGAACGTGGTTTTCATGGGAATGGGAGAGCCCCTC
GCTAATTACGAGAACGTGAGAAAGGCTGTGGAGATTATGGTCTCTCCCGAGGGACTCGACCTTTCAAAGAGGAGGATTAC
CATATCCACGAGCGGTATAGTGGCTCAAATAAAGAGAATGGCTCAAGATCCCGTAATGAAGGAAGTTAACCTCGCCGTTT
CCCTGAACGCGGTAAGCCAGAAAAAGAGGGAAGAGCTCATGCCCCTTACAAAAACGAACACCTTAGAAGAACTGATGGAA
GTTCTCAAAAACTACCCCCTTCCCAAGTACAGGAGGATTACCCTTGAGTACGTACTCATAAAGGGAGTAAACGACTCTCC
TAATGACGCGGAAAGGCTTGCAAAATTAATAGGAAGGCACAAAAAGAAGTTTAAAGTGAACTTAATTCCCTTTAACCCGG
ATCCTAACCTTCCCTACGAGAGACCCGCCCTCACCGACATCATGAAGTTCCAGAAGGTTTTATGGAAATATGGTATCTCT
AACTTCGTTAGGTTCAGCAAAGGCGTAGAGGTGTTCGGAGCCTGCGGTCAACTTAGAACTCAAAGGCTACAGCTCCAGAG
AGTATGA

Upstream 100 bases:

>100_bases
ACAGCCCTAGCACTCTTTTTAACCGCAGCTTTCTCTTCTCTGGTATTCCCGTGGAGTGCAAAAATAATTCTTTCTAGAAG
ATGAACTATATTCTGTAATT

Downstream 100 bases:

>100_bases
AAGTGGTAGAACTAAAAAACGTTAAAAAAATTTACCCCGACGGAACAATTGCTCTCAAAGGCGTTTCACTTTCCGTTGAA
GAGGGGGAATTTTTAGGCGT

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MEFVTNYTLEELKKRFTELGLEPYRAKQVFRWVYKKFVTDFEKMTDLGKKHRELLKEHFAFHPLEKLDRVEAPDAVKYLF
KTKDGHILETVLIKERDHYTLCVSSQIGCAVGCTFCATALDGLKRNLSTAEIIDQYLQVQQDLGEEKIRNVVFMGMGEPL
ANYENVRKAVEIMVSPEGLDLSKRRITISTSGIVAQIKRMAQDPVMKEVNLAVSLNAVSQKKREELMPLTKTNTLEELME
VLKNYPLPKYRRITLEYVLIKGVNDSPNDAERLAKLIGRHKKKFKVNLIPFNPDPNLPYERPALTDIMKFQKVLWKYGIS
NFVRFSKGVEVFGACGQLRTQRLQLQRV

Sequences:

>Translated_348_residues
MEFVTNYTLEELKKRFTELGLEPYRAKQVFRWVYKKFVTDFEKMTDLGKKHRELLKEHFAFHPLEKLDRVEAPDAVKYLF
KTKDGHILETVLIKERDHYTLCVSSQIGCAVGCTFCATALDGLKRNLSTAEIIDQYLQVQQDLGEEKIRNVVFMGMGEPL
ANYENVRKAVEIMVSPEGLDLSKRRITISTSGIVAQIKRMAQDPVMKEVNLAVSLNAVSQKKREELMPLTKTNTLEELME
VLKNYPLPKYRRITLEYVLIKGVNDSPNDAERLAKLIGRHKKKFKVNLIPFNPDPNLPYERPALTDIMKFQKVLWKYGIS
NFVRFSKGVEVFGACGQLRTQRLQLQRV
>Mature_348_residues
MEFVTNYTLEELKKRFTELGLEPYRAKQVFRWVYKKFVTDFEKMTDLGKKHRELLKEHFAFHPLEKLDRVEAPDAVKYLF
KTKDGHILETVLIKERDHYTLCVSSQIGCAVGCTFCATALDGLKRNLSTAEIIDQYLQVQQDLGEEKIRNVVFMGMGEPL
ANYENVRKAVEIMVSPEGLDLSKRRITISTSGIVAQIKRMAQDPVMKEVNLAVSLNAVSQKKREELMPLTKTNTLEELME
VLKNYPLPKYRRITLEYVLIKGVNDSPNDAERLAKLIGRHKKKFKVNLIPFNPDPNLPYERPALTDIMKFQKVLWKYGIS
NFVRFSKGVEVFGACGQLRTQRLQLQRV

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=356, Percent_Identity=39.6067415730337, Blast_Score=243, Evalue=1e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_AQUAE (O66732)

Other databases:

- EMBL:   AE000657
- PIR:   H70337
- RefSeq:   NP_213292.1
- ProteinModelPortal:   O66732
- GeneID:   1193058
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_416
- NMPDR:   fig|224324.1.peg.307
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- PhylomeDB:   O66732
- ProtClustDB:   PRK14467
- BioCyc:   AAEO224324:AQ_416-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 40250; Mature: 40250

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFVTNYTLEELKKRFTELGLEPYRAKQVFRWVYKKFVTDFEKMTDLGKKHRELLKEHFA
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
FHPLEKLDRVEAPDAVKYLFKTKDGHILETVLIKERDHYTLCVSSQIGCAVGCTFCATAL
CCCHHHHHCCCCCHHHHHHHHCCCCCEEHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHH
DGLKRNLSTAEIIDQYLQVQQDLGEEKIRNVVFMGMGEPLANYENVRKAVEIMVSPEGLD
HHHHHCCCHHHHHHHHHHHHHHHCHHHHHHEEEECCCCCHHHHHHHHHHHHHHCCCCCCC
LSKRRITISTSGIVAQIKRMAQDPVMKEVNLAVSLNAVSQKKREELMPLTKTNTLEELME
CCCCEEEEECHHHHHHHHHHHHCHHHHHHHHHEEEHHHHHHHHHHCCCCCCHHHHHHHHH
VLKNYPLPKYRRITLEYVLIKGVNDSPNDAERLAKLIGRHKKKFKVNLIPFNPDPNLPYE
HHHCCCCCCHHHEEEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCC
RPALTDIMKFQKVLWKYGISNFVRFSKGVEVFGACGQLRTQRLQLQRV
CHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEFVTNYTLEELKKRFTELGLEPYRAKQVFRWVYKKFVTDFEKMTDLGKKHRELLKEHFA
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
FHPLEKLDRVEAPDAVKYLFKTKDGHILETVLIKERDHYTLCVSSQIGCAVGCTFCATAL
CCCHHHHHCCCCCHHHHHHHHCCCCCEEHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHH
DGLKRNLSTAEIIDQYLQVQQDLGEEKIRNVVFMGMGEPLANYENVRKAVEIMVSPEGLD
HHHHHCCCHHHHHHHHHHHHHHHCHHHHHHEEEECCCCCHHHHHHHHHHHHHHCCCCCCC
LSKRRITISTSGIVAQIKRMAQDPVMKEVNLAVSLNAVSQKKREELMPLTKTNTLEELME
CCCCEEEEECHHHHHHHHHHHHCHHHHHHHHHEEEHHHHHHHHHHCCCCCCHHHHHHHHH
VLKNYPLPKYRRITLEYVLIKGVNDSPNDAERLAKLIGRHKKKFKVNLIPFNPDPNLPYE
HHHCCCCCCHHHEEEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCC
RPALTDIMKFQKVLWKYGISNFVRFSKGVEVFGACGQLRTQRLQLQRV
CHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320